Bitkilerde Rizosferden Demir Alım Mekanizmaları

Demir, toprakta en çok bulunan elementlerden bir tanesi olmasına karşın çözünürlüğü alkali topraklarda düşüktür. Dolayısıyla bu tür topraklarda yetişen bitkiler sürekli demir eksikliği stresine maruz kalırlar. Dünyadaki tarım arazilerin üçte biri bu tür topraklardan oluştuğundan dolayı tedavi edilemeyen demir eksikliği tarımsal üretimi kısıtlar. Bitkilerde gözlenen demir eksikliğinin tedavisinde farklı demir gübreleri kullanılmaktadır. Ancak, bu gübrelerin kullanımı üretim maliyetlerini artırmaktadır. Maliyetlerin azaltılabilmesi için bitkilerin toprakta bulunan demiri en etkin biçimde kullanabilmeleri gerekir. Bunun için de ilk olarak bitkilerin topraktaki demiri nasıl kök içerisine aldıklarının incelenmesi gerekmektedir. Son otuz yılda yapılan çalışmalarda farklı bitki gruplarının 3 farklı demir alım mekanizması kullandıkları keşfedilmiştir. Bu derlemenin amacı, demirin kök içerisine alımından sorumlu taşıyıcılar ile bu taşıyıcılar hakkındaki güncel gelişmelerden bahsetmektir

Bitkilerde Hücre İçi Demir Dağıtım Mekanizmaları

Temel mikro-besin elementi demir (Fe) bitkide önemli rolleri bulunan birçok metalloproteinin aktif bölgesinde kofaktör olarak yer alır. Öte yandan aşırı reaktif olduğundan, hücre içerisinde fazla birikimi reaktif oksijen türlerinin üretimini tetikleyerek hücre ölümlerine neden olur. Dolayısıyla hücre içerisindeki demir homeostazı bitki gelişimi için çok önemlidir. Bitkiler demiri kök içerisine aldıktan sonra hücre içi kompartmanlara dağıtımını yaparlar. Hücre içi demir taşınımı ve dolayısıyla hücresel demir homeostazı farklı membran protein ailelerinin senkronize kontrolü sayesinde yürütülmektedir. Bu membran proteinlerinin demir eksikliği altında ifade seviyelerinin arttığı keşfedilmiştir. Bu taşıyıcıların görev ve regülasyonlarının irdelenmesi bitkilerdeki demir alım ve dağıtım mekanizmalarının anlaşılması açısından çok önemlidir. Bu yüzden bu derlemede hücre içerisine alınan demirin organellere dağıtımından sorumlu taşıyıcılar ile bu taşıyıcılar hakkındaki güncel gelişmelerden bahsedilmektedir

The Antibody Response to Helicobacter pylori in the Sera from a Rural Population in the Central Anatolia Region of Turkey

WOS: 000261223600009Helicobacter pylori (H. pylori) infection is common worldwide. Although the seropositivity of H. pylori rates has been unclear in the Turkish population. In this study, anti-H. pylori IgG seroprevalence and anti-cytotoxin-associated gene A (CagA) IgG positivity were evaluated. The sera of 880 people without gastrointestinal symptoms (384 males, 496 females) were tested for anti-H. pylori IgG and anti-CagA IgG antibodies by enzyme linked immunoassay method. Anti-H. pylori IgG antibodies were positive in 263 sera (41%) and their rates increased with age. The seroprevalence of anti-H. pylori IgG was higher in females (43.8%) than in males (38%). Of the anti-H. pylori IgG positive sera, 194 (53%) were also positive for anti-CagA IgG. The anti-CagA IgG positivity did not significantly differ with age. However, the lowest rate (46.6%) was determined among individuals 2029 years of age and the highest rate (62.5%) among individuals over 60 years age. Anti-CagA IgG positivity rates were higher in males (87.5%) than in females (37.5%)

The Effect of Different Cooking Methods and Addition of Different Sweeteners on the Physicochemical and Antioxidant Properties of Aronia Marmalade

