Cytokines of Birds: Conserved Functions

Targeted disruptions of the mouse genes for cytokines, cytokine receptors, or components of cytokine signaling cascades convincingly revealed the important roles of these molecules in immunologic processes. Cytokines are used at present as drugs to fight chronic microbial infections and cancer in humans, and they are being evaluated as immune response modifiers to improve vaccines. Until recently, only a few avian cytokines have been characterized, and potential applications thus have remained limited to mammals. Classic approaches to identify cytokine genes in birds proved difficult because sequence conservation is generally low. As new technology and high throughput sequencing became available, this situation changed quickly. We review here recent work that led to the identification of genes for the avian homologs of interferon-a/b (IFNa/b) and IFN-g, various interleukins (IL), and several chemokines. From the initial data on the biochemical properties of these molecules, a picture is emerging that shows that avian and mammalian cytokines may perform similar tasks, although their primary structures in most cases are remarkably different

Intake of silica nanoparticles by giant lipid vesicles: influence of particle size and thermodynamic membrane state

The uptake of nanoparticles into cells often involves their engulfment by the plasma membrane and a fission of the latter. Understanding the physical mechanisms underlying these uptake processes may be achieved by the investigation of simple model systems that can be compared to theoretical models. Here, we present experiments on a massive uptake of silica nanoparticles by giant unilamellar lipid vesicles (GUVs). We find that this uptake process depends on the size of the particles as well as on the thermodynamic state of the lipid membrane. Our findings are discussed in the light of several theoretical models and indicate that these models have to be extended in order to capture the interaction between nanomaterials and biological membranes correctly

Intake of silica nanoparticles by giant lipid vesicles: influence of particle size and thermodynamic membrane state

The uptake of nanoparticles into cells often involves their engulfment by the plasma membrane and a fission of the latter. Understanding the physical mechanisms underlying these uptake processes may be achieved by the investigation of simple model systems that can be compared to theoretical models. Here, we present experiments on a massive uptake of silica nanoparticles by giant unilamellar lipid vesicles (GUVs). We find that this uptake process depends on the size of the particles as well as on the thermodynamic state of the lipid membrane. Our findings are discussed in the light of several theoretical models and indicate that these models have to be extended in order to capture the interaction between nanomaterials and biological membranes correctly

A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes

Static conditions represent an important shortcoming of many in vitro experiments on the cellular uptake of nanoparticles. Here, we present a versatile microfluidic device based on acoustic streaming induced by surface acoustic waves (SAWs). The device offers a convenient method for introducing fluid motion in standard cell culture chambers and for mimicking capillary blood flow. We show that shear rates over the whole physiological range in sample volumes as small as 200 mu L can be achieved. A precise characterization method for the induced flow profile is presented and the influence of flow on the uptake of Pt-decorated CeO2 particles by endothelial cells (HMEC-1) is demonstrated. Under physiological flow conditions the particle uptake rates for this system are significantly lower than at low shear conditions. This underlines the vital importance of the fluidic environment for cellular uptake mechanisms

Flux Periodicities in Loops of Nodal Superconductors

Supercurrents in superconducting flux threaded loops are expected to oscillate with the magnetic flux with a period of hc/2e. This is indeed true for s-wave superconductors larger than the coherence length xi_0. Here we show that for superconductors with gap nodes, there is no such strict condition for the supercurrent to be hc/2e rather than hc/e periodic. For nodal superconductors, the flux induced Doppler shift of the near nodal states leads to a flux dependent occupation probability of quasi-particles circulating clockwise and counter clockwise around the loop, which leads to an hc/e periodic component of the supercurrent, even at zero temperature. We analyze this phenomenon on a cylinder in an approximative analytic approach and also numerically within the framework of the BCS theory. Specifically for d-wave pairing, we show that the hc/e periodic current component decreases with the inverse radius of the loop and investigate its temperature dependence

Late Quaternary aeolian dynamics, pedostratigraphy and soil formation in the North European Lowlands – new findings from the Baruther ice-marginal valley

