A dual sensor device to estimate fluid flow velocity at diffuse hydrothermal vents

Author Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Deep Sea Research Part I: Oceanographic Research Papers 56 (2009): 2065-2074, doi:10.1016/j.dsr.2009.06.008.Numerous attempts have been made over the last thirty years to estimate fluid flow rates at hydrothermal vents, either at the exit of black smoker chimneys or within diffuse flow areas. In this study, we combine two methods to accurately estimate fluid flow velocities at diffuse flow areas. While the first method uses a hot film anemometer that performs high frequency measurements, the second allows a relatively rapid assessment of fluid flow velocity through video imagery and provides in situ data to calibrate the sensor. Measurements of flow velocities on hydrothermal diffuse flow areas were obtained on the Mid-Atlantic Ridge (MAR). They range from 1.1 to 4.9 mm/sec., at the substratum level, in low temperature (4.5 to 16.4°C) diffuse flow areas from the Tour Eiffel sulfide edifice. A strong correlation was observed between fluid flow velocities and temperature, supporting the possible use of temperature as a proxy to estimate flow rates in diffuse flow areas where such a simple linear flow/temperature relation is shown to dominate.The first part of this research was sponsored by a NOAA/NURP grant award #NA96RU0221 and NSF grant OCE-9901563 to MKT and JS. JS was also supported by a FCAR (Quebec) post-doctoral fellowship. The last part of the project was supported through the ANR DEEP OASES (ANR06 BDV005)

Dissolved and particulate metals (Fe, Zn, Cu, Cd, Pb) in two habitats from an active hydrothermal field on the EPR at 13°N

Le texte intégral est accessible via Archimer: http://archimer.ifremer.fr/doc/2008/publication-3830.pdfInternational audienceThe distribution of Fe, Cu, Zn, Pb, Cd between the dissolved ( 2 μm) fractions was measured after in-situ filtration in two hydrothermal habitats. The total metal concentration ranges exhibit a clear enrichment compared with the seawater concentration, accounting for the hydrothermal input for all the metals considered. Iron is the predominant metal (5-50 μM) followed by Zn and Cu. Cd and Pb are present at the nM level. At the scale studied, the behavior of temperature, pH and dissolved iron is semi-conservative whereas the other dissolved and particulate metals are characterized by non-conservative patterns. The metal enrichment of the > 2 μm fraction results from the settlement and accumulation of particulate matter close to the organisms, acting as a secondary metal source. The enrichment observed in the dissolved fraction can be related to the dissolution or oxidation of particles (mainly polymetallic sulfide) or to the presence of small particles and large colloids not retained on the 2 μm frit. SEM observations indicate that the bulk particulate observed is characteristic of crystalline particles settling rapidly from the high temperature smoker (sphalerite, wurtzite and pyrite), amorphous structures and eroded particles formed in the external zone of the chimney. Precipitation of Zn, Cu, Cd and Pb with Fe as wurtzite, sphalerite and pyrite is the main process taking place within the area studied and is semi-quantitative. The distribution of the dominant observed fauna has been related to the gradient resulting from the dilution process, with the alvinellids worms colonizing the hotter and more variable part of the mixing zone, but also to the metallic load of the mixing zone. Dissolved and particulate metal concentrations are therefore necessary abiotic factors to be studied in a multiparametric approach to understand the faunal distribution in hydrothermal ecosystems

Relationship between the occurrence of filamentous bacteria on Bathymodiolus azoricus shell and the physiological and toxicological status of the vent mussel

En libre-accès sur Archimer : http://archimer.ifremer.fr/doc/2009/publication-6821.pdfInternational audienceThe edifice walls of the Eiffel Tower hydrothermal vent site (Mid-Atlantic Ridge, Lucky Strike vent field) are populated with dense communities of dual symbioses harboring vent mussel Bathymodiolus azoricus, some of which are covered by white filamentous mats belonging to sulfur-oxidizing bacteria. Mussels were collected in both the presence and absence of the filamentous bacteria. A sample of the filamentous bacteria was collected and water measurements of temperature, CH4 and H2S were recorded at the collection area. The whole soft tissues were analyzed for total lipid, carbohydrate and total protein. Metallothioneins and metals (Cu, Fe and Zn) levels were determined in the major organs. The results showed no significant physiological and toxicological evidence that emphasizes the influence of associated sulfur-oxidizing filamentous bacteria on B. azoricus mussel shells. However, B. azoricus mussel seems to be well adapted to the assorted physico-chemical characteristics from the surrounding environment since it is able to manage the constant fluctuation of physico-chemical compounds

