43 research outputs found
Como escrevemos sobre a educação que está sendo? Linhas refletivas para uma investigação da praxe educacional e política dos movimentos sociais populares autónomos
The material that is offered here responds to a deep motivation that has the intention of feel-thinking from other sources the question of pedagogies at this time, which stress the logics of neoliberal capitalism and its ways of become a body in the territories. Assuming the complexity of an approach that does not reproduce the colonial ways of doing Social Sciences, these pages are limited to raising some questions and provisional statements about education as a constitutive dimension of the praxis of popular social autonomous movements, conducting a documentary analysis of theoretical methodological lines. Among the unavoidable references for reflection, there is the work of Elisa Cragnolino on the Peasant Movement of Córdoba (MCC) and the pedagogical projects developed in recent years; also the contributions of Oscar Soto, Lia Pinheiro Barbosa, Miguel Arroyo, Norma Michi, Diana Vila and Álvaro Di Matteo, Mercedes Palumbo and Anahí Guelman. In this diversity of approaches and experiences I am interested in questioning what the different intellectuals highlight about the characterizations offered of these pedagogies deployed in praxis, the criticisms of the school of the official system and the particularities of the processes of subjectivation that they display.El material que aquí se ofrece, responde a una motivación profunda que tiene como voluntad sentipensar desde otras matrices la cuestión de las pedagogías que, en este tiempo, tensionan las lógicas del capitalismo neoliberal y sus formas de hacerse cuerpo en los territorios. Asumiendo la complejidad de un abordaje que no reproduzca las formas coloniales de hacer Ciencias Sociales, estas páginas se circunscriben a relevar algunos interrogantes y afirmaciones provisorias sobre la educación como dimensión constitutiva de la praxis de los movimientos sociales populares autónomos, realizando un análisis documental de líneas teórico metodológicas. Entre las referencias ineludibles, se encuentra el trabajo de Elisa Cragnolino sobre el Movimiento Campesino de Córdoba (MCC) y los proyectos pedagógicos desarrollados en los últimos años; también los aportes de Oscar Soto, Lia Pinheiro Barbosa, Miguel Arroyo, Norma Michi, Diana Vila y Álvaro Di Matteo, Mercedes Palumbo y Anahí Guelman. En esta diversidad de planteos y experiencias me interesa interrogar e interpretar lo que lxs distintxs intelectuales destacan en torno a las caracterizaciones que se ofrecen de estas pedagogías desplegadas en la praxis, las críticas a la escuela del sistema oficial y las particularidades que revisten los procesos de subjetivación que despliegan.O material que aqui se oferece responde a uma motivação profunda que pretende senti-pensar a partir de outras matrizes a questão das pedagogias que, neste momento, sublinham a lógica do capitalismo neoliberal e os seus modos de se constituir corpo nos territórios. Partindo do pressuposto da complexidade de uma abordagem que não reproduz os modos coloniais de fazer Ciências Sociais, estas páginas limitam-se a levantar algumas questões e afirmações provisórias sobre a educação como dimensão constitutiva da práxis dos movimentos sociais populares autônomos, realizando uma analise documental de linhas teoricas metodologicas. Entre as referências incontornáveis de reflexão, encontram-se a atuação de Elisa Cragnolino sobre o Movimento Camponês de Córdoba (MCC) e os projetos pedagógicos desenvolvidos nos últimos anos; Também as contribuições de Oscar Soto, Lia Pinheiro Barbosa, Miguel Arroyo, Norma Michi, Diana Vila e Álvaro Di Matteo, Mercedes Palumbo e Anahí Guelman. Nessa diversidade de abordagens e experiências, interessa-me questionar o que os diferentes intelectuais destacam sobre as caracterizações oferecidas dessas pedagogias implantadas na práxis, as críticas da escola ao sistema oficial e as particularidades que os processos de educação possuem
Fotoperiodismo en la Guerra Civil española: La mirada de Agustí Centelles a través de su legado en la Universidad de Zaragoza.
