Old Remedies in the Biotechnology Age: Moore v. Regents

The California Supreme Court, by excluding a potential remedy, has eliminated many concerns generated by a lower court decision resolving rights to the cell line derived from John Moore\u27s spleen. Yet, the Supreme Court gave little attention to remedies that remain. The authors argue that a constructive trust, a remedy of considerable vintage and great flexibility, could be applied to achieve a just result in such circumstances without affecting the biotechnology world at large

Emergency Department Discharge Instructions: Lessons Learned through Developing New Patient Education Materials

Our multidisciplinary team developed a new set of discharge instructions for five common emergency department diagnoses using recommended tools for creating literacy-appropriate and patient-centered education materials. We found that the recommended tools for document creation were essential in constructing the new instructions. However, while the tools were necessary, they were not sufficient. This paper describes the insights gained and lessons learned in this document creation process

AGN candidates in the VVV near-IR galaxy catalogue

© 2023 The Author(s). Published by Oxford University Press on behalf of Royal Astronomical Society. This is the accepted manuscript version of an article which has been published in final form at https://doi.org/10.1093/mnras/stad490The goal of this work is to search for Active Galactic Nuclei (AGN) in the Galactic disc at very low latitudes with |b|< 2°. For this, we studied the five sources from the VVV near-infrared (IR) galaxy catalogue that have also WISE counterparts and present variability in the VIrac VAriable Classification Ensemble (VIVACE) catalogue. In the near-IR colour-colour diagrams, these objects have in general redder colours compared to the rest of the sources in the field. In the mid-IR ones, they are located in the AGN region, however, there is a source that presents the highest interstellar extinction and different mid-IR colours to be a young stellar object (YSO). We also studied the source variability using two different statistical methods. The fractional variability amplitude σrms ranges from 12.6 to 33.8, being in concordance with previous results found for type-1 AGNs. The slopes of the light curves are in the range (2.6 - 4.7) × 10-4 mag d-1, also in agreement with results reported on quasars variability. The combination of all these results suggest that four galaxies are type-1 AGN candidates, whereas the fifth source likely a YSO candidate.Peer reviewe

High energy gamma-ray sources in the VVV survey - II. The AGN counterparts

© 2024 The Author(s). Published by Oxford University Press on behalf of Royal Astronomical Society. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/We identified Active Galactic Nuclei (AGN) candidates as counterparts to unidentified gamma-ray sources (UGS) from the Fermi-LAT Fourth Source Catalogue at lower Galactic latitudes. Our methodology is based on the use of near- and mid-infrared photometric data from the VISTA Variables in the Vía Láctea (VVV) and Wide-field Infrared Survey Explorer (WISE) surveys. The AGN candidates associated with the UGS occupy very different regions from the stars and extragalactic sources in the colour space defined by the VVV and WISE infrared colours. We found 27 near-infrared AGN candidates possibly associated with 14 Fermi-LAT sources using the VVV survey. We also found 2 blazar candidates in the regions of 2 Fermi-LAT sources using WISE data. There is no match between VVV and WISE candidates. We have also examined the Ks light curves of the VVV candidates and applied the fractional variability amplitude (σrms) and the slope of variation in the Ks passband to characterise the near-infrared variability. This analysis shows that more than 85 per cent of the candidates have slopes in the Ks passband \gt;10−4 mag/day and present σrms values consistent with a moderate variability. This is in good agreement with typical results seen from type-1 AGN. The combination of YJHKs colours and Ks variability criteria was useful for AGN selection, including its use in identifying counterparts to Fermi γ-ray sources.Peer reviewe

