UTILIZAÇÃO DE ÓLEO DE GIRASSOL OZONIZADO NA ANTISSEPSIA E TRATAMENTO DE FERIDAS PÓS-CIRURGICAS EM GATAS.

Ozone is a gas found in the earth's crust, and its action on organic matter was discovered in 1840 by the German Friedrich Christian Schonbein. Ozone triggers several mechanisms that lead to the normalization of oxygen demand for several days, that is, it can favor more oxygenation, preventing ischemia and infections, favoring healing. Thus, the objective of this study was to demonstrate, in a qualitative way, the biological actions of ozonated sunflower oil in the pre and post-surgical treatment of cats submitted to routine ovarian saltingohysterectomy (OSH). The procedures were performed at the Veterinary Clinic BaraquetDieb, in Cidade Ocidental - GO, from August 12 to November 16, 2019. For this purpose, 10 cats were handled, separated into 02 groups of 05 animals each, being Group 01: Experimental (ozonated sunflower oil) and group 02: Control (Chlorhexidine 2%, Alcohol 70%, Saline 0.9% and Rifocin). Macroscopic results demonstrated that group 01 presented better tissue healing, however the healing time did not differ from group 02, making it possible to see it with the naked eye. It can be concluded with the results obtained, that the use of ozone therapy with the aid of sunflower oil was effective in the healing process of surgical wounds in cats.O Ozônio é um gás encontrado na crosta terrestre, e foi descoberta sua ação na matéria orgânica no ano de 1840 pelo alemão Friedrich Christian Schonbein. O ozônio desencadeia vários mecanismos que levam à normalização da demanda de oxigênio por vários dias, ou seja, pode favorecer mais oxigenação impedindo isquemia e infecções, favorecendo a cicatrização. Sendo assim, o objetivo desse estudo foi demonstrar, de forma qualitativa, as ações biológicas do óleo de girassol ozonizado no tratamento pré e pós-cirúrgico de gatas submetidas a ováriosalpingohisterectomia (OSH) de rotina. Os procedimentos foram realizados na Clínica Veterinária BaraquetDieb, em Cidade Ocidental – GO no período de 12 de agosto a 16 de novembro do ano de 2019. Para tal, foram manipuladas 10 gatas, separadas em 02 grupos de 05 animais cada, sendo o Grupo 01: Experimental (Óleo de girassol ozonizado) e o grupo 02: Controle (Clorexidina 2%, Álcool 70%, Solução Fisiológica 0,9% e Rifocina). Resultados macroscópicos demonstraram que o grupo 01 apresentou melhor cicatrização tecidual, porém o tempo de cicatrização não se diferiu do grupo 02 sendo possível visualização a olho nu. Pode-se concluir com os resultados obtidos, que o uso da ozonioterapia com o auxílio do óleo de girassol foi eficaz no processo de cicatrização de feridas cirúrgicas em gatas

Risk Factors for Different Types of Traumatic Injuries in Primary Teeth

Objective: To evaluate traumatic dental injuries (TDI) in primary teeth and the association of gender and age with different injuries. Material and Methods: Records of patients with TDI in primary teeth were included. The following parameters were registered: gender and age, place of trauma, cause of trauma, affected tissue and tooth, number of injured teeth, type of injury, and gingival and bone damage. A logistic regression analysis was performed to explore the interaction between gender and age on the occurrence of types of injuries (p≤0.05). Results: The total of 721 records were evaluated and 370 records were included, being 61.6% boys and 60.5% children aged 0–3 years old, with 658 primary teeth affected. The support tissue was most affected (496/658), followed by dental tissue (139/658). Enamel/dentine fracture with pulp exposure (n=51) and intrusion (n=131) were the most common injuries of dental and support tissues, respectively. In general, boys suffered more traumas than girls, regardless of the age range. As for concussion, logistic regression confirms that gender and age are also influencers. Girls (OR=1.822, CI = 1.050-3.164, p=0.033) in the 4–6 year age group (OR=2.15, CI = 1.239–3.747, p=0.007) are more likely to have concussions. Children age 4–6 years were less likely to suffer an intrusion (OR=0.496; CI = 0.278– 0.886; p=0.018). Conclusion: Gender and age influence concussion and intrusion in the primary teeth

