27 research outputs found
Digestible lysine effects on gene expression by Japanese quails in the pre-laying phase
This study aimed to determine the effects of digestible lysine levels in the diets of Japanese quail (Coturnix coturnix japonica) on performance, blood parameters and the expression of insulin-like growth factor I, and growth hormone receptor (GHR), apolipoprotein A-I (APOA-I), acetyl-CoA-carboxylase (ACC), and fatty acid synthase (FAS) genes. A total of 288 seven-day-old female Japanese quails were randomly assigned to one of three diets that contained 0.8%, 1.10%, or 1.40% digestible lysine. The birds were slaughtered at 42 days old, and relative gene expression was evaluated in the liver by qRT-PCR using the 2-ÎCT method. Lysine supplementation had no effect on weight gain and feed conversion. Abdominal fat was lower in birds supplemented with 0.8% digestible lysine than those supplemented with 1.10% and 1.40%. Increased total cholesterol and triglycerides were elevated in quails that received supplementation of 1.10% digestible lysine compared with the other diets. High density lipoproteins were decreased in birds that received 0.8% digestible lysine. Quails fed with 1.40% digestible lysine had greater expression of GHR and APOA-I than quails fed diets with 0.8 and 1.10% (P <0.05). The greatest expressions of ACC and FAS were observed in the liver of quails fed with 0.8% digestible lysine. The current results suggest that lysine supplementation in the pre-laying phase allows birds to deposit muscle mass to reach the optimal conformation and body fatness that provides an energetic reserve for the productive phase by modulating the expression of genes related to growth and lipid metabolism.
Keywords: Coturnix coturnix japonica, growth, growth hormone, lipid synthesis, lipid metabolis
Chronic lead intoxication in a jaguar (Panthera onca) shot with round lead pellets - case report
ABSTRACT Lead is a heavy metal and an important cause of acute or chronic toxicosis in humans, domestic, and wild animals. This report aims to describe a case of chronic lead poisoning in a jaguar (Panthera onca) kept under human care that was rescued from the wild environment. The animal was rescued in poor condition in 2004 and kept under human care at the Belo Horizonte Zoological Garden (Minas Gerais, Brazil) until 2020, when it presented with anorexia, vomiting and ataxia. Over the past years the animal had episodes of anemia and increased serum urea and creatinine. Radiography demonstrated 21 radiopaque projectiles on the right side of the face. At necropsy there were multiple projectiles surrounded by fibrous tissue in the subcutaneous of the right side of the face, fibrinous peritonitis, multiple gastric ulcers, and melena. The lead dosage was performed using the atomic absorption spectrometry technique using renal tissue collected at necropsy, with a result of 908 ppb (”g/kg). The findings of projectiles associated with the dosage of lead above the reference limits allow the diagnosis of chronic intoxication in this case
Performance and carcass traits of Santa InĂȘs lambs finished with different sources of forage
CaracterĂsticas de crescimento, composição quĂmica, fĂsica e estimativa de massa seca de madeira em clones e espĂ©cies de Eucalyptus jovens
EFFECT OF PLANTING AGE AND SPACING ON ENERGY PROPERTIES OF Eucalyptus grandis W. Hill EX Maiden
Pervasive gaps in Amazonian ecological research
Biodiversity loss is one of the main challenges of our time, and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space. While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes, vast areas of the tropics remain understudied. In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity, but it remains among the least known forests in America and is often underrepresented in biodiversity databases. To worsen this situation, human-induced modifications may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge, it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%â18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost
Antioxidant activity and duodenum transporter gene expression in quail fed citric acid
The study aimed to evaluate the effects of citric acid supplementation on the performance and the gene expression in the duodenum of Japanese quail. Genes related to antioxidant activity: superoxide dismutase (SOD) and glutathione peroxidase 7 (GPX7); and genes related to nutrient uptake in the enterocytesâ apical membrane: sodium-glucose cotransporter 1 (SGLT1, responsible for the glucose absorption), glucose transporter 2 (GLUT2) and B0AT1 (responsible for the absorption of neutral amino acids in brush board membrane) were evaluated. For this, 270 laying quails (Coturnix japonica) were fed a diet supplemented with 0%, 0.6% or 1.2% of citric acid. Gene expression was evaluated by the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) at 35 days old (n = 5). The highest expression of the SGLT1 gene was observed in quails fed the 1.2% citric acid supplemented diet (0.120 AU). Birds fed diets with both levels of citric acid supplementation showed a higher B0AT1 gene expression than quails fed no citric acid. Quails fed the 1.2% of citric acid supplemented diet had the lowest SOD gene expression (0.3455 AU). The birds receiving the diets supplemented with citric acid showed a lower GPX7 gene expression than the quails fed the 0% citric acid diet. These results suggests that the effect of citric acid on gene expression in the digestive tract may have contributed to the greater weight gain and lower feed intake observed in birds fed diets supplemented with citric acid.Keywords: B0AT1, intestinal health, organic acid, pathogenic microorganisms, SGLT
Physical, physiological and anatomical changes in Erythrina speciosa Andrews seeds from different seasons related to the dormancy degree
Host Plant Records for Fruit Flies (Diptera: Tephritidae: Dacini) in the Pacific Islands: 2. Infestation Statistics on Economic Hosts
Detailed host records are listed for 39 species of Bactrocera and 2 species of Dacus fruit flies, infesting 98 species of commercial and edible fruits in the Pacific Island Countries and Territories, based on sampling and incubating in laboratory almost 13,000 field collected samples, or over 380,000 fruits. For each host-fly-country association, quantitative data are presented on the weight and number of fruits collected, the proportion of infested samples, the number of adult flies emerged per kg of fruits and, whenever available, the percentage of individual fruits infested. All the published records of each fly-host-country association are cited and erroneous or dubious published records are rectified or commented. Laboratory forced infestation data are also cited and reviewed