Transfer of Fas (CD95) protein from the cell surface to the surface of polystyrene beads coated with anti-Fas antibody clone CH-11

Mouse monoclonal anti-Fas (CD95) antibody clone CH-11 has been widely used in research on apoptosis. CH-11 has the ability to bind to Fas protein on cell surface and induce apoptosis. Here, we used polystyrene beads coated with CH-11 to investigate the role of lipid rafts in Fas-mediated apoptosis in SKW6.4 cells. Unexpectedly, by treatment of the cells with CH-11-coated beads Fas protein was detached from cell surface and transferred to the surface of CH-11-coated beads. Western blot analysis showed that Fas protein containing both extracellular and intracellular domains was attached to the beads. Fas protein was not transferred from the cells to the surface of the beads coated with other anti-Fas antibodies or Fas ligand. Similar phenomenon was observed in Jurkat T cells. Furthermore, CH-11-induced apoptosis was suppressed by pretreatment with CH-11-coated beads in Jurkat cells. These results suggest that CH-11 might possess distinct properties on Fas protein compared with other anti-Fas antibodies or Fas ligand, and also suggest that caution should be needed to use polystyrene beads coated with antibodies such as CH-11

Dynamic behaviors of dust particles in the plasma-sheath boundary

A variety of dynamic behaviors in dusty plasmas is expected under the experimental condition of weak friction with gas molecules. The device "KAGEROU" provides such an environment for dynamic collective phenomena. Self-excited dust oscillations in Coulomb crystals have been observed at low values of plasma density and gas pressure. An instability mechanism was identified to be delayed charging in an inhomogeneous equilibrium dust charge in the sheath. The theoretical growth rate was formulated in relation to the destabilization of a transverse dust lattice wave (T-DLW), which was found to be very sensitive to the presence of a small amount of hot electrons which produces a substantial positive equilibrium charge gradient ∇Qd-eq around the equilibrium position of dust particles in the plasma-sheath boundary. The first experimental observation of a correlated self-excited vertical oscillations in a one-dimensional dust chain indicates a destabilization of T-DLW. The experimental condition is very consistent with the parameter area which predicts numerically an instability of T-DLW

Discerning the origins of the Negritos, first Sundaland people: Deep divergence and archaic admixture

Human presence in Southeast Asia dates back to at least 40,000 years ago, when the current islands formed a continental shelf called Sundaland. In the Philippine Islands, Peninsular Malaysia, and Andaman Islands, there exist indigenous groups collectively called Negritos whose ancestry can be traced to the “First Sundaland People.” To understand the relationship between these Negrito groups and their demographic histories, we generated genome-wide single nucleotide polymorphism data in the Philippine Negritos and compared them with existing data from other populations. Phylogenetic tree analyses show that Negritos are basal to other East and Southeast Asians, and that they diverged from West Eurasians at least 38,000 years ago. We also found relatively high traces of Denisovan admixture in the Philippine Negritos, but not in the Malaysian and Andamanese groups, suggesting independent introgression and/or parallel losses involving Denisovan introgressed regions. Shared genetic loci between all three Negrito groups could be related to skin pigmentation, height, facial morphology and malarial resistance. These results show the unique status of Negrito groups as descended from the First Sundaland People

White dwarf spins from low mass stellar evolution models

The prediction of the spins of the compact remnants is a fundamental goal of the theory of stellar evolution. Here, we confront the predictions for white dwarf spins from evolutionary models including rotation with observational constraints. We perform stellar evolution calculations for stars in the mass range 1... 3\mso, including the physics of rotation, from the zero age main sequence into the TP-AGB stage. We calculate two sets of model sequences, with and without inclusion of magnetic fields. From the final computed models of each sequence, we deduce the angular momenta and rotational velocities of the emerging white dwarfs. While models including magnetic torques predict white dwarf rotational velocities between 2 and 10 km s$^{-1}$, those from the non-magnetic sequences are found to be one to two orders of magnitude larger, well above empirical upper limits. We find the situation analogous to that in the neutron star progenitor mass range, and conclude that magnetic torques may be required in order to understand the slow rotation of compact stellar remnants in general.Comment: Accepted for A&A Letter

