A MegaCam Survey of Outer Halo Satellites. VII. A Single S\'ersic Index v/s Effective Radius Relation for Milky Way Outer Halo Satellites

In this work we use structural properties of Milky Way's outer halo ($R_G > 25\,\mathrm{kpc}$) satellites (dwarf spheroidal galaxies, ultra-faint dwarf galaxies and globular clusters) derived from deep, wide-field and homogeneous data, to present evidence of a correlation in the S\'ersic index v/s effective radius plane followed by a large fraction of outer halo globular clusters and satellite dwarf galaxies. We show that this correlation can be entirely reproduced by fitting empirical relations in the central surface brightness v/s absolute magnitude and S\'ersic index v/s absolute magnitude parameter spaces, and by assuming the existence of two types of outer halo globular clusters: one of high surface brightness (HSB group), with properties similar to inner halo clusters; and another of low surface brightness (LSB group), which share characteristics with dwarf spheroidal and ultra-faint dwarf galaxies. Given the similarities of LSB clusters with dwarf spheroidal and ultra-faint dwarf galaxies, we discuss the possibility that outer halo clusters also originated inside dark matter halos and that tidal forces from different galaxy host's potentials are responsible for the different properties between HSB and LSB clusters.Comment: 20 pages, 9 figures, 3 table

A completely phased diploid genome assembly for "Malbec" cultivar (Vitis vinifera L.)

Most grapevine cultivars originated from the outcrossing of two genetically diverse parents, and are clonally propagated to preserve phenotypes of productive interest. Hence, cultivars are first filial generations (F1) with highly heterozygous diploid genomes, that turn challenging to assemble. "Malbec" is the main cultivar for the Argentine wine industry and it originated in France, from the outcrossing of Magdeleine Noir des Charentes and Prunelard cultivars. Based on that mother-father-offspring relationship, here we followed the algorithm implemented in the software CanuTrio to produce a phased assembly of Malbec genome. For this aim, parental cultivars? Illumina short-reads were used to sort ?Malbec? PacBio long-reads into its haploid complements, to be assembled separately. Post- assembly, bioinformatic procedures were employed to reduce the number of duplicated regions and perform sequence error corrections (using Malbec Illumina short-reads). We obtained two highly complete and contiguous haploid assemblies for Malbec, Haplotype- Prunelard (482.4 Mb size; contig N50=7.7 Mb) and Haplotype-Magdeleine (479.4 Mb size; contig N50=6.6 Mb), with 96.1 and 95.8% of BUSCO genes, respectively. We tested for the composition of both haplophases with the tool Merqury, and observed 15% of both assemblies affected by structural variations, along with 3.2 million SNPs and 0.6 million InDels. Our results indicate that this is a valid approach to assemble highly heterozygous and complex diploid genomes in a completely-phased way.Fil: Calderón, Pablo Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Carbonell Bejerano, P.. Max Planck Institute for Developmental Biology; AlemaniaFil: Mauri, N. Instituto de Ciencias de la Vid y del Vino; EspañaFil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Bree, Laura. No especifíca;Fil: Sola, C. No especifíca;Fil: Bergamin, D. No especifíca;Fil: Gómez Talquenca, Sebastián. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Ibañez, J. Instituto de Ciencias de la Vid y del Vino; EspañaFil: Martinez-Zapater, JM. Instituto de Ciencias de la Vid y del Vino; EspañaFil: Lijavetzky, Diego Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaXVIII Congreso Latinoamericano de Genética; LIV Reunión Anual de la Sociedad de Genética de Chile; XLIX Congreso Argentino de Genética; VIII Congreso de la Sociedad Uruguaya de Genética; I Congreso Paraguayo de Genética y V Congreso Latinoamericano de Genética HumanaChileSociedad Argentina de Genétic

International fisheries threaten globally endangered sharks in the Eastern Tropical Pacific Ocean: the case of the Fu Yuan Yu Leng 999 reefer vessel seized within the Galápagos Marine Reserve

Shark fishing, driven by the fin trade, is the primary cause of global shark population declines. Here, we present a case study that exemplifies how industrial fisheries are likely depleting shark populations in the Eastern Tropical Pacific Ocean. In August 2017, the vessel Fu Yuan Yu Leng 999, of Chinese flag, was detained while crossing through the Galápagos Marine Reserve without authorization. This vessel contained 7639 sharks, representing one of the largest seizures recorded to date. Based on a sample of 929 individuals (12%), we found 12 shark species: 9 considered as Vulnerable or higher risk by the IUCN and 8 listed in CITES. Four species showed a higher proportion of immature than mature individuals, whereas size-distribution hints that at least some of the fishing ships associated with the operation may have been using purse-seine gear fishing equipment, which, for some species, goes against international conventions. Our data expose the magnitude of the threat that fishing industries and illegal trade represent to sharks in the Eastern Tropical Pacific Ocean

Pesticides in soil, groundwater and food in Latin America as part of one health.