The EGIM, EMSO generic instrument module, step towards standardization

Peer ReviewedPostprint (published version

Dual Relief of T-lymphocyte Proliferation and Effector Function Underlies Response to PD-1 Blockade in Epithelial Malignancies

Although understanding of T-cell exhaustion is widely based on mouse models, its analysis in patients with cancer could provide clues indicating tumor sensitivity to immune checkpoint blockade (ICB). Data suggest a role for costimulatory pathways, particularly CD28, in exhausted T-cell responsiveness to PD-1/PD-L1 blockade. Here, we used single-cell transcriptomic, phenotypic, and functional approaches to dissect the relation between CD8+ T-cell exhaustion, CD28 costimulation, and tumor specificity in head and neck, cervical, and ovarian cancers. We found that memory tumor–specific CD8+ T cells, but not bystander cells, sequentially express immune checkpoints once they infiltrate tumors, leading, in situ, to a functionally exhausted population. Exhausted T cells were nonetheless endowed with effector and tumor residency potential but exhibited loss of the costimulatory receptor CD28 in comparison with their circulating memory counterparts. Accordingly, PD-1 inhibition improved proliferation of circulating tumor–specific CD8+ T cells and reversed functional exhaustion of specific T cells at tumor sites. In agreement with their tumor specificity, high infiltration of tumors by exhausted cells was predictive of response to therapy and survival in ICB-treated patients with head and neck cancer. Our results showed that PD-1 blockade–mediated proliferation/reinvigoration of circulating memory T cells and local reversion of exhaustion occur concurrently to control tumors

EMSO ERIC: A challenging infrastructure to monitor Essential Ocean Variables (EOVs) across European Seas

Special issue 9th MARTECH: International Workshop on Marine Technology: 16-18 June 2021, Vigo, Spain.-- 2 pages, 1 figureThe European Multidisciplinary Seafoor and water Column Observatory (EMSO, www.emso.eu) is a distributed research infrastructure (RI), composed of fxed-point deep-sea observatories and shallow water test sites at strategic environmental locations from the southern entrance of the Arctic Ocean all the way through the North Atlantic through the Mediterranean to the Black Sea. Working as a single powerful system, it is a valuable new tool for researchers and engineers looking for long time series of high-quality and high-resolution data to study and continuously monitor complex processes interactions among the geosphere, biosphere, hydrosphere and atmosphere, as well as to test, validate and demonstrate new marine technologiesPeer reviewe

Intermediate Scientific report “Working with two deep-sea ROVs” (French Oceanographic Fleet)

As part of the evolution plan of the French Oceanographic Fleet, the Directory Steering Committee and the MESRI have confirmed in March 2018 to withdraw the usage of the submarine HOV Nautile in the medium term and to begin investigating of a “scenario with two deep-sea ROVs”, including the revamping and modernizing of the ROV Victor6000. The outcomes are subject to the positive accreditation by the Direction Steering Committee of the initial (Phase 0) conclusions to be carried out by the Fleet Directorate, so as to assess its technical and budgetary feasibility. The technical Phase 0 of this project had to take into consideration the current technological advances (marine instrumentation or in other domains) or early-stage developments, as well as the emergence of new needs. The approach should provide a project calendar enabling still the availability of two deep-sea operational vehicles to the scientific community and providing the time necessary to acquire the financial support to build the new vehicle. The outcomes of the technical Phase 0 have been presented in December 2018 to the FOF – CS, then to the Direction Steering Committee. Following the positive evaluation by the FOF – CS, the Direction Steering Committee decided to launch the Phase 1 aiming at a preliminary definition with the following objectives: ● Definition of the scientific needs and requirements associated with the potential scenario of “working with 2 deep-sea ROVs”; ● Definition of the technical functionalities derived from the scientific requirements; ● Writing of a technical report of the system according to the definition of the scientific need; ● This will be done in close link to the revamping and modernizing stages of the ROV Victor6000. The final report will provide the grounds for the technological development of a new deep-sea ROV combined with the modernization of the ROV Victor6000, while gathering, scrutinizing and complying with the scientific needs.