Klassen- und Schulsprecherqualifizierung als zentrales Element einer demokratischen Schulkultur. Gesamtschule Süderelbe, Hamburg

An der Gesamtschule Süderelbe in Hamburg wurde zwischen 2003 und 2006 ein Konzept zur Qualifizierung von Klassen- und Schulsprechern entwickelt. Die Schüler/-innen lernen, ihre Partizipation in den Gremien systematisch zu planen, ihre Bedürfnisse wirksam zu artikulieren und ihre Interessen nach demokratischen Regeln durchzusetzen. Dieses Konzept wird zunehmend verbunden mit weiteren Teilen demokratischer Schulkultur – Klassenrat, Streitschlichtung und Jugendgruppenleiterausbildung

Reprocessed precise science orbits and gravity field recovery for the entire GOCE mission.

ESA’s Gravity field and steady-state Ocean Circulation Explorer (GOCE) orbited the Earth between 2009 and 2013 for the determination of the static part of Earth’s gravity field. The GPS-derived precise science orbits (PSOs) were operationally generated by the Astronomical Institute of the University of Bern (AIUB). Due to a significantly improved understanding of remaining artifacts after the end of the GOCE mission (especially in the GOCE gradiometry data), ESA initiated a reprocessing of the entire GOCE Level 1b data in 2018. In this framework, AIUB was commissioned to recompute the GOCE reduced-dynamic and kinematic PSOs. In this paper, we report on the employed precise orbit determination methods, with a focus on measures undertaken to mitigate ionosphere-induced artifacts in the kinematic orbits and thereof derived gravity field models. With respect to the PSOs computed during the operational phase of GOCE, the reprocessed PSOs show in average a 8–9% better consistency with GPS data, 31% smaller 3-dimensional reduced-dynamic orbit overlaps, an 8% better 3-dimensional consistency between reduced-dynamic and kinematic orbits, and a 3–7% reduction of satellite laser ranging residuals. In the second part of the paper, we present results from GPS-based gravity field determinations that highlight the strong benefit of the GOCE reprocessed kinematic PSOs. Due to the applied data weighting strategy, a substantially improved quality of gravity field coefficients between degree 10 and 40 is achieved, corresponding to a remarkable reduction of ionosphere-induced artifacts along the geomagnetic equator. For a static gravity field solution covering the entire mission period, geoid height differences with respect to a superior inter-satellite ranging solution are markedly reduced (43% in terms of global RMS, compared to previous GOCE GPS-based gravity fields). Furthermore, we demonstrate that the reprocessed GOCE PSOs allow to recover long-wavelength time-variable gravity field signals (up to degree 10), comparable to information derived from GPS data of dedicated satellite missions. To this end, it is essential to take into account the GOCE common-mode accelerometer data in the gravity field recovery

Reprocessed precise science orbits and gravity field recovery for the entire GOCE mission

ESA’s Gravity field and steady-state Ocean Circulation Explorer (GOCE) orbited the Earth between 2009 and 2013 for the determination of the static part of Earth’s gravity field. The GPS-derived precise science orbits (PSOs) were operationally generated by the Astronomical Institute of the University of Bern (AIUB). Due to a significantly improved understanding of remaining artifacts after the end of the GOCE mission (especially in the GOCE gradiometry data), ESA initiated a reprocessing of the entire GOCE Level 1b data in 2018. In this framework, AIUB was commissioned to recompute the GOCE reduced-dynamic and kinematic PSOs. In this paper, we report on the employed precise orbit determination methods, with a focus on measures undertaken to mitigate ionosphere-induced artifacts in the kinematic orbits and thereof derived gravity field models. With respect to the PSOs computed during the operational phase of GOCE, the reprocessed PSOs show in average a 8–9% better consistency with GPS data, 31% smaller 3-dimensional reduced-dynamic orbit overlaps, an 8% better 3-dimensional consistency between reduced-dynamic and kinematic orbits, and a 3–7% reduction of satellite laser ranging residuals. In the second part of the paper, we present results from GPS-based gravity field determinations that highlight the strong benefit of the GOCE reprocessed kinematic PSOs. Due to the applied data weighting strategy, a substantially improved quality of gravity field coefficients between degree 10 and 40 is achieved, corresponding to a remarkable reduction of ionosphere-induced artifacts along the geomagnetic equator. For a static gravity field solution covering the entire mission period, geoid height differences with respect to a superior inter-satellite ranging solution are markedly reduced (43% in terms of global RMS, compared to previous GOCE GPS-based gravity fields). Furthermore, we demonstrate that the reprocessed GOCE PSOs allow to recover long-wavelength time-variable gravity field signals (up to degree 10), comparable to information derived from GPS data of dedicated satellite missions. To this end, it is essential to take into account the GOCE common-mode accelerometer data in the gravity field recovery

