Antiviral Activity Of Bacillus Sp. Isolated From The Marine Sponge Petromica Citrina Against Bovine Viral Diarrhea Virus, A Surrogate Model Of The Hepatitis C Virus

The Hepatitis C virus causes chronic infections in humans, which can develop to liver cirrhosis and hepatocellular carcinoma. The Bovine viral diarrhea virus is used as a surrogate model for antiviral assays for the HCV. From marine invertebrates and microorganisms isolated from them, extracts were prepared for assessment of their possible antiviral activity. Of the 128 tested, 2 were considered active and 1 was considered promising. The best result was obtained from the extracts produced from the Bacillus sp. isolated from the sponge Petromica citrina. The extracts 555 (500 μg/mL, SI>18) and 584 (150 μg/mL, SI 27) showed a percentage of protection of 98% against BVDV, and the extract 616, 90% of protection. All of them showed activity during the viral adsorption. Thus, various substances are active on these studied organisms and may lead to the development of drugs which ensure an alternative therapy for the treatment of hepatitis C. © 2013 by the authors; licensee MDPI, Basel, Switzerland.5512191230Yasuhara-Bell, J., Yang, Y., Barlow, R., Trapido-Rosental, H., Lu, Y., In vitro evaluation of marine microorganism extracts for antiviral activity (2010) Virol. J., 7, p. 182Ravikumar, Y.S., Upasana, R., Nandhitha, M., Perween, A., Naika, H.R., Inhibition of hepatitis C virus replication by herbal extract: Phyllanthus amarus as a potent natural source (2011) Virus Res., 158, pp. 89-97Li, H., Stoddard, M.B., Wang, S., Blair, L.M., Giorgi, E.E., Elucidation of Hepatitis C Virus Transmission and Early Diversification by Single Genome sequencing (2012) PLoS Pathog., 8, pp. e1002880Suzuki, T., Ishii, K., Aizaki, H., Wakita, T., Hepatitis C viral life cycle (2007) Adv. Drug Del. Rev., 59, pp. 1200-1212Barnes, E., Folgori, A., Capone, S., Swadling, L., Aston, S., Kurioka, A., Meyer, J., Klenerman, P., Novel adenovirus-based vaccines induce broad and sustained T cell responses to HCV in man (2012) SciTransl Med., 4, pp. 115ra1. , doi:10.1126/scitranslmed.3003155Sako, K., Aoyama, H., Sato, S., Hashimoto, Y., Baba, M., γ-Carboline derivatives with anti-bovine viral diarrhea virus (BVDV) activity (2008) Bioorg. Med. Chem., 16, pp. 3780-3790Martinot-Peignoux, M., Boyer, N., Pouteau, M., Castelnau, C., Giuily, N., Duchatelle, V., Aupérin, A., Erlinger, S., Predictors of sustained response to alpha interferon therapy in chronic hepatitis C (1998) J. Hepatol., 29, pp. 214-223Lemon, S.M., McKeating, J.A., Pietschmann, T., Frick, D.N., Glenn, J.S., Tellinghuisen, T.L., Symons, J., Furman, P.A., Development of novel therapies for hepatitis C (2010) Antiviral Res., 86, pp. 79-92Duverlie, G., Wychowski, C., Cell culture systems for the hepatitis C virus (2007) J. Gastroenterol., 13, pp. 2442-2445Finkielsztein, L.M., Moltrasio, G.Y., Caputto, M.E., Castro, E.F., Cavallaro, L.V., Moglioni, A.G., What is known about the antiviral agents active against bovine viral diarrhea virus (BVDV)? (2010) Curr. Med. Chem., 17, pp. 2933-2955Buckwold, V.E., Beer, B.E., Donis, R.O., Bovine viral diarrhea virus as a surrogate model of hepatitis C virus for the evaluation of antiviral agents (2003) Antiviral Res., 60, pp. 1-15Zitzmann, N., Mehta, A.S., Carrouee, S., Butters, T.D., Platt, F.M., McCauley, J., Blumberg, B.S., Block, T.M., Imino sugars inhibit the formation and secretion of bovine viral diarrhea virus, a Pestivirus model of hepatitis C virus: Implications for the development of broad spectrum anti-hepatitis virus agents (1999) Proc. Natl. Acad. Sci., 96, pp. 11878-11882Baginski, S.G., Pevear, D.C., Seipel, M., Sun, S.C., Benetatos, C.A., Chunduru, S.K., Rice, C.M., Collett, M.S., Mechanism of action of a pestivirus antiviral compound (2000) Proc. Natl. Acad. Sci., 97, pp. 7981-7986Buckwold, V.E., Wei, J., Wenzel-Mathers, M., Russell, J., Synergistic in vitro interactions between alpha interferon and ribavirin against bovine viral diarrhea virus and yellow fever virus as surrogate models of hepatitis C virus