The present study examined the physicochemical properties, antioxidant activity (DPPH, ABTS, and FRAP), and sensory properties of aronia marmalades prepared with different cooking methods (CM) (boiled (B) and pressure-boiled (PB)) by adding sugar (S) and stevia prebiotic fiber sweetener (SP). Ash, reducing sugar, sucrose, viscosity, L*, a*, b*, C*, and H° values, and total sugar content of aronia pulp and marmalades differed significantly by cooking method and sweetener type (ST). Hydroxymethylfurfural could not be detected in aronia pulp and marmalades. Concerning CM, TPC (total phenolic content) and TMA (total monomeric anthocyanin) values were found to be significantly higher in PB cooking than in the B cooking method. On the other hand, TFC (total flavanoid content) was statistically higher in boiled marmalades. According to CM, the DPPH antioxidant activity of marmalades was significantly higher in B marmalades. The TPC, TMA, TFC, and antioxidant properties of marmalades differed sigficantly by ST. The TPC of marmalades prepared with SP addition was higher than that of S-added marmalades and control. According to ST, whereas the antioxidant activities (DPPH, ABTS, and FRAP) of S and SP-added marmalades were lower compared to the control, the antioxidant activities determined by DPPH and ABTS among S and SP-added marmalades were higher in SP-added marmalades. The panelists gave the highest scores to BSC (boiled S-added marmalade). Considering the overall acceptance scores, the second highest score was given to BST (boiled SP-added marmalade). In other words, in terms of sensory evaluation, boiled marmalades received higher overall acceptance scores, while PBST (PB SP-added marmalade) received the lowest scores. According to these results, astringency components decrease with cooking in an open vessel. Furthermore, it can be said that sugar masks this astringent taste

Subcellular Iron Localization Mechanisms in Plants

Temel mikro-besin elementi demir (Fe) bitkide önemli rolleri bulunan birçok metalloproteinin aktif bölgesinde kofaktör olarak yer alır. Öte yandan aşırı reaktif olduğundan, hücre içerisinde fazla birikimi reaktif oksijen türlerinin üretimini tetikleyerek hücre ölümlerine neden olur. Dolayısıyla hücre içerisindeki demir homeostazı bitki gelişimi için çok önemlidir. Bitkiler demiri kök içerisine aldıktan sonra hücre içi kompartmanlara dağıtımını yaparlar. Hücre içi demir taşınımı ve dolayısıyla hücresel demir homeostazı farklı membran protein ailelerinin senkronize kontrolü sayesinde yürütülmektedir. Bu membran proteinlerinin demir eksikliği altında ifade seviyelerinin arttığı keşfedilmiştir. Bu taşıyıcıların görev ve regülasyonlarının irdelenmesi bitkilerdeki demir alım ve dağıtım mekanizmalarının anlaşılması açısından çok önemlidir. Bu yüzden bu derlemede hücre içerisine alınan demirin organellere dağıtımından sorumlu taşıyıcılar ile bu taşıyıcılar hakkındaki güncel gelişmelerden bahsedilmektedir.The basic micro-nutrient element iron (Fe) is present as a cofactor in the active sites of many metalloproteins with important roles in the plant. On the other hand, since it is excessively reactive, excess accumulation in the cell triggers the production of reactive oxygen species, leading to cell death. Therefore, iron homeostasis in the cell is very important for plant growth. Once uptake into the roots, iron is distributed to the subcellular compartments. Subcellular iron transport and hence cellular iron homeostasis is carried out through synchronous control of different membrane protein families. It has been discovered that expression levels of these membrane proteins increase under iron deficiency. Examination of the tasks and regulations of these carriers is very important in terms of understanding the iron intake and distribution mechanisms in plants. Therefore, in this review, the transporters responsible for the uptake of iron into the cell and its subcellular distribution between organelles will be discussed with an emphasis on the current developments about these transporters

Investigation of Isolated Blastocystis Subtypes from Cancer Patients in Turkey

Aykur, Mehmet/0000-0002-6100-1037; aksoy, asude/0000-0002-5609-9658; Dalkilic, Semih/0000-0002-6892-247XWOS:000604203300004PubMed: 33387270Purpose It is not clear that Blastocystis remains without damage to the digestive tract or has a pathogenic effect in relation to subtypes in immunocompromised people, such as cancer patients. The present study aimed to investigate the frequency and subtype distribution of Blastocystis in cancer patients who were followed-up and treated in the Oncology clinic of Firat University Hospital and to determine the clinical signs of infected sufferers. Methods 201 patients aged >= 18 with a diagnosis of cancer were enrolled in this cross-sectional study. Patients' stool samples were examined between September 2017 and August 2019 by native-Lugol, trichrome staining. Microscopy-positive stool samples were subjected to DNA isolation and subtyped by Sequence Tagged Site (STS)-PCR analysis. The symptoms and demographic characteristics of the patients were also evaluated. Results Totally, 29 (14.4%) samples were positive for Blastocystis after all methods. 15 (51.7%) out of 29 samples were successfully subtyped by the sequenced-tagged site(STS)-PCR, while 14 (48.3%) could not be typed. Three subtypes of Blastocystis were detected: ST3 (40%), ST2 (33%), ST1 (20%), and one mixed infections with ST1/ST2 (6%). There was no statistically significant difference in terms of clinical findings and demographic characteristics. Conclusion The outcomes of our study promote the idea that Blastocystis could be an asymptomatic and harmless commensal organism. However, more comprehensive molecular and clinical studies are needed to fully determine the pathogenicity and epidemiology of Blastocystis in cancer patients.scientific and technological research council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [315S262]This work was funded by scientific and technological research council of Turkey (TUBITAK) (Grant 315S262)

