Impressionistic techniques applied in sound art & design

Sound art and design collectively refer to the process of specifying, acquiring, manipulating or generating sonic elements to evoke emotion and environment. Sound is used to convey the intentions, emotions, spirit or aura of a story, performance, or sonic installation. Sound connects unique aural environments, creating an immersive experience via mood and atmosphere. Impressionistic techniques such as Impasto, Pointillism, Sgraffito, Stippling introduced by 19th-century painters captured the essence of their subject in more vivid compositions, exuding authentic movements and atmosphere. This thesis applied impressionistic techniques using sound art and design to project specific mood and atmosphere responses among listeners. Four unique sound textures, each representing a technique from Impressionism, and a fifth composite sound texture were created for this project. All five sound textures were validated as representative of their respective Impressionistic technique. Only sonic Pointillism matched its emotive intent. This outcome supports the research question that sound art and design can be used to direct listeners’ mood and atmosphere responses. Partnering Impressionistic principles with sound art and design offers a deeper palette to sonically deliver more robust, holistic soundscapes for amplifying an audience’s listening experience. This project provides a foundation for future explorations and studies in applying cross-disciplinary artistic techniques with sound art and design or other artistic endeavors

Urban Redevelopment and the Elimination of Blight: A Case Study of Missouri\u27s Chapter 353

The purpose of this Article is to consider carefully whether Chapter 353 was meant to be blight driven; that is, whether the Missouri legislature enacted Chapter 353 to remedy obvious cases of blight, or whether the legislature was equally concerned about promoting economic development within urban areas generally. Part I of the Article describes Chapter 353 and project approval under the statute. Part II examines the criticisms of the statute and several of the studies that have evaluated the effectiveness of Chapter 353. Next, Part III develops a legislative history for Chapter 353 in an effort to discern the objectives of the statute. Part IV of the Article evaluates the use of Chapter 353 in light of the public use doctrine, and Part V evaluates the use of the statute in light of legal challenges to blight determinations. Finally, Part VI of the Article develops conclusions about the proper role of blight determinations in urban renewal programs

Rapid generation of broad T-cell immunity in humans after a single injection of mature dendritic cells

Dendritic cells (DCs) are potent antigen-presenting cells that initiate protective T-cell immunity in mice. To study the immunogenicity of DCs in humans, we injected 9 healthy subjects subcutaneously with a control injection of autologous monocyte-derived, mature DCs, followed 4-6 weeks later by DCs pulsed with keyhole limpet hemocyanin (KLH), HLA-A*0201- positive restricted influenza matrix peptide (MP), and tetanus toxoid (TT). Four more subjects received these antigens without DCs. Injection of unpulsed DCs, or antigens alone, failed to immunize. Priming of CD4+ T cells to KLH was observed in all 9 subjects injected with KLH-pulsed DCs, and boosting of TT-specific T-cell immunity was seen in 5 of 6 subjects injected with TT- pulsed DCs. Injection of antigen-pulsed DCs led to a severalfold increase in freshly isolated MP-specific, IFN-γ-secreting CD8+ T cells in all 6 HLA- A*0201-positive subjects, as early as 7 days after injection. When T cells were boosted in culture, there was an increase in MHC tetramer-binding cells and cytotoxic T cells after DC vaccination. These data provide the first controlled evidence of the immunogenicity of DCs in humans, and demonstrate that a single injection of mature DCs rapidly expands T-cell immunity

Technological advancements and their importance for nematode identification

Nematodes represent a species-rich and morphologically diverse group of metazoans known to inhabit both aquatic and terrestrial environments. Their role as biological indicators and as key players in nutrient cycling has been well documented. Some plant-parasitic species are also known to cause significant losses to crop production. In spite of this, there still exists a huge gap in our knowledge of their diversity due to the enormity of time and expertise often involved in characterising species using phenotypic features. Molecular methodology provides useful means of complementing the limited number of reliable diagnostic characters available for morphology-based identification. We discuss herein some of the limitations of traditional taxonomy and how molecular methodologies, especially the use of high-throughput sequencing, have assisted in carrying out large-scale nematode community studies and characterisation of phytonematodes through rapid identification of multiple taxa. We also provide brief descriptions of some the current and almost-outdated high-throughput sequencing platforms and their applications in both plant nematology and soil ecology

