The CARIMED (CARbon IN the MEDiterranean Sea) data synthesis initiative: overview and quality control procedures

posterIn this work we present the data synthesis project CARIMED (CARbon in the MEDiterranean Sea), we aim to create a uniformly formatted consistent quality controlled public database for carbon relevant variables from hydrographic cruises covering the whole water column and the different basins in the MedSea. Both primary and secondary quality control (QC) of the data has been performed following the experience gathered in CARINA and GLODAPv2. The motivation for this initiative stemmed from two CIESM (Mediterranean Science Commission) workshops, the first one in Menton (France) October 2008 (CIESM, 2008) focused on the impact of OA on biological, chemical and physical systems in the MedSea, and the second one in Supetar (Croatia) May 2011 (CIESM, 2012) focused on designing the Mediterranean Sea repeat hydrography program (MED-SHIP). The unresolved issues regarding the CO2 system in the MedSea were summarized in Malanotte-Rizzoli et al. (Oc Sc, 2014), one of them the temporal and spatial variability of the interior CO2 system, clearly justifies the need for CARIMED. Independently two projects focused on compiling CO2 water column data in the MedSea, an initiative within the EU MedSeA project (Gemayel et al., ESD 2015) called MEDICA (T. Lovato personal communication) and the one here presented lead by the Spanish IEO and partially funded by SanLeón-Bartolomé 's PhD project. We hope a product like this will be much welcome by the oceanographic community, both observationist and modellers, as it was the release of the Meteor cruise M84/3 data in CDIAC used in several publications (Palmieri et al., BG 2015; Cossarini et al., BG 2015; Hassoun et al., DSR 2015 & JWROS 2015; Lovato & Vichi, DSR 2015; Gemayel et al., ESD 2015)

The CO2 system in the Mediterranean Sea: a basin wide perspective.

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Interaction between elevated co2 and organic matter on bacterial metabolismo

Aquatic Sciences Meeting (Aquatic Sciences: Global And Regional Perspectives - North Meets South), 22-27 February 2015, Granada, Spain.Microcosm experiments to assess bacterioplankton response to autochthonous inputs of organic matter modified by future acidified ocean conditions were performed. Surface seawater enriched with inorganic nutrients and incubated in UVR-transparent cubitainers was bubbled for 8 days with regular air (380 ppmv CO2) or with a high CO2-air mixture (1000 ppmv CO2) to be used as inocula. In the second phase of the experiment, natural bacterioplankton communities enriched with the acidified or non-acidified organic matter inocula were incubated under dark conditions during 8 days in the presence or absence of CO2 as previously. Bacterial abundance, production and viability were measured as physiological indicators of bacterial metabolism. The results showed that acidified organic matter produced higher abundances for similar production rates early during the incubation, while non-acidified organic matter produced higher bacterial production and viability latter at the end of the experiment, indicating a more recalcitrant character of the organic matter under these conditions. We demonstrate that CO2 effects on bacterioplankton are mainly due to indirect effects on organic matter characteristics rather than to direct effects of acidification on bacteria metabolism.N

Effects of elevated CO₂ and phytoplankton-derived organic matter on the metabolism of bacterial communities from coastal waters

Microcosm experiments to assess the bacterioplankton's response to phytoplankton-derived organic matter obtained under current and future ocean CO2 levels were performed. Surface seawater enriched with inorganic nutrients was bubbled for 8 days with air (current CO2 scenario) or with a 1000&thinsp;ppm CO2 air mixture (future CO2 scenario) under solar radiation. The organic matter produced under the current and future CO2 scenarios was subsequently used as an inoculum. Triplicate 12&thinsp;L flasks filled with 1.2&thinsp;µm of filtered natural seawater enriched with the organic matter inocula were incubated in the dark for 8 days under CO2 conditions simulating current and future CO2 scenarios, to study the bacterial response. The acidification of the media increased bacterial respiration at the beginning of the experiment, while the addition of the organic matter produced under future levels of CO2 was related to changes in bacterial production and abundance. This resulted in a 67&thinsp;% increase in the integrated bacterial respiration under future CO2 conditions compared to present CO2 conditions and 41&thinsp;% higher integrated bacterial abundance with the addition of the acidified organic matter compared to samples with the addition of non acidified organic matter. This study demonstrates that the increase in atmospheric CO2 levels can impact bacterioplankton metabolism directly, by changes in the respiration rate, and indirectly, by changes on the organic matter, which affected bacterial production and abundance.</p