Assédio Moral E Sexual: À Luz Do Direito Do Trabalho E Direito Penal E As Consequências No Ordenamento Jurídico Brasileiro

A nalyze the legal consequences of bullying and sexual harassment in the scope ofLabor Law, Brazilian Criminal Law and society. Besides the measures adopted by theauthorities and companies against the aggressors and the way of restructuring of theseindividuals in the relation of employment and citizenship. It is understood relevant to addressthe psychological reorganization of victims. The methodology develope d is thebibliographical compilation and the study of the Brazilian Legislation. At the outset, it isimportant to note the historical evolution of the occurrence of moral harassment fromantiquity to the present day, in a simplified overview, evidencing i ts concepts andconsequences, as well as the requirements for its consummation. Then a brief analysis ofbullying and sexual harassment and their ratings. Afterwards, it will be highlighted themismatch of power and procedural character in Brazilian law, d iagnosing the rights, giventheir vulnerability and hyposufficiency in the application in light of the Brazilian CriminalCode 1940 (CPB), Consolidation of Brazilian Labor Laws 2017 (CLTB) and Constitution –1988 (CFB), among other legal norms. Finally , the examining concepts of Public Policiesand Principles regarding physical, sexual and verbal violence, as well as psychologicaldegradation will be expressed.Objetiva se analisar as consequências jurídicas do assédio moral e assédio sexual noâmbito do direito do trabalho, orden amento jurídico penal brasileiro e sociedade. Além dasmedidas adotadas pelas autoridades e empresas contra os agressores e o modo dereestruturação destes indivíduos na relação de emprego e cidadania. Entende se relevanteabordar sobre a reorganização psi cológica das vítimas. A metodologia desenvolvida é acompilação bibliográfica e o estudo da Legislação Brasileira. Ressalta se, de início, aevolução histórica sobre o surgimento do assédio moral desde à antiguidade aos dias atuais,de modo simplificado, e videnciando se seus conceitos e consequências, bem como osrequisitos para sua consumação. Em seguida, uma breve análise do assédio moral e assédiosexual e suas classificações. Após, será salientado sobre o desiquilíbrio do Poder e CaráterProcessual na L egislação Brasileira, diagnosticando os direitos, ante sua vulnerabilidade ehipossuficiência na aplicação à luz do Código Penal Brasileiro 1940 (CPB), Consolidaçãodas Leis do Trabalho Brasileira 2017 (CLTB) e Constituição Federal Brasileira 1988(C FB), entre outras normas legais. Por fim, será expresso os conceitos examinadores dasPolíticas Públicas e Princípios no que tange às violências físicas, sexuais e verbais, além dasdegradações psicológicas

Construction and sequence sampling of deep-coverage, large-insert BAC libraries for three model lepidopteran species

<p>Abstract</p> <p>Background</p> <p><it>Manduca sexta, Heliothis virescens</it>, and <it>Heliconius erato </it>represent three widely-used insect model species for genomic and fundamental studies in Lepidoptera. Large-insert BAC libraries of these insects are critical resources for many molecular studies, including physical mapping and genome sequencing, but not available to date.</p> <p>Results</p> <p>We report the construction and characterization of six large-insert BAC libraries for the three species and sampling sequence analysis of the genomes. The six BAC libraries were constructed with two restriction enzymes, two libraries for each species, and each has an average clone insert size ranging from 152–175 kb. We estimated that the genome coverage of each library ranged from 6–9 ×, with the two combined libraries of each species being equivalent to 13.0–16.3 × haploid genomes. The genome coverage, quality and utility of the libraries were further confirmed by library screening using 6~8 putative single-copy probes. To provide a first glimpse into these genomes, we sequenced and analyzed the BAC ends of ~200 clones randomly selected from the libraries of each species. The data revealed that the genomes are AT-rich, contain relatively small fractions of repeat elements with a majority belonging to the category of low complexity repeats, and are more abundant in retro-elements than DNA transposons. Among the species, the <it>H. erato </it>genome is somewhat more abundant in repeat elements and simple repeats than those of <it>M. sexta </it>and <it>H. virescens</it>. The BLAST analysis of the BAC end sequences suggested that the evolution of the three genomes is widely varied, with the genome of <it>H. virescens </it>being the most conserved as a typical lepidopteran, whereas both genomes of <it>H. erato </it>and <it>M. sexta </it>appear to have evolved significantly, resulting in a higher level of species- or evolutionary lineage-specific sequences.</p> <p>Conclusion</p> <p>The high-quality and large-insert BAC libraries of the insects, together with the identified BACs containing genes of interest, provide valuable information, resources and tools for comprehensive understanding and studies of the insect genomes and for addressing many fundamental questions in Lepidoptera. The sample of the genomic sequences provides the first insight into the constitution and evolution of the insect genomes.</p

Fire weather risk analysis over Portugal in the last decades and their impacts over the atmosphere - The Monchique study case

