Detailed reconstruction and safety analysis of a pre–Seveso accident

Industrial safety has been a topic of growing interest during the last decades, mainly because of the increased awareness and knowledge about safety issues. In this framework, the detailed reconstruction of the dynamics of an explosion (1 killed and 8 injured) occurred, on the 26th of June 1971, at Noury Italy (a plant dedicated to the production of chemicals for hardening plastics) is worth of interest and it could be used to improve actual safety guidelines related to the storage of peroxides. The accident happened before whatever Seveso Directive release. Therefore, root-causes reconstruction and related risk assessment were carried out making a comparison between a hypothetical plant layout at that time and a modern plant layout implemented with minimum safety systems, such as acoustic alarms and adequate bypass lines. The accident reconstruction was carried out by doing a deep literature research, mainly based on newspaper clippings of the time, to both remodel the accident at best and draw the most likely layout of the plant. The latter is of fundamental importance to carry out a risk assessment procedure by applying the Recursive Operability Analysis (ROA), which allows for a direct generation of the fault trees that can provide an easy estimation of the probability of occurrence of all unwanted events. This method was applied to the Noury Italy case study to show the criticalities of the storage equipment also underlining the possible improvements which could be implemented also in the ‘70s, therefore preventing the fatal explosion

Recursive operability analysis as a tool for ATEX classification in plants managing explosive dusts

Safety and prevention in workplaces are important issues, especially regards to risks with serious consequences for health and infrastructures, such as dust explosions, which have caused several industrial accidents during the last centuries and, actually, represent a critical issue in the industrial framework. The current European legislation, referred to as ATEX directive, identifies ATEX zones as parts of the plant where explosive atmospheres can be generated. In this work, a modified version of the classic Recursive Operability Analysis method, specifically tailored to define with an automatic procedure the ATEX zones related to flammable dust clouds, is proposed. The method is fast and effective, allowing for an automatic generation of fault trees from which the probability of occurrence defining the specific ATEX zone type can be estimated. This technique was successfully implemented in a chemical plant dedicated to the mixing of inert powders with a stearate powder, a hazardous dust classified as strongly explosible. The extent of all the ATEX zones identified within the plant was simulated with the ALOHA software, treating the dispersed dust cloud of stearate as a dense gas cloud. From the results, it was possible to identify not only type and extension of all the ATEX zones but also either the most critical parts of the plant or the most dangerous activities (e.g. human errors in the use of the forklift was found to account for about 97.7% to explosion probability in this type of plant)

A mathematical model for the prediction of the KSt for metallic dusts as a function of the particle size distribution

For several years, dust explosions have been one of the major causes of industrial accidents, spanning from metalworking to pharmaceuticals sectors. In accordance with the latest Chemical Safety Board (CSB) investigations, three out of four dust explosions in the United States involved metallic dusts (iron, titanium, zirconium and aluminum). Many chemical processes involve metal powders for their exceptional mechanical, optical and catalytic properties, such as the production of plastics, rubber, paints, coatings, inks, pesticides, detergents and even drugs. The severity of a dust explosion can be defined using experimental parameters such as the maximum explosion pressure (pmax), the maximum rate of pressure rise ((dp/dt)max) and the deflagration index (Kst), which are employed to predict the consequences of a dust explosion for a given scenario. Among these parameters, the deflagration index plays a fundamental role, as it is used for the design of deflagration nozzles aimed to protect industrial equipment and silos from internal dust explosions. The purpose of this work is to develop a mathematical model able to predict the Kst value of metal powders as a function of chemical-physical data and the particle size distribution (DD50 was used as global information). The model structure is based on the writing and resolution of the material and energy balance equations on the single dust particle, also estimating the contribution of oxygen diffusion which, in the case of metal powders, greatly depends on both tortuosity and porosity. The results well agreed with experimental data, providing the basis for the development of more detailed models

Platelet-derived transforming growth factor-β1 promotes keratinocyte proliferation in cutaneous wound healing.

Platelets are a recognised potent source of transforming growth factor-β1 (TGFβ1), a cytokine known to promote wound healing and regeneration by stimulating dermal fibroblast proliferation and extracellular matrix deposition. Platelet lysate has been advocated as a novel personalised therapeutic to treat persistent wounds, although the precise platelet-derived growth factors responsible for these beneficial effects have not been fully elucidated. The aim of this study was to investigate the specific role of platelet-derived TGFβ1 in cutaneous wound healing. Using a transgenic mouse with a targeted deletion of TGFβ1 in megakaryocytes and platelets (TGFβ1fl/fl .PF4-Cre), we show for the first time that platelet-derived TGFβ1 contributes to epidermal and dermal thickening and cellular turnover after excisional skin wounding. In vitro studies demonstrate that human dermal fibroblasts stimulated with platelet lysate containing high levels of platelet-derived TGFβ1 did not exhibit enhanced collagen deposition or proliferation, suggesting that platelet-derived TGFβ1 is not a key promoter of these wound healing processes. Interestingly, human keratinocytes displayed enhanced TGFβ1-driven proliferation in response to platelet lysate, reminiscent of our in vivo findings. In summary, our novel findings define and emphasise an important role of platelet-derived TGFβ1 in epidermal remodelling and regeneration processes during cutaneous wound healing