Assessment of preoperative serum prolidase activity in epithelial ovarian cancer

Objective: Prolidase is a cytosolic exopeptidase that cleaves iminodipeptides with C-terminal proline and hydroxyproline and plays a major role in collagen turnover. The aim of this study was to evaluate preoperative serum prolidase activity, total oxidant status (TOS), and total antioxidant capacity (TAC) in patients with newly diagnosed epithelial ovarian cancer (EOC). Study design: Serum prolidase activity, TOS, and TAC were measured spectrophotometrically in both EOC cases (n = 28) and controls (n = 28). Results: Preoperative serum prolidase activity and TOS were significantly higher in patients with EOC compared to controls (p = 0.009 and 0.008, respectively), whereas TAC was significantly lower in cases (p < 0.001). A significant positive correlation was found between the stage, grade, and CA-125 level of EOC and serum prolidase activity (rho = 0.466, p = 0.012; rho = 0.501, p = 0.007; and rho = 0.447, p = 0.017, respectively) and TOS (rho = 0.588, p = 0.001; rho = 0.412, p = 0.029; and rho = 0.568, p = 0.002, respectively). In contrast, there was a negative correlation between TAC and the stage, grade, and CA-125 level of EOC (rho = -0.555, p = 0.002; rho = -0.566, p = 0.002; and rho = -0.581, p = 0.001, respectively). Conclusion: Findings of the present study demonstrate that serum prolidase activity is significantly associated with the stage, tumor grade, and preoperative CA-125 level of EOC. Crown Copyright © 2009

Serum prolidase activity and oxidative status in patients with stage I endometrial cancer

Introduction: We aimed to investigate the serum prolidase activity, total oxidant status (TOS), total antioxidant capacity (TAC), and total free sulfhydryl (-SH) groups in patients with stage I endometrial cancer. Methods: Serum prolidase activity, TOS, TAC, and -SH levels were measured in patients with endometrial cancer (n = 23) and controls (n = 25). Results: Serum prolidase activity and TOS were significantly higher in patients with endometrial cancer than in controls (P = 0.02 and P = 0.02, respectively), whereas TAC and -SH group levels were significantly lower (P = 0.004 and P = 0.001, respectively). There were no differences between surgical stages IA and IB or between stages IB and IC (P = 0.189 and P = 0.161, respectively) with respect to prolidase levels, whereas there was a significant difference between stages IA and IC (P < 0.001). Conclusions: Increased serum prolidase activity and oxidative stress may be associated with endometrial cancer, and increased serum prolidase activity may be related to local invasion of endometrial cancer. Copyright © 2009 by IGCS and ESGO

Serum prolidase activity and oxidative stress markers in pregnancies with intrauterine growth restricted infants

Aim: To compare the levels of serum prolidase activity and oxidative stress markers, including total oxidative status (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI), and total free sulfhydryl (-SH) in healthy pregnant women and pregnant women with intrauterine growth restricted infants. Methods: Serum samples were obtained from 18 healthy third trimester pregnancies and 14 pregnancies complicated by fetal growth restriction (FGR). The criteria for FGR were clinical evidence of suboptimal growth, ultrasonographic demonstration of deviation from normal percentiles of growth, and birthweight under the 10th percentile. We spectrophotometrically measured serum prolidase activity, TAC, TOS, and -SH levels. Oxidative stress was determined from total antioxidant capacity and total oxidant status measurement and calculation of the oxidative stress index. Results: Serum TAC and -SH levels were significantly lower in the FGR group than in the healthy control group (P = 0.001, P < 0.001, respectively), whereas TOS level, OSI value, and prolidase activity were significantly higher (P = 0.003, P < 0.001, P < 0.001, respectively). Prolidase activity was positively correlated with TOS and OSI values (rho = 0.552, P = 0.041 and rho = 0.635, P = 0.015, respectively) and negatively correlated with TAC and -SH levels (rho = -0.578, P = 0.030 and rho = -0.622, P = 0.018, respectively). Conclusion: The present study shows that serum prolidase activity and oxidative stress are significantly associated with the presence of FGR and that the correlation between serum prolidase activity and markers of oxidative stress are represented as increased serum TOS level and decreased serum TAC and -SH levels, suggesting an association of collagen turnover and oxidative stress in vascular dysfunction. © 2009 Japan Society of Obstetrics and Gynecology