High energy gamma-ray sources in the VVV survey -- II. The AGN counterparts

We identified Active Galactic Nuclei (AGN) candidates as counterparts to unidentified gamma-ray sources (UGS) from the Fermi-LAT Fourth Source Catalogue at lower Galactic latitudes. Our methodology is based on the use of near- and mid-infrared photometric data from the VISTA Variables in the V\'ia L\'actea (VVV) and Wide-field Infrared Survey Explorer (WISE) surveys. The AGN candidates associated with the UGS occupy very different regions from the stars and extragalactic sources in the colour space defined by the VVV and WISE infrared colours. We found 27 near-infrared AGN candidates possibly associated with 14 Fermi-LAT sources using the VVV survey. We also found 2 blazar candidates in the regions of 2 Fermi-LAT sources using WISE data. There is no match between VVV and WISE candidates. We have also examined the K$_\mathrm{s}$ light curves of the VVV candidates and applied the fractional variability amplitude ($\mathrm{\sigma_{rms}}$) and the slope of variation in the K$_\mathrm{s}$ passband to characterise the near-infrared variability. This analysis shows that more than 85% of the candidates have slopes in the K$_\mathrm{s}$ passband $> 10^{-4}$ mag/day and present $\mathrm{\sigma_{rms}}$ values consistent with a moderate variability. This is in good agreement with typical results seen from type-1 AGN. The combination of YJHK$_\mathrm{s}$ colours and K$_\mathrm{s}$ variability criteria was useful for AGN selection, including its use in identifying counterparts to Fermi $\gamma$-ray sources.Comment: 16 pages, 19 figures, 4 tables. Accepted for publication in Monthly Notices of the Royal Astronomical Society (MNRAS

The key action of estradiol and progesterone enables GnRH delivery during gestation in the South American plains vizcacha, Lagostomus maximus

The South American plains vizcacha, Lagostomus maximus, is the only mammal described so far that shows expression of estrogen receptors (ERs) and progesterone receptors (PRs) in gonadotropin-releasing hormone (GnRH) neurons. This animal therefore constitutes an exceptional model for the study of the effect of steroid hormones on the modulation of the hypothalamic-pituitary-ovarian (HPO) axis. By using both in vivo and ex vivo approaches, we have found that pharmacological doses of progesterone (P4) and estradiol (E2) produced an inhibition in the expression of hypothalamic GnRH, while physiological doses produced a differential effect on the pulsatile release frequency or genomic expression of GnRH. Our ex vivo experiment indicates that a short-term effect of E2 modulates the frequency of GnRH release pattern that would be associated with membrane ERs. On the other hand, our in vivo approach suggests that a long-term effect of E2, acting through the classical nuclear ERs-PRs pathway, would produce the modification of GnRH mRNA expression during the GnRH pre-ovulatory surge. Particularly, P4 induced a rise in GnRH mRNA expression and protein release with a decrease in its release frequency. These results suggest different levels of action of steroid hormones on GnRH modulation. We conclude that the fine action of E2 and P4 constitute the key factor to enable the hypothalamic activity during the pregnancy of this mammal.Fil: Inserra, Pablo Ignacio Felipe. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Charif, Santiago Elías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Fidel, Victoria. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Giacchino, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Schmidt, Alejandro Raúl. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Villarreal, Federico M.. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Proietto, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Cortasa, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Corso, María Clara. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gariboldi, María Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Leopardo, Noelia Paola. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fraunhoffer, Nicolás A.. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Di Giorgio, Noelia Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Lux Lantos, Victoria A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Halperin, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Vitullo, Alfredo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Dorfman, Verónica Berta. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Hubble Frontier Field Photometric Catalogues of Abell 370 and RXC J2248.7-4431: Multiwavelength photometry, photometric redshifts, and stellar properties

This paper presents multiwavelength photometric catalogues of the last two Hubble Frontier Fields (HFF), the massive galaxy clusters Abell 370 and RXC J2248.7-4431. The photometry ranges from imaging performed on the Hubble Space Telescope (HST) to ground based Very Large Telescope (VLT) and Spitzer/IRAC, in collaboration with the ASTRODEEP team, and using the ASTRODEEP pipeline. While the main purpose of this paper is to release the catalogues, we also perform, as a proof of concept, a brief analysis of z > 6 objects selected using drop-out method, as well as spectroscopically confirmed sources and multiple images in both clusters. While dropout methods yield a sample of high-z galaxies, the addition of longer wavelength data reveals that as expected the samples have substantial contamination at the ~30-45% level by dusty galaxies at lower redshifts. Furthermore, we show that spectroscopic redshifts are still required to unambiguously determine redshifts of multiply imaged systems. Finally, the now publicly available ASTRODEEP catalogues were combined for all HFFs and used to explore stellar properties of a large sample of 20,000 galaxies across a large photometric redshift range. The powerful magnification provided by the HFF clusters allows us an exploration of the properties of galaxies with intrinsic stellar masses as low as $M_* \gtrsim 10^7M_{\odot}$ and intrinsic star formation rates \mbox{SFRs}\sim 0.1\mbox{-}1M_\odot/\mbox yr at z > 6.Comment: Replaced to match the accepted versio

