Navigating Cultures, Forming Identities

This paper recounts the personal history of the Ang Family, focusing especially on three generations of women, starting with the family’s matriarch Carmen Davenport Barraca Ang, to her daughter Teresita Ang See, and lastly to Carmalea Yinching Ang See. Each life account brings out stories of bravery, creativity, and perseverance amidst hardships and challenges in a difficult environment. Carmen, for instance, was a Filipina married to a Chinese, who lived in China and then in Binondo. She endeared herself to her husband’s Chinese family by learning how to speak Hokkien fluently. Teresita Ang See attended conservative Chinese schools in Binondo but used her experience as a student of political science at the University of the Philippines in helping her late husband Chinben See found in the early 1970s Pagkakaisa Sa Pag-unlad; this was a non-government organization which fought anti-Chinese racism in the country. The story ends with Teresita’s children, Carmelea Yinching and Sean Benson, and how they were raised to be comfortable as Filipinos foremost, with the primary identity as Filipino and yet take pride in and promote the duality of their heritage. Through the narratives, the authors aim to give a glimpse of the complexity of identities and identity-formation, shaped as they were by the objective environment and the necessities of the times

The Fires of Revolution: Shared History, Shared Destiny

This lecture underscores the mutual help and support that existed between the Chinese and the Filipinos as early as the 19th century. Chinese records, for example, are replete with writings of Chinese revolutionaries in praise and admiration of the Filipino revolutionaries who dared to fight not just one but two foreign white colonizers. This shared history reflects even more clearly the closeknit relations between the Filipinos and the Chinese as well as the common history and a common destiny they shared

Toward a History of Chinese Burial Grounds in Manila during the Spanish Colonial Period

The study of the Chinese burial grounds in Manila provides a window to the world of the Chinese community and the Spanish colonial regime. In the first two and a half centuries of Spanish rule, the Chinese were buried in church or temple grounds, demonstrating the prerogative of religious authorities over the lives of these colonial subjects. In the nineteenth century, the rise of the Chinese population and new approaches to public sanitation paved the way for the establishment of an extramural Chinese public cemetery. The regulations, and the issues that came with administering this cemetery reflect how Chinese elite interests intersected with attempts by a declining colonial power to assert control produced a cemetery like no other in Southeast Asia.L’étude des sites funéraires de Manille ouvre de nouveaux horizons sur la communauté chinoise et le régime colonial espagnol. Dans les deux premiers siècles et demi que dura cette domination, les Chinois ont été ensevelis à l’intérieur ou autour des églises ou encore sur les terrains des temples, démontrant la prérogative des autorités religieuses sur la vie de ces sujets coloniaux. Au cours du xixe siècle, l’augmentation de la population chinoise et les nouvelles conceptions de l’hygiène publique ont ouvert la voie à la création d’un cimetière public chinois extra-muros. Les règlements et les problèmes auxquels ont été confrontés les administrateurs de ce site funéraire révèlent que les intérêts de l’élite chinoise ont recoupé les ultimes tentatives de contrôle de la puissance coloniale, donnant ainsi naissance à un cimetière unique en Asie du Sud-Est

Degradation of histamine by the halotolerant Staphylococcus carnosus FS19 isolate obtained from fish sauce

Histamine is found in many fermented food products and may have detrimental effects on the health of its consumers. Histamine and other amines are degraded by the oxidative deamination activity of certain microorganisms. In this study, the growth characteristics and histamine-degrading activity of a Staphylococcus carnosus FS19 isolate derived from fish sauce were investigated. This bacterium exhibits optimal growth at 35 °C, pH 8 and 9% sodium chloride when cultivated in tryptic soy broth. The histamine-degrading activity of the S. carnosus FS19 isolate was optimised at 40 °C and pH 6 in 9% buffered sodium chloride. When added to fish sauce samples, this bacterium exhibits remarkable histamine-degrading activity. The histamine concentration was reduced by approximately 15.1% and 13.8% in the fish sauce samples that contained 18% and 21% salt, respectively. However, no histamine degradation was observed in samples with a salt content greater than 21%. In addition, a slight degradation of other amines, including putrescine and cadaverine, was also observed in some of the samples. In contrast, tyramine degradation did not occur in any of the samples. Therefore, S. carnosus FS19 is a culture that could potentially reduce the histamine content of fermented fish products

Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells.

Notch-1 receptor signaling pathway is involved in neuronal and glial differentiation. Its involvement in microglial functions, however, has remained elusive. This study reports the localization of Notch-1 receptor immunoreactivity in the amoeboid microglial cells (AMC) in the postnatal rat brain. By immunofluorescence, Notch-1 receptor was colocalized with its ligands, Jagged-1 and Delta-1, in the AMC in the corpus callosum and subventricular zone. Notch-1 immunopositive cells were confirmed to be microglia labeled by OX42 and lectin. Immunoexpression of Notch-1 receptor was progressively reduced with age. Western blot analysis showed that Notch-1 protein level in the corpus callosum in which the AMC were heavily populated was concomitantly decreased. In postnatal rats challenged with lipopolysaccharide (LPS), Notch-1 receptor immunofluorescence in AMC was noticeably enhanced. Furthermore, Notch-1 protein level in the corpus callosum was increased as revealed by Western blotting analysis. In primary microglial culture treated with LPS, mRNA expression of Notch-1 and its ligand Jagged-1 was upregulated but that of Delta-1 was reduced. The expression pattern of Notch-1 and its ligands was confirmed in murine BV-2 cells. Furthermore, Notch-1 neutralization with its antibody reduced its protein expression. More importantly, neutralization of Notch-1 concomitantly suppressed the mRNA expression of IL-6, IL-1, M-CSF, and iNOS; TNF-α, mRNA expression, however, was enhanced. Western blot confirmed the changes of protein level of the above except for IL-6, which remained relatively unaltered. It is concluded that Notch-1 signaling in the AMC and LPS-activated microglia/BV-2 cells modulates the expression of proinflammatory cytokines and nitric oxide

