Détermination Des Teneurs En Mercure (HG) Total Chez La Moule Perna Perna Et L’oursin Echinometra Lucunter De La Baie De Soumbédioune (Sénégal) Et Évaluation Des Risques Sanitaires

La baie de Soumbédioune où s’effectue une importante activité de cueillette et de commercialisation de fruits de mer, constitue le réceptacle d’énormes quantités d’eaux usées et de déchets provenant du canal 4 et des activités artisanales qui s’y effectuent. C’est dans ce contexte qu’une étude visant à déterminer le niveau de contamination par le mercure des moules (Perna perna) et des oursins (Echinometra lucunter) et à évaluer les risques sanitaires liés à consommation de ces produits a été réalisée. Elle a consisté à collecter des échantillons (crus et transformés) de ces deux espèces durant les périodes d’abondance et de non-abondance en 2018. L’analyseur direct de mercure DMA-80 a été utilisé pour déterminer teneurs en mercure contenues dans ces espèces. Les résultats obtenus après analyses montrent la présence de ce métal très toxique dans les parties comestibles de ces espèces mais à des concentrations en deçà de la norme établie par l’agence américaine pour la protection de l’environnement. Ces concentrations varient entre 0,0172 et 0,0475 mg.kg-1 pour les moules contre 0,0153 et 0,0274 mg.kg-1 pour les oursins. Les indices de risque sanitaire obtenus (oscillant entre 0,070 et 0,844) sont inférieurs à 1 révèlent que la consommation de ces espèces reste pour le moment sans danger. Néanmoins, elles devraient être consommées modérément en raison de la capacité du mercure à se bioaccumuler dans les organismes. Soumbedioune Bay, marked by an intense activity of exploitation and marketing of seafood, is the receptacle of huge quantities of wastewater from channel 4 and artisanal activities which are carried out there. That’s why a study aiming to determine the level of mercury contamination of mussels (Perna perna) and sea urchins (Echinometra lucunter) and to assess the health risks associated with their consumption was performed. It consisted to collect samples (non-transformed and transformed products) of these two species during the periods of abundance and non-abundance in 2018. The direct mercury analyzer DMA-80 was used to determine the mercury contents contained in these species. The results obtained after analyzes show the presence of this very toxic metal in the edible parts of these species but at concentrations below the standard established by the American agency for the protection of the environment. These concentrations vary between 0.0172 and 0.0475 mg.kg-1 for mussels against 0.0153 and 0.0274 mg.kg-1 for sea urchins. The health risk indices obtained (oscillating between 0.070 and 0.844) less than 1 reveal that the consumption of these species remains safe for the moment. However, they should be consumed moderately due to the ability of mercury to bioaccumulate in organisms

In Vitro Assessment Of The Antiplasmodial Activity Of Three Plants Extracts Used In Local Traditional Medicine In Saloum (Senegal)

Against the scourge of malaria in Africa and the development of chemo-resistance, discovering new natural cures is a major concern for many researchers. As a consequence, the present study was carried out in order, first, to identify the phytochemical compounds, and second, to assess the antiplasmodial activity, of three medicinal plants extracts, selected from an ethno-botany survey conducted in Senegal. The parts of the plants used were Strychnos spinosa (Strychnaceae) leaves and stems, Combretum glutinosum (Combretaceae) barks, and the whole aerial part of Pennisetum polystachion (Poaceae). Among these plants, the hydromethanolic extracts of Strychnos spinosa stems showed the most important activity against Plasmodium falciparum strains, with a IC50 of 15 μg/ml, followed by the leaves chlorophormic extracts and the ethyl acetate extracts of Pennisetum polystachion, with respective IC50 of 20 μg/ml and 21 μg/ml. Combretum glutinosum extracts showed the least important activity, in all tests

Biodiversité Et Structure Des Ligneux De La Forêt Classée De Maka Diama Dans Le Delta Du Fleuve Sénégal

