742 research outputs found
Insuficiencia cardíaca diastólica: valoración y ejercicio físico
Pacientes con insuficiencia cardíaca diastólica (ICD) muestran síntomas de insuficiencia cardíaca clásica, sin embargo tienen una función sistólica normal. La intolerancia al ejercicio en ICD debe evaluarse con mediciones que han demostrado prognosis, como consumo de oxígeno pico (VO2pico), umbral ventilatorio (VT), y relación entre ventilación minuto y producción de dióxido de carbono (pendiente VE/VCO2). La prueba de 6 min andando presenta una gran variabilidad y sólo una correlación modesta con VO2pico, y resulta inaceptable cuando son necesarios valores exactos para la estratificación de pacientes. De forma similar a los pacientes con insuficiencia cardíaca sistólica, pacientes ICD presentan valores marcadamente reducidos de VO2pico y VT, y elevada pendiente VE/VCO2. Pacientes ICD han mostrado adaptaciones al entrenamiento incluyendo mejoras en la tolerancia al ejercicio, VO2pico y VT, lo que indica que las terapias que han sido exitosas en insuficiencia cardíaca sistólica podrían tener su papel en ICD
Insuficiència cardíaca diastòlica: valoració i exercici físic
Pacients amb insuficiència cardíaca diastòlica (ICD) mostren símptomes d'insuficiència cardíaca clàssica, per bé que tenen una funció sistòlica normal. La intolerància a l'exercici en ICD s'ha d'avaluar amb mesuraments que han demostrat prognosi, com a consum d'oxigen pic (VO2pic), llindar ventilatori (VT) i relació entre ventilació minut i producció de diòxid de carboni (pendent VE/VCO2). La prova de 6 minuts caminant presenta una gran variabilitat i només una correlació modesta amb VO2pic, i resulta inacceptable quan calen valors exactes per a l'estratificació de pacients. Semblantment, els pacients amb insuficiència cardíaca sistòlica, pacients ICD, presenten valors marcadament reduïts de VO2pic i VT, i elevat pendent VE/VCO2. Pacients ICD han mostrat adaptacions a l'entrenament, incloent-hi millores en la tolerància a l'exercici, VO2pic i VT, la qual cosa indica que les teràpies exitoses en insuficiència cardíaca sistòlica podrien tenir el seu paper en ICD
Padrões de acúmulo de proteínas e carboidratos durante a embriogênese somática de Acca sellowiana
The aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid-Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the somatic embryogenesis' in vitro morphogenesis and differentiation processes, the total protein levels decreased and the soluble sugars levels increased during the first 30 days in culture and remained stable until the 120th day. On the other hand, total protein levels increased according to the progression in the developmental stages of the somatic embryos. The levels of total sugars and starch increased in the heart and cotyledonary stages, and decreased in the torpedo and pre-cotyledonary stages. These compounds play a central role in the development of somatic embryos of Acca sellowiana. © 2009 Embrapa Informação Tecnológica.O objetivo deste trabalho foi quantificar os teores de proteína, amido e açúcares totais durante a histodiferenciação e desenvolvimento dos embriões somáticos em Acca sellowiana Berg. Para as observações histológicas, as amostras foram desidratadas em uma bateria de etanol, emblocadas em historesina e coradas com azul de toluidina (morfologia), azul de coomassie (corpos proteicos) e reativo ácido periódico de Schiff (amido). As proteínas foram extraídas usando uma solução tampão, precipitadas usando etanol e quantificadas por meio do reativo de Bradford. Os açúcares totais foram extraídos usando uma solução metanol-clorofórmioágua (12:5:3) e quantificados pela reação com antrona a 0,2%. O amido foi extraído usando uma solução de ácido perclórico a 30% e quantificado pela reação com antrona a 0,2%. Durante a diferenciação e morfogênese in vitro da embriogênese somática, os teores de proteínas totais decresceram e os açúcares solúveis aumentaram durante os 30 primeiros dias em cultura e permaneceram constantes até os 120 dias. Por outro lado, os teores das proteínas totais apresentaram incremento de acordo com a progressão nos estádios de desenvolvimento dos embriões somáticos. Os teores de açúcares totais e de amido aumentaram nos estádios cordiforme e cotiledonar e diminuíram nos estádios torpedo e pré-cotiledonar. Esses compostos exercem papel central no desenvolvimento de embriões somáticos de Acca sellowiana.Fil: Cangahuala-Inocente, Gabriela Claudia. Universidade Federal de Santa Catarina; BrasilFil: Steiner, Neusa. Universidade Federal de Santa Catarina; BrasilFil: Maldonado, Sara Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Guerra, Miguel Pedro. Universidade Federal de Santa Catarina; Brasi
Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus
Tobacco plants were transformed with the open reading frame 3 gene from Potato virus X (PVX) coding for the p12 protein. Although the transgenic plants exhibited a normal morphological aspect, microscopic examination revealed extensive alterations in leaf tissue structure. After being challenged with PVX, the transgenic plants showed resistance to PVX infection and formation of specific leaf symptoms consisting of concentric rings encircled by necrotic borders. These novel symptoms were accompanied by biochemical changes normally associated with the hypersensitive response (HR) and were absent in noninfected transgenic plants or in PVX-infected nontransgenic plants. No equivalent virus resistance was observed after inoculation with Tobacco mosaic virus or Potato virus Y, suggesting the presence of a specific resistance mechanism. Despite development of HR-like symptoms, systemic acquired resistance was not induced in PVX-infected p12 transgenic plants. No evidence of an RNA-mediated resistance mechanism was found.Fil: Kobayashi, Ken. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Cabral, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Maldonado, Sara Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Mentaberry, Alejandro Nestor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentin
Secretory tissues of the flower of Sanango racemosum (Gesneriaceae). I. Light microscopy
Fil: Maldonado, Sara. Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires; ArgentinaFil: Otegui, Marisa Silvina. Instituto de Fisiología Vegetal (INFIVE). Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; Argentin
Embryological features and bacterial transmission to gynoecium and ovule in Myrsine laetevirens (Myrsinaceae)
Fil: Otegui, Marisa Silvina. Instituto de Fisiología Vegetal (INFIVE). Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Maldonado, Sara. Facultad de Ciencias Exactas y Naturales. Universidad Nacional de Buenos Aires; Argentin
Storage lipids and proteins of Euterpe edulis seeds
Comparative studies on fatty acid and protein composition of the endosperm and embryo of palmito (Euterpe edulis Martius) were conducted using gas-liquid chromatography and sodium dodecyl sulfate–polyacrylamide gel electrophoresis. On a dry weight basis, the embryo contained extremely lower amounts of lipids and proteins than did the endosperm, which was associated with the scarce lipid and protein bodies previously reported in axis and cotyledon. The fatty acid composition also exhibited differences between both tissues: (I) the fatty acid diversity was greater in embryo than in endosperm; (II) embryo and endosperm contained predominantly linoleic, palmitic, oleic and stearic acids even though the relative values were different for each tissue. As compared to other palm species, the higher fatty acid unsaturation in Euterpe edulis seed could be involved in the previously reported short longevity and recalcitrant behavior during storage. Proteins of both tissues were heterogeneous in molecular mass. Some proteins were tissue-specific, but other were common, among them a highly glycosylated protein which migrated at about 55 kDa. We hypothesize that the latter, also reported in all previously studied palm species, is one of the proteins characterizing the Arecaceae family.Fil: Panza, Víctor. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Pighín, Darío Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Lainez, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Pollero, Ricardo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Maldonado, Sara Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentin
Estudio acústico y electroacústico de la sala 18 de Kinépolis (Madrid)
El objetivo de este proyecto se materializa en el diseño acústico y electroacústico de las salas de cine con número 18, 19, 20 y 21 del centro de cines Kinépolis situado en la Ciudad de la Imagen. Dichas salas son idénticas por lo que con el estudio y análisis de una se podrán extrapolar los resultados a las demás, por tanto a partir de ahora se realizará el diseño acústico y electroacústico centrado en la sala 18.
Así pues se procederá a certificar que dicha sala cumple la norma THX. En dicho recinto se habrán aplicando con anterioridad diferentes técnicas de acondicionamiento sonoro (insonorización mediante el uso de materiales tanto absorbentes como reflectantes, arquitectura de la sala ), así como todos los acondicionamientos propios de un espacio de uso público cumpliendo las consiguientes normativas de seguridad.
Se analizará la sala en su estado de partida mediante medidas acústicas in-situ tanto del tiempo de reverberación como del ruido de fondo, así como medidas espaciales con un medidor de alta precisión (láser); para posteriormente realizar las simulaciones en el programa EASE 4.01 de manera que la simulación sea lo más fidedigna posible. Estas medidas se realizarán de acuerdo a lo especificado en la norma UNE-EN ISO 3382.
Cuando el comportamiento acústico del modelado de la sala sea el mismo que el de la sala real (respondan con el mismo Tiempo de Reverberación), mediante la introducción de los materiales adecuados y del ruido de fondo; se procederá al diseño electroacústico del equipo necesario para implementar el sistema de sonido multicanal Dolby (R).
En el estudio electroacústico se distribuirán los diferentes altavoces que correctamente insertados ajustados sus niveles y retardos cubrirán con su señal uniformemente la sala cumpliendo los requisitos de la norma Dolby (R).
