Simulation of TI-SEPIC Converter for BLDC Motor Drives

Power Factor Correction (PFC) is one of the research areas in the field of power electronics due to the enormous power required in various industrial applications. In this work, a SEPIC converter with the Tapped Inductor model which is operated in Discontinuous current Conduction Mode (TI-SEPIC- DCM) is proposed for a BLDC Drive. The proposed TI-SEPIC-DCM also improves the voltage gain with the help of voltage multiplier cell and charge pump circuit. It also helps in attaining the ZVS and ZCS, which results in higher switching frequency range and reduction in size reduction. Moreover, a third order harmonic reduction control loop is also proposed to attain a better third order harmonic elimination. The proposed work has been simulated using MATLAB simulink and the results are also validated

Assembly and architecture of the EBV B cell entry triggering complex.

Epstein-Barr Virus (EBV) is an enveloped double-stranded DNA virus of the gammaherpesvirinae sub-family that predominantly infects humans through epithelial cells and B cells. Three EBV glycoproteins, gH, gL and gp42, form a complex that targets EBV infection of B cells. Human leukocyte antigen (HLA) class II molecules expressed on B cells serve as the receptor for gp42, triggering membrane fusion and virus entry. The mechanistic role of gHgL in herpesvirus entry has been largely unresolved, but it is thought to regulate the activation of the virally-encoded gB protein, which acts as the primary fusogen. Here we study the assembly and function of the reconstituted B cell entry complex comprised of gHgL, gp42 and HLA class II. The structure from negative-stain electron microscopy provides a detailed snapshot of an intermediate state in EBV entry and highlights the potential for the triggering complex to bring the two membrane bilayers into proximity. Furthermore, gHgL interacts with a previously identified, functionally important hydrophobic pocket on gp42, defining the overall architecture of the complex and playing a critical role in membrane fusion activation. We propose a macroscopic model of the initiating events in EBV B cell fusion centered on the formation of the triggering complex in the context of both viral and host membranes. This model suggests how the triggering complex may bridge the two membrane bilayers, orienting critical regions of the N- and C- terminal ends of gHgL to promote the activation of gB and efficient membrane fusion

Charge Detection Mass Spectrometry Reveals Conformational Heterogeneity in Megadalton-Sized Monoclonal Antibody Aggregates

Aggregation of protein-based therapeutics can occur during development, production, or storage and can lead to loss of efficacy and potential toxicity. Native mass spectrometry of a covalently linked pentameric monoclonal antibody complex with a mass of ∼800 kDa reveals several distinct conformations, smaller complexes, and abundant higher-order aggregates of the pentameric species. Charge detection mass spectrometry (CDMS) reveals individual oligomers up to the pentamer mAb trimer (15 individual mAb molecules; ∼2.4 MDa) whereas intermediate aggregates composed of 6-9 mAb molecules and aggregates larger than the pentameric dimer (1.6 MDa) were not detected/resolved by standard mass spectrometry, size exclusion chromatography (SEC), capillary electrophoresis (CE-SDS), or by mass photometry. Conventional quadrupole time-of-flight mass spectrometry (QTOF MS), mass photometry, SEC, and CE-SDS did not resolve partially or more fully unfolded conformations of each oligomer that were readily identified using CDMS by their significantly higher extents of charging. Trends in the charge-state distributions of individual oligomers provides detailed insight into how the structures of compact and elongated mAb aggregates change as a function of aggregate size. These results demonstrate the advantages of CDMS for obtaining accurate masses and information about the conformations of large antibody aggregates despite extensive overlapping m/z values. These results open up the ability to investigate structural changes that occur in small, soluble oligomers during the earliest stages of aggregation for antibodies or other proteins