Directed evolution and predictive modelling of galactose oxidase towards bulky benzylic and unactivated secondary alcohols

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Multicultural mediations, developing world realities: Indians, Koreans and Manila’s entertainment media

In this article, I examine the mediation of multiculturalism in the developing world city of Manila, the Philippines. Drawing on both a thematic analysis of the Manila-centric Philippine entertainment media and six focus group discussions with the city’s local Filipinos, I reveal that this instance of mediation is entangled with the broader discourses of the Philippine postcolonial nationalist project. For one, the mediation of multiculturalism in Manila tends to symbolically marginalize the city’s Indians and Koreans and, in so doing, reinforces existing negative discourses about them. I contend that this is linked to the locals’ preoccupation with establishing a unifying cultural identity that tends to make them elide the issue of their own internal cultural diversity, as well as of the increasing diasporic population of the city. Second, the said mediation also tends to valorize the lighter-skinned Koreans over the darker-skinned Indians. I posit that this is related to how the locals’ discourse of cultural homogeneity has resulted in their continued reluctance to publicly discuss the persistence of their unspoken skin-tone-based racial hierarchy not only of themselves, but also of their cultural others

Automated Machine Learning (AutoML)-Derived Preconception Predictive Risk Model to Guide Early Intervention for Gestational Diabetes Mellitus

The increasing prevalence of gestational diabetes mellitus (GDM) is contributing to the rising global burden of type 2 diabetes (T2D) and intergenerational cycle of chronic metabolic disorders. Primary lifestyle interventions to manage GDM, including second trimester dietary and exercise guidance, have met with limited success due to late implementation, poor adherence and generic guidelines. In this study, we aimed to build a preconception-based GDM predictor to enable early intervention. We also assessed the associations of top predictors with GDM and adverse birth outcomes. Our evolutionary algorithm-based automated machine learning (AutoML) model was implemented with data from 222 Asian multi-ethnic women in a preconception cohort study, Singapore Preconception Study of Long-Term Maternal and Child Outcomes (S-PRESTO). A stacked ensemble model with a gradient boosting classifier and linear support vector machine classifier (stochastic gradient descent training) was derived using genetic programming, achieving an excellent AUC of 0.93 based on four features (glycated hemoglobin A(1c) (HbA(1c)), mean arterial blood pressure, fasting insulin, triglycerides/HDL ratio). The results of multivariate logistic regression model showed that each 1 mmol/mol increase in preconception HbA(1c) was positively associated with increased risks of GDM (p = 0.001, odds ratio (95% CI) 1.34 (1.13-1.60)) and preterm birth (p = 0.011, odds ratio 1.63 (1.12-2.38)). Optimal control of preconception HbA(1c) may aid in preventing GDM and reducing the incidence of preterm birth. Our trained predictor has been deployed as a web application that can be easily employed in GDM intervention programs, prior to conception.Peer reviewe

Cryopreservation of Neurospheres Derived from Human Glioblastoma Multiforme

Cancer stem cells have been shown to initiate and sustain tumor growth. In many instances, clinical material is limited, compounded by a lack of methods to preserve such cells at convenient time points. Although brain tumor-initiating cells grown in a spheroid manner have been shown to maintain their integrity through serial transplantation in immune-compromised animals, practically, it is not always possible to have access to animals of suitable ages to continuously maintain these cells. We therefore explored vitrification as a cryopreservation technique for brain tumor-initiating cells. Tumor neurospheres were derived from five patients with glioblastoma multiforme (GBM). Cryopreservation in 90% serum and 10% dimethyl sulfoxide yielded greatest viability and could be explored in future studies. Vitrification yielded cells that maintained self-renewal and multipotentiality properties. Karyotypic analyses confirmed the presence of GBM hallmarks. Upon implantation into NOD/SCID mice, our vitrified cells reformed glioma masses that could be serially transplanted. Transcriptome analysis showed that the vitrified and nonvitrified samples in either the stem-like or differentiated states clustered together, providing evidence that vitrification does not change the genotype of frozen cells. Upon induction of differentiation, the transcriptomes of vitrified cells associated with the original primary tumors, indicating that tumor stem-like cells are a genetically distinct population from the differentiated mass, underscoring the importance of working with the relevant tumor-initiating population. Our results demonstrate that vitrification of brain tumor-initiating cells preserves the biological phenotype and genetic profiles of the cells. This should facilitate the establishment of a repository of tumor-initiating cells for subsequent experimental designs

The pole structure of the unitary, crossing symmetric low energy ππ\pi\pi scattering amplitudes

The pole structure of the low energy $\pi\pi$ scattering amplitudes is studied using a proper chiral unitarization method combined with crossing symmetry and the low energy phase shift data. It is found that the $\sigma$ pole position is at $M_\sigma=470\pm 50MeV$, $\Gamma_\sigma=570\pm 50MeV$. The existence of the virtual state pole in the IJ=20 channel is reconfirmed. Various threshold parameters are estimated and are found in general in good agreement with the results obtained from the Roy equation analyses.Comment: Minor corrections made and references added. Final version accepted for publication as JHEP02(2005)04