A comprehensive approach to establish the impact of worksites air emissions

Worksite activities are time-limited events associated with continuous releases of airborne pollutants, such as carbon monoxide, particulate matter, and NOx, and they impact potentially vast areas. The side-effects on the environment can be severe, and they are subject of literature studies, with the final aim of proposing solutions that may improve the management of air emissions. No general assessment method or approach is yet available to estimate their effects on the environment and workers’ health. In this work, a general procedure that can be potentially applied to every type of worksite is proposed (i.e., construction sites, upgrading of chemical plants, road sites, etc..). The approach involves a detailed assessment of emissions and their expected pollutant concentrations. A dedicated mathematical model has been defined to assess pollutant emissions over time, consistent with all the different phases of foreseen activities. Emissions are defined on base of the GANTT descriptions of the activities and air pollutant dispersion is simulated with a dedicated model. Finally, the obtained results are evaluated against air quality thresholds as defined by laws and conditioning the human health risks for workers and citizens potentially exposed to pollutants

Duchenne muscular dystrophy: From diagnosis to therapy

Duchenne muscular dystrophy (DMD) is an X-linked inherited neuromuscular disorder due to mutations in the dystrophin gene. It is characterized by progressive muscle weakness and wasting due to the absence of dystrophin protein that causes degeneration of skeletal and cardiac muscle. The molecular diagnostic of DMD involves a deletions/duplications analysis performed by quantitative technique such as microarray-based comparative genomic hybridization (array-CGH), Multiple Ligation Probe Assay MLPA. Since traditional methods for detection of point mutations and other sequence variants require high cost and are time consuming, especially for a large gene like dystrophin, the use of next-generation sequencing (NGS) has become a useful tool available for clinical diagnosis. The dystrophin gene is large and finely regulated in terms of tissue expression, and RNA processing and editing includes a variety of fine tuned processes. At present, there are no effective treatments and the steroids are the only fully approved drugs used in DMD therapy able to slow disease progression. In the last years, an increasing variety of strategies have been studied as a possible therapeutic approach aimed to restore dystrophin production and to preserve muscle mass, ameliorating the DMD phenotype. RNA is the most studied target for the development of clinical strategies and Antisense Oligonucleotides (AONs) are the most used molecules for RNA modulation. The identification of delivery system to enhance the efficacy and to reduce the toxicity of AON is the main purpose in this area and nanomaterials are a very promising model as DNA/RNA molecules vectors. Dystrophinopathies therefore represent a pivotal field of investigation, which has opened novel avenues in molecular biology, medical genetics and novel therapeutic options

Advantages of the recursive operability analysis in updating the risk assessment

With the introduction of new regulations and sustainable technologies, revamping and upgrading already existing chemical plants is nowadays an important element in the framework of process engineering. Such important modifications must come along in parallel improvement of process safety. In this sense, risk assessment is a tool that should be versatile and easy to update by definition. However, even the most common methods currently used for accidental scenarios identification and risk assessment estimation (such as HazOp) may prove to be very time-consuming when discussing about safety from process modifications. The availability of a reliable and easy-to-update tool for safety engineering is crucial for process industries. In this work, we compare a risk analysis on a chemical plant subject of modifications performed with two different tools: HazOp and FTA vs Recursive Operability Analysis (ROA) and FTA. Both techniques have been applied to a tank dedicated to dust mixing that was subject of process modifications. Both methods come to the same conclusions, highlighting new failures and process criticalities, associated with the introduction of flow alarms and interlocks in case of excessive depressurizing. It is shown that the Recursive Operability Analysis, with its cause-consequence structure tied with process variable interactions, is much more effective in a risk assessment update

Assumptions behind grammatical approaches to code-switching: when the blueprint is a red herring

Many of the so-called ‘grammars’ of code-switching are based on various underlying assumptions, e.g. that informal speech can be adequately or appropriately described in terms of ‘‘grammar’’; that deep, rather than surface, structures are involved in code-switching; that one ‘language’ is the ‘base’ or ‘matrix’; and that constraints derived from existing data are universal and predictive. We question these assumptions on several grounds. First, ‘grammar’ is arguably distinct from the processes driving speech production. Second, the role of grammar is mediated by the variable, poly-idiolectal repertoires of bilingual speakers. Third, in many instances of CS the notion of a ‘base’ system is either irrelevant, or fails to explain the facts. Fourth, sociolinguistic factors frequently override ‘grammatical’ factors, as evidence from the same language pairs in different settings has shown. No principles proposed to date account for all the facts, and it seems unlikely that ‘grammar’, as conventionally conceived, can provide definitive answers. We conclude that rather than seeking universal, predictive grammatical rules, research on CS should focus on the variability of bilingual grammars

Biocompatibility and toxicity of graphene quantum dots for potential application in photodynamic therapy

This is the author accepted manuscript. The final version is available from Future Medicine via the DOI in this recordAim: Achieving reliably high production of reactive oxygen species (ROS) in photodynamic therapy (PDT) remains challenging. Graphene quantum dots (GQD) hold great promise for PDT. However, the photochemical processes leading to GQD-derived ROS generation have not yet been fully elucidated. Materials & methods: Physicochemical characteristics of GQDs were comprehensively investigated, including electron paramagnetic resonance analysis of singlet oxygen production. Dark toxicity was assessed in vitro and in vivo. Results: GQDs demonstrated excellent photo-luminescent features, corrosion resistance, high water solubility, high photo/pH-stability, in vitro and in vivo biocompatibility and very efficient singlet oxygen/ROS generation. Conclusion: The enhanced ROS generation, combined with good biocompatibility and minimal toxicity in vitro and in vivo support the potential of GQDs for future PDT application.This work was supported by the EPSRC Centre for Doctoral Training in Metamaterials, XM2 (grant number EP/L015331/1

The Rg1 allele as a valuable tool for genetic transformation of the tomato 'Micro-Tom' model system.

Artigo número 23