Force transmission in migrating cells

Analysis of the relationship between actin network velocity and traction forces at the substrate shows that force transmission mechanisms vary with distinct regions of the cell

Ergonomics and sustainability: Towards and embrace of complexity and emergence

Technology offers a promising route to a sustainable future, and ergonomics can serve a vital role. The argument of this article is that the lasting success of sustainability initiatives in ergonomics hinges on an examination of ergonomics' own epistemology and ethics. The epistemology of ergonomics is fundamentally empiricist and positivist. This places practical constraints on its ability to address important issues such as sustainability, emergence and complexity. The implicit ethical position of ergonomics is one of neutrality, and its positivist epistemology generally puts value-laden questions outside the parameters of what it sees as scientific practice. We argue, by contrast, that a discipline that deals with both technology and human beings cannot avoid engaging with questions of complexity and emergence and seeking innovative ways of addressing these issues.No Full Tex

An emerging clone, KPC-2-producing Klebsiella pneumoniae ST16, associated with high mortality rates in a CC258 endemic setting

Background Carbapenemase-producing K. pneumoniae have become a global priority, not least in low-middle income countries. Here, we report the emergence and clinical impact of a novel KPC-K. pneumoniae ST16 clone in a Clonal Complex (CC)258 endemic setting. Methods In a teaching Brazilian hospital, a retrospective cohort of adult KPC-KP bloodstream infections (BSI) cases (January 2014 to December 2016) was established to study the molecular epidemiology and its impact on outcome (30-day all-cause mortality). KPC-KP isolates were MLST-typed. Survival analysis between ST/CC groups and risk factors for fatal outcome (logistic regression) were evaluated. Representative isolates underwent whole genome sequencing (WGS), and had their virulence tested in a Galleria larvae model. Results One hundred sixty-five unique KPC-KP BSI cases were identified. CC258 was predominant (66%), followed by ST16 (12%). The overall 30-day mortality rate was 60%; in contrast, 95% of ST16 cases were fatal. Patient’s severity scores were high and baseline clinical variables were not statistically different across ST’s. In multivariate analysis, ST16 (OR 21.4; CI95% 2.3-202.8; p=0,008) and septic shock (OR 11.9; CI95% 4.2-34.1; p<0,001) were independent risk factors for fatal outcome. ST16 clone carried up to 14 resistance genes, including blaKPC-2 in an IncFIBpQIL plasmid, KL51 capsule and Yersiniabactin virulence determinants. ST16 clone was highly pathogenic in the larvae model. Conclusions Mortality rates were high in this KPC-KP BSI cohort, where CC258 is endemic. An emerging ST16 clone was associated with high mortality. Our results suggest that even in endemic settings, highly virulent clones can rapidly emerge demanding constant monitoring

Using a System Maturity Scale to Monitor and Evaluate the Development of Systems

Proceedings Paper (for Acquisition Research Program)To address integration, the Systems Development & Maturity Laboratory (SD&ML) at Stevens Institute of Technology introduced another prescriptive metric called Integration Readiness Level (IRL). Combining TRL and IRL scales, SD&ML has formulated a System Readiness Level (SRL). SRL is an aggregate measure that characterizes the progress that has been accomplished by a system under development based on the observable readiness characteristics of the technology and integration elements, not the cost and schedule values. This paper describes the application of SRL to a constrained resource optimization model to determine an optimal development plan that identifies which technologies and integration elements should be matured to which levels such that a specific level of system readiness is achieved by a certain time. This optimal plan can be used to monitor and evaluate the actual progress of the system''it can be the basis of a systems lifecycle maturity management approach called System Earned Readiness Management (SERM). A simple example is used to illustrate SERM.Naval Postgraduate School Acquisition Research ProgramApproved for public release; distribution is unlimited

System Maturity Indices for Decision Support in the Defense Acquisition Process

Proceedings Paper (for Acquisition Research Program)The Technology Readiness Level (TRL) scale is a measure of maturity of an individual technology, with a view towards operational use in a system context. A comprehensive set of concerns becomes relevant when this metric is abstracted from an individual technology to a system context, which may involve interplay among multiple technologies that are integrated through the defense acquisition process. This paper proposes the development of a system-focused approach for managing system development and making effective and efficient decisions during the defense acquisition process. For this to be accomplished, a new System Readiness Level (SRL) index will incorporate both the current TRL scale and the concept of an integration readiness level (IRL). This paper describes the foundations for the SRL and provides techniques for determining current and future readiness of a system to determine its position in the defense acquisition process. In addition, it proposes optimization models than can provide management with an optimal development plan that can meet the objectives of the development team, based on constrained resources. These, in turn, can become the foundation for the development of a monitoring and evaluation tool that will be analogous to Earned Value Management used in project management.Naval Postgraduate School Acquisition Research ProgramApproved for public release; distribution is unlimited

Paradox: Holarchical view of system of systems engineering management

This paper focuses on a method of developing a framework for effective System of Systems Engineering (SoSE) management.While developing the management process in dealing with SoSE, we are searching for ways to engineer multiple integrated complex systems, which can be concurrently, highly emergent and effectively governable. The authors propose to utilize the "holarchical view" methodology to identify and examine this essential phenomenon of paradoxes of SoSE management. The proposed method is based on the Boardman-Sauser five distinguishing characteristics of Autonomy, Belonging, Connectivty, Diversity, and Emergence. The outcome of this research would influence further studies on the topic and greatly contribute to the SoSE body of knowledge.Alex Gorod, Brian Sauser, John Boardma

The Body of Knowledge and Curriculum to Advance Systems Engineering

Much of the funding and sponsorship for BKCASE was provided by the U.S. Department of Defense