Combined effect of ammonia stress and cell line age on CHO cell derived VRC01 monoclonal antibody glycosylation

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Socio-Emotional Functioning and Face Recognition Ability in the Normal Population

Recent research indicates face recognition ability varies within the normal population. To date, two factors have been identified that influence this cognitive process: the age and gender of the perceiver. In this paper, we examine the influence of socio-emotional functioning on face recognition ability. We invited participants with high and low levels of empathy (as indicated by the Empathy Quotient) to take part in a face recognition test. Participants were asked to study a set of faces, and at test viewed the studied faces intermixed with novel faces. As predicted, high empaths achieved higher scores in the face recognition test compared to low empaths. This pattern of findings provides further evidence that face recognition ability varies within the normal population, and suggests socio-emotional functioning may be an additional factor that influences face recognition ability

Without Face-to-Face Limits: Using Online Modules to Expand Specialty Focused Residency EBM Instruction for the ACGME Milestone Project

Objective: The Accreditation Council for Graduate Medical Education (ACGME) Milestone Project has created the need for specialty focused, developmentally tiered and competency based evidence-based medicine (EBM) instruction. The University of North Carolina at Chapel Hill Health Sciences Library is extending current staff capacity for face-to-face sessions by creating online module templates that can be adapted to meet the specific needs of more residency programs. Method: Testing the effectiveness, quality and usability of the draft templates is part of the project development plan. The specialty focused EBM face-to-face instruction session of the Clinical Based Year (CBY) anesthesiology residents' Academic Medicine Rotation is the model for the online template. In order to be able to compare the validity of the online modules in comparison with face-to-face sessions, baseline data was gathered from a pre-test/post-test completed by the ten anesthesiology resident participants in the 2015 program. The pre-test/post-test included five knowledge questions and two self-perception questions. After the face-to-face session, the anesthesiology residents were asked to review the online module version of the instruction and answer a brief survey about ease of use and preferred learning mode. Results: Seven of ten residents increased their number of correct answers on the post-test. There were no perfect scores on the pre-test and five perfect scores on the post-test. Three of ten residents indicated a higher self-perceived comfort level for completing a PubMed EBM search. Two of ten residents had an increase level of agreement that their PubMed searching skills are sufficient. Seven of ten residents evaluated the online module and rated it as clearly organized and easy to understand and use. Four would prefer to learn and practice the EBM content in a group session with an instructor, two did not have a preference and one strongly preferred to learn online. Conclusion: Pre-test/post-test data confirmed face-to-face instruction had a positive impact on EBM knowledge and moderately improved self-perceived comfort with EBM searching. This baseline data will be used to compare with residents who only use the online format in the future. Positive feedback on ease of using the online module confirms that the template is functional. A more formal objective evaluation is planned. The variety of learning preferences within this small group indicates that face-to-face instruction is preferred by some, but that online modules will better meet the needs of others and appear to be an adequate way to expand our overall reach

Osteocalcin signaling in myofibers is necessary and sufficient for optimum adaptation to exercise

Circulating levels of undercarboxylated and bioactive osteocalcin double during aerobic exercise at the time levels of insulin decrease. In contrast, circulating levels of osteocalcin plummet early during adulthood in mice, monkeys, and humans of both genders. Exploring these observations revealed that osteocalcin signaling in myofibers is necessary for adaptation to exercise by favoring uptake and catabolism of glucose and fatty acids, the main nutrients of myofibers. Osteocalcin signaling in myofibers also accounts for most of the exercise-induced release of interleukin-6, a myokine that promotes adaptation to exercise in part by driving the generation of bioactive osteocalcin. We further show that exogenous osteocalcin is sufficient to enhance the exercise capacity of young mice and to restore to 15-month-old mice the exercise capacity of 3-month-old mice. This study uncovers a bone-to-muscle feedforward endocrine axis that favors adaptation to exercise and can reverse the age-induced decline in exercise capacity

Pan-Cancer Analysis of lncRNA Regulation Supports Their Targeting of Cancer Genes in Each Tumor Context

Long noncoding RNAs (lncRNAs) are commonly dys-regulated in tumors, but only a handful are known toplay pathophysiological roles in cancer. We inferredlncRNAs that dysregulate cancer pathways, onco-genes, and tumor suppressors (cancer genes) bymodeling their effects on the activity of transcriptionfactors, RNA-binding proteins, and microRNAs in5,185 TCGA tumors and 1,019 ENCODE assays.Our predictions included hundreds of candidateonco- and tumor-suppressor lncRNAs (cancerlncRNAs) whose somatic alterations account for thedysregulation of dozens of cancer genes and path-ways in each of 14 tumor contexts. To demonstrateproof of concept, we showed that perturbations tar-geting OIP5-AS1 (an inferred tumor suppressor) andTUG1 and WT1-AS (inferred onco-lncRNAs) dysre-gulated cancer genes and altered proliferation ofbreast and gynecologic cancer cells. Our analysis in-dicates that, although most lncRNAs are dysregu-lated in a tumor-specific manner, some, includingOIP5-AS1, TUG1, NEAT1, MEG3, and TSIX, synergis-tically dysregulate cancer pathways in multiple tumorcontexts

Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas

Although theMYConcogene has been implicated incancer, a systematic assessment of alterations ofMYC, related transcription factors, and co-regulatoryproteins, forming the proximal MYC network (PMN),across human cancers is lacking. Using computa-tional approaches, we define genomic and proteo-mic features associated with MYC and the PMNacross the 33 cancers of The Cancer Genome Atlas.Pan-cancer, 28% of all samples had at least one ofthe MYC paralogs amplified. In contrast, the MYCantagonists MGA and MNT were the most frequentlymutated or deleted members, proposing a roleas tumor suppressors.MYCalterations were mutu-ally exclusive withPIK3CA,PTEN,APC,orBRAFalterations, suggesting that MYC is a distinct onco-genic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such asimmune response and growth factor signaling; chro-matin, translation, and DNA replication/repair wereconserved pan-cancer. This analysis reveals insightsinto MYC biology and is a reference for biomarkersand therapeutics for cancers with alterations ofMYC or the PMN

Genomic, Pathway Network, and Immunologic Features Distinguishing Squamous Carcinomas

This integrated, multiplatform PanCancer Atlas study co-mapped and identified distinguishing molecular features of squamous cell carcinomas (SCCs) from five sites associated with smokin