Implementación de talleres extracurriculares para favorecer el proceso de aprendizaje en el área matemática de la FCE de la UNRC

Este trabajo presenta y analiza la implementación de una estrategia institucional en las asignaturas Análisis Matemático I y Análisis Matemático II de las carreras que se dictan en la Facultad de Ciencias Económicas de la Universidad Nacional de Río Cuarto. La misma está basada en una metodología de Taller y en el uso de TIC ( tecnologías de la información y de la comunicación). Esta propuesta, destinada a estudiantes que habían cursado las asignaturas y quedaron libres por parcial, tuvo como objetivo desarrollar estrategias cognitivas y al mismo tiempo favorecer la permanencia de los estudiantes en las mencionadas asignaturas. Consistieron en interpretar, debatir y responder grupal y colaborativamente, consignas escritas, extraídas de exámenes y actividades, culminando con la puesta en común de los grupos participantes. La valoración global de la experiencia es muy positiva, no obstante, se señala la dificultad de integrar herramientas TIC en el proceso de enseñanza y aprendizaje.Fil: Scattolini, Nancy.Fil: Cabrera, Silvia.Fil: Panella, Susana.Fil: Clerici, Jimena

Implementación de talleres extracurriculares para favorecer el proceso de aprendizaje en el área matemática de la FCE de la UNRC

Este trabajo presenta y analiza la implementación de una estrategia institucional en las asignaturas Análisis Matemático I y Análisis Matemático II de las carreras que se dictan en la Facultad de Ciencias Económicas de la Universidad Nacional de Río Cuarto. La misma está basada en una metodología de Taller y en el uso de TIC ( tecnologías de la información y de la comunicación). Esta propuesta, destinada a estudiantes que habían cursado las asignaturas y quedaron libres por parcial, tuvo como objetivo desarrollar estrategias cognitivas y al mismo tiempo favorecer la permanencia de los estudiantes en las mencionadas asignaturas. Consistieron en interpretar, debatir y responder grupal y colaborativamente, consignas escritas, extraídas de exámenes y actividades, culminando con la puesta en común de los grupos participantes. La valoración global de la experiencia es muy positiva, no obstante, se señala la dificultad de integrar herramientas TIC en el proceso de enseñanza y aprendizaje.Fil: Scattolini, Nancy.Fil: Cabrera, Silvia.Fil: Panella, Susana.Fil: Clerici, Jimena

Simvastatin Rapidly and Reversibly Inhibits Insulin Secretion in Intact Single-Islet Cultures

open10Epidemiological studies suggest that statins may promote the development or exacerbation of diabetes, but whether this occurs through inhibition of insulin secretion is unclear. This lack of understanding is partly due to the cellular models used to explore this phenomenon (cell lines or pooled islets), which are non-physiologic and have limited clinical transferability.openScattolini, Valentina; Luni, Camilla; Zambon, Alessandro; Galvanin, Silvia; Gagliano, Onelia; Ciubotaru, Catalin Dacian; Avogaro, Angelo; Mammano, Fabio; Elvassore, Nicola; Fadini, Gian PaoloScattolini, Valentina; Luni, Camilla; Zambon, Alessandro; Galvanin, Silvia; Gagliano, Onelia; Ciubotaru, CATALIN DACIAN; Avogaro, Angelo; Mammano, Fabio; Elvassore, Nicola; Fadini, GIAN PAOL

Uso de simuladores de negocios como práctica interdisciplinaria en la asignatura Tecnología de la Información de la FCE-UNRC

