UBVRI photopolarimetry of the long period eclipsing AM Herculis binary V1309

We report simultaneous UBVRI photo-polarimetric observations of the long period (7.98 h) AM Her binary V1309 Ori. The length and shape of the eclipse ingress and egress varies from night to night. We suggest this is due to the variation in the brightness of the accretion stream. By comparing the phases of circular polarization zero-crossovers with previous observations, we confirm that V1309 Ori is well synchronized, and find an upper limit of 0.002 percent for the difference between the spin and orbital periods. We model the polarimetry data using a model consisting of two cyclotron emission regions at almost diametrically opposite locations, and centered at colatitude 35 (deg) and 145 (deg) on the surface of the white dwarf. We also present archive X-ray observations which show that the negatively polarised accretion region is X-ray bright.Comment: 11 pages, 12 figures (2 colour), Fig1 and Fig 4 are in lower resolution than in original paper, accepted for publication in Monthly Notices of the Royal Astronomical Societ

Extreme Kuiper Belt Object 2001 QG298 and the Fraction of Contact Binaries

Extensive time-resolved observations of Kuiper Belt object 2001 QG298 show a lightcurve with a peak-to-peak variation of 1.14 +-0.04 magnitudes and single-peaked period of 6.8872 +- 0.0002 hr. The mean absolute magnitude is 6.85 magnitudes which corresponds to a mean effective radius of 122 (77) km if an albedo of 0.04 (0.10) is assumed. This is the first known Kuiper Belt object and only the third minor planet with a radius > 25 km to display a lightcurve with a range in excess of 1 magnitude. We find the colors to be typical for a Kuiper Belt object (B-V = 1.00 +- 0.04, V-R = 0.60 +- 0.02) with no variation in color between minimum and maximum light. The large light variation, relatively long double-peaked period and absence of rotational color change argue against explanations due to albedo markings or elongation due to high angular momentum. Instead, we suggest that 2001 QG298 may be a very close or contact binary similar in structure to what has been independently proposed for the Trojan asteroid 624 Hektor. If so, its rotational period would be twice the lightcurve period or 13.7744 +- 0.0004 hr. By correcting for the effects of projection, we estimate that the fraction of similar objects in the Kuiper Belt is at least 10% to 20% with the true fraction probably much higher. A high abundance of close and contact binaries is expected in some scenarios for the evolution of binary Kuiper Belt objects.Comment: 15 text pages,6 figures(Color),5 Tables, Accepted to AJ for May 200

Linear polarization of a group of symbiotic systems

We report linear polarization measurements of a set of symbiotic stars, made at several epochs during the period 1994-1998. Evidence of intrinsic polarization is looked for from the wavelength dependence of the polarization degree and position angle in UBVRI bands. The results have also been analysed to search for temporal variability of polarization. Several objects have shown a polarization spectrum different from that produced by interstellar dust grains and/or polarimetric variations on time scales as short as several days or months, indicating the presence of polarization component of circumstellar origin.Facultad de Ciencias Astronómicas y Geofísica

Linear polarization of a group of symbiotic systems

We report linear polarization measurements of a set of symbiotic stars, made at several epochs during the period 1994-1998. Evidence of intrinsic polarization is looked for from the wavelength dependence of the polarization degree and position angle in UBVRI bands. The results have also been analysed to search for temporal variability of polarization. Several objects have shown a polarization spectrum different from that produced by interstellar dust grains and/or polarimetric variations on time scales as short as several days or months, indicating the presence of polarization component of circumstellar origin.Facultad de Ciencias Astronómicas y Geofísica

Three cases of mcr-1-positive colistin-resistant Escherichia coli bloodstream infections in Italy, August 2016 to January 2017

We describe three cases of bloodstream infection caused by colistin-resistant Escherichia coli in patients in a tertiary hospital in Italy, between August 2016 and January 2017. Whole genome sequencing detected the mcr-1 gene in three isolated strains belonging to different sequence types (STs). This occurrence of three cases with mcr-1-positive E. coli belonging to different STs in six months suggests a widespread problem in settings where high multidrug resistance is endemic such as in Italy

Multiple Klebsiella pneumoniae KPC Clones Contribute to an Extended Hospital Outbreak