More intense fire seasons have been favoured by climate changes worldwide, like Russia, Brazil, the USA, Canada and Portugal. Portugal experienced numerous severe fire seasons with catastrophic wildfires that caused enormous impacts in the last years. This study aimed to investigate the fire risk evolution in Portugal over the last 40 years and the extreme wildfire emission impacts derived from remote sensing data. First, the Fire Weather Index (FWI) from 1979 to 2020, at 0.25º spatial resolution, provided by the European Centre for Medium-Range Weather Forecasts (ECMWF) ERA5 reanalysis version 4 based on meteorological variables, was used. Then, FWI monthly mean values and trends were analysed for four districts of Southern Portugal (Beja, Evora, Faro and Portalegre). The results indicate that the Faro district presented extreme fire risk values, which peaked on August 2, 2018, one day before the Monchique (a mountain in Faro) wildfire began and lasted between August 3 and 10. The Monchique wildfire was the most destructive in Portugal during 2018, with almost 27.000 ha burned. Second, based on the previous results, atmospheric products derived from the TROPOspheric Monitoring Instrument (TROPOMI) aboard the Sentinel-5 Precursor satellite, the first Copernicus mission dedicated to atmospheric composition monitoring, were collected. These datasets were obtained from Google Earth Engine (GEE), the online platform that combines multiple imageries and datasets with cloud processing to perform analyses. The Carbon monoxide (CO) and Nitrogen dioxide (NO2) concentrations, as well as Absorbing Aerosol Index (AAI) products were analysed during the fire event. The concentrations released by the wildfire reached values 3 and 5 times higher than usual for CO and NO2, respectively. Therefore, the work confirms that extreme wildfire events can release huge pollutant concentrations into the atmosphere. Also, the Sentinel-5 products are useful to evaluate the fire emission evolution in extreme wildfires events and may constitute additional valuable information to combine with ground-based information to map air quality related to wildfire occurrences

Experimental study of histological changes in vascular loops according to the duration of the postoperative period: Application in reconstructive microsurgery

OBJECTIVES: To analyze the histological changes observed in venous grafts subjected to arterial blood flow as a function of the duration of the postoperative period to optimize their use in free flap reconstructions. METHOD: Twenty-five rats (7 females and 18 males) underwent surgery. Surgeries were performed on one animal per week. Five weeks after the first surgery, the same five animals were subjected to an additional surgery to assess the presence or absence of blood flow through the vascular loop, and samples were collected for histological analysis. This cycle was performed five times. RESULTS: Of the rats euthanized four to five weeks after the first surgery, no blood flow was observed through the graft in 80% of the cases. In the group euthanized three weeks after the first surgery, no blood flow was observed in 20% of the cases. In the groups euthanized one to two weeks after the first surgery, blood flow through the vascular loop was observed in all animals. Moreover, intimal proliferation tended to increase with the duration of the postoperative period. Two weeks after surgery, intimal proliferation increased slightly, whereas strong intimal proliferation was observed in all rats evaluated five weeks after surgery. CONCLUSION: Intimal proliferation was the most significant change noted in venous grafts as a function of the duration of the postoperative period and was directly correlated with graft occlusion. In cases in which vascular loops are required during free flap reconstruction, both procedures should preferably be performed during the same surgery

Erythrina velutina Willd. alkaloids : piecing biosynthesis together from transcriptome analysis and metabolite profiling of seeds and leaves

Introduction: Natural products of pharmaceutical interest often do not reach the drug market due to the associated low yields and difficult extraction. Knowledge of biosynthetic pathways is a key element in the development of biotechnological strategies for plant specialized metabolite production. Erythrina species are mainly used as central nervous system depressants in folk medicine and are important sources of bioactive tetracyclic benzylisoquinoline alkaloids (BIAs), which can act on several pathology-related biological targets. Objectives: In this sense, in an unprecedented approach used with a non-model Fabaceae species grown in its unique arid natural habitat, a combined transcriptome and metabolome analyses (seeds and leaves) is presented. Methods: The Next Generation Sequencing-based transcriptome (de novo RNA sequencing) was carried out in a NextSeq 500 platform. Regarding metabolite profiling, the High-resolution Liquid Chromatography was coupled to DAD and a micrOTOF-QII mass spectrometer by using electrospray ionization (ESI) and Time of Flight (TOF) analyzer. The tandem MS/MS data were processed and analyzed through Molecular Networking approach. Results: This detailed macro and micromolecular approach applied to seeds and leaves of E. velutina revealed 42 alkaloids, several of them unique. Based on the combined evidence, 24 gene candidates were put together in a putative pathway leading to the singular alkaloid diversity of this species. Conclusion: Overall, these results could contribute by indicating potential biotechnological targets formodulation of erythrina alkaloids biosynthesis as well as improve molecular databases with omic data from a non-model medicinal plant, and reveal an interesting chemical diversity of Erythrina BIA harvested in Caatinga