Disseny d'un carret de compra per a l'ús personal amb compartiments modulars

[ES] En el presente trabajo se ha llevado a cabo el diseño de un carrito de compra cuya función principal es el almacenamiento y transporte de productos de supermercado. Para ello, se han seguido los pasos metodológicos clásicos del proceso de diseño, que incluyen la identificación de la necesidad potencial, el análisis del problema, la definición de los requerimientos de diseño, la generación de alternativas, la propuesta conceptual, el diseño técnico y el prototipado. La solución desarrollada se caracteriza por el diseño modular de los compartimentos del carrito los cuales se pueden separar.[EN] In this work is presented the design of a shopping cart whose main function is the storage and transport of supermarket products. For this, the classic methodological steps of the design process have been followed, which include the identification of the potential need, the problem analysis, the definition of the design requirements, the generation of alternatives, the conceptual proposal, the technical design and the prototyping. The developed solution is characterized by the modular design of the trolley compartments which can be separated.Szafranski Olejniczak, SS. (2023). Design of a shopping cart for personal use with modular compartments. Universitat Politècnica de València. http://hdl.handle.net/10251/19701

Intrinsic gain switching in optically injected quantum dot laser lasing simultaneously from the ground and excited state

International audienceWe analyze theoretically nonlinear dynamics of an optically injected two-mode quantum dot laser lasing simultaneously from the ground and excited states. We show that although the external optical signal is injected into the ground-state mode alone, it can lead to the generation of regular picosecond pulses and pulse packages in the intensity of the excited-state mode. Generation of regular streams of picosecond pulses is attributed to an intrinsic gain switching mechanism where the relaxation time is modulated by the oscillations in the occupation of the ground and excited energy state

Test Harness on a Preconditioned Conjugate Gradient Solver on GPUs: An Efficiency Analysis

International audienceThe parallelization of numerical simulation algo- rithms, i.e., their adaptation to parallel processing architectures, is an aim to reach in order to hinder exorbitant execution times. The parallelism has been imposed at the level of processor ar- chitectures and graphics cards are now used for general-purpose calculation, also known as "General-Purpose computation on Graphics Processing Unit (GPGPU)". The clear benefit is the excellent performance over price ratio. Besides hiding the low level programming, software engineering leads to a faster and more secure application development. This paper presents the real interest of using GPU processors to increase performance of larger problems which concern electrical machines simulation. Indeed, we show that our auto-generated code applied to several models allows achieving speedups of the order of 10x

Search of structure and ligands exchange for palladium(II) complexes with N-allylimidazole; X-ray and solid-state/solution NMR studies

Two coordination compounds of palladium(II) with N-allylimidazole ((L)) of the general formula [PdL(4)]Cl(2) center dot 3H(2)O (1) and trans-[PdL(2)Cl(2)] (2) have been synthesized. The crystal and molecular structure of complexes 1 and 2 was established by single-crystal X-ray diffraction analysis. The X-ray structural data were supplemented by solid-state (13)C NMR measurements (CP MAS and PASS 2D). The 1D and 2D NMR studies in solution reveal that complex 1 is unstable at room temperature and undergoes reversible decomposition to 2. The method for how to preserve a complex with four allyl-imidazole ligands in solution is shown. (c) 2005 Elsevier B.V. All rights is reserved

Insight into key structural points of capsule-targeting phage depolymerases specific to Klebsiella pneumoniae K63

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Cytotoxic effects of halophilic archaea metabolites on ovarian cancer cell lines

Abstract Background Ovarian cancer is one of the most frequent and deadly gynaecological cancers, often resistant to platinum-based chemotherapy, the current standard of care. Halophilic microorganisms have been shown to produce a large variety of metabolites, some of which show toxicity to various cancer cell lines. However, none have yet been shown to be active against ovarian cancer cells. Here, we examined the effects of metabolites secreted by the halophilic archaea Halorhabdus rudnickae and Natrinema salaciae on various cancer cell lines, including ovarian cancer cell lines. Results 1H NMR analyses of Hrd. rudnickae and Nnm. salaciae culture supernatants contain a complex mixture of metabolites that differ between species, and even between two different strains of the same species, such as Hrd. rudnickae strains 64T and 66. By using the MTT and the xCELLigence RTCA assays, we found that the secreted metabolites of all three halophilic strains expressed cytotoxicity to the ovarian cancer cell lines, especially A2780, as well as its cisplatin-resistant derivative A2780cis, in a dose-dependent manner. The other tested cell lines A549, HepG2, SK-OV-3 and HeLa were only minimally, or not at all affected by the archaeal metabolites, and this was only seen with the MTT assay. Conclusions The halophilic archaea Hrd. rudnickae and Nnm. salaciae, isolated from a Polish salt mine and Lake Medee in the Mediterranean Sea, respectively, secrete metabolites that are active against ovarian cancer cells, including those that are resistant to cisplatin. This opens potential new possibilities for the treatment of these frequent and deadly gynaecological cancers

Klebsiella phage KP34gp57 capsular depolymerase structure and function: from a serendipitous finding to the design of active mini-enzymes against K. pneumoniae

ABSTRACT Virion-associated depolymerases are large trimeric and multi-domain proteins that constitute the phage arsenal to degrade the polysaccharide layers in their bacterial host. Thus, as recombinant proteins, they are endowed with huge potential in biotechnology and medicine. In this study, we elucidated the structural and functional features of the capsular depolymerase KP34gp57 from the Klebsiella phage KP34. Based on the crystal structure and site-directed mutagenesis, we localized the key catalytic residues in an intra-subunit deep groove. Moreover, we engineered several N- and C-terminally truncated versions of KP34gp57 to dissect the role of each domain in the enzyme’s stability and catalytic activity. Serendipitously, our studies revealed C-terminally trimmed KP34gp57 variants that did not trimerize and were sufficiently stable to preserve full catalytic activity as monomers. The elaboration of trimmed monomeric and fully active phage depolymerases is innovative in the field, as no previous example exists apart from bacterial enzymes. Mini phage depolymerases can be optionally combined within chimeric enzymes to extend their activity range, facilitating their use in stand-alone treatments. Moreover, the intra-subunit and inter-subunit locations of the catalytic pocket in phage depolymerases might suggest differences in their evolutionary origin. IMPORTANCE In this work, we determined the structure of Klebsiella phage KP34p57 capsular depolymerase and dissected the role of individual domains in trimerization and functional activity. The crystal structure serendipitously revealed that the enzyme can exist in a monomeric state once deprived of its C-terminal domain. Based on the crystal structure and site-directed mutagenesis, we localized the key catalytic residues in an intra-subunit deep groove. Consistently, we show that C-terminally trimmed KP34p57 variants are monomeric, stable, and fully active. The elaboration of monomeric, fully active phage depolymerases is innovative in the field, as no previous example exists. Indeed, mini phage depolymerases can be combined in chimeric enzymes to extend their activity ranges, allowing their use against multiple serotypes