El presente trabajo trata sobre los simuladores de negocios utilizados en la Asignatura Tecnología de la Información que imparte la Facultad de Ciencias Económicas (FCE) de la Universidad Nacional de Río Cuarto (UNRC). Se pretende describirlos como herramienta tecnológica digital, comunicacional y pedagógica en la aplicación de conceptos de gestión administrativa e información contable, también valorarlos como mediadores en los procesos educativos. El estudio se sustenta en la investigación cualitativa, implicando un proceso inductivo de conocimiento. Como técnicas de recolección de datos se utilizó la observación participante y la entrevista en profundidad individual y grupal, siendo la unidad de análisis un grupo formado por el Docente Responsable de dicha Asignatura junto a treinta alumnos pertenecientes a las carreras Contador Público y Licenciatura en Administración de la FCE. Los resultados obtenidos dan cuenta del entusiasmo de los estudiantes ante la posibilidad de poner en práctica sus conocimientos teóricos aprendidos a lo largo de la carrera y coincidieron, tanto estudiantes como docente, que el simulador permitió integrar interdisciplinariamente los contenidos de las diferentes vertientes de formación técnica del graduado, con práctica informatizada de problemas propios de la realidad profesional y empresarial, lo que constituyó un ejemplo positivo para replicar la experiencia.Dirección de Educación a Distancia, Innovación en el aula y TIC (EAD

Fungos associados a Platanus x acerifolia no Uruguai e indicadores de probabilidade de falha

In the last few years, the interest in keeping the city trees healthy has increased in order to improve their survival and minimize claims due to potential accidents. The pest and diseases, the pollution, and the climate change together with the little genetic diversity of trees in urban areas are some of the factors that contribute to increase the likelihood of death and/or failure of trees in the cities. This work is part of a sanitary and risk of failure assessment of plane street trees (Platanus x acerifolia) carried out between 2019 and 2020. A random sample of 10 city blocks and their 193 plane trees was selected. In these, the presence of cankers, abnormal bark colorations, deformations, and a series of structural attributes that determine likelihood of failure variables were registered. The proportion of individuals with each symptom and the severity main index (SMI) were calculated as a weighted average of the different severity (SEV) levels in the total of evaluated plants. The severity indices were determined according to trunk or branches circumference and the portion of the tree affected (1st, 2nd or 3rd portion from the base). Deformations presented the main incidence (0.6), SMI (1.68) and a correlation with the presence of damages and human injuries. The presence of cankers and reddish bark were the symptoms that most affected the density of the crowns.En los últimos años el interés por el mantenimiento de la salud de los árboles de la ciudad ha ido incrementando para mejorar su sobrevivencia, así como para minimizar reclamaciones por posibles accidentes. Las enfermedades y las plagas, la polución y la aceleración del cambio climático junto con la poca diversidad genética de los árboles en zonas urbanas son algunos de los factores que contribuyen a aumentar la probabilidad de muerte y/o falla de árboles en las ciudades. Este trabajo es parte de una evaluación sanitaria y de riesgo de plátanos (Platanus x acerifolia) ubicados en veredas, realizada entre 2019 y 2020. Se seleccionó una muestra al azar de 10 manzanas con 193 plátanos. En estos, se registró la presencia de cancros, coloraciones anormales de corteza, deformaciones y una serie de variables estructurales indicadoras de probabilidad de falla. Se calculó la proporción de individuos con cada síntoma y el índice medio de severidad (IMS) como un promedio ponderado de los diferentes niveles de severidad (SEV) en el total de las plantas evaluadas. Los índices de severidad se determinaron en función de la circunferencia del tronco o las ramas y de los tercios afectados del árbol (1.er, 2.do y 3.er tercio desde la base). Las deformaciones presentaron los valores máximos de incidencia (0,6), de IMS (1,68) y correlación con la presencia de heridas y daños antrópicos. La presencia de cancros y corteza rojiza fueron los síntomas que más afectaron la densidad de las copas.Nos últimos anos tem aumentado o interesse na manutenção da arborização urbana a fim de melhorar sua sobrevivência e minimizar reclamações devido a potenciais acidentes. Pragas e doenças, poluição, y mudanças climáticas junto com a pouca diversidade genética das árvores em áreas urbanas são alguns dos fatores que contribuem para aumentar a probabilidade de morte e ou falha de árvores nas cidades. Este trabalho faz parte de uma avaliação sanitária e de risco de falha de plátanos localizados em calçadas (Platanus x acerifolia), realizada entre 2019 e 2020. Foi selecionada uma amostra aleatória de 10 quarteirões e seus 193 plátanos. Para cada indivíduo, foi registrada a presença de cancros, colorações anormais da casca, deformações e também uma série de atributos estruturais indicadores ​​de probabilidade de falha. Calculou-se a proporção de indivíduos com cada sintoma e o índice principal de severidade (SMI) como média ponderada dos diferentes níveis de severidade (SEV), no total de plantas avaliadas. Os índices de severidade foram determinados de acordo com a circunferência do tronco ou galhos e a porção da árvore afetada (1ª, 2ª ou 3ª porção da base). As deformações apresentaram os valores máximos de incidência (0,6), SMI (1,68) e correlação com presença de danos e lesões humanas. A presença de cancro e casca avermelhada foram os sintomas que mais afetaram a densidade das copas

