VIVÊNCIAS ACADÊMICAS E REPERCUSSÕES NA QUALIDADE DE VIDA DE GRADUANDOS DE ENFERMAGEM EM UNIVERSIDADE PÚBLICA

Quality of Life (QoL) is related to the expanded concept of health and well-being. The democratization of Higher Education has enabled a greater insertion of young people in universities, generating the need for more research on the academic repercussions on their QoL. This theme assumes relevance when we deal with graduations in nursing, as these professionals will constitute the largest workforce in the health area. Aimed to understand the perception of nursing students in the relationship between academic experiences and quality of life. It was field research, exploratory, quali-quantitative, carried out with 37 university students of the nursing course at a public university in the state of Bahia, approved by the Research Ethics Committee. A semi-structured questionnaire was used to trace the sociodemographic profile and academic characterization, the validated instrument for Quality of Life Assessment: "The World Health Organization Quality of Life-BREF" (WHOQOL-bref), and two open questions for the free expression of students' perception of the relationship between academic experiences and quality of life. The results of the study revealed that the sample profile was mostly female, self-declared black, aged between 20 and 25 years, mostly from the interior of the state, with supplementary income from student scholarships and non-mester students. The most impaired QoL domain was physical and environmental. The personal and study dimension of academic experiences were the most affected. It is necessary to give students a voice and develop institutional strategies and public policies for university education and permanence, resulting in a better QoL of university students.La Calidad de Vida (CV) se relaciona con el concepto ampliado de salud y bienestar. La democratización de la Educación Superior posibilitó una mayor inclusión de los jóvenes en las universidades, generando la necesidad de investigaciones sobre el tema. La relevancia de este estudio se explica porque los enfermeros constituyen una gran fuerza de trabajo en el área de la salud. El objetivo fue comprender la percepción de los académicos sobre la relación entre las experiencias académicas y la CV. Investigación de campo, exploratoria, cualitativa y cuantitativa, con 37 estudiantes de una universidad pública del estado de Bahía, aprobada por el Comité de Ética. Se utilizó un cuestionario semiestructurado para la caracterización sociodemográfica y académica, un instrumento validado para la Evaluación de la Calidad de Vida: "The World Health Organization Quality of Life-BREF" (WHOQOL-bref), y dos preguntas abiertas. Percepción de los estudiantes sobre la relación entre experiencias académicas y calidad de vida. Los resultados del estudio revelaron que el perfil de la muestra fue mayoritariamente femenino, autodeclarado negro, con edades comprendidas entre los 20 y 25 años, en su mayoría del interior del estado, con ingresos complementarios por becas estudiantiles y estudiantes no de maestría. El dominio de calidad de vida más deteriorado fue físico y ambiental. La dimensión personal y de estudio de las experiencias académicas fueron las más afectadas. Es necesario dar voz a los estudiantes y desarrollar estrategias institucionales y políticas públicas para la formación universitaria y la permanencia, redundando en un mejor currículo de los universitarios.A Qualidade de Vida (QV) está relacionada ao conceito ampliado de saúde e bem-estar. A democratização do Ensino Superior possibilitou maior inserção de jovens nas universidades gerando necessidade de mais pesquisas sobre as repercussões acadêmicas na sua QV. Essa temática assume relevância quando tratamos das graduações em enfermagem, pois estes profissionais constituirão a maior força de trabalho da área da saúde.  Neste estudo objetivou-se compreender a percepção do estudante na relação entre vivências acadêmicas e qualidade de vida.  Foi uma pesquisa de campo, exploratória, quali-quantitativa, realizada com 37 estudantes universitários do curso de enfermagem de uma universidade pública do estado da Bahia, aprovada pelo Comitê de Ética em Pesquisa. Utilizou-se questionário semi-estruturado para traçar o perfil sociodemográfico e caracterização acadêmica, o instrumento validado para Avaliação de Qualidade de Vida: “The World Health Organization Quality of Life-BREF” (WHOQOL-bref), e duas questões abertas para a livre expressão da percepção dos estudantes na relação entre vivências acadêmicas e qualidade de vida. Os resultados do estudo revelaram que o perfil da amostra foi majoritariamente de pessoas do sexo feminino, autodeclaradas pretas, com idade entre 20 e 25 anos, de maioria procedente do interior do estado, com renda complementar de bolsas estudantis e dessemestralizados. O domínio de QV mais prejudicado foi o físico e o ambiental. A dimensão pessoal e de estudo das vivências acadêmicas foram as mais atingidas.  Torna-se necessário dar voz aos estudantes e desenvolver estratégias institucionais e políticas públicas para a formação e permanência universitária repercutindo na melhor QV dos estudantes universitários

Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection

ATLANTIC EPIPHYTES: a data set of vascular and non-vascular epiphyte plants and lichens from the Atlantic Forest

Epiphytes are hyper-diverse and one of the frequently undervalued life forms in plant surveys and biodiversity inventories. Epiphytes of the Atlantic Forest, one of the most endangered ecosystems in the world, have high endemism and radiated recently in the Pliocene. We aimed to (1) compile an extensive Atlantic Forest data set on vascular, non-vascular plants (including hemiepiphytes), and lichen epiphyte species occurrence and abundance; (2) describe the epiphyte distribution in the Atlantic Forest, in order to indicate future sampling efforts. Our work presents the first epiphyte data set with information on abundance and occurrence of epiphyte phorophyte species. All data compiled here come from three main sources provided by the authors: published sources (comprising peer-reviewed articles, books, and theses), unpublished data, and herbarium data. We compiled a data set composed of 2,095 species, from 89,270 holo/hemiepiphyte records, in the Atlantic Forest of Brazil, Argentina, Paraguay, and Uruguay, recorded from 1824 to early 2018. Most of the records were from qualitative data (occurrence only, 88%), well distributed throughout the Atlantic Forest. For quantitative records, the most common sampling method was individual trees (71%), followed by plot sampling (19%), and transect sampling (10%). Angiosperms (81%) were the most frequently registered group, and Bromeliaceae and Orchidaceae were the families with the greatest number of records (27,272 and 21,945, respectively). Ferns and Lycophytes presented fewer records than Angiosperms, and Polypodiaceae were the most recorded family, and more concentrated in the Southern and Southeastern regions. Data on non-vascular plants and lichens were scarce, with a few disjunct records concentrated in the Northeastern region of the Atlantic Forest. For all non-vascular plant records, Lejeuneaceae, a family of liverworts, was the most recorded family. We hope that our effort to organize scattered epiphyte data help advance the knowledge of epiphyte ecology, as well as our understanding of macroecological and biogeographical patterns in the Atlantic Forest. No copyright restrictions are associated with the data set. Please cite this Ecology Data Paper if the data are used in publication and teaching events. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ

Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis

CARVALHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-02-21T16:54:06Z No. of bitstreams: 1 Campos TM Matrix Metalloproteinase 9 Production....pdf: 913136 bytes, checksum: 65bd5278c2ab5bb0891996da8377ae88 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-02-21T17:09:59Z (GMT) No. of bitstreams: 1 Campos TM Matrix Metalloproteinase 9 Production....pdf: 913136 bytes, checksum: 65bd5278c2ab5bb0891996da8377ae88 (MD5)Made available in DSpace on 2018-02-21T17:09:59Z (GMT). No. of bitstreams: 1 Campos TM Matrix Metalloproteinase 9 Production....pdf: 913136 bytes, checksum: 65bd5278c2ab5bb0891996da8377ae88 (MD5) Previous issue date: 2014National Institutes of Health [grant AI088650] and Conselho Nacional de Pesquisa Instituto Nacional de Ciência e Tecnologia – Doenças Tropicais [grant 573839/2008-5].Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia. Doenças Tropicais. Salvador, BA, BrazilUniversity of Pennsylvania. Department of Pathobiology. Philadelphia, Pennsylvania, USAUniversity of Pennsylvania. Department of Pathobiology. Philadelphia, Pennsylvania, USAUniversidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, BrasilUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USAUniversity of Pennsylvania. Department of Pathobiology. Philadelphia, Pennsylvania, USAUniversidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia. Doenças Tropicais. Salvador, BA, BrazilUniversidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia. Doenças Tropicais. Salvador, BA, Brazil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilCutaneous leishmaniasis (CL) due to L.braziliensis infection is characterized by a strong inflammatory response with high levels of TNF and ulcer development. Less attention has been given to the role of mononuclear phagocytes to this process. Monocytes constitute a heterogeneous population subdivided into classical, intermediate and non-classical, and are known to migrate to inflammatory sites and secrete inflammatory mediators. TNF participates in the induction of matrix metalloproteinases (MMPs). MMP-9 is an enzyme that degrades basal membrane and its activity is controlled by the tissue inhibitor of metalloproteinase. Methods: Mononuclear cells were obtained from ex-vivo labeling sub-populations of monocytes and MMP-9, and the frequency was determined by flow cytometry. Culture was performed during 72 hours, stimulating the cells with SLA, levels of MMP-9 and TIMP-1 in the supernatants were determined by ELISA. Results: We observed that cells from CL lesions secrete high amounts of MMP-9 when compared to healthy subjects. Although MMP-9 was produced by monocytes, non-classical ones were the main source of this enzyme. We also observed that TNF produced in high level during CL contributes to MMP-9 production. Conclusions: These observations emphasize the role of monocytes, TNF and MMP-9 in the pathogenesis of L. braziliensis infection

