Induction of reactive oxygen species and cell survival in the presence of advanced glycation end products and similar structures

AbstractAdvanced glycation end products (AGEs) that arise from the reaction of sugars with protein side chains and the terminal amino group are supposed to be involved in the pathogenesis of several diseases and therefore the effects of AGEs on cells are the objective of numerous investigations. The effects of AGEs on cells are commonly assumed to be transduced via the receptor for AGEs (RAGE) but there are also other receptors known to interact with AGEs and they are likely to be involved in signal transduction. The primary cellular effect of AGEs on cultured cells was found to be the formation of reactive oxygen species (ROS). For the present study one murine and three human cell lines were used. The effects of a set of different highly modified AGEs and AGE-like compounds derived from the incubation of different modifiers with BSA were tested for their effects on these cells. Almost all AGEs tested induced the production of reactive oxygen species (ROS) in the different cell lines although the intensity of the detected signals varied considerably between the cell lines and are strongly dependent on the AGE used for cell activation. The most highly modified BSA-species were shown to inhibit cell growth in all cell lines, whereas a moderately modified glucose derived BSA-AGE and BSA-GAred did not show any inhibitory effect on cell growth even when a high ROS formation was detected

Ultrasonic Attenuation in Clean d-Wave Superconductors

We calculate the low temperature longitudinal ultrasonic attenuation rate $\alpha_S$ in clean d-wave superconductors. We consider the contribution of previously ignored processes involving the excitation of a pair of quasi-holes or quasi-particles. These processes, which are forbidden by energy conservation in conventional s-wave superconductors, have a finite phase space in d-wave superconductors due to the presence of nodes in the gap which give rise to soft low-energy electronic excitations. We find the contribution to $\alpha_S$ from these processes to be proportional to $T$ in the regime $k_B T\ll Qv_{\Delta} \ll \Delta_0$,(ultra-low temperature regime) and to be proportional to 1/T in the region $Qv_F \ll k_BT \ll \Delta_0$, (low temperature regime) where ${\bf Q}$ is the ultrasound wave-vector and $\Delta_0$ is the maximum gap amplitude. We explicitly evaluate these terms, for parameters appropriate to the cuprates, for ${\bf Q}$ along the nodal and the antinodal directions and compare it with the contribution from processes considered earlier(I.Vekhter et al {\it Phys. Rev.}{\bf B59}, 7123(1999)). In the ultra-low temperature regime, the processes considered by us make a contribution which is smaller by about a factor of 10 for ${\bf Q}$ along the nodal direction, while along the antinodal direction it is larger by a factor of 100 or so. In the low temperature regime on the other hand the contribution made by these terms is small. However taken together with the original terms we describe a possible way to evaluate the parameter $v_F/v_\Delta$.Comment: 9 pages, RevTex, accepted for publication in Physica

Scattering by impurity-induced order parameter ``holes'' in d-wave superconductors

Nonmagnetic impurities in d-wave superconductors cause strong local suppressions of the order parameter. We investigate the observable effects of the scatterigng off such suppressions in bulk samples by treating the order parameter "hole" as a pointlike off-diagonal scatterer treated within a self-consistent t-matrix approximation. Strong scattering potentials lead to a finite-energy spectral feature in the d-wave "impurity band", the observable effects of which include enhanced low-temperature microwave power absorption and a stronger sensitivity of the London penetration depth to disorder than found previously in simpler ``dirty'' d-wave models.Comment: 5 pp. Revtex including 4 postscript figures, submitted to Phys. Rev.

Effect of magnetic frustration on single-hole spectral function in the t-t'-t''-J model

We examine the effect of the magnetic frustration J' on the single-hole spectral function in the t-t'-t''-J model. At zero temperature, the exact diagonalization (ED) and the self-consistent Born approximation (SCBA) methods are used. We find that the frustration suppresses the quasiparticle (QP) weight at small momentum k, whereas the QP peak at k=(pi/2,pi/2) remains sharp. We also show the temperature dependence of the single-hole spectral function by using the ED method. It is found that the lineshapes at (pi/2,0) and (pi/2,pi/2) show different temperature dependence. These findings are consistent with the angle-resolved photoemission data on Sr2CuO2Cl2, and indicate the importance of the magnetic frustration on the electronic states of the insulating cuprates.Comment: 5 pages, 3 EPS figures, REVTeX, To be published in Phys. Rev. B, Vol. 59, Num. 3 (15 Jan. 1999

d-Wave Model for Microwave Response of High-Tc Superconductors

We develop a simple theory of the electromagnetic response of a d- wave superconductor in the presence of potential scatterers of arbitrary s-wave scattering strength and inelastic scattering by antiferromagnetic spin fluctuations. In the clean London limit, the conductivity of such a system may be expressed in "Drude" form, in terms of a frequency-averaged relaxation time. We compare predictions of the theory with recent data on YBCO and BSSCO crystals and on YBCO films. While fits to penetration depth measurements are promising, the low temperature behavior of the measured microwave conductivity appears to be in disagreement with our results. We discuss implications for d-wave pairing scenarios in the cuprate superconductors.Comment: 33 pages, plain TeX including all macros. 16 uuencoded, compressed postscript figures are appended at the en

Coherent Potential Approximation for `d - wave' Superconductivity in Disordered Systems

A Coherent Potential Approximation is developed for s-wave and d-wave superconductivity in disordered systems. We show that the CPA formalism reproduces the standard pair-breaking formula, the self-consistent Born Approximation and the self-consistent T-matrix approximation in the appropriate limits. We implement the theory and compute T_c for s-wave and d-wave pairing using an attractive nearest neighbor Hubbard model featuring both binary alloy disorder and a uniform distribution of scattering site potentials. We determine the density of states and examine its consequences for low temperature heat capacity. We find that our results are in qualitative agreement with measurements on Zn doped YBCO superconductors.Comment: 35 pages, 23 figures, submitted to Phys Rev.

Characterization of advanced glycation end products for biochemical studies: side chain modifications and fluorescence characteristics

Advanced glycation end products (AGEs) are known to be involved in the pathogenesis of several diseases and therefore effects of AGEs on cells are the objective of numerous investigations. Since AGEs used in biochemical studies are usually not chemically characterized, comparison of data is difficult if not impossible. To find a suitable characterization protocol, human serum albumin was reacted with different concentrations of glucose, methyl glyoxal, and glyoxylic acid. The obtained AGEs were characterized with respect to the extent of side chain modifications (lysine and arginine), the carboxymethyl lysine and carbonyl content, and the fibrillar state. Additionally, their fluorescence and absorbance characteristics were extensively studied. Although we found significant differences in the degree of modification and in AGE-specific fluorescence when using different modifiers, the results provide important information and allow comparing AGEs derived from different modifier concentrations. The results also suggest strong conformational changes within the modified proteins. In the present paper we propose a set of parameters that is sufficient to partially characterize AGEs used for biochemical studies