Cross-sectional study of hepatitis E virus (HEV) circulation in Italian pig farms

OHEJP Project: BIOPIGEE Foodborne transmission is considered the main way of spreading zoonotic hepatitis E virus (HEV) infection in Europe. In recent years, the human cases of hepatitis E in subjects without history of travel in endemic areas have raised, suggesting that domestic HEV transmission is increasing. Pork products with or without liver, are often indicated as the source of many human foodborne HEV cases as well as small outbreaks. Pigs are recognized as the main reservoir of the zoonotic HEV-3 genotype, the most frequently detected in human cases in the EU. In the absence of a harmonized surveillance of HEV circulation, data on prevalence are heterogeneous but confirm a widespread circulation of HEV-3 in pig herds across EU. HEV-3 can pass through the food chain from farm to fork when infected animals are slaughtered. In Italy, several studies reported the circulation of HEV-3 in pig farms, but results are heterogeneous due to dierent methodologies applied. In the present study, we performed a survey over 51 pig herds belonging to three main types of farms: breeding, fattening and farrow-to- finish. HEV-RNA was analyzed by broad range Real-time RT-PCR on 20 samples for each farm, obtained by pooling together feces from 10 individuals. Overall, HEV RNA was confirmed on 150 fecal pooled samples out of 1,032 (14.5%). At least one positive pooled sample was detected from 18 farms out of 51 tested (35.3%). By lowering the number of infected pigs at primary production, the risk of HEV-3 entering into the food chain can be reduced. Hence, information on HEV circulation in herds is highly relevant for choosing preventive measures and deserves development of a monitoring program and further investigations

Bacteriophage therapy to reduce colonization of campylobacter jejuni in broiler chickens before slaughter

Campylobacteriosis is the most commonly reported gastrointestinal disease in humans. Campybacter jejuni is the main cause of the infection, and bacterial colonization in broiler chickens is widespread and difficult to prevent, leading to high risk of occurrence in broiler meat. Phage therapy represents an alternative strategy to control Campylobacter in poultry. The aim of this work was to assess the efficacy of two field-isolated bacteriophages against experimental infections with an anti-microbial resistant (AMR) Campylobacter jejuni strain. A two-step phage application was tested according to a specific combination between chickens’ rearing time and specific multiplicities of infections (MOIs), in order to reduce the Campylobacter load in the animals at slaughtering and to limit the development of phage-resistant mutants. In particular, 75 broilers were divided into three groups (A, B and C), and phages were administered to animals of groups B and C at day 38 (Φ 16-izsam) and 39 (Φ 7-izsam) at MOI 0.1 (group B) and 1 (group C). All broilers were euthanized at day 40, and Campylobacter jejuni was enumerated in cecal contents. Reductions in Campylobacter counts were statistically significant in both group B (1 log10 colony forming units (cfu)/gram (gr)) and group C (2 log10 cfu/gr), compared to the control group. Our findings provide evidence about the ability of phage therapy to reduce the Campylobacter load in poultry before slaughtering, also associated with anti-microbial resistance pattern

