In God We Trust and Other Fictions

A collection of stories whose themes center on the 2008 financial crisis

Development and collapse of an Oscillatoria bloom in Loch Leven during July 1994

During 1994, weekly spot-sampling of open water sites on Loch Leven took place from 16th March onwards. Very little difference between spot-sampling sites was observed from 16th March to 5th July. Throughout April and May there was a slow increase in levels of chlorophyll-a, followed by a rapid increase through June, reaching a maximum of 230 micrograms per litre. On 8th July there was a very rapid rise in water temperature, with a corresponding increase in dissolved oxygen and pH. At 0915 hours on 9th July there was a drop in all three variables. These changes were coincident with a marked reduction in chlorophyll-a values falling to 70 micrograms per litre on 12th July. On 16th July evidence of a resurgence of photosynthesis was observed

Falloff of the Weyl scalars in binary black hole spacetimes

The peeling theorem of general relativity predicts that the Weyl curvature scalars Psi_n (n=0...4), when constructed from a suitable null tetrad in an asymptotically flat spacetime, fall off asymptotically as r^(n-5) along outgoing radial null geodesics. This leads to the interpretation of Psi_4 as outgoing gravitational radiation at large distances from the source. We have performed numerical simulations in full general relativity of a binary black hole inspiral and merger, and have computed the Weyl scalars in the standard tetrad used in numerical relativity. In contrast with previous results, we observe that all the Weyl scalars fall off according to the predictions of the theorem.Comment: 7 pages, 3 figures, published versio

The Impact of Material Handling on Manufacturing Process Plan Selection

The total manufacturing cost for a part is determined by its process plan, and the total cost includes both processing and material handling costs. This research examines the cost impact of assuming an arbitrary layout when evaluating alternative process plans for production in a new facility. A designed experiment was developed to explore the impact of this assumption. The experiment includes seven different factors that define a manufacturing scenario. The factors are: material cost, material removal rate, part volume, possible blank types, load/unload time, joining rate, and labor rate. The objective of this research is to determine which of these factors have a large impact on whether the assumption of an arbitrary layout is likely to lead to error in selecting the lowest cost process plan, and to use the results to identify specific manufacturing scenarios where the arbitrary layout assumption is not appropriate. Four factors were determined to be important: part volume, load unload time, material cost, and labor rate. The relative importance of these factors was then evaluated, and some manufacturing scenarios are identified where layout optimization and process plan optimization should be integrated

Maternal Circulating Levels of Activin A, Inhibin A, sFlt-1 and Endoglin at Parturition in Normal Pregnancy and Pre-Eclampsia

Background: Maternal circulating levels of anti-angiogenic factors such as soluble fms-like tyrosine kinase-1 (sFlt-1), endoglin (sEng) and placental proteins like activin A and inhibin A are increased before the onset of pre-eclampsia. There is evidence for oxidative stress in pre eclampsia. Recently it was shown that placental oxygen concentration is related to sFlt-1 and inhibin A. In addition it is reported that oxidative stress markers are increased in placental tissue delivered after labour. Therefore, the objective of this study is to investigate if these proteins are altered in maternal circulation of labouring preeclampsia and normal pregnancies. Methodology: To assess the effects of labour, samples were taken from 10 normal pregnant (NP) and 10 pre-eclamptic (PE) women pre-labour, full dilation, placental delivery and 24 h. To assess the effects of placental delivery, plasma samples were taken from 10NP and 10PE women undergoing elective Caesarean section, pre-delivery, placental delivery and 10 min, 60 min and 24 h post delivery. SFlt-1 and sEng and activin A and inhibin A were measured using commercial and in house ELISA’s respectively. Results: The levels of sFlt-1 and sEng were significantly higher in PE compared to NP women in both groups. In labour, sFlt- 1 levels increased significantly at full dilatation in PE women, before declining by 24 hr. However there was no significant rise in sEng levels in labour. Activin A and inhibin A levels declined rapidly with placental delivery in NP and PE pregnancies. There was a significant rise in activin A levels during labour in PE compared to pre labour, but inhibin levels did not increase. Conclusion: Labour in pre-eclamptic women increases the levels of sFlt-1 and activin A. This pilot data suggests that increase in the maternal levels of these factors in labour could predict and/or contribute to the maternal syndrome postpartum.Citation: Reddy, A. et al. (2009). 'Maternal circulating levels of activin A, inhibin A, sFlt-1 and endoglin at parturition in normal pregnancy and pre-eclampsia', PLoS ONE 4(2): e4453. [Available at http://www.plosone.org]. © 2009 Reddy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Measurement of refractive index by nanoparticle tracking analysis reveals heterogeneity in extracellular vesicles

Introduction: Optical techniques are routinely used to size and count extracellular vesicles (EV). For comparison of data from different methods and laboratories, suitable calibrators are essential. A suitable calibrator must have a refractive index (RI) as close to that of EV as possible but the RI of EV is currently unknown. To measure EV, RI requires accurate knowledge of size and light scattering. These are difficult to measure as most EVs cannot be resolved by light microscopy and their diameter is smaller than the wavelength of visible light. However, nanoparticle tracking analysis (NTA) provides both size and relative light scattering intensity (rLSI) values. We therefore sought to determine whether it was possible to use NTA to measure the RI of individual EVs. Methods: NTA was used to measure the rLSI and size of polystyrene and silica microspheres of known size and RI (1.470 and 1.633, respectively) and of EV isolated from a wide range of cells. We developed software, based on Mie scattering code, to calculate particle RI from the rLSI data. This modelled theoretical scattering intensities for polystyrene and silica microspheres of known size (100 and 200 nm) and RI. The model was verified using data from the polystyrene and silica microspheres. Size and rLSI data for each vesicle were processed by the software to generate RI values. Results: The following modal RI measurements were obtained: fresh urinary EV 1.374, lyophilised urinary EV 1.367, neuroblastoma EV 1.393, blood EV 1.398, EV from activated platelets 1.390, small placental EV 1.364–1.375 and 1.398–1.414 for large placental EV (>200 nm). Large placental EV had a significantly higher RI than small placental EV (p<0.0001). The spread of RI values was narrower for small EV than for the more heterogeneous large EV. Discussion: Using NTA and Mie scattering theory, we have demonstrated that it is possible to estimate the RI of sub-micron EV using NTA data. EV typically had a modal RI of 1.37–1.39, whereas values of >1.40 were observed for some large (>200 nm) microvesicles. Conclusion: This method for measuring EV RI will be useful for developing appropriate calibrators for EV measurement

Acute imidacloprid exposure alters mitochondrial function in bumblebee flight muscle and brain

Peer reviewe

Astronomy below the survey threshold in the SKA era

Astronomy at or below the 'survey threshold' has expanded significantly since the publication of the original 'Science with the Square Kilometer Array' in 1999 and its update in 2004. The techniques in this regime may be broadly (but far from exclusively) defined as 'confusion' or 'P(D)' analyses (analyses of one-point statistics), and 'stacking', accounting for the flux-density distribution of noise-limited images co-added at the positions of objects detected/isolated in a different waveband. Here we discuss the relevant issues, present some examples of recent analyses, and consider some of the consequences for the design and use of surveys with the SKA and its pathfinders