Early Cutaneous Leishmaniasis Patients Infected With Leishmania braziliensis Express Increased Inflammatory Responses After Antimony Therapy

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-02-28T17:29:50Z No. of bitstreams: 1 Costa RS Early Cutaneous Leishmaniasis Patients Infected....pdf: 1578790 bytes, checksum: ebe3b7a6ad798d68d377b1aafe5d1de2 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-02-28T17:51:42Z (GMT) No. of bitstreams: 1 Costa RS Early Cutaneous Leishmaniasis Patients Infected....pdf: 1578790 bytes, checksum: ebe3b7a6ad798d68d377b1aafe5d1de2 (MD5)Made available in DSpace on 2018-02-28T17:51:42Z (GMT). No. of bitstreams: 1 Costa RS Early Cutaneous Leishmaniasis Patients Infected....pdf: 1578790 bytes, checksum: ebe3b7a6ad798d68d377b1aafe5d1de2 (MD5) Previous issue date: 2018National Institutes of Health (Grant U01 AI088650-06)Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / University of Pennsylvania. School of Veterinary Medicine. Department Pathobiology. Philadelphia, PAUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, BrasilUniversity of Pennsylvania. School of Veterinary Medicine. Department Pathobiology. Philadelphia, PAUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, BrasilEarly cutaneous leishmaniasis (ECL) is characterized by a nonulcerated papular lesion and illness duration less than 30 days. Approximately 4 weeks later, the cutaneous leishmaniasis (CL) ulcers appear. We were surprised to find that failure after antimony therapy (Sb5) is higher in ECL than CL. We hypothesize that the inflammatory response in ECL patients may increase during Sb5 therapy, which leads to treatment failure. Methods. A cohort of 44 ECL patients infected by Leishmania braziliensis was established to evaluate the response to Sb5 and to compare immunologic responses in ECL patients with CL and healthy subjects. Results. A hierarchical clustering based on cytokine levels showed a weak positive correlation between proinflammatory cytokine levels and those patients that failed Sb5 treatment. Although Sb5 therapy decreased interferon-γ and tumor necrosis factor levels in CL patients, we were surprised to find that an increase in these cytokines was observed in ECL patients. Moreover, interleukin (IL)-10 was less able to down-modulate immune responses in ECL. Conclusions. The enhanced production of proinflammatory cytokines, due in part to the decreased ability of IL-10 to down-modulate immune response during therapy in ECL, promotes the development and persistence of leishmania ulcer despite antimony therapy

Intermediate monocytes contribute to pathologic immune response in Leishmania braziliensis infections