Biosecurity measures to control hepatitis E virus on European pig farms

Hepatitis E virus (HEV) genotype 3 is a prevalent zoonotic pathogen in European pig farms, posing a significant public health risk primarily through the foodborne route. The study aimed to identify effective biosecurity measures for controlling HEV transmission on pig farms, addressing a critical gap in current knowledge. Utilizing a cross-sectional design, fecal samples from gilts, dry sows, and fatteners were collected on 231 pig farms of all farm types across nine European countries. Real-time RT-PCR was employed to test these samples for HEV. Simultaneously, a comprehensive biosecurity questionnaire captured data on various potential measures to control HEV. The dependent variable was HEV risk, categorized as lower or higher based on the percentage of positive pooled fecal samples on each farm (25% cut-off). The data were analyzed using generalized linear models (one for finisher samples and one for all samples) with a logit link function with country and farm type as a priori fixed factors. The results of the final multivariable models identified key biosecurity measures associated with lower HEV risk, which were the use of a hygienogram in the breeding (OR: 0.06, p = 0.001) and/or fattening area after cleaning (OR: 0.21, p = 0.019), the presence of a quarantine area (OR: 0.29, p = 0.025), testing and/or treating purchased feed against Salmonella (OR: 0.35, p = 0.021), the presence of other livestock species on the farm, and having five or fewer persons in charge of the pigs. Contrary to expectations, some biosecurity measures were associated with higher HEV risk, e.g., downtime of 3 days or longer after cleaning in the fattening area (OR: 3.49, p = 0.005) or mandatory handwashing for farm personnel when changing barn sections (OR: 3.4, p = 0.026). This novel study unveils critical insights into biosecurity measures effective in controlling HEV on European pig farms. The identification of both protective and risk-associated measures contributes to improving strategies for managing HEV and underscores the complexity of biosecurity in pig farming

Toxin genotyping of Clostridium perfringens strains using a polymerase chain reaction protocol

A polymerase chain reaction protocol consisting of a multiplex to identify the cpa, cpb1, cpetx, cpi genes and a duplex to identify the cpe and cpb2 genes encoding for a, b1, e, i, enterotoxin and b2 toxins, respectively, was applied to DNA extracted from two collections of Clostridium perfringens strains. The first collection involved 19 isolates from rabbits. The second collection of 41 isolates came from routine necropsies. The cpa gene alone, or in association with the cpb2 gene, was detected in all DNA samples examined. The cpa gene, together with cpb2 gene, were detected in seven of the rabbit C. perfringens strains (36.8%) and in nine isolates from necropsies (21.9%). The cpa gene was found in 63.2% of rabbit strains and 76.9% of strains from other animal species. In rabbits, the pathological lesions associated with C. perfringens detection were predominantly forms of non-inflammatory enteropathies. In other species, C. perfringens was mainly associated with congestive-haemorrhagic enteropathy, but also with fatal traumatic lesions, degenerative diseases and organs with post-mortem autolysis. No clear correlation was observed between detection of b2 toxin gene and species-specific pathological features

Tossino-genotipizzazione di Clostridium perfringens mediante protocollo di reazione a catena della polimerasi

Il DNA estratto da due raccolte di ceppi di Clostridium perfringens è stato sottoposto a un protocollo di reazione a catena della polimerasi (PCR) mediante PCR multiplex per evidenziare la presenza di geni cpa, cpb1, cpetx, cpi e PCR duplex per i geni cpe e cpb2 che codificano, rispettivamente, le tossine α, β1, ε, ι, enterotossina e β2. Le due raccolte hanno riguardato ceppi di C. perfringens, 19 di origine cunicola e 41 provenienti da specie animali differenti. Tutti i campioni di DNA sono risultati positivi per il gene cpa, rilevato singolarmente o in combinazione con il gene cpb2. L’evidenziazione del gene cpa ha caratterizzato il 63,2% dei ceppi di origine cunicola e il 76,9% dei ceppi isolati nelle altre specie animali. Il genotipo cpa+cpb2 è risultato presente in 7 ceppi (36,8%) di origine cunicola e in 9 ceppi (21,9%) provenienti da specie animali differenti. Nel coniglio, le lesioni patologiche associate all’isolamento di C. perfringens sono risultate, principalmente, forme di enteropatie non infiammatorie. Nelle specie diverse dal coniglio, C. perfringens è stato isolato prevalentemente in animali con enteropatie a carattere congestizio-emorragico ma anche con lesioni traumatiche fatali, malattie degenerative e organi in autolisi post-mortem. Nessuna chiara correlazione è stata osservata tra la positività per il gene della tossina β2 e quadri patologici specie-specifici