4,049 research outputs found
Glutathione treatment protects the rat liver against injury after warm ischemia and Kupffer cell activation
Background/Aim: The generation of reactive oxygen species by activated Kupffer cells (KC) may contribute to reperfusion injury of the liver during liver transplantation or resection. The aim of our present studies was to investigate (1) prevention of hepatic reperfusion injury after warm ischemia by administration of the antioxidant glutathione (GSH) and (2) whether GSH confers protection through influences on KC toxicity. Methods: Isolated perfused rat livers were subjected to 1 h of warm ischemia followed by 90 min of reperfusion without (n = 5) or with GSH or catalase (n = 4-5 each). Selective KC activation by zymosan (150 mug/ml) in continuously perfused rat livers was used to investigate KC-related liver injury. Results: Postischemic infusion of 0.1, 0.5, 1.0 and 2.0 mM GSH, but not 0.05 mM GSH prevented reperfusion injury after warm ischemia as indicated by a marked reduction of sinusoidal LDH efflux by up to 83 +/- 13% (mean +/- SD; p < 0.05) and a concomitant significant improvement of postischemic bile flow by 58 +/- 27% (p < 0.05). A similar protection was conveyed by KC blockade with gadolinium chloride indicating prevention of KC-related reperfusion injury by postischemic GSH treatment. Postischemic treatment with catalase (150 U/ml) resulted in a reduction of LDH efflux by 40 +/- 9% (p < 0.05). Accordingly, catalase as well as GSH (0.1-2.0 mM) nearly completely prevented the increase in LDH efflux following selective :KC activation by zymosan in continously perfused rat livers. Conclusion: Postischemic administration of GSH protects the liver against reperfusion injury after warm ischemia. Detoxification of KC-derived hydrogen peroxide seem to be an important feature of the protective mechanisms. Copyright (C) 2002 S. Karger AG, Basel
Control strategies for integration of electric motor assist and functional electrical stimulation in paraplegic cycling: Utility for exercise testing and mobile cycling
AIM: The aim of this study was to investigate feedback
control strategies for integration of electric motor assist and functional electrical stimulation (FES) for paraplegic cycling, with particular focus on development of a testbed for exercise testing in FES cycling, in which both cycling cadence and workrate are simultaneously well controlled and contemporary physiological measures of exercise performance derived. A second aim was
to investigate the possible benefits of the approach for mobile, recreational cycling.
METHODS: A recumbent tricycle with an auxiliary electric motor is used, which is adapted for paraplegic users, and instrumented for stimulation control. We propose a novel integrated control strategy which simultaneously provides feedback control of leg power output (via automatic adjustment of stimulation intensity) and cycling cadence (via electric motor control). Both loops are
designed using system identification and analytical (model-based) feedback design methods. Ventilatory and pulmonary gas exchange response profiles are derived using a portable system for real-time breath-by-breath acquisition.
RESULTS:We provide indicative results from one paraplegic subject in which a series of feedback-control tests illustrate accurate control of cycling cadence, leg power control, and external disturbance rejection. We also provide physiological response profiles from a submaximal exercise step test and a maximal incremental exercise test, as facilitated by the control strategy.
CONCLUSION: The integrated control strategy is effective in facilitating
exercise testing under conditions of well-controlled cadence
and power output. Our control approach significantly extends the
achievable workrate range and enhances exercise-test sensitivity
for FES cycling, thus allowing a more stringent characterization
of physiological response profiles and estimation of key parameters
of aerobic function.We further conclude that the control approach
can significantly improve the overall performance of mobile recreational
cycling
GSH Attenuates Organ Injury and Improves Function after Transplantation of Fatty Livers
Ischemia-reperfusion injury (IRI) is increased after transplantation of steatotic livers. Since those livers are increasingly used for transplantation, protective strategies must be developed. Reactive oxygen species (ROS) play a key role in hepatic IRI. In lean organs, glutathione (GSH) is an efficient scavenger of ROS, diminishing IRI. The aim of this study was to evaluate whether GSH also protects steatotic allografts from IRI following transplantation. Fatty or lean livers were explanted from 10-week-old obese or lean Zucker rats and preserved (obese 4 h, lean 24 h) in hypothermic University of Wisconsin solution. Arterialized liver transplantation was then performed in lean syngeneic Zucker rats. Recipients of fatty livers were treated with GSH (200 mu mol/h/kg) or saline during reperfusion (2 h, n = 5). Parameters of hepatocellular damage and bile flow were measured. Transplantation of steatotic livers enhanced early reperfusion injury compared to lean organs as measured by increased aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase plasma levels. Bile flow was also reduced in steatotic grafts. Intravenous administration of GSH effectively decreased liver damage in fatty allografts and resulted in improved bile flow. Intravenous application of GSH effectively reduces early IRI in steatotic allografts and improves recovery of these marginal donor organs following transplantation. Copyright (C) 2010 S. Karger AG, Base
Edaq530: a transparent, open-end and open-source measurement solution in natural science education
We present Edaq530, a low-cost, compact and easy-to-use digital measurement
solution consisting of a thumb-sized USB-to-sensor interface and a measurement
software. The solution is fully open-source, our aim being to provide a viable
alternative to professional solutions. Our main focus in designing Edaq530 has
been versatility and transparency. In this paper, we shall introduce the
capabilities of Edaq530, complement it by showing a few sample experiments, and
discuss the feedback we have received in the course of a teacher training
workshop in which the participants received personal copies of Edaq530 and
later made reports on how they could utilise Edaq530 in their teaching
RV POSEIDON Cruise Report POS420 COWACSS Biological observation and sampling of cold-water corals to investigate impacts on climate change
Trondheim – (Kristiansund) – Kiel
08. – (25.) – 30.09.201
Prospects for measuring the 229Th isomer energy using a metallic magnetic microcalorimeter
The Thorium-229 isotope features a nuclear isomer state with an extremely low
energy. The currently most accepted energy value, 7.8 +- 0.5 eV, was obtained
from an indirect measurement using a NASA x-ray microcalorimeter with an
instrumental resolution 26 eV. We study, how state-of-the-art magnetic metallic
microcalorimeters with an energy resolution down to a few eV can be used to
measure the isomer energy. In particular, resolving the 29.18 keV doublet in
the \gamma-spectrum following the \alpha-decay of Uranium-233, corresponding to
the decay into the ground and isomer state, allows to measure the isomer
transition energy without additional theoretical input parameters, and increase
the energy accuracy. We study the possibility of resolving the 29.18 keV line
as a doublet and the dependence of the attainable precision of the energy
measurement on the signal and background count rates and the instrumental
resolution.Comment: 32 pages, 8 figures, eq. (3) correcte
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