The circulation of carbapenem-resistant Klebsiella pneumoniae (CRKP) is a significant problem worldwide. In this work we characterize the isolates and reconstruct the spread of a multi-clone epidemic event that occurred in an Intensive Care Unit in a hospital in Northern Italy. The event took place from August 2015 to May 2016 and involved 23 patients. Twelve of these patients were colonized by CRKP at the gastrointestinal level, while the other 11 were infected in various body districts. We retrospectively collected data on the inpatients and characterized a subset of the CRKP isolates using antibiotic resistance profiling and whole genome sequencing. A SNP-based phylogenetic approach was used to depict the evolutionary context of the obtained genomes, showing that 26 of the 32 isolates belong to three genome clusters, while the remaining six were classified as sporadic. The first genome cluster was composed of multi-resistant isolates of sequence type (ST) 512. Among those, two were resistant to colistin, one of which indicating the insurgence of resistance during an infection. One patient hospitalized in this period was colonized by two strains of CRKP, both carrying the blaKPC gene (variant KPC-3). The analysis of the genome contig containing the blaKPC locus indicates that the gene was not transmitted between the two isolates. The second infection cluster comprised four other genomes of ST512, while the third one (ST258) colonized 12 patients, causing five clinical infections and resulting in seven deaths. This cluster presented the highest level of antibiotic resistance, including colistin resistance in all 17 analyzed isolates. The three outbreaking clones did not present more virulence genes than the sporadic isolates and had different patterns of antibiotic resistance, however, were clearly distinct from the sporadic ones in terms of infection status, being the only ones causing overt infections

ESMO recommendations on the standard methods to detect NTRK fusions in daily practice and clinical research

Abstract Background NTRK1, NTRK2 and NTRK3 fusions are present in a plethora of malignancies across different histologies. These fusions represent the most frequent mechanism of oncogenic activation of these receptor tyrosine kinases, and biomarkers for the use of TRK small molecule inhibitors. Given the varying frequency of NTRK1/2/3 fusions, crucial to the administration of NTRK inhibitors is the development of optimal approaches for the detection of human cancers harbouring activating NTRK1/2/3 fusion genes. Materials and methods Experts from several Institutions were recruited by the European Society for Medical Oncology (ESMO) Translational Research and Precision Medicine Working Group (TR and PM WG) to review the available methods for the detection of NTRK gene fusions, their potential applications, and strategies for the implementation of a rational approach for the detection of NTRK1/2/3 fusion genes in human malignancies. A consensus on the most reasonable strategy to adopt when screening for NTRK fusions in oncologic patients was sought, and further reviewed and approved by the ESMO TR and PM WG and the ESMO leadership. Results The main techniques employed for NTRK fusion gene detection include immunohistochemistry, fluorescence in situ hybridization (FISH), RT-PCR, and both RNA-based and DNA-based next generation sequencing (NGS). Each technique has advantages and limitations, and the choice of assays for screening and final diagnosis should also take into account the resources and clinical context. Conclusion In tumours where NTRK fusions are highly recurrent, FISH, RT-PCR or RNA-based sequencing panels can be used as confirmatory techniques, whereas in the scenario of testing an unselected population where NTRK1/2/3 fusions are uncommon, either front-line sequencing (preferentially RNA-sequencing) or screening by immunohistochemistry followed by sequencing of positive cases should be pursued

Multiband optical polarimetry of the BL Lac object PKS 2155--304. Intranight and long term variability

The polarized and total flux of the BL Lac object PKS 2155-304 were monitored intensively and simultaneously in the optical UBVRI bands with the Turin photopolarimeter at the CASLEO 2.15 m telescope during 4 campaigns in June, August, November 1998 and August 1999. The effective observation time amounted to ~47 hours. PKS 2155-304 showed a linear polarization percentage (P) usually ranging between 3% and 7% and a polarization position angle (PA) mainly between 70 and 120 deg. The highest temporal resolution of our observations, 15 minutes, is unprecedented for polarimetric monitoring of this source, and has allowed us to detect amplitude variations of the linear polarization percentage from 6 to 7.5% in time scales of hours. In some nights the polarization percentage seems to increase toward shorter wavelengths, however the polarized spectrum does not vary significantly with time. The most remarkable variability event occurred on 1998 June 18, when the degree of linear polarization decreased by more than a factor 2 in one day in all bands, while the PA rotated by 90 deg. This is consistent with the presence of two emission components, of different polarization degree and position angle. Intranight variability of P and PA can be interpreted with small amplitude physical or geometrical changes within the jet. Measurements of the circular polarization over time intervals of days set upper limits of 0.2%. Simultaneous photometry taken with the Turin Photopolarimeter and with a CCD camera at Cordoba Astronomical Observatory did not show light variations correlated with those of the linearly polarized flux.Comment: 21 pages, 6 eps figures. Accepted for The Astrophysical Journal Supplement Serie

Linear polarization of a group of symbiotic systems

We report linear polarization measurements of a set of symbiotic stars, made at several epochs during the period 1994-1998. Evidence of intrinsic polarization is looked for from the wavelength dependence of the polarization degree and position angle in UBVRI bands. The results have also been analysed to search for temporal variability of polarization. Several objects have shown a polarization spectrum different from that produced by interstellar dust grains and/or polarimetric variations on time scales as short as several days or months, indicating the presence of polarization component of circumstellar origin.Facultad de Ciencias Astronómicas y Geofísica