Eddy formation near the west coast of Greenland

Author Posting. © American Meteorological Society, 2008. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 38 (2008): 1992-2002, doi:10.1175/2008JPO3669.1.This paper extends A. Bracco and J. Pedlosky’s investigation of the eddy-formation mechanism in the eastern Labrador Sea by including a more realistic depiction of the boundary current. The quasigeostrophic model consists of a meridional, coastally trapped current with three vertical layers. The current configuration and topographic domain are chosen to match, as closely as possible, the observations of the boundary current and the varying topographic slope along the West Greenland coast. The role played by the bottom-intensified component of the boundary current on the formation of the Labrador Sea Irminger Rings is explored. Consistent with the earlier study, a short, localized bottom-trapped wave is responsible for most of the perturbation energy growth. However, for the instability to occur in the three-layer model, the deepest component of the boundary current must be sufficiently strong, highlighting the importance of the near-bottom flow. The model is able to reproduce important features of the observed vortices in the eastern Labrador Sea, including the polarity, radius, rate of formation, and vertical structure. At the time of formation, the eddies have a surface signature as well as a strong circulation at depth, possibly allowing for the transport of both surface and near-bottom water from the boundary current into the interior basin. This work also supports the idea that changes in the current structure could be responsible for the observed interannual variability in the number of Irminger Rings formed.AB is supported by WHOI unrestricted funds, JP by the National Science Foundation OCE 85108600, and RP by 0450658

Simulation of natural convective boundary layer flow of a nanofluid past a convectively heated inclined plate in the presence of magnetic field

AbstractThis paper deals with the numerical simulation of transient magnetohydrodynamics natural convective boundary layer flow of a nanofluid over an inclined plate. In the modeling of nanofluids, dynamic effects including the Brownian motion and thermophoresis are taken into account. Numerical solutions have been computed via the Galerkin-finite element method. The effects of angle of inclination, buoyancy-ratio parameter, Brownian motion, thermophoresis and magnetic field are taken into account and controlled by non-dimensional parameters. To compute the rate of convergence and error of the computed numerical solution, the double mesh principle is used. Similarity solutions are calculated and presented graphically for non-dimensional velocity, temperature, local rate of heat and mass transfer with pertinent parameters. The modified Nusselt number decreases with increasing inclination angle, buoyancy-ratio parameter, Brownian motion and thermophoresis parameter, whereas it increases with increasing Prandtl number. Validation of the results is achieved with earlier results for forced convective flow and non-magnetic studies. Such problems have several applications in engineering and petroleum industries such as electroplating, chemical processing of heavy metals and solar water heaters. External magnetic fields play an important role in electrical power generation, inclination/acceleration sensors, fine-tuning of the final materials to industrial specification because of their controlling behaviour on the flow characteristics of nanofluids

Buoy observations from the windiest location in the world ocean, Cape Farewell, Greenland

Author Posting. © American Geophysical Union, 2008. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 35 (2008): L18802, doi:10.1029/2008GL034845.Cape Farewell, Greenland's southernmost point, is a region of significant interest in the meteorological and oceanographic communities in that atmospheric flow distortion associated with the high topography of the region leads to a number of high wind speed jets. The resulting large air-sea fluxes of momentum and buoyancy have a dramatic impact on the region's weather and ocean circulation. Here the first in-situ observations of the surface meteorology in the region, collected from an instrumented buoy, are presented. The buoy wind speeds are compared to 10 m wind speeds from the QuikSCAT satellite and the North American Regional Reanalysis (NARR). We show that the QuikSCAT retrievals have a high wind speed bias that is absent from the NARR winds. The spatial characteristics of the high wind speed events are also presented.The support of the Canadian Foundation for Climate and Atmospheric Science, the support of the National Science Foundation grant OCE-0450658as well as the Natural Environmental Research Council grant NE/C003365/1

Natural convection in a square cavity with uniformly heated and/or insulated walls using marker-and-cell method

In this study, a numerical investigation has been performed using the computational Harlow-Welch MAC (Marker and Cell) finite difference method to analyse the unsteady state two-dimensional natural convection in lid-driven square cavity with left wall maintained at constant heat flux and remaining walls kept thermally insulated. The significant parameters in the present study are Reynolds number (Re), thermal Grashof number (Gr) and Prandtl number (Pr) and Peclét number (Pe =PrRe). The structure of thermal convection patterns is analysed via streamline, vorticity, pressure and temperature contour plots. The influence of the thermophysical parameters on these distributions is described in detail. Validation of solutions with earlier studies is included. Mesh independence is also conducted. It is observed that an increase in Prandtl number intensifies the primary circulation whereas it reduces the heat transfer rate. Increasing thermal Grashof number also decreases heat transfer rates. Furthermore the isotherms are significantly compressed towards the left (constant flux) wall with a variation in Grashof number while Peclét number is fixed. The study is relevant to solar collector heat transfer simulations and also crystal growth technologies