Better than expected: the gap between self-reported and objective measures of cognitive performance in remitted bipolar disorder

Background: Studies comparing objective and self-reported cognitive functioning as well as influencing factors in individuals with remitted bipolar disorder are scarce and contradictory. Methods: The aim of this study was to compare executive functioning and other objective and self-reported cognitive impairment between 26 individuals with remitted bipolar disorder (15 BD I) and 24 healthy controls using a cross-sectional design. Executive functions were measured by the TAP Go/No-go subtest as well as the Stroop Task. Self-rated functioning was assessed using the Attention Deficit Experience Questionnaire. In addition, possible predictors of self-reported and objective cognitive functioning were examined to perform regression analyses. Results: Individuals with remitted bipolar disorder did not differ significantly in executive functions or other objective cognitive domains from the healthy control group, but showed a significantly lower level of self-reported cognitive functioning and self-esteem. While self-esteem was the strongest predictor in healthy controls for self-reported cognitive functioning, severity of illness and subthreshold depressive mood were the most important predictors in individuals with remitted bipolar disorder. Conclusion: The results once again demonstrate the cognitive heterogeneity in bipolar disorder. In the treatment of cognitive deficits, factors such as subthreshold depressive symptomatology and self-esteem should be focused on in addition to cognitive training in remitted patients

Better than expected: the gap between self-reported and objective measures of cognitive performance in remitted bipolar disorder

Background: Studies comparing objective and self-reported cognitive functioning as well as influencing factors in individuals with remitted bipolar disorder are scarce and contradictory. Methods: The aim of this study was to compare executive functioning and other objective and self-reported cognitive impairment between 26 individuals with remitted bipolar disorder (15 BD I) and 24 healthy controls using a cross-sectional design. Executive functions were measured by the TAP Go/No-go subtest as well as the Stroop Task. Self-rated functioning was assessed using the Attention Deficit Experience Questionnaire. In addition, possible predictors of self-reported and objective cognitive functioning were examined to perform regression analyses. Results: Individuals with remitted bipolar disorder did not differ significantly in executive functions or other objective cognitive domains from the healthy control group, but showed a significantly lower level of self-reported cognitive functioning and self-esteem. While self-esteem was the strongest predictor in healthy controls for self-reported cognitive functioning, severity of illness and subthreshold depressive mood were the most important predictors in individuals with remitted bipolar disorder. Conclusion: The results once again demonstrate the cognitive heterogeneity in bipolar disorder. In the treatment of cognitive deficits, factors such as subthreshold depressive symptomatology and self-esteem should be focused on in addition to cognitive training in remitted patients

Better than expected: the gap between self-reported and objective measures of cognitive performance in remitted bipolar disorder

BackgroundStudies comparing objective and self-reported cognitive functioning as well as influencing factors in individuals with remitted bipolar disorder are scarce and contradictory.MethodsThe aim of this study was to compare executive functioning and other objective and self-reported cognitive impairment between 26 individuals with remitted bipolar disorder (15 BD I) and 24 healthy controls using a cross-sectional design. Executive functions were measured by the TAP Go/No-go subtest as well as the Stroop Task. Self-rated functioning was assessed using the Attention Deficit Experience Questionnaire. In addition, possible predictors of self-reported and objective cognitive functioning were examined to perform regression analyses.ResultsIndividuals with remitted bipolar disorder did not differ significantly in executive functions or other objective cognitive domains from the healthy control group, but showed a significantly lower level of self-reported cognitive functioning and self-esteem. While self-esteem was the strongest predictor in healthy controls for self-reported cognitive functioning, severity of illness and subthreshold depressive mood were the most important predictors in individuals with remitted bipolar disorder.ConclusionThe results once again demonstrate the cognitive heterogeneity in bipolar disorder. In the treatment of cognitive deficits, factors such as subthreshold depressive symptomatology and self-esteem should be focused on in addition to cognitive training in remitted patients

