The MobyDick Project: A Mobile Heterogeneous All-IP Architecture

Proceedings of Advanced Technologies, Applications and Market Strategies for 3G (ATAMS 2001). Cracow, Poland: 17-20 June, 2001.This paper presents the current stage of an IP-based architecture for heterogeneous environments, covering UMTS-like W-CDMA wireless access technology, wireless and wired LANs, that is being developed under the aegis of the IST Moby Dick project. This architecture treats all transmission capabilities as basic physical and data-link layers, and attempts to replace all higher-level tasks by IP-based strategies. The proposed architecture incorporates aspects of mobile-IPv6, fast handover, AAA-control, and Quality of Service. The architecture allows for an optimised control on the radio link layer resources. The Moby dick architecture is currently under refinement for implementation on field trials. The services planned for trials are data transfer and voice-over-IP.Publicad

Modelling personality features by changing prosody in synthetic speech

This study explores how features of brand personalities can be modelled with the prosodic parameters pitch level, pitch range, articulation rate and loudness. Experiments with parametrical diphone synthesis showed that listeners rated the prosodically changed versions better than a baseline version for the dimension

Modelling personality features by changing prosody in synthetic speech

This study explores how features of brand personalities can be modelled with the prosodic parameters pitch level, pitch range, articulation rate and loudness. Experiments with parametrical diphone synthesis showed that listeners rated the prosodically changed versions better than a baseline version for the dimension

Molecular structure of a novel cholesterol-responsive A subclass ABC transporter, ABCA9

We recently identified a novel ABC A subclass transporter, ABCA6, in human macrophages. Here, we report the molecular cloning of an additional ABC A subfamily transporter from macrophages denoted ABCA9. The identified coding sequence is 4.9 kb in size and codes for a 1624 amino acid protein product. In accordance with the proposed nomenclature, the novel transporter was designated ABCA9. The putative full-length ABC transporter polypeptide consists of two transmembrane domains and two nucleotide binding folds and thus conforms to the group of full-size ABC transporters. We identified alternative ABCA9 mRNA variants in human macrophages that predict the existence of three truncated forms of the novel transporter. Among the human ABC A subfamily transporters, ABCA9 exhibits the highest amino acid sequence homology with ABCA8 (72%) and ABCA6 (60%), respectively. The striking amino acid sequence similarity between these transporter molecules supports the notion that they represent an evolutionary more recently emerged subgroup within the family of ABC A transporters, which we refer to as “ABCA6-like transporters.” ABCA9 mRNA is ubiquitously expressed with the highest mRNA levels in heart, brain, and fetal tissues. Analysis of the genomic structure revealed that the ABCA9 gene consists of 39 exons that are located within a genomic region of ∼85 kb size on chromosome 17q24.2. In human macrophages, ABCA9 mRNA is induced during monocyte differentiation into macrophages and suppressed by cholesterol import indicating that ABCA9, like other known ABC A subfamily transporters, is a cholesterol-responsive gene. Based on this information, ABCA9 is likely involved in monocyte differentiation and macrophage lipid homeostasis

Monophyly of social wasps

20 p. : ill. ; 26 cm.Includes bibliographical references (p. 12-13).The alignment of molecular sequence data published by Schmitz and Moritz (1998. Molecular phylogeny of Vespidae (Hymenoptera) and the evolution of sociality in wasps, Molecular Phylogenetics and Evolution 9: 183-191) supported closer phylogenetic relationship of Eumeninae to Polistinae + Vespinae than Stenogastrinae, from which they concluded that social behavior has independently evolved twice in the wasp family Vespidae. However, their analyses also showed the Vespidae as paraphyletic in terms of the bee family Apidae. Simultaneous analysis of these molecular data with published morphological and behavioral characters is presented. The resulting cladograms support monophyly of Vespidae, as well as monophyly of social wasps, with the primitively social Stenogastrinae being more closely related to the highly social Polistinae + Vespinae than the solitary Eumeninae. A realignment of the sequence data is also presented, which is more parsimonious than that published by Schmitz and Moritz. Analysis of the realigned sequences also supports monophyly of Vespidae, as well as monophyly of social wasps, with the Stenogastrinae being more closely related to Polistinae + Vespinae than are Eumeninae

Protein phosphatase 2A affects myofilament contractility in non-failing but not in failing human myocardium

Protein phosphatase (PP) type 2A is a multifunctional serine/threonine phosphatase that is involved in cardiac excitation–contraction coupling. The PP2A core enzyme is a dimer, consisting of a catalytic C and a scaffolding A subunit, which is targeted to several cardiac proteins by a regulatory B subunit. At present, it is controversial whether PP2A and its subunits play a critical role in end-stage human heart failure. Here we report that the application of purified PP2AC significantly increased the Ca2+-sensitivity (ΔpCa50 = 0.05 ± 0.01) of the contractile apparatus in isolated skinned myocytes of non-failing (NF) hearts. A higher phosphorylation of troponin I (cTnI) was found at protein kinase A sites (Ser23/24) in NF compared to failing myocardium. The basal Ca2+-responsiveness of myofilaments was enhanced in myocytes of ischemic (ICM, ΔpCa50 = 0.10 ± 0.03) and dilated (DCM, ΔpCa50 = 0.06 ± 0.04) cardiomyopathy compared to NF. However, in contrast to NF myocytes the treatment with PP2AC did not shift force-pCa relationships in failing myocytes. The higher basal Ca2+-sensitivity in failing myocytes coincided with a reduced protein expression of PP2AC in left ventricular tissue from patients suffering from ICM and DCM (by 50 and 56% compared to NF, respectively). However, PP2A activity was unchanged in failing hearts despite an increase of both total PP and PP1 activity. The expression of PP2AB56α was also decreased by 51 and 62% in ICM and DCM compared to NF, respectively. The phosphorylation of cTnI at Ser23/24 was reduced by 66 and 49% in ICM and DCM compared to NF hearts, respectively. Our results demonstrate that PP2A increases myofilament Ca2+-sensitivity in NF human hearts, most likely via cTnI dephosphorylation. This effect is not present in failing hearts, probably due to the lower baseline cTnI phosphorylation in failing compared to non-failing hearts