replication (2003) Antimicrobial. Agents Chemother., 47, pp. 2293-2298Yanagida, K., Baba, C., Baba, M., Inhibition of bovine viral diarrhea virus (BVDV) by mizoribine: Synergistic effect of combination with interferon-α (2004) Antiviral Res., 64, pp. 195-201Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays (1983) J. Immun. Meth., 65, pp. 55-63Scudiero, D.A., Shoemaker, R.H., Paull, K.D., Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines (1988) Cancer Res., 48, pp. 4827-4833Reed, L.J., Münch, H.A., A simple method of estimating fifty percent endpoints (1938) Am. J. Hyg., 27, pp. 493-497Takeuchi, H., Baba, M., Shigeta, S., An application of tetrazolium (MTT) colorimetric assay for the screening of anti-herpes simplex virus compounds (1991) J. Virol. Methods., 33, pp. 61-71Koseki, I., Simoni, I.C., Nakamura, I.T., Noronha, A.B., Costa, S.S., Antiviral activity of plant extracts against aphtovirus, pseudorabiesvírus and pestivirus in cell cultures (1990) Microbios. Letters., 44, pp. 19-30Pospiech, A., Neumann, B., A versatile quick-prep of genomic DNA from Gram-positive bacteria (1995) Trends Genetics, 11, pp. 217-218Lane, D.J., Pace, B., Olsen, G.J., Stahl, D.A., Sogin, M.L., Pace, N.R., Rapid determination of 16S ribosomal RNA sequences for phylogenetic analysis (1985) Proc. Nat. Acad. Sci., 82, pp. 6955-6959Menezes, C.B.A., Bonugli-Santos, R.C., Miqueletto, P.B., Passarini, M.R.Z., Silva, C.H.D., Justo, M.R., Rebeca, R., Sette, L.D., Microbial diversity associated with algae, ascidians and sponges from the north coast of São Paulo state, Brazil (2010) Microbiol. Res., 165, pp. 466-482Costa-Lotufo, L.V., Wilke, D.V., Jimenez, P.C., Epifanio, R.A., Organismos marinhos como fonte de novos fármacos: Histórico & perspectivas (2009) Quim. Nova., 32, pp. 703-716Laport, M.S., Santos, O.C., Muricy, G., Marine sponges: Potential sources of new antimicrobial drugs (2009) Curr. Pharm. Biotechnol., 10, pp. 86-105Marinho, P.R., Muricy, G.R.S., Silva, M.F.L., de Marval, M.G., Laport, M.S., Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges (2010) Brazilian J. Pharmac., 20, pp. 267-275Donadio, S., Monciardini, P., Sosio, M., Polyketide synthases and nonribosomal peptide synthetases: The emerging view from bacterial genomics (2007) Nat. Prod. Rep., 24, pp. 1073-1109Mikhaǐlova, N.A., Nagieva, F.G., Grin'ko, O.M., Zverev, V.V., Experimental study of antiviral activity of spore-forming bacterium Bacillus pumilus "Pashkov" (2010) Mikrobiol. Epidemiol. Immunobiol., 2, pp. 69-74Gribencha, S.V., Potselueva, L.A., Barinskiǐ, I.F., Deev, S.M., Balandin, T.G., Leshchinskaia, I.B., Antiviral activity of Bacillus intermedius RNAase in guinea-pigs and rabbits infected with outdoor rabies virus (2006) Vopr. Virusol., 51, pp. 41-43Gribencha, S.V., Potselueva, L.A., Barinskiǐ, I.F., Balandin, T.G., Deev, S.M., Leshchinskaia, I.B., The antiviral activity of RNAse Bacillus intermedius in experiments with mice preinfected with street rabies virus (2004) Vopr. Virusol., 49, pp. 38-41Zhou, W.W., Niu, T.G., Purification and some properties of an extracellular ribonuclease with antiviral activity against tobacco mosaic virus from Bacillus cereus (2009) Biotechnol. Lett., 31, pp. 101-105Boya, C.A., Herrera, L., Guzman, H.M., Gutierrez, M., Antiplasmodial activity of bacilosarcinA isolated from the octocoral-associated bacterium Bacillus sp. collected in Panama (2012) J. Pharm. Bioallied. Sci., 4, pp. 66-69El-Sersy, N.A., Abdelwahab, A.E., Abouelkhiir, S.S., Antibacterial and anticancer activity of ε-poly-L-lysine (ε-PL) produced by a marine Bacillus subtilis sp (2012) J. Basic Microbiol., 52, pp. 513-522Craczyc, K., Trojanowska, K., Mueller, A., Antifungal activity of Bacillus coagulans against Fusarium sp (2002) Acta Microbiol. Pol., 51, pp. 275-283Sohn, M.J., Zheng, C.J., Kim, W.G., Macrolactin S, a new antibacterial agent with FabG-inhibitory activity from Bacillus sp. AT28 (2008) J. Antibiot., 61, pp. 687-691Moshafi, M.H., Forootanfar, H., Ameri, A., Antimicrobial activity of Bacillus sp. strain FAS1 isolated from soil. Pak (2011) J. Pharm. Sci., 24, pp. 269-27