Use of statistical multivariate analysis to identify sources emitting particulate matter in Belo Horizonte, Brazil

Particulate matter is the mixture of solid and liquid particles. It may at times cause adverse effects to human health, in the climate, ecosystem and materials. The objective of this study was to identify the sources emitting particulate matter that contributes to environmental pollution at two sites in Belo Horizonte city. The metropolitan region of Belo Horizonte is characterized by numerous mining activities and other industries, high population and, consequently, a high number of vehicles. There are therefore several sources emitting pollutants into the atmosphere. Airborne particulate matter was collected in air filters during approximately one year using a high-volume sampler in strategic points. The concentration of particulate matter results showed that, in general, the values were 40% higher than the values recommended by legislation. During the summer rainy season, no sample exceeded the recommended limits at both sampling sites. The elemental composition was determined through neutron activation analysis and these values were considered variables in the multivariate statistical analysis for the identification of the main sources of particle emission and their contributions. The results confirmed a significant influence of the soil and motor vehicles in the degradation of the region’s air quality

Evidence of bovine viral diarrhea virus transmission by back pond water in experimentally infected piglets

ABSTRACT: Swine can be infected by bovine viral diarrhea virus (BVDV). However, transmission routes among pigs are still unknown. The objective of the present study was to induce experimental infection of BVDV-1 in weaned piglets and to assess the potential transmission through pen back pond water, used to facilitate heat exchange of the pigs housed in barns. Two repetitions (BP1 and BP 2) were performed using 12 piglets proven to be free BVDV (n=6 per repetition) allocated into three groups: control, sentinels and infected with two piglets each. The piglets were placed in stainless steel isolators. The infected group received an inoculum containing BVDV-1, Singer strain. The piglets remained in the cabinets for 25 days, during which samples of nasal swab were collected daily and blood sampled weekly. At the end, the piglets were euthanized, necropsied and organ fragments were collected for histopathology, immunohistochemistry and RT-PCR. In the first experiment (BP1) the infected animals shed the virus between days 6 and 21 post-infection. Regarding the sentinel group, shedding occurred in only one piglet, on the 20th day after infection, and seroconversion was observed on the 25th day post-infection. In BP2, infected piglets I3 and I4 shed the virus on days 4 and 21 post-infection, respectively. Only one sentinel piglet (S3) she the virus on day 13 post-infection. Therefore, it was concluded that pigs can become infected with BVDV-1 and shed potentially infectious viral particles consequently, being able to transmit the virus to other pigs through back pond water

Gut microbiota modulation induced by Zika virus infection in immunocompetent mice

Abstract Gut microbiota composition can modulate neuroendocrine function, inflammation, and cellular and immunological responses against different pathogens, including viruses. Zika virus (ZIKV) can infect adult immunocompetent individuals and trigger brain damage and antiviral responses. However, it is not known whether ZIKV infection could impact the gut microbiome from adult immunocompetent mice. Here, we investigated modifications induced by ZIKV infection in the gut microbiome of immunocompetent C57BL/6J mice. Adult C57BL/6J mice were infected with ZIKV and the gut microbiota composition was analyzed by next-generation sequencing of the V4 hypervariable region present in the bacterial 16S rDNA gene. Our data showed that ZIKV infection triggered a significant decrease in the bacteria belonging to Actinobacteria and Firmicutes phyla, and increased Deferribacteres and Spirochaetes phyla components compared to uninfected mice. Interestingly, ZIKV infection triggered a significant increase in the abundance of bacteria from the Spirochaetaceae family in the gut microbiota. Lastly, we demonstrated that modulation of microbiota induced by ZIKV infection may lead to intestinal epithelium damage and intense leukocyte recruitment to the intestinal mucosa. Taken together, our data demonstrate that ZIKV infection can impact the gut microbiota composition and colon tissue homeostasis in adult immunocompetent mice