Degradation of a cAMP-binding protein of Dictyostelium discoideum by an endogenous protease

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/25251/1/0000694.pd

Area-level incarceration and STI risk among a cohort of justice-involved adolescents and adults

Background: Living in areas of high incarceration is associated with increased risk of STI; however, STI risk with respect to both this area-level exposure and an individual’s involvement with the justice system is not known. Objective: Among individuals before and after arrest or incarceration, assess the association between area-level incarceration rates and risk of chlamydia, gonorrhea, or syphilis. Methods: Retrospective cohort study of individuals living in Marion County (Indianapolis), Indiana who were arrested or in jail, prison, juvenile detention, or juvenile prison between 2005-2008 (N=97,765). Area-level incarceration exposure was defined by the proportion of person-days incarcerated among the total population*365 within a census block group. A 1-year period was assessed before and after a randomly-selected arrest/incarceration per person. Multivariable logistic regression, controlling for age, race, STI history, and year, was performed to assess chlamydia, gonorrhea, or syphilis risk by quartile area-level incarceration exposure, adjusting for individual clustering and stratifying by gender. Results: Area-level incarceration was associated with increased odds of each STI, with a dose response relationship particularly among those with an arrest or jail stay. Women with a history of arrest or jail/prison stay and living in high incarceration areas had higher odds of STI, compared to men with comparable incarceration history and living in similar areas

Identification and characterization of the adenosine 3',5'-cyclic monophosphate binding proteins appearing during the development of Dictyostelium discoideum

A photosensitive, radioactive analogue of cyclic adenosine monophosphate, 8-azido-adenosine 3',5'-[32P]monophosphate (8-N3-cyclic AMP), was used to label the cyclic AMP binding proteins of Dictyostelium discoideum. During development cytosolic proteins appear which are specifically labeled by the photoaffinity agent. The proteins are developmentally regulated since they are only found in starved, developing cells. Unlabeled cyclic AMP competes specifically with the labeled analogue for protein binding sites in contrast to unlabeled 5'-AMP which does not compete. A mutant which develops spores but is deficient in stalk cell production produces a different set of cyclic AMP binding proteins from the parent strain.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/23243/1/0000176.pd

Test positivity for chlamydia, gonorrhea, and syphilis infection among a cohort of individuals released from jail in Marion County, Indiana

BACKGROUND: Individuals entering jails have high rates of sexually transmitted infections (STI), but there are few data on STI in the postincarceration period. This study aimed to describe rates of chlamydia, gonorrhea, and syphilis infection among individuals released from Marion County (Indianapolis), Indiana jails. METHODS: We conducted a retrospective cohort study of individuals incarcerated in Marion County, Indiana jails from 2003 to 2008 (n = 118,670). We linked county jail and public health data to identify individuals with positive STI test results in the 1 year after release from jail. Rates per 100,000 individuals and Cox proportional hazard analyses were performed for each STI, stratified by demographic, STI, and jail characteristics. RESULTS: We found significantly higher rates of STI in this cohort than in the general population, with rates in the 1 year after release being 2 to 7 times higher for chlamydia, 5 to 24 times higher for gonorrhea, and 19 to 32 times higher for syphilis compared with rates in the general population. Characteristics most associated with increased risk of a positive STI test result among this cohort were younger age for chlamydia and gonorrhea, older age for syphilis, black race for men, being jailed for prostitution for women, history of STI, and history of prior incarceration. CONCLUSIONS: This study found high rates of STIs among a cohort of individuals recently released from jail and identified a number of risk factors. Further study is needed to improve targeted STI testing and treatment among this high-risk population

Cost-benefit analysis of extending support to domestic abuse victims with NRPF: a technical report for the Domestic Abuse Commissioner