Neutrino mean free paths in spin-polarized neutron Fermi liquids

Neutrino mean free paths in magnetized neutron matter are calculated using the Hartree-Fock approximation with effective Skyrme and Gogny forces in the framework of the Landau Fermi Liquid Theory. It is shown that describing nuclear interaction with Skyrme forces and for magnetic field strengths $log_{10} B(G) \gtrsim 17$, the neutrino mean free paths stay almost unchanged at intermediate densities but they largely increase at high densities when they are compared to the field-free case results. However the description with Gogny forces differs from the previous and mean free paths stay almonst unchanged or decrease at densities $[1-2]\rho_0$. This different behaviour can be explained due to the combination of common mild variation of the Landau parameters with both types of forces and the values of the nucleon effective mass and induced magnetization of matter under presence of a strong magnetic field as described with the two parametrizations of the nuclear interaction.Comment: 9 pages, 3 figure

Zinc oxide nanoparticle-coated films: fabrication, characterization, and antibacterial properties

In this article, novel antibacterial PVC-based films coated with ZnO nanoparticles (NPs) were fabricated, characterized, and studied for their antibacterial properties. It was shown that the ZnO NPs were coated on the surface of the PVC films uniformly and that the coating process did not affect the size and shape of the NPs on the surface of PVC films. Films coated with concentrations of either 0.2 or 0.075 g/L of ZnO NPs exhibited antibacterial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, but exhibited no antifungal activity against Aspergillus flavus and Penicillium citrinum. Smaller particles (100 nm) exhibited more potent antibacterial activity than larger particles (1000 nm). All ZnO-coated films maintained antibacterial activity after 30 days in water

Source parameters of the great Sumatran megathrust earthquakes of 1797 and 1833 inferred from coral microatolls

Large uplifts and tilts occurred on the Sumatran outer arc islands between 0.5° and 3.3°S during great historical earthquakes in 1797 and 1833, as judged from relative sea level changes recorded by annually banded coral heads. Coral data for these two earthquakes are most complete along a 160-km length of the Mentawai islands between 3.2° and 2°S. Uplift there was as great as 0.8 m in 1797 and 2.8 m in 1833. Uplift in 1797 extended 370 km, between 3.2° and 0.5°S. The pattern and magnitude of uplift imply megathrust ruptures corresponding to moment magnitudes (M_w) in the range 8.5 to 8.7. The region of uplift in 1833 ranges from 2° to at least 3.2°S and, judging from historical reports of shaking and tsunamis, perhaps as far as 5°S. The patterns and magnitude of uplift and tilt in 1833 are similar to those experienced farther north, between 0.5° and 3°N, during the giant Nias-Simeulue megathrust earthquake of 2005; the outer arc islands rose as much as 3 m and tilted toward the mainland. Elastic dislocation forward modeling of the coral data yields megathrust ruptures with moment magnitudes ranging from 8.6 to 8.9. Sparse accounts at Padang, along the mainland west coast at latitude 1°S, imply tsunami runups of at least 5 m in 1797 and 3–4 m in 1833. Tsunamis simulated from the pattern of coral uplift are roughly consistent with these reports. The tsunami modeling further indicates that the Indian Ocean tsunamis of both 1797 and 1833, unlike that of 2004, were directed mainly south of the Indian subcontinent. Between about 0.7° and 2.1°S, the lack of vintage 1797 and 1833 coral heads in the intertidal zone demonstrates that interseismic submergence has now nearly equals coseismic emergence that accompanied those earthquakes. The interseismic strains accumulated along this reach of the megathrust have thus approached or exceeded the levels relieved in 1797 and 1833