Le barrage anti-sel de Diama, érigé dans la zone centrale du delta du fleuve Sénégal, a créé de profondes mutations au niveau de l’environnement de la vallée. La Forêt Classée de Maka Diama (FCMD), aménagée au niveau de ce delta en 1934 par l’administration coloniale n’est pas épargnée. La présente étude fait l’état de lieux de la forêt, elle décrit la composition floristique et la structure des ligneux. La base de sondage a été des images satellitaires indiquant les différentes entités écologiques de cette forêt. La végétation ligneuse a été caractérisée par inventaire suivant une approche dendrométrique. Les résultats de cette étude montrent que la FCMD présente une biodiversité relativement faible avec 13 espèces appartenant à 11 genres et 9 familles. L’espèce Acacia tortilis var.raddiana (Savi) Brenan (39,67 %) est la plus abondante, elle est suivie de Salvadora persica L. (16,99%) et de Tamarix senegalensis DC (12,13 %), Euphorbia balsamifera Ait. (11,21 %) et Prosopis juliflora (SW.) (10,38 %). La présence de T. senegalensis considéré comme un indicateur de salinité, pourrait aussi expliquer cette faible biodiversité des ligneux. La FCMD est globalement arbustif. C’est un bassin versant constitué d’une relique de savane arborée au centre du bassin qui constitue son noyau (F1) et qui évolue en une steppe arbustive vers la périphérie (F3). Avec la diversité des activités menées autour de la forêt, il semble indispensable de prendre des mesures appropriées de limitation ou d’interdiction d’accès, dans l’intérêt de la protection de la biodiversité. The Diama anti-salt dam, built in the central zone of the Senegal River delta, has created profound changes in the environment of the entire valley. The Maka Diama Classified Forest (FCMD), developed at the level of this delta in 1934 by the colonial administration is not spared. This study presents an inventory of the forest, describing the floristic composition and structure of ligneous trees. The sampling frame was satellite images showing the different ecological features of this forest. Woody vegetation was characterized by inventory using a dendrometric approach. The results of this study show that the FCMD has a relatively low biodiversity with only 13 species belonging to 11 genera and 9 families. Acacia tortilis var.raddiana (Savi) Brenan (39.67%) is more abundant, followed by Salvadora persica L. (16.99%), Tamarix senegalensis DC (12.13%), Euphorbia balsamifera Ait. (11.21%) and Prosopis juliflora (SW.) (10.38%). The presence of T. senegalensis, considered as an indicator of salinity, could also explain the low plant biodiversity observed on ligneous trees. The FCMD is generally shrubby. It is a catchment area made up of a relic of savannah trees in the centre of the basin which constitutes its nucleus (F1) and which evolves towards a shrub steppe towards the periphery (F3). With the diversity of activities carried out around the forest, it seems essential to take appropriate measures to limit or prohibit access, in the interestn of protecting biodiversity

Biochemical composition and nutritional value of Balanites aegyptiaca (L.) Del fruit pulps from Northern Ferlo in Senegal

Balanites aegyptiaca, a forest species of socio-economic interest for rural people, was chosen to be planted in the frame-work of the Great Green Wall project in Senegal. Although in vastly demand during food scarcity period before crops harvest, the species’ fruit is poorly valued despite its important production. In this study, the fruit pulps were harvested at maturity and the biochemical characteristics were accessed in order to better understand the fruit’s nutritional value. The moisture, sugars, proteins, amino acids, ash, fat, minerals and some vitamins were determined by standard methods of analysis. The results indicated that the fruits are a good alimentary source of sugar, minerals (mainly potassium) and ascorbic acid. The pulp proteins were qualitatively balanced, but were present only in weak quantities. Its caloric value is high due to the high concentration of sugars. Low humidity should allow a fairly good postharvest fruit conservation. The dietary intake of this fruit for local people is very valuable especially in terms of nutrition.Keywords: Balanites aegyptiaca, pulp, biochemical, nutritional valueAfrican Journal of Biotechnology, Vol. 13(2), pp. 336-342, 8 January, 201

Effet de la variété et du type de terreau sur la croissance et le développement des plants de Mangifera indica L, Casamance

