Selective isolation and antimicrobial activity of rare actinomycetes from mangrove sediment of Karwar

The mangrove ecosystem is a largely unexplored source for actinomycetes. Various pretreatment procedures and selective media were applied to assess the optimal conditions for the isolation of rare actinomycetes from Mangrove sediment. Pretreatment of wet-heating for 15 min at 70oC and phenol treatment of soil suspension were the most effective methods for the isolation of those microorganisms. Hair hydrolysate vitamin agar (HHVA) was the most suitable medium for the recovery of rare actinomycetes. Fifty-three rare actinomycete strains were chosen using selective isolation approaches, then morphological and chemical properties of the isolates were determined. The isolates belonged to one of the following genus, Micromonospora, Microbispora, Actinoplanes, and Actinomadura. Later Micromonospora and Actinomadura were selected for antimicrobial activity. Minimum bactericidal concentration (MBC) of ethyl acetate extract against Staphylococcus aureus were 1.20 mg/ml for Micromonospora species and 5mg/ml for Actinomadura species. Thin layer chromatography (TLC) of the ethyl acetate extracts were carried out in duplicate using Chloroform: methanol (4:1) as solvent system and Tetracycline as reference antibiotic. Under UV light they gave greenish yellow spots with Rf value 0.85 for the antimicrobial from Actinomadura species and 0.88 for that from Micromonospora species. In bioautography (using Staphylococcus auras as test organism) inhibition zones were obtained and they were associated with the yellowish green spots of the chromatogram as detected under UV light. This may indicate the same compounds were responsible for the antibacterial activity of those actinomycetes isolates. ÂÂ

Public Integrity Auditing for Dynamic Data Sharing With Multiuser Modification

In cloud storage systems, information proprietors have their information on cloud servers furthermore, clients (information customers) can get to the information from cloud servers. Because of the information outsourcing, be that as it may, this new worldview of information facilitating administration additionally presents new security challenges, which requires an autonomous evaluating administration to check the information honesty in the cloud. In huge scale distributed storage frameworks, the information might be refreshed powerfully, so existing remote uprightness checking strategies served for static chronicle information are no longer appropriate to check the information uprightness. Accordingly, a proficient and secure dynamic inspecting convention is wanted to persuade information proprietors that the information is accurately put away in the cloud. In this section, we initially present an evaluating structure for cloud capacity frameworks. At that point, we depict Third-party Auditing Scheme a proficient and security saving evaluating convention for distributed storage, which can likewise bolster information dynamic operations and cluster reviewing for both various proprietors what's more

Chiral spin textures creation and dynamics in a rectangular nanostructure

Controlled creation of stable chiral spin textures is required to use them as an energy-efficient information carrier in spintronics. Here we have studied the stable creation of isolated chiral spin texture (skyrmion and antiskyrmion) and its pair through the magnetization reversal of a rectangular nanostructure using spin-polarized currents. An isolated spin texture is created through a negative current pulse. Dynamics of the stable spin texture are explored under external magnetic fields, and the resonant frequencies are calculated. A stable skyrmion pair is created using an asymmetric current pulse, and their interaction is studied using the Thiele equation. The stability of isolated or paired spin texture depends on the DMI strength, spin-polarized current density, and pulse duration. In addition, the stability of the skyrmion pair depends on their initial separation, and a threshold for the separation between skyrmions of 78 nm is observed.Comment: 29 pages, 11 figures, 2 extra figure

Comparative Study of Sink Node Placement Strategies of Wireless Sensor Network

One of the fundamental design challenges in designing a Wireless Sensor Network (WSN) is to be more maximize the network lifetime, as each sensor node of the network is equipped with a limited power battery. Wireless Sensor Networks are rapidly growing area of research and commercial development. Meanwhile it draws attention of many researchers because of the enormous scope of its applications in numerous areas. A Wireless Sensor Network (WSN) consists of large number of spatially distributed autonomous sensors to monitor physical environment conditions, such as temperature, sound, humidity, pressure, light etc. and pass their data often called raw data through the network to Base Station which is often called Sink. The sink forms the gateway between the WSN and end-user application. In real time applications sensors collect data and transfer to the sink. Generally Sensors have limited range and less battery life. In this paper our main goal is to increase the network life time of sensors and reduce their energy consumption of the network. In this paper two sink placement strategies are implemented along with an existing strategy geographical sink placement strategy (GSP) by placing sink in an appropriate area to cover maximum number no of sensors in the region of an network. The advantages of these two strategies were analyzed and compare with an existing strategy

Mixed Layer Budget Terms on Acoustic Propagation A Study based on the Butterfly Track Experiment in the South Eastern Arabian Sea

