Petrology of the Ordovician Swan Peak Formation, Southeastern Idaho and North-Central Utah

The Swan Peak Formation in southeastern Idaho and north-central Utah is a sedimentary unit consisting of orthoquartzite, sandstone, siltite, shale, and limestone. The formation is divisible into three members, and the lower two members each are divisible into two informal lithologic subunits. The lower member consists of a lower subunit of gray, calcareous sandy siltite composed of subangular to subrounded quartz grains cemented by quartz overgrowths, calcite, or iron oxide, and an upper subunit of black shale with minor interbedded silty quartzose sandstone and biomicrite (limestone). The middle member consists of a lower subunit of interbedded pale green shale and yellowish brown silty orthoquartzite and an upper subunit of purple orthoquartzite. The brown orthoquartzite consists of well-sorted, well-rounded very fine sand- to silt-sized quartz grains cemented by quartz overgrowths which are in optical continuity with the grains they surround. The purple orthoquartzite consists of wellsorted, well-rounded, very fine to medium sand-sized quartz grains cemented by quartz overgrowths and hematite. Hematite gives the rock its purple color. gydroxylapatite is locally abundant. The upper member is an orthoquartzite consisting of very fine to medium sand-sized, well-sorted, well-rounded quartz grains cemented by quartz overgrowths. The gastropod Murchisonia (Hormotoma) sp., the first body fossil found in the upper member, is reported. Previous work has shown that the upper member of the Swan Peak Formation and the Eureka Quartzite are similar in lithology, stratigraphy, and trace fossils. The Eureka Quartzite in the Newfoundland Range is a very fine to medium sand-sized, well-sorted, well-rounded orthoquartzite cemented predominantly by quartz overgrowths, locally by dolomite. The petrographic similarities of the two units, shown in the present study, strengthens their proposed correlation. High percentages of well rounded, polycrystalline and undulatory extinction quartz show that source areas for the Eureka Quartzite and Swan Peak Formation probably were immature sandstones or quartzites of Cambrian or Precambrian age, and./or exposed igneous or metamorphic rocks. The source for most of the sand probably was the Northwest Montana Uplift, although local sources along the Uinta Uplift undoubtedly played a minor role in supplying hydroxylapatite to the middle member and fine-grained elastics to the lower member

Hypoinsulinemia Regulates Amphetamine-Induced Reverse Transport of Dopamine

The behavioral effects of psychomotor stimulants such as amphetamine (AMPH) arise from their ability to elicit increases in extracellular dopamine (DA). These AMPH-induced increases are achieved by DA transporter (DAT)-mediated transmitter efflux. Recently, we have shown that AMPH self-administration is reduced in rats that have been depleted of insulin with the diabetogenic agent streptozotocin (STZ). In vitro studies suggest that hypoinsulinemia may regulate the actions of AMPH by inhibiting the insulin downstream effectors phosphotidylinositol 3-kinase (PI3K) and protein kinase B (PKB, or Akt), which we have previously shown are able to fine-tune DAT cell-surface expression. Here, we demonstrate that striatal Akt function, as well as DAT cell-surface expression, are significantly reduced by STZ. In addition, our data show that the release of DA, determined by high-speed chronoamperometry (HSCA) in the striatum, in response to AMPH, is severely impaired in these insulin-deficient rats. Importantly, selective inhibition of PI3K with LY294002 within the striatum results in a profound reduction in the subsequent potential for AMPH to evoke DA efflux. Consistent with our biochemical and in vivo electrochemical data, findings from functional magnetic resonance imaging experiments reveal that the ability of AMPH to elicit positive blood oxygen level–dependent signal changes in the striatum is significantly blunted in STZ-treated rats. Finally, local infusion of insulin into the striatum of STZ-treated animals significantly recovers the ability of AMPH to stimulate DA release as measured by high-speed chronoamperometry. The present studies establish that PI3K signaling regulates the neurochemical actions of AMPH-like psychomotor stimulants. These data suggest that insulin signaling pathways may represent a novel mechanism for regulating DA transmission, one which may be targeted for the treatment of AMPH abuse and potentially other dopaminergic disorders

Soluble and Cell-Associated Insulin Receptor Dysfunction Correlates with Severity of HAND in HIV-Infected Women

Blood sugar metabolism abnormalities have been identified in HIV-infected individuals and associated with HIV-associated neurocognitive disorders (HAND). These abnormalities may occur as a result of chronic HIV infection, long-term use of combined antiretroviral treatment (CART), aging, genetic predisposition, or a combination of these factors, and may increase morbidity and mortality in this population.To determine if changes in soluble and cell-associated insulin receptor (IR) levels, IR substrate-1 (IRS-1) levels, and IRS-1 tyrosine phosphorylation are associated with the presence and severity of HAND in a cohort of HIV-seropositive women.This is a retrospective cross-sectional study using patient database information and stored samples from 34 HIV-seropositive women and 10 controls without history of diabetes from the Hispanic-Latino Longitudinal Cohort of Women. Soluble IR subunits [sIR, ectodomain (α) and full-length or intact (αβ)] were assayed in plasma and CSF samples by ELISA. Membrane IR levels, IRS-1 levels, and IRS-1 tyrosine phosphorylation were analyzed in CSF white cell pellets (WCP) using flow cytometry. HIV-seropositive women had significantly increased levels of intact or full-length sIR in plasma (p<0.001) and CSF (p<0.005) relative to controls. Stratified by HAND, increased levels of full-length sIR in plasma were associated with the presence (p<0.001) and severity (p<0.005) of HAND. A significant decrease in IRS-1 tyrosine-phosphorylation in the WCP was also associated with the presence (p<0.02) and severity (p<0.02) of HAND.This study provides evidence that IR secretion is increased in HIV-seropositive women, and increased IR secretion is associated with cognitive impairment in these women. Thus, IR dysfunction may have a role in the progression of HAND and could represent a biomarker for the presence and severity of HAND