We here report of a conference about "Pesticides in Soil, Groundwater and Food in Latin America as part of One Health" that took place at the "IV Seminario Internacional de Sanidad Agropecuaria (SISA)" in Varadero, Cuba, 8-12 May 2023. Researchers of Latin America (Argentina, Brazil, Chile, Costa Rica, Colombia, Cuba, Mexico) and Switzerland (workshop initiator) held presentations about occurrence and effects of pesticides on the environment, human health, the replacement of highly hazardous pesticides (HHP) by agroecological alternatives and the agri-food value chain. In a subsequent round table discussion, the presenters identified deficits, needs, interests and opportunities. According to them, the lack of awareness of pesticide use affects the health and safety of workers applying the chemicals. Despite Latin America representing the main agricultural area in the world with a very intense pesticide use, monitoring data of pesticides in soil, surface and groundwaters, food, as well as in humans are missing. Risks of pesticides to humans should be assessed so that authorities can withdraw or limit within "short time" the access to corresponding formulations on the market. Also, communication is not state of the art and should be improved as, e.g. the teaching of workers and farmers, how to correctly use and apply pesticides or the briefing of decision makers. Pollinators suffer from multiple stressors not the least due to pesticides, and alternatives are badly needed. On the technical side, the different analytical methods to determine residues of active ingredients and transformation products in matrices of concern should be harmonized among laboratories.Seven future actions and goals were identified to overcome the above deficits. Next steps after the publishing of this conference report are to harmonize and complete the information status of the presenters by exchanging the results/data already present. Therefore, a platform of interaction to address issues described above and to enhance collaboration shall be created. Samples of different matrices shall be exchanged to harmonize the chemical analysis and establish interlaboratory comparisons. Such activities might be facilitated by joining international associations or organizations, where researchers can offer their expertise, or by forming a new pesticide network for Central and South America that could present tailored projects to national and international organizations and funding agencies

New techniques to improve fertigation efficiency with nitrification inhibitors in lettuce (Lactuca sativa L.) in Murcia

[SPA] El nitrógeno (N) es un elemento esencial para el crecimiento de las plantas y es el nutriente que se suele aplicar en mayor cantidad en la agricultura, pero se puede perder una cantidad significativa por la lixiviación, emisiones gaseosas y volatilización de NH3; aumentando los costos de producción e impactando negativamente sobre el medio ambiente. Los inhibidores de la nitrificación (IN) permiten reducir estas pérdidas, simplificar la tarea de fertilización, disminuir costos y facilitar una mayor flexibilidad en el tiempo de aplicación. La problemática esencial de los cultivos hortícolas en Murcia es el manejo optimizado de agua y nutrientes por lo que la incorporación de IN debe someterse a protocolos que aún no están definidos en la agricultura intensiva. El objetivo general de la investigación es elaborar desde un punto de vista multidisciplinar criterios eficientes y sostenibles para el manejo de la fertirrigación de lechuga mediante el uso de fertilizantes con IN y la monitorización del sistema suelo-planta-atmósfera, para mejorar la eficiencia del uso del agua y nutrientes, cumplir los estándares de calidad exigidos para la exportación y disminuir la emisión de gases de efecto invernadero según las condicionantes edafoclimáticas locales de Murcia. [ENG] Nitrogen (N) is an essential element for the growth of plants and is the nutrient that is usually applied in greater quantity in agriculture, but a significant amount can be lost through leaching, gaseous emissions and volatilization of NH3; increasing production costs and negatively impacting the environment. The nitrification inhibitors (NI) allow reducing these losses, simplifying the fertilization management, reducing costs and facilitating greater flexibility in the time of application. The essential problem of horticultural crops in Murcia is the optimized management of water and nutrients, so the incorporation of NI must be subject to protocols that are not yet defined in intensive agriculture. The general objective of the research is to develop, from a multidisciplinary point of view, efficient and sustainable criteria for the management of lettuce fertigation through the use of NI fertilizers and the monitoring of the soil-plant-atmosphere system, to improve the efficiency of the use of water and nutrients, meet the quality standards required for export and reduce the emission of greenhouse gases according to the local edaphoclimatic conditions of Murcia

A MegaCam Survey of Outer Halo Satellites. VII. A Single Sérsic Index versus Effective Radius Relation for Milky Way Outer Halo Satellites