A lo largo de la Historia, los conflictos bélicos y sociales han sido el máximo exponente del ejercicio del fotoperiodismo, que comienza a desarrollarse en la segunda mitad del siglo XIX. La Guerra Civil española supone un hito fundamental en este oficio puesto que, además de darse en un momento de adelantos técnicos en la fotografía y de auge en las revistas gráficas, reúne a fotógrafos nacionales y extranjeros, activistas sobre todo en el bando republicano. Entre ellos, el fotógrafo Agustí Centelles fue uno de los principales reporteros gráficos que tomó partida en la guerra con una mirada moderna y militante, sobreviviendo al exilio y salvando con él su archivo fotográfico que, a día de hoy, constituye un legado valiosísimo para nuestra memoria histórica. En este trabajo se analiza su obra a través del archivo perteneciente al patrimonio de la Universidad de Zaragoza
Cd38 deficiency ameliorates chronic graft versus Host disease murine lupus via a b-cell dependent mechanism
Trabajo presentado en el II Congreso investigación PTS, celebrado en Granada (España) del 09 al 11 de febrero de 2022.Absence of mouse cell surface receptor CD38 in Cd38-/- mice suggests that this receptor acts as positive regulator of inflammatory and autoimmune responses. Here we report that in the setting of a chronic graft versus host disease (cGVHD) lupus model induced by the transfer of B6.C-H2bm12/KhEg (bm12) spleen cells into co-isogenic Cd38-/- B6 mice causes milder lupus-like autoimmunity with lower levels of anti-ssDNA autoantibodies than the transfer of bm12 spleen cells into WT B6 mice. I In addition, significantly lower percentages of Tfh cells, as well as GC B cells, plasma cells and T-bet+CD11chi B cells are observed in Cd38-/- mice than in WT mice, while the expansion of Treg cells, and Tfr cells is normal, suggesting that the ability of Cd38-/- B cells to respond to allogeneic help from bm12 CD4+ T cells is greatly diminished. The frequencies of T-bet+CD11chi B cells, which are considered the precursors of the autoantibody secreting cells, correlate with anti-ssDNA autoantibody serum levels, with IL-27, and sCD40L. Proteomics profiling of spleens from WT cGVHD mice reflects a STAT1-driven type I IFN-signature, which is absent in Cd38-/- cGVHD mice. Kidney, spleen and liver inflammation was mild and resolved faster in Cd38-/- cGVHD mice than in WT cGVHD mice. We conclude that in B cells CD38 functions as a modulator receptor that controls autoimmune responses
CD38 Deficiency Ameliorates Chronic Graft-Versus-Host Disease Murine Lupus via a B-Cell-Dependent Mechanism
© 2021 Martínez-Blanco, Domínguez-Pantoja, Botía-Sánchez, Pérez-Cabrera, Bello-Iglesias, Carrillo-Rodríguez, Martin-Morales, Lario-Simón, Pérez-Sánchez-Cañete, Montosa-Hidalgo, Guerrero-Fernández, Longobardo-Polanco, Redondo-Sánchez, Cornet-Gomez, Torres-Sáez, Fernández-Ibáñez, Terrón-Camero, Andrés-León, O’Valle, Merino, Zubiaur and Sancho.The absence of the mouse cell surface receptor CD38 in Cd38−/− mice suggests that this receptor acts as a positive regulator of inflammatory and autoimmune responses. Here, we report that, in the context of the chronic graft-versus-host disease (cGVHD) lupus inducible model, the transfer of B6.C-H2bm12/KhEg(bm12) spleen cells into co-isogenic Cd38−/− B6 mice causes milder lupus-like autoimmunity with lower levels of anti-ssDNA autoantibodies than the transfer of bm12 spleen cells into WT B6 mice. In addition, significantly lower percentages of Tfh cells, as well as GC B cells, plasma cells, and T-bet+CD11chi B cells, were observed in Cd38−/− mice than in WT mice, while the expansion of Treg cells and Tfr cells was normal, suggesting that the ability of Cd38−/− B cells to respond to allogeneic help from bm12 CD4+ T cells is greatly diminished. The frequencies of T-bet+CD11chi B cells, which are considered the precursors of the autoantibody-secreting cells, correlate with anti-ssDNA autoantibody serum levels, IL-27, and sCD40L. Proteomics profiling of the spleens from WT cGVHD mice reflects a STAT1-driven type I IFN signature, which is absent in Cd38−/− cGVHD mice. Kidney, spleen, and liver inflammation was mild and resolved faster in Cd38−/− cGVHD mice than in WT cGVHD mice. We conclude that CD38 in B cells functions as a modulator receptor that controls autoimmune responses.S and MZ received financial support through “Proyecto del Plan Estatal”: SAF2017–89801-R. The IPBLN-CSIC Proteomics Unit belonged to ProteoRed-ISCIII (PRB2; PRB3) and was supported by grants PT13/0001/0011 (IPBLN-CSIC) and PT17/0019/0010 (CIB-CSIC; IPBLN-CSIC). RM: Project: SAF2017-82905-R. FO'V: Cátedra MIS IMPLANT-UGR. The stay of AC-G in Sancho’s lab was supported by a fellowship-contract JAE-Intro (CSIC). The stay of MD-P in Sancho’s lab was supported by a 1-year post-doctoral fellowship (Reference No. 502492) from the Consejo Nacional de Ciencia y Tecnología (CONACYT) of México. EA-L was recipient of a postdoctoral fellowship from the regional Andalusian Government
Extracellular vesicles from pristane-treated CD38-deficient mice express an antiinflammatory neutrophil protein signature, which reflects the mild lupus severity elicited in these mice
In CD38-deficient (Cd38-/-) mice intraperitoneal injection of pristane induces a
lupus-like disease, which is milder than that induced in WT mice, showing
significant differences in the inflammatory and autoimmune processes
triggered by pristane. Extracellular vesicles (EV) are present in all body fluids.