Y finalmente una vez propuesto el sistema electroacústico y comprobado su correcto funcionamiento y por tanto el cumplimiento de la norma THX se propondrán mejoras en el sistema.
Hay que señalar que aunque las recomendaciones THX son conocidas como norma THX no es una norma como tal, sino un conjunto de recomendaciones, que aconsejan utilizar una norma u otra para cada requisito. De ahora en adelante se referirá en este documento como norma THX. Se recuerda una vez más que dado que las salas son idénticas, y que las medidas realizadas en cada una de ellas son similares, se procederá en adelante al estudio de la sala única sala, la sala 18, siendo todos los estudios, análisis, comentarios y conclusiones aplicables a las otras 3 salas.
Abstract
The aim of this Project is the acoustic and electroacoustic design of the projection rooms 18th, 19th, 20th and 21st of the theater kinépolis, which is located at “Ciudad de la imagen”. These rooms are equal so with the studing and analazing of one of them , the results can be extrapolted from this one to the others, therefore as from now the and electroacoustic design will focus on the 18th room.
As result the room will be certified that it comply with the standard THX.
The starting point of the room will be analized such on-spot acoustical measures as the reverberation time and the noise level, in the same way the spatial measurements will be make with a precise laser meter, in order to perform simulations with the data obtained with the EASE 4.01 program. These mesurements should be made in accordance with the standard UNE-EN ISO 3382.
Once the acoustical behavior of the simulated room became the same as the real room (the same reverberation times), through the configuration of the suitable materials and the background noise, previously measured, the electroacoustic design of the equipment necessary to develop the multichannel sound system Dolby (R) will be completed.
In the electroacoustic design the sound power level and the dealys of the speakers and its amplifiers will be configurated till amplifiers provide an uniform coverage over the entire room in compliance with the Dolby (R) standard.
Finally, once an electroacoustic system will be proposed and checked it works correctly in compliance with the THX standard, posible improvements will be identified.
Abstract. Acoustic and electroacoustic study of the 18th room of the movie theater Kinépolis (Madrid)
It must however be pointed out that although the THX standard is known as a standard it is actually a set of recommended practices made by Lucasfilm Ltd, anyway, from now on we will call it THX standard
Physical activity on cardiorespiratory fitness and cardiovascular risk in premenopausal and postmenopausal women: a systematic review of randomized controlled trials
Importance: The apparent cardioprotective effects of endogenous estrogens to prevent cardiovascular disease in premenopausal women are reduced with the loss of estrogen post-menopause. Cardiorespiratory fitness and cardiovascular risk factors are closely related to physical activity levels.
Objective: This study conducted a critical assessment of studies for health prevention that analyze the effects of physical activity programs on cardiorespiratory fitness and cardiovascular risk factors in women, comparing premenopausal and postmenopausal states, through a systematic review of randomized controlled trials.
Evidence review: A computerized literature search was performed to include articles up until December 2021 in the following online databases: PubMed, Cochrane, Scopus, SportDiscus, and Web of Science. Regarding physical activity intervention, women of all ages were engaged. The PEDro scale and Oxford's evidence levels were used for the assessment of the risk of bias in the included articles.
Findings: Fourteen scientific articles met the inclusion criteria. Great variability was found in physical activity variables. All the studies found an improvement in at least one variable. The risk of bias was high, with all the articles obtaining a low methodological quality, except two with high methodological quality. Only one article considered the differences in the menopausal state observing the effects of physical activity intervention and highlighting the importance of physical activity in both states.
Conclusions and relevance: To strengthen the evidence for the benefits of physical activity programs in women and to observe the effects depending on their menopausal state, there is an ongoing need for more rigorous randomized controlled trials of appropriate length and dose, with individualized exercise intensity
Influence of the Position on the Bicycle on the Frontal Area in Road Cyclists
The aims of the present study were to determine whether the estimations of the frontal area of the combined cyclist-bicycle
(APCB) obtained with the Heil’s non-logarithmic prediction equations (NPE) in the stem position (SP), brake hoods
position (BHP) and drops position (DP) are comparable to the measured APCB with the computerized planimetry (CP)
method, and to analyse with the CPmethod and the NPE the influence of the body position on the APCB. Nineteen participants
competing in the Spanish Road Cycling First division took part in the study. The NPE overestimated the APCB in
the BHP and in the DP compared with the measured APCB with the CP method (6.9% and 5.1%, respectively; p<0.05).
Significant differences among the three positions were obtained with the CP method. The overestimation of the APCB
with the NPE in the BHP and in the DP, and the less sensitivity of the NPE to show significant differences between the SP
and DP suggest that the NPE are not appropriate to accurately predict the APCB
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