This report provides the technical underpinning to the report by the Domestic Abuse Commissioner’s Report1 laid before Parliament in December 2022. That report was in response to the Home Office request that she set out ‘the gaps in evidence available to the Home Office on support for Migrant Victims of Domestic Abuse, by establishing the number of victims and survivors of domestic abuse who have no recourse to public funds, the cost of supporting those who need support, and the cost benefit of such interventions.2’ LSE was asked by the Commissioner to provide a detailed technical analysis to underpin her response. This LSE report provides our views on relevant evidence but no policy recommendations. Such recommendations are made in the Commissioner’s report, having considered our evidence alongside the evidence of other stakeholders and people she has consulted. Our analysis required us to make strong assumptions, and there is significant uncertainty around many of them. There is considerable uncertainty in published validated statistics about the numbers of migrants in the UK with each visa status, and even more uncertainty about numbers of undocumented migrants and of ‘visitors’. There is also uncertainty about how many of these migrants currently experience domestic abuse, and an added and independent uncertainty about the proportion of those people who would present at services. We have also had to make various assumptions which affect the costs and benefits. We address and mitigate these and other uncertainties through our sensitivity analysis and additional modelling in this report. In addition, an accompanying Excel workbook sets out in detail our assumptions and how they underpin our conclusions, which allows for further sensitivity analysis and modelling to be done

Distributive Politics and Social Protection in the 21st Century

The University Archives has determined that this item is of continuing value to OSU's history.Dramatic economic and demographic transitions have prompted societies around the world to renegotiate the social bargains underpinning national social protection systems. In the process, social welfare reforms have laid bare deep fault lines of distributive conflict, cleaving societies across generations, income levels, and risk groups. Although considerable scholarly attention has been paid to the distinctive contours of these reforms, much of this inquiry remains bound within regional or national lines. As a step toward bridging these empirical and theoretical gaps, this workshop will bring together scholars engaged in research on social protection and distributive conflict in diverse regions of the world, from Latin America to Europe and Asia. Questions addressed in the workshop will include efforts to understand the longer-term implications of social welfare transformations, while asking what changes in social welfare spending, structure and function will imply for longer-term distributions of political and economic resources, risk and life chances. Lastly, participants in the workshop will ask how the shift of risks from collective social insurance programs to individuals affect social cohesion, and democratic stability.Ohio State University. Mershon Center for International Security StudiesEvent webpage, handou

Microdissection of Shoot Meristem Functional Domains

The shoot apical meristem (SAM) maintains a pool of indeterminate cells within the SAM proper, while lateral organs are initiated from the SAM periphery. Laser microdissection–microarray technology was used to compare transcriptional profiles within these SAM domains to identify novel maize genes that function during leaf development. Nine hundred and sixty-two differentially expressed maize genes were detected; control genes known to be upregulated in the initiating leaf (P0/P1) or in the SAM proper verified the precision of the microdissections. Genes involved in cell division/growth, cell wall biosynthesis, chromatin remodeling, RNA binding, and translation are especially upregulated in initiating leaves, whereas genes functioning during protein fate and DNA repair are more abundant in the SAM proper. In situ hybridization analyses confirmed the expression patterns of six previously uncharacterized maize genes upregulated in the P0/P1. P0/P1-upregulated genes that were also shown to be downregulated in leaf-arrested shoots treated with an auxin transport inhibitor are especially implicated to function during early events in maize leaf initiation. Reverse genetic analyses of asceapen1 (asc1), a maize D4-cyclin gene upregulated in the P0/P1, revealed novel leaf phenotypes, less genetic redundancy, and expanded D4-CYCLIN function during maize shoot development as compared to Arabidopsis. These analyses generated a unique SAM domain-specific database that provides new insight into SAM function and a useful platform for reverse genetic analyses of shoot development in maize

Deconstructing compassionate conservation

Compassionate conservation focuses on 4 tenets: first, do no harm; individuals matter; inclusivity of individual animals; and peaceful coexistence between humans and animals. Recently, compassionate conservation has been promoted as an alternative to conventional conservation philosophy. We believe examples presented by compassionate conservationists are deliberately or arbitrarily chosen to focus on mammals; inherently not compassionate; and offer ineffective conservation solutions. Compassionate conservation arbitrarily focuses on charismatic species, notably large predators and megaherbivores. The philosophy is not compassionate when it leaves invasive predators in the environment to cause harm to vastly more individuals of native species or uses the fear of harm by apex predators to terrorize mesopredators. Hindering the control of exotic species (megafauna, predators) in situ will not improve the conservation condition of the majority of biodiversity. The positions taken by so-called compassionate conservationists on particular species and on conservation actions could be extended to hinder other forms of conservation, including translocations, conservation fencing, and fertility control. Animal welfare is incredibly important to conservation, but ironically compassionate conservation does not offer the best welfare outcomes to animals and is often ineffective in achieving conservation goals. Consequently, compassionate conservation may threaten public and governmental support for conservation because of the limited understanding of conservation problems by the general public