Deep water flow in the Mariana and Caroline Basins

Two major water masses dominate the deep layers in the Mariana and Caroline Basins: the Lower Circumpolar Water (LCPW), arriving from the Southern Ocean along the slopes north of the Marshall Islands, and the North Pacific Deep Water (NPDW) reaching the region from the northeastern Pacific Ocean. Hydrographic and moored observations and multibeam echosounding were performed in the East Mariana and the East Caroline Basins to detail watermass distributions and flow paths in the area. The LCPW enters the East Mariana Basin from the east. At about 13°N, however, in the southern part of the basin, a part of this water mass arrives in a southward western boundary flow along the Izu–Ogasawara–Mariana Ridge. Both hydrographic observations and moored current measurements lead to the conclusion that this water not only continues westward to the West Mariana Basin as suggested before, but also provides bottom water to the East Caroline Basin. The critical throughflow regions were identified by multibeam echosounding at the Yap Mariana Junction between the East and West Mariana Basins and at the Caroline Ridge between the East Mariana and East Caroline Basins. The throughflow is steady between the East and West Mariana Basins, whereas more variability is found at the Caroline Ridge. At both locations, throughflow fluctuations are correlated with watermass property variations suggesting layer-thickness changes. The total transport to the two neighboring basins is only about 1 Sverdrup (1Sv ≡ 106 m3 s−1) but has considerable impact on the watermass structure in these basins. Estimates are given for the diapycnal mixing that is required to balance the inflow into the East Caroline Basin. Farther above in the water column, the high-silica tongue of NPDW extends from the east to the far southwestern corner of the East Mariana Basin, with transports being mostly southward across the basin

Genomic analysis reveals hidden biodiversity within colugos, the sister group to primates

Colugos are among the most poorly studied mammals despite their centrality to resolving supraordinal primate relationships. Two described species of these gliding mammals are the sole living members of the order Dermoptera, distributed throughout Southeast Asia. We generated a draft genome sequence for a Sunda colugo and a Philippine colugo reference alignment, and used these to identify colugo-specific genetic changes that were enriched in sensory and musculoskeletal-related genes that likely underlie their nocturnal and gliding adaptations. Phylogenomic analysis and catalogs of rare genomic changes overwhelmingly support the contested hypothesis that colugos are the sister group to primates (Primatomorpha), to the exclusion of treeshrews. We captured ~140 kb of orthologous sequence data from colugo museum specimens sampled across their range and identified large genetic differences between many geographically isolated populations that may result in a >300% increase in the number of recognized colugo species. Our results identify conservation units to mitigate future losses of this enigmatic mammalian order.Victor C. Mason, Gang Li Patrick Minx, Jürgen Schmitz, Gennady Churakov, Liliya Doronina, Amanda D. Melin ... et al

A randomized controlled trial to investigate the influence of low dose radiotherapy on immune stimulatory effects in liver metastases of colorectal cancer

<p>Abstract</p> <p>Background</p> <p>Insufficient migration and activation of tumor specific effector T cells in the tumor is one of the main reasons for inadequate host anti-tumor immune response. External radiation seems to induce inflammation and activate the immune response. This phase I/II clinical trial aims to evaluate whether low dose single fraction radiotherapy can improve T cell associated antitumor immune response in patients with colorectal liver metastases.</p> <p>Methods/Design</p> <p>This is an investigator-initiated, prospective randomised, 4-armed, controlled Phase I/II trial. Patients undergoing elective hepatic resection due to colorectal cancer liver metastasis will be enrolled in the study. Patients will receive 0 Gy, 0.5 Gy, 2 Gy or 5 Gy radiation targeted to their liver metastasis. Radiation will be applied by external beam radiotherapy using a 6 MV linear accelerator (Linac) with intensity modulated radiotherapy (IMRT) technique two days prior to surgical resection. All patients admitted to the Department of General-, Visceral-, and Transplantion Surgery, University of Heidelberg for elective hepatic resection are consecutively screened for eligibility into this trial, and written informed consent is obtained before inclusion. The primary objective is to assess the effect of active local external beam radiation dose on, tumor infiltrating T cells as a surrogate parameter for antitumor activity. Secondary objectives include radiogenic treatment toxicity, postoperative morbidity and mortality, local tumor control and recurrence patterns, survival and quality of life. Furthermore, frequencies of systemic tumor reactive T cells in blood and bone marrow will be correlated with clinical outcome.</p> <p>Discussion</p> <p>This is a randomized controlled patient blinded trial to assess the safety and efficiency of low dose radiotherapy on metastasis infiltrating T cells and thus potentially enhance the antitumor immune response.</p> <p>Trial registration</p> <p>ClinicalTrials.gov: <a href="http://www.clinicaltrials.gov/ct2/show/NCT01191632">NCT01191632</a></p

Tracking Marsupial Evolution Using Archaic Genomic Retroposon Insertions

Genome-wide comparisons of shared retroposon insertion patterns resolve the phylogeny of marsupials, clearly distinguishing South American and Australian species and lending support to Didelphimorphia as the basal split