8 research outputs found
Innovative ready to use carrier-bacteria devices for bioremediation of oil contaminated water
Bioremediation, that uses microorganisms to remove environmental pollutants, is the best way of restoring
the environment due to its low cost and sustainability. Immobilization of microorganisms capable of
degrading specific contaminants significantly promotes bioremediation processes. An innovative ready to
use bioremediation system to clean up oil-contaminated water was developed immobilizing highly
performant marine and soil HC degrading bacteria, on biodegradable oil-absorbing carriers. Two soil
Actinobacteria (Gordonia sp. SoCg, Nocardia sp. SoB) and two marine Gammaproteobacteria (Alcanivorax
sp. SK2, Oleibacter sp.5), were immobilized on biopolymeric membranes prepared by electrospinning
(polylactic acid, PLA and polycaprolactone, PCL). These carriers are characterized by high uptake capacity,
oil retention, buoyancy, durability, reusability and recoverability of the oil absorbed. The morphology of the
carriers and microbial adhesion and proliferation were evaluated using scanning electron microscopy (SEM).
A high capacity of adhesion and proliferation of bacterial cells was observed on membranes after 5 days. The
bioremediation efficiency of the carrier-bacteria systems was tested on crude oil by GC-FID analysis and
compared whit planktonic cells. The bacterial immobilization on PLA and PCL membranes was a
promoting factor for biodegradation, increasing hydrocarbon removal up to 20%, in respect to planktonic
cells. Biofilm-mediated bioremediation is a versatile tool to be developed for in situ and ex situ
bioremediation of aquatic systems. Several applications can be designed to exploit both the high oil uptake
capacity of the carriers, and the biodegradation potential of autochtonous microrganisms and/or of selected
microorganisms that are immobilized on the carriers before exposure to the contaminated site
Innovative, ecofriendly biosorbent-biodegrading biofilms for bioremediation of oil- contaminated water
Immobilization of microorganisms capable of degrading specific contaminants significantly promotes bioremediation processes. In this study, innovative and ecofriendly biosorbent-biodegrading biofilms have been developed in order to remediate oil-contaminated water. This was achieved by immobilizing hydrocarbon-degrading gammaproteobacteria and actinobacteria on biodegradable oil-adsorbing carriers, based on polylactic acid and polycaprolactone electrospun membranes. High capacities for adhesion and proliferation of bacterial cells were observed by scanning electron microscopy. The bioremediation efficiency of the systems, tested on crude oil and quantified by gas chromatography, showed that immobilization increased hydrocarbon biodegradation by up to 23 % compared with free living bacteria. The resulting biosorbent biodegrading biofilms simultaneously adsorbed 100 % of spilled oil and biodegraded more than 66 % over 10 days, with limited environmental dispersion of cells. Biofilm-mediated bioremediation, using eco-friendly supports, is a low-cost, low-impact, versatile tool for bioremediation of aquatic systems
Acceptability of the transitional wearable companion â+meâ in typical children: a pilot study
This work presents the results of the first experimentation of +me-the first prototype of
Transitional Wearable Companionârun on 15 typically developed (TD) children with ages
between 8 and 34 months. +me is an interactive device that looks like a teddy bear that
can be worn around the neck, has touch sensors, can emit appealing lights and sounds,
and has input-output contingencies that can be regulated with a tablet via Bluetooth.
The participants were engaged in social play activities involving both the device and
an adult experimenter. +me was designed with the objective of exploiting its intrinsic
allure as an attractive toy to stimulate social interactions (e.g., eye contact, turn taking,
imitation, social smiles), an aspect potentially helpful in the therapy of Autism Spectrum
Disorders (ASD) and other Pervasive Developmental Disorders (PDD). The main purpose
of this preliminary study is to evaluate the general acceptability of the toy by TD children,
observing the elicited behaviors in preparation for future experiments involving children
with ASD and other PDD. First observations, based on video recording and scoring,
show that +me stimulates good social engagement in TD children, especially when their
age is higher than 24 months
Quimioterapia super-seletiva da artĂ©ria oftĂĄlmica em retinoblastoma intraocular : acrĂ©scimo terapĂȘutico no HCPA para evitar a enucleação
BIODEGRADING BIOFILMS ON INNOVATIVE BIOPOLYMERIC SUPPORTS
ABSTRACT
Water bioremediation is traditionally carried out using â free â bacterial cells, however, in recent years, utilization of âimmobilizedâ bacterial cells on adsorbing matrices, has gained attention as a promising technique due to biotechnological and economic benefits (Sonawane et al., 2022). Bacterial biofilms show greater resilience, survival and degradative activity for longer periods than cells in the planktonic state (Alessandrello et al., 2017); moreover immobilization reduces bioremediation costs, eliminate cell dilution and dispersion in the environment (Bayat et al., 2015). Possible applications of immobilized biodegrading bacteria require long-term survival and maintenance of biodegrading performances. In this study, combinations of polylactic acid (PLA) and polycaprolactone (PCL) biodegradable membrane carriers hosting selected HC-biodegrading marine and soil bacterial biofilms were tested after different incubation periods and their survival was monitored over time, simulating storage effects.