A butterfly type of repeat track cruise was carried out in the South Eastern Arabian Sea (off Minicoy) onboard INS Sagardhwani during July 2016 to Aug 2016. We have also made use of the data from OMNI buoy, AD09, which is about 6 km close to the centre station of butterfly track. Air sea flux, the horizontal current data from AD09 and the time series data collected from the butterfly experiment were analyzed to compute the mixed layer heat and salt budget. The short-term thermo-haline variability off Minicoy, relative contribution of heat/salt budget terms in MLD and its effects on acoustic propagation are addressed in this paper. In this study, we found that most dominating term in the mixed layer heat budget estimation is net surface heat flux followed by the advective terms. However the salinity in the mixed layer is dominated by the contribution of buoyancy mixing due to night time evaporative cooling. During the calm, sunny day, the so-called afternoon effect due to the diurnal heating restricts the sonar range. But during the windy day, the wind/wave mixing prevents the warming of the surface layer which in turn enhances the sonar range. Similarly, the night time cooling also enhance the acoustic propagation range. The presence of Arabian Sea High Salinity Watermass in the surface layer also enhances the acoustic propagation

Free radical 5-exo-dig cyclization as the key step in the synthesis of bis-butyrolactone natural products: experimental and theoretical studies

Radical cyclization reactions were performed by 5-exo-dig mode to yield cis-fused bicyclic systems, leading to the synthesis of bis-butyrolactone class of natural products. The study was aimed at understanding the impact of alkyl side chains of furanoside ring systems in L-ara configuration on the radical cyclization. It was amply demonstrated by experimental studies that the increase in the length of the alkyl side chain has an effect on the cyclization: while efficient cyclization reactions could be realized with methyl and ethyl side chains, the yields were significantly reduced in the case of n-pentyl side chain. Theoretical studies using DFT and (RO)MP2 methods were carried out to analyze the influence of the substitution pattern on the cyclization barriers

Assessment of genetic diversity in blackgram [Vigna mungo (L.) Hepper] germplasm

Genetic diversity analysis was carried out with 50 black gram genotypes by using Mahalanobis D2 statistics for ten quantitative traits. All the genotypes were grouped into ten non-overlapping clusters. Cluster I was the largest with 19 genotypes, followed by cluster II with 14 genotypes, cluster III with 8 genotypes, clusters VII and IX were digenotypic, while the remaining clusters IV, V, VI, VII and X were monogenotypic. The maximum inter-cluster distance was observed between cluster VII and IX. Cluster IX had recorded high mean values for most of the traits. Selection of genotypes from these diverse clusters with high mean performance and more per cent contribution as parents in the crossing may result in superior combinations along with maximum variability in the segregating generations. Hence, crosses among the genotypes viz., LBG 623 (cluster I), LBG 787 (cluster III), DKU 87 (cluster II) and VBN 8 (cluster VII) may result in production of superior pureline varieties in black gram. Among the traits, pods/plant contributed maximum towards the total divergence

Telemedicine across the globe-position paper from the COVID-19 pandemic health system resilience PROGRAM (REPROGRAM) international consortium (Part 1)

Coronavirus disease 2019 (COVID-19) has accelerated the adoption of telemedicine globally. The current consortium critically examines the telemedicine frameworks, identifies gaps in its implementation and investigates the changes in telemedicine framework/s during COVID-19 across the globe. Streamlining of global public health preparedness framework that is interoperable and allow for collaboration and sharing of resources, in which telemedicine is an integral part of the public health response during outbreaks such as COVID-19, should be pursued. With adequate reinforcement, telemedicine has the potential to act as the “safety-net” of our public health response to an outbreak. Our focus on telemedicine must shift to the developing and under-developing nations, which carry a disproportionate burden of vulnerable communities who are at risk due to COVID-19

Loss-of-function mutations in UDP-Glucose 6-Dehydrogenase cause recessive developmental epileptic encephalopathy

AbstractDevelopmental epileptic encephalopathies are devastating disorders characterized by intractable epileptic seizures and developmental delay. Here, we report an allelic series of germline recessive mutations in UGDH in 36 cases from 25 families presenting with epileptic encephalopathy with developmental delay and hypotonia. UGDH encodes an oxidoreductase that converts UDP-glucose to UDP-glucuronic acid, a key component of specific proteoglycans and glycolipids. Consistent with being loss-of-function alleles, we show using patients’ primary fibroblasts and biochemical assays, that these mutations either impair UGDH stability, oligomerization, or enzymatic activity. In vitro, patient-derived cerebral organoids are smaller with a reduced number of proliferating neuronal progenitors while mutant ugdh zebrafish do not phenocopy the human disease. Our study defines UGDH as a key player for the production of extracellular matrix components that are essential for human brain development. Based on the incidence of variants observed, UGDH mutations are likely to be a frequent cause of recessive epileptic encephalopathy.</jats:p