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time, and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space. While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes, vast areas of the tropics remain understudied. In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity, but it remains among the least known forests in America and is often underrepresented in biodiversity databases. To worsen this situation, human-induced modifications may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge, it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Epiderme dos segmentos foliares de Mauritia flexuosa L. f. (Arecaceae) em três fases de desenvolvimento Epidermis of leaf segments from Mauritia flexuosaL. f. (Arecaceae) on three phases of development

São apresentados os dados anatômicos da epiderme dos segmentos foliares de Mauritia flexuosa L. f. (Arecaceae) em três fases do desenvolvimento. Os segmentos foliares foram analisados em toda a extensão do limbo. As células intercostais da epiderme das faces adaxial e abaxial evidenciam-se com paredes sinuosas, retangulares e orientadas longitudinalmente em relação ao eixo do segmento foliar com acentuada presença de corpos de sílica esférico-espinulosos. As células epidérmicas da região costal de ambas as faces apresentam paredes retas e variam entre curtas, longas e arredondadas. Os tricomas são simples, unicelulares, longos, com base mais alargada. Os segmentos foliares de M. flexuosa são anfiestomáticos com estômatos tetracíticos. Em secção transversal a epiderme foliar é uniestratificada com câmara subestomática ampla. Os resultados obtidos não demonstraram variações expressivas entre as três fases de desenvolvimento e os caracteres encontrados parecem ser comuns a outras palmeiras.<br>Anatomic data on the epidermis leaf segments from Mauritia flexuosa L. f. (Arecaceae) are presented on three phases of development. Leaf segments were analyzed on the all extension of leaf. Both adaxial and abaxial epidermal cells stand out with sinuous walls, rectangular and longitudinally oriented to the foliar axis with the marked presence of spherical- spiny silica bodies. The back epidermal cells of both surfaces present straight walls and vary among short, long and round. Trichomes are unicellular, simple, long, with a wider base. Leaf segments from M. flexuosa are anphistomatic with tetracitic type stomats. In a cross-section the leaf skin is unistratified with a broad substimatic chamber. The findings obtained showed no significant variations among the three phases of development and the characters that were found appear to be common on other palm trees

Nematóides do Brasil. Parte V: nematóides de mamíferos Brazillan nematodes. Part V: nematodes of mammals

<abstract language="eng">A survey of nematode species parasitizing Brazilian mammals is presented, with enough data to provide their specific identification. The tirst section refers to the survey ofthe species, related to 21 superfamilies, 45 families, 160 genera and 495 species that are illustrated and measurement tables are given. The second section is concerned to the catalogue ofhost mammals which includes 34 families, 176 species and their respective parasite nematodes. The identification of these helminths is achieved by means of keys to the superfamilies, families and genera. Specific determination is induced through the figures and tables as above mentioned

Brazilian Flora 2020: Leveraging the power of a collaborative scientific network

International audienceThe shortage of reliable primary taxonomic data limits the description of biological taxa and the understanding of biodiversity patterns and processes, complicating biogeographical, ecological, and evolutionary studies. This deficit creates a significant taxonomic impediment to biodiversity research and conservation planning. The taxonomic impediment and the biodiversity crisis are widely recognized, highlighting the urgent need for reliable taxonomic data. Over the past decade, numerous countries worldwide have devoted considerable effort to Target 1 of the Global Strategy for Plant Conservation (GSPC), which called for the preparation of a working list of all known plant species by 2010 and an online world Flora by 2020. Brazil is a megadiverse country, home to more of the world's known plant species than any other country. Despite that, Flora Brasiliensis, concluded in 1906, was the last comprehensive treatment of the Brazilian flora. The lack of accurate estimates of the number of species of algae, fungi, and plants occurring in Brazil contributes to the prevailing taxonomic impediment and delays progress towards the GSPC targets. Over the past 12 years, a legion of taxonomists motivated to meet Target 1 of the GSPC, worked together to gather and integrate knowledge on the algal, plant, and fungal diversity of Brazil. Overall, a team of about 980 taxonomists joined efforts in a highly collaborative project that used cybertaxonomy to prepare an updated Flora of Brazil, showing the power of scientific collaboration to reach ambitious goals. This paper presents an overview of the Brazilian Flora 2020 and provides taxonomic and spatial updates on the algae, fungi, and plants found in one of the world's most biodiverse countries. We further identify collection gaps and summarize future goals that extend beyond 2020. Our results show that Brazil is home to 46,975 native species of algae, fungi, and plants, of which 19,669 are endemic to the country. The data compiled to date suggests that the Atlantic Rainforest might be the most diverse Brazilian domain for all plant groups except gymnosperms, which are most diverse in the Amazon. However, scientific knowledge of Brazilian diversity is still unequally distributed, with the Atlantic Rainforest and the Cerrado being the most intensively sampled and studied biomes in the country. In times of “scientific reductionism”, with botanical and mycological sciences suffering pervasive depreciation in recent decades, the first online Flora of Brazil 2020 significantly enhanced the quality and quantity of taxonomic data available for algae, fungi, and plants from Brazil. This project also made all the information freely available online, providing a firm foundation for future research and for the management, conservation, and sustainable use of the Brazilian funga and flora