Experiment K-6-16. Morphological examination of rat testes. The effect of Cosmos 1887 flight on spermatogonial population and testosterone level in rat testes

Testes from rats flown on Cosmos 1887 for twelve and a half days were compared to basal control, synchronous control and vivarium maintained rats. When the mean weights of flight testes, normalized for weight/100 gms, were compared to the vivarium controls they were 6.7 percent lighter. Although the flight testes were lighter than the synchronous, the difference is not significant. Counts of spermatogonial cells from 5 animals in each group revealed a 4 percent decrease in flight compared to vivarium controls. In both cases the t-Test significance was less than 0.02. The serum testosterone levels of all animals (flight, synchronous and vivarium) were significantly below the basal controls

Exercise training-associated differences in circulating microRNAs and serum-induced endothelial cell migration rate

Cardiovascular Diseases (CVDs) are the primary source of global mortality and morbidity. The initial steps of CVD development occur in the endothelium. MicroRNAs (miRNAs) have recently emerged as novel regulators of cardiovascular physiology and pathology. Interestingly, regular aerobic exercise acts to prevent CVDs and also regulates miRNAs in the circulation (ci-miRNAs). The purpose of this study was to determine the effects of serum from highly active and sedentary, young, healthy individuals on migration rate of endothelial cells in vitro. Secondarily, CVD-associated ci-miRNAs in serum were compared between groups. The results of this study represent a novel way by which sedentary behavior may act as an early risk for CVD development before the appearance of other classic risk factors. RT-qPCR array analysis identified nine ci-miRNAs as > 4-fold differentially expressed in serum of trained versus inactive subjects, though more subjects are needed before any conclusions about ci-miRNA differences can be made

Markers of Mitochondrial Mitophagy and Fusion - to - Fission Ratio are Greater in Older vs. Young Rats

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Mutant Huntingtin Fragments Form Oligomers in a Polyglutamine Length-Dependent Manner \u3cem\u3ein Vitro\u3c/em\u3e and \u3cem\u3ein Vivo\u3c/em\u3e

Huntington disease (HD) is caused by an expansion of more than 35–40 polyglutamine (polyQ) repeats in the huntingtin (htt) protein, resulting in accumulation of inclusion bodies containing fibrillar deposits of mutant htt fragments. Intriguingly, polyQ length is directly proportional to the propensity for htt to form fibrils and the severity of HD and is inversely correlated with age of onset. Although the structural basis for htt toxicity is unclear, the formation, abundance, and/or persistence of toxic conformers mediating neuronal dysfunction and degeneration in HD must also depend on polyQ length. Here we used atomic force microscopy to demonstrate mutant htt fragments and synthetic polyQ peptides form oligomers in a polyQ length-dependent manner. By time-lapse atomic force microscopy, oligomers form before fibrils, are transient in nature, and are occasionally direct precursors to fibrils. However, the vast majority of fibrils appear to form by monomer addition coinciding with the disappearance of oligomers. Thus, oligomers must undergo a major structural transition preceding fibril formation. In an immortalized striatal cell line and in brain homogenates from a mouse model of HD, a mutant htt fragment formed oligomers in a polyQ length-dependent manner that were similar in size to those formed in vitro, although these structures accumulated over time in vivo. Finally, using immunoelectron microscopy, we detected oligomeric-like structures in human HD brains. These results demonstrate that oligomer formation by a mutant htt fragment is strongly polyQ length-dependent in vitro and in vivo, consistent with a causative role for these structures, or subsets of these structures, in HD pathogenesis