MRI-TRUS fusion for electrode positioning during irreversible electroporation for treatment of prostate cancer

We aimed to introduce an approach for image-guided positioning of electrodes for irreversible electroporation (IRE) in patients with prostate cancer using a magnetic resonance imaging-transrectal ultrasonography (MRI-TRUS) fusion technique. In 10 consecutive patients with biopsy-proven Gleason score ≤3+4 prostate cancer, 19 G electrodes were inserted into the prostate using a transperineal access. Magnetic resonance images of the prostate acquired before IRE were fused with transrectal ultrasound images acquired during IRE. The position of the ultrasound probe was tracked via a sensor and corresponding magnetic resonance images were calculated in real-time. While MRI allowed delineation of the target volume, the position of the electrodes could be visualized on ultrasound images; the distance between individual electrode pairs was measured. Based on these measurements the software installed on the IRE unit was able to calculate the voltage necessary to generate the electric field for ablation. Using contrast-enhanced ultrasound, changes in perfusion within the ablation zone after IRE were documented. This technique allowed positioning of the electrodes around the target volume under image guidance in all patients treated with IRE. The target lesion and a safety margin were covered within the estimated ablation zone. MRI-TRUS guidance for IRE combines the advantages of good visualization of the target lesion on MRI with the ability of ultrasound to acquire imaging in real-time with a mobile device

The Transcription Factor AmrZ Utilizes Multiple DNA Binding Modes to Recognize Activator and Repressor Sequences of Pseudomonas aeruginosa Virulence Genes

AmrZ, a member of the Ribbon-Helix-Helix family of DNA binding proteins, functions as both a transcriptional activator and repressor of multiple genes encoding Pseudomonas aeruginosa virulence factors. The expression of these virulence factors leads to chronic and sustained infections associated with worsening prognosis. In this study, we present the X-ray crystal structure of AmrZ in complex with DNA containing the repressor site, amrZ1. Binding of AmrZ to this site leads to auto-repression. AmrZ binds this DNA sequence as a dimer-of-dimers, and makes specific base contacts to two half sites, separated by a five base pair linker region. Analysis of the linker region shows a narrowing of the minor groove, causing significant distortions. AmrZ binding assays utilizing sequences containing variations in this linker region reveals that secondary structure of the DNA, conferred by the sequence of this region, is an important determinant in binding affinity. The results from these experiments allow for the creation of a model where both intrinsic structure of the DNA and specific nucleotide recognition are absolutely necessary for binding of the protein. We also examined AmrZ binding to the algD promoter, which results in activation of the alginate exopolysaccharide biosynthetic operon, and found the protein utilizes different interactions with this site. Finally, we tested the in vivo effects of this differential binding by switching the AmrZ binding site at algD, where it acts as an activator, for a repressor binding sequence and show that differences in binding alone do not affect transcriptional regulation

Common Genetic Variation at the IL1RL1 Locus Regulates IL-33/ST2 Signaling

The suppression of tumorigenicity 2/IL-33 (ST2/IL-33) pathway has been implicated in several immune and inflammatory diseases. ST2 is produced as 2 isoforms. The membrane-bound isoform (ST2L) induces an immune response when bound to its ligand, IL-33. The other isoform is a soluble protein (sST2) that is thought to be a decoy receptor for IL-33 signaling. Elevated sST2 levels in serum are associated with an increased risk for cardiovascular disease. We investigated the determinants of sST2 plasma concentrations in 2,991 Framingham Offspring Cohort participants. While clinical and environmental factors explained some variation in sST2 levels, much of the variation in sST2 production was driven by genetic factors. In a genome-wide association study (GWAS), multiple SNPs within IL1RL1 (the gene encoding ST2) demonstrated associations with sST2 concentrations. Five missense variants of IL1RL1 correlated with higher sST2 levels in the GWAS and mapped to the intracellular domain of ST2, which is absent in sST2. In a cell culture model, IL1RL1 missense variants increased sST2 expression by inducing IL-33 expression and enhancing IL-33 responsiveness (via ST2L). Our data suggest that genetic variation in IL1RL1 can result in increased levels of sST2 and alter immune and inflammatory signaling through the ST2/IL-33 pathway.Stem Cell and Regenerative Biolog