Pour contribuer à l’amélioration de la production de mangue en Casamance cette étude s’est fixé comme objectif d’évaluer l’effet de la variété et du type de terreau sur la croissance et le développement des plants de Mangifera indica L. Un dispositif en split plot à deux facteurs dont le facteur substrat décliné en 3 modalités (terreau de Cailcédrat, terreau manguier et terreau d’anacardier) et puis le facteur variété constitué de cinq modalités (Pince, Kouloubadasèky, Siéra Léone, Diourou et Papaye). La variété Siéra Léone a donné les meilleurs résultats en termes de production foliaire (19,99 feuilles/plant), hauteur (29,76±6,58 cm) et diamètre au collet des plants (6,97 mm). Concernant l’effet du type de terreaux, le diamètre au collet des plants le plus gros (6,49±1,17 mm) a été enregistré avec le terreau de manguier, et la production foliaire des plants la plus élevée (10,54 feuilles/plant) a été notée avec les terreaux de manguier et de caïlcédrat. La hauteur des plants n’a pas varié significativement (P=0,6) en fonction du type de terreau. La biomasse racinaire moyenne a varié significativement (P= 0,0001) selon le traitement. Elle est plus importante avec le traitement variété Siéra Léone sur terreau de caïlcédrat avec 6,85±2,28g/plant. Aucune différence significative n’a été observée pour la biomasse foliaire (P= 0,086) et caulinaire (P=0,33) entre les traitements. Ces résultats permettront de constituer une base de données utile pour la reconstitution des vergers de manguiers en Casamance

Characterization of element and mineral content in Artemisia annua and Camellia sinensis leaves by handheld X-ray fluorescence

Tea infusion is the most frequently worldwide consumed beverage next to water, with about 20 billion cups consumed daily. Artemisia annua leaves contain comparable levels of nutrients and mineral elements (dry matter basis) to many marketed tea (Camellia sinensis) leading us to suspect that this crop could also serve as an alternative source of nutrients for humans. Analyzer moveable X-ray fluorescence is used to evaluate the content of major, minor and toxic elements in A. annua from two different countries compared to six marketed tea in Senegal. To ensure qualified results, certified reference materials were used to perform the calibration. The very low and often negligible levels of inherent elements in the leaves, which are far below recommended toxic levels, establishes A. annua and selected marketed tea as a good reservoir of elements that might favour its use as a potential herbal tonic by humans. The mineral elements are present in different kinds of herbal leaves in various proportions depending on soil composition and the climate in which the plant grows.Keywords: X-ray fluorescence (XRF), Artemisia annua, Camellia sinensis, elements, leaves, medicinal plantAfrican Journal of Biotechnology Vol. 12(26), pp. 4179-418

Evaluation of the use of birth control followed in women's hospital regional Saint Louis (Senegal) in 2014

Background: To evaluate the use of contraceptive methods among women followed at Saint Louis Health Centre.Methods: This was a descriptive cross-sectional study of all recue clients in the Obstetrics and Gynecology department of the Regional Hospital of St. Louis. She ran from 1 January 2014 to 31 December 2014, 12 months.Results: The mean age was 28.8 years. The most common demographic characteristics were the Wolof ethnic group (64.5%), married the marital status (97.9%), they were not in school in 46.3%. The main sources of information on the use of contraception were health workers in 80.3%, friends and relatives (8.5%) followed by husbands (2.5%), the media represented 6 0% and 0.1% posters. The injection method was the most used method (42.8%) followed by implants (36.2%), pills (14.9%) and tubal ligation (4.4%). The IUD was the least used method with 1.7% of use. The main reasons given by women were spacing births (70.6%), to avoid unwanted pregnancy (3.90%), avoid early pregnancies (1.20%) and of other unspecified reasons in 24.3%. In this study, no accidental pregnancy was reported. Indeed, 79.4% of women had respected the monitoring schedule of monitoring visits. They had abandoned contraception in 23.5%.Conclusions: This study shows the achievements and shortcomings in relation to the nature and completeness of the information provided. Strengthening the awareness and training will reduce missed opportunities for family planning, and indirect; reduce the rate of unmet need for FP in our country

One-Year Clinical Evaluation of the Bonding Effectiveness of a One-Step, Self-Etch Adhesive in Noncarious Cervical Lesion Therapy