Cellular HIV-1 DNA Levels in Drug Sensitive Strains Are Equivalent to Those in Drug Resistant Strains in Newly-Diagnosed Patients in Europe

Background HIV-1 genotypic drug resistance is an important threat to the success of antiretroviral therapy and transmitted resistance has reached 9% prevalence in Europe. Studies have demonstrated that HIV-1 DNA load in peripheral blood mononuclear cells (PBMC) have a predictive value for disease progression, independently of CD4 counts and plasma viral load. Methodology/Principal Findings Molecular-beacon-based real-time PCR was used to measure HIV-1 second template switch (STS) DNA in PBMC in newly-diagnosed HIV-1 patients across Europe. These patients were representative for the HIV-1 epidemic in the participating countries and were carrying either drug-resistant or sensitive viral strains. The assay design was improved from a previous version to specifically detect M-group HIV-1 and human CCR5 alleles. The findings resulted in a median of 3.32 log10HIV-1copies/106PBMC and demonstrated for the first time no correlation between cellular HIV-1 DNA load and transmitted drug-resistance. A weak association between cellular HIV-1 DNA levels with plasma viral RNA load and CD4+T-cell counts was also reconfirmed. Co-receptor tropism for 91% of samples, whether or not they conferred resistance, was CCR5. A comparison of pol sequences derived from RNA and DNA, resulted in a high similarity between the two. Conclusions/Significance An improved molecular-beacon-based real-time PCR assay is reported for the measurement of HIV-1 DNA in PBMC and has investigated the association between cellular HIV-1 DNA levels and transmitted resistance to antiretroviral therapy in newly-diagnosed patients from across Europe. The findings show no correlation between these two parameters, suggesting that transmitted resistance does not impact disease progression in HIV-1 infected individuals. The CCR5 co-receptor tropism predominance implies that both resistant and non-resistant strains behave similarly in early infection. Furth

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space. While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes, vast areas of the tropics remain understudied. In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity, but it remains among the least known forests in America and is often underrepresented in biodiversity databases. To worsen this situation, human-induced modifications may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge, it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Temporal variability in foraminiferal morphology and geochemistry at the West Antarctic Peninsula: a sediment trap study

The West Antarctic Peninsula (WAP) exhibits strong spatial and temporal oceanographic variability, resulting in highly heterogeneous biological productivity. Calcifying organisms that live in the waters off the WAP respond to temporal and spatial variations in ocean temperature and chemistry. These marine calcifiers are potentially threatened by regional climate change with waters already naturally close to carbonate undersaturation. Future projections of carbonate production in the Southern Ocean are challenging due to the lack of historical data collection and complex, decadal climate variability. Here we present a 6-year-long record of the shell fluxes, morphology and stable isotope variability of the polar planktic foraminifera Neogloboquadrina pachyderma (sensu stricto) from near Palmer Station, Antarctica. This species is fundamental to Southern Ocean planktic carbonate production as it is one of the very few planktic foraminifer species adapted to the marine polar environments. We use these new data to obtain insights into its ecology and to derive a robust assessment of the response of this polar species to environmental change. Morphology and stable isotope composition reveal the presence of different growth stages within this tightly defined species. Inter- and intra-annual variability of foraminiferal flux and size is evident and driven by a combination of environmental forcing parameters, most importantly food availability, temperature and sea ice duration and extent. Foraminiferal growth occurs throughout the austral year and is influenced by environmental change, a large portion of which is driven by the Southern Annular Mode and El Niño–Southern Oscillation. A distinct seasonal production is observed, with the highest shell fluxes during the warmest and most productive months of the year. The sensitivity of calcifying foraminifera to environmental variability in this region, from weeks to decades, has implications both for their response to future climatic change and for their use as palaeoclimate indicators. A longer ice-free season could increase carbonate production in this region at least while carbonate saturation is still high enough to allow for thick tests to grow