Results
Soil hydrocarbon (HC) degrading actinobacteria and marine hydrocarbonoclastic bacteria were immobilized on absorbent biodegradable biopolymeric polylactic acid (PLA) and polycaprolactone (PCL) membranes (Scaffaro et al., 2017, Catania et al., 2020). Combinations of HC-degrading bacteria and biopolymers were obtained and tested on hexadecane. After 5, 10 and 15 days incubation, the capacity of adhesion and proliferation of bacterial cells into the biopolymers was verified by scanning electron microscopy (SEM); PLA and PCL nanofibers were covered by bacterial cells already after 5 days incubation; Total biomass (estimated as total dsDNA) extracted from biofilms confirmed the colonization up to 15 days incubation. Viable plate counts showed that survival of the bacterial strains was high for the entire experimental period. HC biodegradation ability of biofilms was assessed by high resolution GC-FID analysis, after extraction of total residual HC from the liquid medium and from biopolymers, incubated for different times. HC degradation was observed during the whole experiment and resulted higher in respect to the free-living bacterial cultures. Survival tests of bacterial biofilms adsorbed on biopolymers for up to 30 days are in progress.
Conclusions
The synergistic exploitation of the high absorbent capacity of biodegradable nanofiber membranes and the catabolic capacity of HC-degrading bacteria allow to obtain biodegrading biofilms endowed with higher removal capacity of hexadecane in respect to free-living bacterial cultures. The survival and biodegrading performances of the biofilm-carrier systems is maintained after 30 days incubation. A green, low-cost, biodegradable and reusable bioremediation tool is obtained without negative impacts on the environment.
References:
Alessandrello, M. J., TomĂĄs, M. S. J., Raimondo, E. E., Vullo, D. L. and Ferrero, M. A. âPetroleum oil removal by immobilized bacterial cells on polyurethane foam under different temperature conditionsâ, Marine pollution bulletin, 122(1-2), 156-160 (2017).
Bayat, Z., Hassanshahian, M. and Cappello, S. âImmobilization of microbes for bioremediation of crude oil polluted environments: a mini reviewâ, The open microbiology journal, 9, 48 (2015).
Catania, V., Lopresti, F., Cappello, S., Scaffaro, R. and Quatrini, P. âInnovative, ecofriendly biosorbent-biodegrading biofilms for bioremediation of oil-contaminated waterâ, New Biotechnology, 58, 25-31 (2020).
Scaffaro, R., Lopresti, F., Catania, V., Santisi, S., Cappello, S., Botta, L. and Quatrini, P. âPolycaprolactone-based scaffold for oil-selective sorption and improvement of bacteria activity for bioremediation of polluted water: Porous PCL system obtained by leaching melt mixed PCL/PEG/NaCl composites: Oil uptake performance and bioremediation efficiencyâ, European Polymer Journal, 91, 260-273 (2017).
Sonawane, J. M., Rai, A. K., Sharma, M., Tripathi, M. and Prasad, R. âMicrobial biofilms: Recent advances and progress in environmental bioremediationâ, Science of The Total Environment, 153843 (2022).
Catania, V., Santisi, S., Signa, G., Vizzini, S., Mazzola, A., Cappello, S., ... & Quatrini, P. (2015). Intrinsic bioremediation potential of a chronically polluted marine coastal area. Marine Pollution Bulletin, 99(1-2), 138-149.
Lo Piccolo, L., De Pasquale, C., Fodale, R., Puglia, A. M., & Quatrini, P. (2011). Involvement of an alkane hydroxylase system of Gordonia sp. strain SoCg in degradation of solid n-alkanes. Applied and environmental microbiology, 77(4), 1204-1213.
Quatrini, P., Scaglione, G., De Pasquale, C., Riela, S., & Puglia, A. M. (2008). Isolation of Gramâpositive nâalkane degraders from a hydrocarbonâcontaminated Mediterranean shoreline. Journal of applied microbiology, 104(1), 251-259
Acceptability of Transitional Wearable Companion "+me" in typical children: a pilot study
This work presents the results of the first experimentation of +me-the first prototype of
Transitional Wearable Companion\u2013run on 15 typically developed (TD) children with ages
between 8 and 34 months. +me is an interactive device that looks like a teddy bear that
can be worn around the neck, has touch sensors, can emit appealing lights and sounds,
and has input-output contingencies that can be regulated with a tablet via Bluetooth.
The participants were engaged in social play activities involving both the device and
an adult experimenter. +me was designed with the objective of exploiting its intrinsic
allure as an attractive toy to stimulate social interactions (e.g., eye contact, turn taking,
imitation, social smiles), an aspect potentially helpful in the therapy of Autism Spectrum
Disorders (ASD) and other Pervasive Developmental Disorders (PDD). The main purpose
of this preliminary study is to evaluate the general acceptability of the toy by TD children,
observing the elicited behaviors in preparation for future experiments involving children
with ASD and other PDD. First observations, based on video recording and scoring,
show that +me stimulates good social engagement in TD children, especially when their
age is higher than 24 months