Transfer of Fas (CD95) protein from the cell surface to the surface of polystyrene beads coated with anti-Fas antibody clone CH-11

Mouse monoclonal anti-Fas (CD95) antibody clone CH-11 has been widely used in research on apoptosis. CH-11 has the ability to bind to Fas protein on cell surface and induce apoptosis. Here, we used polystyrene beads coated with CH-11 to investigate the role of lipid rafts in Fas-mediated apoptosis in SKW6.4 cells. Unexpectedly, by treatment of the cells with CH-11-coated beads Fas protein was detached from cell surface and transferred to the surface of CH-11-coated beads. Western blot analysis showed that Fas protein containing both extracellular and intracellular domains was attached to the beads. Fas protein was not transferred from the cells to the surface of the beads coated with other anti-Fas antibodies or Fas ligand. Similar phenomenon was observed in Jurkat T cells. Furthermore, CH-11-induced apoptosis was suppressed by pretreatment with CH-11-coated beads in Jurkat cells. These results suggest that CH-11 might possess distinct properties on Fas protein compared with other anti-Fas antibodies or Fas ligand, and also suggest that caution should be needed to use polystyrene beads coated with antibodies such as CH-11

The Core-Collapse Supernova with "Non-Uniform" Magnetic Fields

We perform two-dimensional numerical simulations on the core-collapse of a massive star with strong magnetic fields and differential rotations using a numerical code ZEUS-2D. Changing field configurations and laws of differential rotation parametrically, we compute 14 models and investigate effects of these parameters on the dynamics. In our models, we do not solve the neutrino transport and instead employ a phenomenological parametric EOS that takes into account the neutrino emissions. As a result of the calculations, we find that the field configuration plays a significant role in the dynamics of the core if the initial magnetic field is large enough. Models with initially concentrated fields produce more energetic explosions and more prolate shock waves than the uniform field. Quadrapole-like fields produce remarkably collimated and fast jet, which might be important for gamma-ray bursts(GRB). The Lorentz forces exerted in the region where the plasma-beta is less than unity are responsible for these dynamics. The pure toroidal field, on the other hand, does not lead to any explosion or matter ejection. This suggests the presupernova models of Heger et al.(2003), in which toroidal fields are predominant, is disadvantageous for the magnetorotation-induced supernova considered here. Models with initially weak magnetic fields do not lead to explosion or matter ejection, either. In these models magnetic fields play no role as they do not grow on the timescale considered in this paper so that the magnetic pressure could be comparable to the matter pressure. This is because the exponential field growth as expected in MRI is not seen in our models. The magnetic field is amplified mainly by field-compression and field-wrapping in our simulations.Comment: 24 pages, 5 figures, ApJ in press, typos correcte

The Intermediate r-process in Core-collapse Supernovae Driven by the Magneto-rotational Instability

We investigated r-process nucleosynthesis in magneto-rotational supernovae, based on a new explosion mechanism induced by the magneto-rotational instability (MRI). A series of axisymmetric magneto-hydrodynamical simulations with detailed microphysics including neutrino heating is performed, numerically resolving the MRI. Neutrino-heating dominated explosions, enhanced by magnetic fields, showed mildly neutronrich ejecta producing nuclei up to A similar to 130 (i. e., the weak r-process), while explosion models with stronger magnetic fields reproduce a solar-like r-process pattern. More commonly seen abundance patterns in our models are in between the weak and regular r-process, producing lighter and intermediate-mass nuclei. These intermediate r-processes exhibit a variety of abundance distributions, compatible with several abundance patterns in r-process-enhanced metal-poor stars. The amount of Eu ejecta similar to 10(-5) M circle dot in magnetically driven jets agrees with predicted values in the chemical evolution of early galaxies. In contrast, neutrino-heating dominated explosions have a significant amount of Fe (Ni-56) and Zn, comparable to regular supernovae and hypernovae, respectively. These results indicate magneto-rotational supernovae can produce a wide range of heavy nuclei from iron-group to r-process elements, depending on the explosion dynamics

Dynamic behaviors of dust particles in the plasma-sheath boundary

A variety of dynamic behaviors in dusty plasmas is expected under the experimental condition of weak friction with gas molecules. The device "KAGEROU" provides such an environment for dynamic collective phenomena. Self-excited dust oscillations in Coulomb crystals have been observed at low values of plasma density and gas pressure. An instability mechanism was identified to be delayed charging in an inhomogeneous equilibrium dust charge in the sheath. The theoretical growth rate was formulated in relation to the destabilization of a transverse dust lattice wave (T-DLW), which was found to be very sensitive to the presence of a small amount of hot electrons which produces a substantial positive equilibrium charge gradient ∇Qd-eq around the equilibrium position of dust particles in the plasma-sheath boundary. The first experimental observation of a correlated self-excited vertical oscillations in a one-dimensional dust chain indicates a destabilization of T-DLW. The experimental condition is very consistent with the parameter area which predicts numerically an instability of T-DLW

Ehrenfest relations and magnetoelastic effects in field-induced ordered phases

Magnetoelastic properties in field-induced magnetic ordered phases are studied theoretically based on a Ginzburg-Landau theory. A critical field for the field-induced ordered phase is obtained as a function of temperature and pressure, which determine the phase diagram. It is found that magnetic field dependence of elastic constant decreases discontinuously at the critical field, Hc, and that it decreases linearly with field in the ordered phase (H>Hc). We found an Ehrenfest relation between the field dependence of the elastic constant and the pressure dependence of critical field. Our theory provides the theoretical form for magnetoelastic properties in field- and pressure-induced ordered phases.Comment: 7 pages, 3 figure

Discerning the origins of the Negritos, first Sundaland people: Deep divergence and archaic admixture

Human presence in Southeast Asia dates back to at least 40,000 years ago, when the current islands formed a continental shelf called Sundaland. In the Philippine Islands, Peninsular Malaysia, and Andaman Islands, there exist indigenous groups collectively called Negritos whose ancestry can be traced to the “First Sundaland People.” To understand the relationship between these Negrito groups and their demographic histories, we generated genome-wide single nucleotide polymorphism data in the Philippine Negritos and compared them with existing data from other populations. Phylogenetic tree analyses show that Negritos are basal to other East and Southeast Asians, and that they diverged from West Eurasians at least 38,000 years ago. We also found relatively high traces of Denisovan admixture in the Philippine Negritos, but not in the Malaysian and Andamanese groups, suggesting independent introgression and/or parallel losses involving Denisovan introgressed regions. Shared genetic loci between all three Negrito groups could be related to skin pigmentation, height, facial morphology and malarial resistance. These results show the unique status of Negrito groups as descended from the First Sundaland People

White dwarf spins from low mass stellar evolution models

The prediction of the spins of the compact remnants is a fundamental goal of the theory of stellar evolution. Here, we confront the predictions for white dwarf spins from evolutionary models including rotation with observational constraints. We perform stellar evolution calculations for stars in the mass range 1... 3\mso, including the physics of rotation, from the zero age main sequence into the TP-AGB stage. We calculate two sets of model sequences, with and without inclusion of magnetic fields. From the final computed models of each sequence, we deduce the angular momenta and rotational velocities of the emerging white dwarfs. While models including magnetic torques predict white dwarf rotational velocities between 2 and 10 km s$^{-1}$, those from the non-magnetic sequences are found to be one to two orders of magnitude larger, well above empirical upper limits. We find the situation analogous to that in the neutron star progenitor mass range, and conclude that magnetic torques may be required in order to understand the slow rotation of compact stellar remnants in general.Comment: Accepted for A&A Letter