This study evaluated the one-year clinical performance of a one-step, self-etch adhesive (Optibond All-in-One, Kerr, CA, USA) combined with a composite (Herculite XRV Ultra, Kerr Hawe, CA, USA) to restore NCCLs with or without prior acid etching. Restorations performed by the same practitioner were evaluated at baseline and after 3, 6, and 12 months using modified USPHS criteria. At 6 months, the recall rate was 100%. The retention rate was 84.2% for restorations with prior acid etching, but statistically significant differences were observed between baseline and 6 months. Without acid etching, the retention rate was 77%, and no statistically significant difference was noted between 3 and 6 months. Marginal integrity (93.7% with and 87.7% without acid etching) and discoloration (95.3% with and 92.9% without acid etching) were scored as Alpha or Bravo, with better results after acid etching. After one year, the recall rate was 58.06%. Loss of pulp vitality, postoperative sensitivity, or secondary caries were not observed. After one year retention rate was of 90.6% and 76.9% with and without acid conditioning. Optibond All-in-One performs at a satisfactory clinical performance level for restoration of NCCLs after 12 months especially after acid etching

Evolution of the Pyrethroids Target-Site Resistance Mechanisms in Senegal: Early Stage of the Vgsc-1014F and Vgsc-1014S Allelic Frequencies Shift.

The evolution and spread of insecticide resistance mechanisms amongst malaria vectors across the sub-Saharan Africa threaten the effectiveness and sustainability of current insecticide-based vector control interventions. However, a successful insecticide resistance management plan relies strongly on evidence of historical and contemporary mechanisms circulating. This study aims to retrospectively determine the evolution and spread of pyrethroid resistance mechanisms among natural Anopheles gambiae s.l. populations in Senegal. Samples were randomly drawn from an existing mosquito sample, collected in 2013, 2017, and 2018 from 10 sentinel sites monitored by the Senegalese National Malaria Control Programme (NMCP). Molecular species of An. gambiae s.l. and the resistance mutations at the Voltage-gated Sodium Channel 1014 (Vgsc-1014) locus were characterised using PCR-based assays. The genetic diversity of the Vgsc gene was further analyzed by sequencing. The overall species composition revealed the predominance of Anopheles arabiensis (73.08%) followed by An. gambiae s.s. (14.48%), Anopheles coluzzii (10.94%) and Anopheles gambiae-coluzii hybrids (1.48%). Both Vgsc-1014F and Vgsc-1014S mutations were found in all studied populations with a spatial variation of allele frequencies from 3% to 90%; and 7% to 41%, respectively. The two mutations have been detected since 2013 across all the selected health districts, with Vgsc-L1014S frequency increasing over the years while Vgsc-1014F decreasing. At species level, the Vgsc-1014F and Vgsc-1014S alleles were more frequent amongst An. gambiae s.s. (70%) and An. arabiensis (20%). The Vgsc gene was found to be highly diversified with eight different haplotypes shared between Vgsc-1014F and Vgsc-1014S. The observed co-occurrence of Vgsc-1014F and Vgsc-1014S mutations suggest that pyrethroid resistance is becoming a widespread phenomenon amongst malaria vector populations, and the NMCP needs to address this issue to sustain the gain made in controlling malaria

In vivo transcriptional profiling of Plasmodium falciparum

BACKGROUND: Both host and pathogen factors contribute to disease outcome in Plasmodium falciparum infection. The feasibility of studying the P. falciparum in vivo transcriptome to understand parasite transcriptional response while it resides in the human host is presented. METHODS: A custom made oligonucleotide array with probes based on the P. falciparum 3D7 laboratory strain chromosome 2 sequence was used to detect in vivo P. falciparum transcripts. This study analyzed transcripts from total RNA derived from small blood samples of P. falciparum infected patients and compared the in vivo expression profile to the in vitro cultivated 3D7 strain transcriptome. RESULTS: The data demonstrated that in vivo transcription can be studied from a small blood sample, despite the abundance of human RNA. The in vivo transcriptome is similar to the 3D7 ring stage transcriptome, but there are significant differences in genes encoding a sexual stage antigen and surface proteins. CONCLUSIONS: Whole genome transcription analysis of P. falciparum can be carried out successfully and further studies in selected patient cohorts may provide insight into parasite in vivo biology and defense against host immunity