In this work, we use structural properties of the Milky Way's outer halo (R_G > 25 kpc) satellites (dwarf spheroidal galaxies, ultra-faint dwarf (UFD) galaxies and globular clusters (GCs)) derived from deep, wide-field, and homogeneous data to present evidence of a correlation in the Sérsic index versus effective radius plane followed by a large fraction of outer halo GCs and satellite dwarf galaxies. We show that this correlation can be entirely reproduced by fitting empirical relations in the central surface brightness versus absolute magnitude and Sérsic index versus absolute magnitude parameter spaces, and by assuming the existence of two types of outer halo GCs: one of high surface brightness (HSB group), with properties similar to those of inner halo clusters; and another of low surface brightness (LSB group), which share characteristics with dwarf spheroidal and UFD galaxies. Given the similarities of LSB clusters with dwarf spheroidal and UFD galaxies, we discuss the possibility that outer halo clusters also originated inside dark matter halos and that tidal forces from different host galaxy potentials are responsible for the different properties between HSB and LSB clusters

El ASIS en el contexto de la intervención comunitaria y su rol pedagógico: Redes intersectoriales en el Barrio Nuevo de Ringuelet (La Plata, 2018-2019)

El presente trabajo tiene como objetivo sistematizar y comunicar los resultados del desarrollo de un Análisis de Situación Integral de Salud (ASIS) en el Barrio Nuevo de Ringuelet (La Plata) -territorio surgido a partir de una relocalización de asentamientos populares- entre los meses de julio de 2018 y octubre de 2019. Como Residencia de Medicina General del Hospital Gutiérrez del Centro de Atención Primaria de la Salud (CAPS) n° 15, la pregunta de la que partimos es la siguiente: ¿Cuáles son las características sociodemográficas y las problemáticas de salud de la población que habita en Barrio Nuevo después de la relocalización? El propósito de esta investigación es conocer a la población, sus problemáticas, necesidades y deseos para incidir en la calidad de vida de la misma desde una perspectiva sanitaria. Para el equipo de trabajo esto significa acompañar procesos participativos y colectivos en vistas de transformar la situación de salud del territorio partiendo de sus propias configuraciones culturales. El objetivo general fue realizar un análisis de situación de salud en el Barrio Nuevo de La Plata para identificar y priorizar problemáticas desde un enfoque de derechos, participativo e intersectorial durante el período 2018-2019.Dirección de Redes Intersectoriales en Salud de la Universidad Nacional de La Plat

FACT Prevents the Accumulation of Free Histones Evicted from Transcribed Chromatin and a Subsequent Cell Cycle Delay in G1

The FACT complex participates in chromatin assembly and disassembly during transcription elongation. The yeast mutants affected in the SPT16 gene, which encodes one of the FACT subunits, alter the expression of G1 cyclins and exhibit defects in the G1/S transition. Here we show that the dysfunction of chromatin reassembly factors, like FACT or Spt6, down-regulates the expression of the gene encoding the cyclin that modulates the G1 length (CLN3) in START by specifically triggering the repression of its promoter. The G1 delay undergone by spt16 mutants is not mediated by the DNA–damage checkpoint, although the mutation of RAD53, which is otherwise involved in histone degradation, enhances the cell-cycle defects of spt16-197. We reveal how FACT dysfunction triggers an accumulation of free histones evicted from transcribed chromatin. This accumulation is enhanced in a rad53 background and leads to a delay in G1. Consistently, we show that the overexpression of histones in wild-type cells down-regulates CLN3 in START and causes a delay in G1. Our work shows that chromatin reassembly factors are essential players in controlling the free histones potentially released from transcribed chromatin and describes a new cell cycle phenomenon that allows cells to respond to excess histones before starting DNA replication

Human Endometrial CD98 Is Essential for Blastocyst Adhesion

BACKGROUND: Understanding the molecular basis of embryonic implantation is of great clinical and biological relevance. Little is currently known about the adhesion receptors that determine endometrial receptivity for embryonic implantation in humans. METHODS AND PRINCIPAL FINDINGS: Using two human endometrial cell lines characterized by low and high receptivity, we identified the membrane receptor CD98 as a novel molecule selectively and significantly associated with the receptive phenotype. In human endometrial samples, CD98 was the only molecule studied whose expression was restricted to the implantation window in human endometrial tissue. CD98 expression was restricted to the apical surface and included in tetraspanin-enriched microdomains of primary endometrial epithelial cells, as demonstrated by the biochemical association between CD98 and tetraspanin CD9. CD98 expression was induced in vitro by treatment of primary endometrial epithelial cells with human chorionic gonadotropin, 17-β-estradiol, LIF or EGF. Endometrial overexpression of CD98 or tetraspanin CD9 greatly enhanced mouse blastocyst adhesion, while their siRNA-mediated depletion reduced the blastocyst adhesion rate. CONCLUSIONS: These results indicate that CD98, a component of tetraspanin-enriched microdomains, appears to be an important determinant of human endometrial receptivity during the implantation window