Carvalho, Lucas Pedreira de; Carvalho Filho, Edgar Marcelino de. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-03-27T14:08:19Z No. of bitstreams: 1 Passos S Intermediate Monocytes Contribute to Pathologic....pdf: 464442 bytes, checksum: 809b229567ea215332f2068baab945bb (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-03-27T14:22:13Z (GMT) No. of bitstreams: 1 Passos S Intermediate Monocytes Contribute to Pathologic....pdf: 464442 bytes, checksum: 809b229567ea215332f2068baab945bb (MD5)Made available in DSpace on 2018-03-27T14:22:13Z (GMT). No. of bitstreams: 1 Passos S Intermediate Monocytes Contribute to Pathologic....pdf: 464442 bytes, checksum: 809b229567ea215332f2068baab945bb (MD5) Previous issue date: 2015National Institutes of Health (grant AI088650) and Conselho Nacional de Pesquisa Instituto Nacional de Ciência e Tecnologia–Doenças Tropicais (grant 573839/2008-5).Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia-Doenças Tropicais. Salvador, BA, Brasil.Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia-Doenças Tropicais. Salvador, BA, Brasil.Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, PA, USA.Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia-Doenças Tropicais. Salvador, BA, Brasil.Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia-Doenças Tropicais. Salvador, BA, Brasil.University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, PA, USA.University of Maryland. Department of Cell Biology and Molecular Genetics. College Park. Maryland.Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia-Doenças Tropicais. Salvador, BA, Brasil.University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, PA, USA.Ulcer development in patients with cutaneous leishmaniasis (CL) caused by Leishmania braziliensis is associated with high levels of tumor necrosis factor (TNF). We found that early after infection, before ulcer development, the frequency of CD16(+) (both intermediate [CD14(+)CD16(+)] and nonclassical [CD14(dim)CD16(+)]) monocytes was increased in the peripheral blood of patients with L. braziliensis, compared with uninfected controls. These results suggest that CD16(+) monocytes might promote disease. Also, we found that intermediate monocytes expressed CCR2 and that increased levels of CCL2 protein were present in lesions from patients, suggesting that intermediate monocytes are more likely than nonclassical monocytes to migrate to the lesion site. Finally, we found that the intermediate monocytes produced TNF. Our results show that intermediate monocytes are increased in frequency soon after infection; express CCR2, which would promote their migration into the lesions; and, owing to their production of TNF, can enhance the inflammatory response

Atherosclerotic Plaque in Patients with Zero Calcium Score at Coronary Computed Tomography Angiography

<div><p>Abstract Background: In view of the high mortality for cardiovascular diseases, it has become necessary to stratify the main risk factors and to choose the correct diagnostic modality. Studies have demonstrated that a zero calcium score (CS) is characteristic of a low risk for cardiovascular events. However, the prevalence of individuals with coronary atherosclerotic plaques and zero CS is conflicting in the specialized literature. Objective: To evaluate the frequency of patients with coronary atherosclerotic plaques, their degree of obstruction and associated factors in patients with zero CS and indication for coronary computed tomography angiography (CCTA). Methods: This is a cross-sectional, prospective study with 367 volunteers with zero CS at CCTA in four diagnostic imaging centers in the period from 2011 to 2016. A significance level of 5% and 95% confidence interval were adopted. Results: The frequency of atherosclerotic plaque in the coronary arteries in 367 patients with zero CS was 9.3% (34 individuals). In this subgroup, mean age was 52 ± 10 years, 18 (52.9%) were women and 16 (47%) had significant coronary obstructions (> 50%), with involvement of two or more segments in 4 (25%) patients. The frequency of non-obese individuals (90.6% vs 73.9%, p = 0.037) and alcohol drinkers (55.9% vs 34.8%, p = 0.015) was significantly higher in patients with atherosclerotic plaques, with an odds ratio of 3.4 for each of this variable. Conclusions: The frequency of atherosclerotic plaque with zero CS was relatively high, indicating that the absence of calcification does not exclude the presence of plaques, many of which obstructive, especially in non-obese subjects and alcohol drinkers.</p></div

Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil

During these past years, several studies have provided serological evidence regarding the circulation of West Nile virus (WNV) in Brazil. Despite some reports, much is still unknown regarding the genomic diversity and transmission dynamics of this virus in the country. Recently, genomic monitoring activities in horses revealed the circulation of WNV in several Brazilian regions. These findings on the paucity of genomic data reinforce the need for prompt investigation of WNV infection in horses, which may precede human cases of encephalitis in Brazil. Thus, in this study, we retrospectively screened 54 suspicious WNV samples collected between 2017 and 2020 from the spinal cord and brain of horses with encephalitis and generated three new WNV genomes from the Cear&aacute; and Bahia states, located in the northeastern region of Brazil. The Bayesian reconstruction revealed that at least two independent introduction events occurred in Brazil. The first introduction event appears to be likely related to the North American outbreak, and was estimated to have occurred in March 2013.The second introduction event appears to have occurred in September 2017 and appears to be likely related to the South American outbreak. Together, our results reinforce the importance of increasing the priority of WNV genomic monitoring in equines with encephalitis in order to track the dispersion of this emerging pathogen through the country