The construction of dunes in central Europe reflects ample sediment supply during the last deglacial hemicycle. A Quaternary inland dune complex in southern Brandenburg, Germany, was studied to determine the duration of recent pedogenesis, from two outcrops, which show buried paleosols. An integrative approach, which combined geomorphological, sedimentological, (paleo-)pedological and chronological methods was used to identify aeolian deposition events, ensuing pedogenesis and anthropogenic remobilization. At the outcrops, which were situated approximately 2 km apart from each other, in total twelve samples of the aeolian sands were dated using optically stimulated luminescence (OSL) and six using 14C dating. Although the dunes have similar morphological features, these forms have a different history of aeolian sand deposition and pedogenesis. At the older dune (Gl 1) the surface soil is a well developed Podzol, whereas soil development of the younger dune (Gl 2) is clearly in an initial state. The two dunes also differ in grain size distribution and in the presence of buried soils, thereby indicating a climatic impact on aeolian remobilization

The classification of irreducible admissible mod p representations of a p-adic GL_n

Let F be a finite extension of Q_p. Using the mod p Satake transform, we define what it means for an irreducible admissible smooth representation of an F-split p-adic reductive group over \bar F_p to be supersingular. We then give the classification of irreducible admissible smooth GL_n(F)-representations over \bar F_p in terms of supersingular representations. As a consequence we deduce that supersingular is the same as supercuspidal. These results generalise the work of Barthel-Livne for n = 2. For general split reductive groups we obtain similar results under stronger hypotheses.Comment: 55 pages, to appear in Inventiones Mathematica

Lorentz angle measurements in irradiated silicon detectors between 77 K and 300 K

Future experiments are using silicon detectors in a high radiation environment and in high magnetic fields. The radiation tolerance of silicon improves by cooling it to temperatures below 180 K. At low temperatures the mobility increases, which leads to larger de of the charge carriers by the Lorentz force. A good knowledge of the Lorentz angle is needed for design and operation of silicon detectors. We present measurements of the Lorentz angle between 77 K and 300 K before and after irradiation with a primary beam of 21 MeV protons

Automatically proving termination and memory safety for programs with pointer arithmetic

While automated verification of imperative programs has been studied intensively, proving termination of programs with explicit pointer arithmetic fully automatically was still an open problem. To close this gap, we introduce a novel abstract domain that can track allocated memory in detail. We use it to automatically construct a symbolic execution graph that over-approximates all possible runs of a program and that can be used to prove memory safety. This graph is then transformed into an integer transition system, whose termination can be proved by standard techniques. We implemented this approach in the automated termination prover AProVE and demonstrate its capability of analyzing C programs with pointer arithmetic that existing tools cannot handle

Ground-based detection of an extended helium atmosphere in the Saturn-mass exoplanet WASP-69b

Hot gas giant exoplanets can lose part of their atmosphere due to strong stellar irradiation, affecting their physical and chemical evolution. Studies of atmospheric escape from exoplanets have mostly relied on space-based observations of the hydrogen Lyman-{\alpha} line in the far ultraviolet which is strongly affected by interstellar absorption. Using ground-based high-resolution spectroscopy we detect excess absorption in the helium triplet at 1083 nm during the transit of the Saturn-mass exoplanet WASP-69b, at a signal-to-noise ratio of 18. We measure line blue shifts of several km/s and post transit absorption, which we interpret as the escape of part of the atmosphere trailing behind the planet in comet-like form. [Additional notes by authors: Furthermore, we provide upper limits for helium signals in the atmospheres of the exoplanets HD 209458b, KELT-9b, and GJ 436b. We investigate the host stars of all planets with detected helium signals and those of the three planets we derive upper limits for. In each case we calculate the X-ray and extreme ultraviolet flux received by these planets. We find that helium is detected in the atmospheres of planets (orbiting the more active stars and) receiving the larger amount of irradiation from their host stars.]Comment: Submitted to Science on 14 March 2018; Accepted by Science on 16 November 2018; Published by Science on 6 December 2018. This is the author's version of the work. It is posted here by permission of the AAAS for personal use. The definitive version was published in Science, on 6 December 2018 - Report: pages 21 (preprint), 4 figures - Supplementary materials: 22 pages, 10 figures, 3 table