Neural regulation in tooth regeneration of Ambystoma mexicanum

The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nerve-dependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration. The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nervedependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration

Ultrafiltration attenuates cardiopulmonary bypass–induced acute lung injury in a canine model of single-lung transplantation

ObjectiveThe purpose of this study was to investigate the effects of cardiopulmonary bypass and ultrafiltration on graft function in a canine single-lung transplantation model.MethodsFifteen left single-lung transplantations were done in weight-mismatched canine pairs. The animals were divided into 3 groups: group 1, in which transplantation was done without cardiopulmonary bypass; group 2, in which transplantation was done with cardiopulmonary bypass and in which the cardiopulmonary bypass flow was decreased slowly with controlled pulmonary artery pressure; and group 3, in which transplantation was done with cardiopulmonary bypass and ultrafiltration. Hemodynamic parameters and lung function were monitored for 6 hours after reperfusion. The grafts were harvested for histologic studies, myeloperoxidase assay, and real-time quantitive reverse transcription–polymerase chain reaction of mRNA encoding interleukin 6.ResultsThe hemodynamic parameters were similar among the 3 groups. In group 1 Pao2 and alveolar to arterial gradient for O2 levels were excellent throughout the 6-hour observation period, but in group 2 they progressively deteriorated. However, ultrafiltration significantly (P = .02) improved the Pao2 level in group 3. On histology, interstitial edema and polynuclear cell infiltration were most marked in group 2 and significantly worse than in groups 1 and 3. Myeloperoxidase assay and real-time quantitative reverse transcription–polymerase chain reaction showed increased myeloperoxidase activity and interleukin 6 gene expression in group 2 grafts compared with group 1 grafts. Myeloperoxidase activity and interleukin 6 gene expression were suppressed with ultrafiltration.ConclusionsCardiopulmonary bypass had negative effects on the graft, but ultrafiltration attenuated acute lung dysfunction by reducing the inflammatory response

Monitoring and evaluation of simulated underground coal gasification in an ex-situ experimental artificial coal seam system

In this study, to better simulate underground coal gasification (UCG), an artificial coal seam was constructed to use as a simulated underground gasifier, which comprised coal blocks excavated from the coal seam. This study reports the process and results of three independently designed experiments using coaxial-hole and linking-hole UCG models: (a) a coaxial model using a coaxial pipeline as a gasification channel, (b) a coaxial model using the coaxial pipeline combined with a bottom cross-hole, and (c) a linking-hole model using a horizontal V-shaped cross-hole. In the present work, the fracturing activities and cavity growth inside the reactor were monitored with acoustic emission (AE) technologies. During the process, the temperature profiles, gas production rate, and gas content were measured successively. The results show that AE activities monitored during UCG process are significantly affected by operational variables such as feed gas rate, feed gas content, and linking-hole types. Moreover, the amount of coal consumed during UCG process were estimated using both of the stoichiometric approach and balance computation of carbon (C) based on the product gas contents. A maximum error of less than 10% was observed in these methods, in which the gas leakage was also considered. This demonstrates that the estimated results using the proposed stoichiometric approach could be useful for evaluating energy recovery during UCG

The ADAXIALIZED LEAF1 gene functions in leaf and embryonic pattern formation in rice

AbstractThe adaxial–abaxial axis in leaf primordia is thought to be established first and is necessary for the expansion of the leaf lamina along the mediolateral axis. To understand axis information in leaf development, we isolated the adaxialized leaf1 (adl1) mutant in rice, which forms abaxially rolled leaves. adl1 leaves are covered with bulliform-like cells, which are normally distributed only on the adaxial surface. An adl1 double mutant with the adaxially snowy leaf mutant, which has albino cells that specifically appear in the abaxial mesophyll tissue, indicated that adl1 leaves show adaxialization in both epidermal and mesophyll tissues. The expression of HD-ZIPIII genes in adl1 mutant increased in mature leaves, but not in the young primordia or the SAM. This indicated that ADL1 may not be directly involved in determining initial leaf polarity, but rather is associated with the maintenance of axis information. ADL1 encodes a plant-specific calpain-like cysteine proteinase orthologous to maize DEFECTIVE KERNEL1. Furthermore, we identified intermediate and strong alleles of the adl1 mutant that generate shootless embryos and globular-arrested embryos with aleurone layer loss, respectively. We propose that ADL1 plays an important role in pattern formation of the leaf and embryo by promoting proper epidermal development

Hybrid Water Pump

This project seeks to use multiple sources to power a water pump. We will design a system which will combine inputs from solar and wind energy production to ensure the pump will have enough power to run. This will create a system powered by clean energy and will be self-sustained once completed. This will theoretically create a more sustainable source of power for the pump than only one of the sources. Because the power sources don’t require outside inputs (aside from sun and wind), the pump can be placed in a remote area. This could be very helpful in developing communities, where residents may not have a reliable power supply. This system will allow these types of communities to pump water from wells to bring back to their homes. This will also reduce dependence on non-renewable sources of energy. The system will consist of a solar panel, wind turbine, control system to integrate the sources, and the water pump. The system will be made to require minimal user input to run. Inside the control system, two DC-DC buck converters are used to regulate input voltages. Two diodes are used to joint 2 input sources and prevent backflow of energy. This method is selected instead of MISO (Multiple Inputs Single Output) converter due to its simplicity. An Arduino Uno microcontroller powered by AtMega328p microprocessor is used for displaying power reading from the two sources and the load. The microcontroller also control the relay for protection purposes. An adjustable speed drive and motors combination is studied and used for simulating the wind turbine generation. Relays and circuit breakers are also studied for circuit protection in renewable power systems. This project showcase the possibility of having a hybrid renewable system for common electrical appliances as well as pointing out some difficulties in such system

Bone marrow stroma cells are susceptible to prion infection.

Abnormal protease-resistant prion protein (PrP-res) is the only surrogate biochemical marker for prion diseases, and a sensitive technique to detect PrP-res in blood or tissues is urgently needed. Primary cultured bone marrow stromal cells (MSCs) expressed PrP and were capable of supporting stable human prion infection. Using a mouse-adapted BSE strain, we demonstrated that PrP-res can be detected in expanded MSCs. We then analyzed the bone marrow cells collected at autopsy from two individuals with sporadic Creutzfeldt-Jakob disease (CJD), and, in both cases, cultured MSCs were positive for PrP-res. These data would suggest that ex vivo MSC expansion accompanied by PrP-res analysis could be a helpful tool in the definitive diagnosis of prion disease at an earlier stage in the disease process than is currently possible, and with considerably less distress to the patient

Electrophoretic variants of blood proteins in Japanese VI. Transferrin

A multiplicity of transferrin variants have been detected in the course of the biochemical aspect of the study of the genetic effects of atomic bombs. Variants obtained from the studies of 19,770 individuals in Hiroshima and Nagasaki were compared by polyacrylamide slab gel electrophoresis using three kinds of buffer systems with different pH values and thin layer polyacrylamide gel isoelectric focusing. The variants were compared on the basis of their relative mobilities and isoelectric points; seven types of fast-moving variant (B-variant) and nine types of slow-moving variant (D-variant) were detected, involving a total of 154 and 273 individuals, respectively. All the variants were identified as genetic variants by family studies. No variant differend inaallele frequency between the two cities. The variants detected in this study were compared with variants detected in residents of Mie district (another Japanese population), Caucasoids, American blacks, and Amerindians. Six additional types of B-variant and four additional types of D-variant, which had not been detected in Hiroshima and Nagasaki, were identified.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/41593/1/10038_2005_Article_BF01876469.pd