Shed by cells, their molecular make-up reflects that of their cell of origin and/or
tissue pathological situation. The aim of this study was to analyze the protein
composition, protein abundance, and functional clustering of EV released by
peritoneal exudate cells (PECs) in the pristane experimental lupus model, to
identify predictive or diagnostic biomarkers that might discriminate the
autoimmune process in lupus from inflammatory reactions and/or normal
physiological processes. In this study, thanks to an extensive proteomic
analysis and powerful bioinformatics software, distinct EV subtypes were identified in the peritoneal exudates of pristane-treated mice: 1) small EV
enriched in the tetraspanin CD63 and CD9, which are likely of exosomal
origin; 2) small EV enriched in CD47 and CD9, which are also enriched in
plasma-membrane, membrane-associated proteins, with an ectosomal origin;
3) small EV enriched in keratins, ECM proteins, complement/coagulation
proteins, fibrin clot formation proteins, and endopetidase inhibitor proteins.
This enrichment may have an inflammation-mediated mesothelial-tomesenchymal
transition origin, representing a protein corona on the surface
of peritoneal exudate EV; 4) HDL-enriched lipoprotein particles. Quantitative
proteomic analysis allowed us to identify an anti-inflammatory, Annexin A1-
enriched pro-resolving, neutrophil protein signature, which was more
prominent in EV from pristane-treated Cd38-/- mice, and quantitative
differences in the protein cargo of the ECM-enriched EV from Cd38-/- vs WT
mice. These differences are likely to be related with the distinct inflammatory
outcome shown by Cd38-/- vs WT mice in response to pristane treatment. Our
results demonstrate the power of a hypothesis-free and data-driven approach
to transform the heterogeneity of the peritoneal exudate EV from pristanetreated
mice in valuable information about the relative proportion of different
EV in a given sample and to identify potential protein markers specific for the
different small EV subtypes, in particular those proteins defining EV involved in
the resolution phase of chronic inflammation.Proyecto del plan estatal, Ministerio de Ciencia e Innovacion PT13/0001/011CSIC PT17/0019/0010
PID2020-119567RB-I0
Standardization in laboratory medicine: Two years’ experience from category 1 EQA programs in Spain
Introduction: Standardization is the ability to obtain interchangeable results leading to same medical interpretation. External quality assessment
(EQA) is the main support of the on-going harmonization initiatives. Aim of study was to evaluate results obtained from two years category 1 EQA
program experience in Spain and determine the impact of applying this type of EQA program on the analytical standardization.
Materials and methods: According to the analytical method, traceability and instrument different groups were established which results were
evaluated by calculating mean, coefficient of variation and percent of deviation to the reference value. Analytical performance specifications used
to the results\u27 evaluation were derived from biological variation for bias and from the inter-laboratory coefficients of variation found in a previous
pilot study.
Results: Only creatinine measured by enzymatic methods gave excellent results, although few laboratories used this method. Creatine kinase and
GGT gave good precision and bias in all, but one instrument studied. For the remaining analytes (ALT, ALP, AST, bilirubin, calcium, chloride, glucose,
magnesium, potassium, sodium, total protein and urate) some improvement is still necessary to achieve satisfactory standardization in our setting.
Conclusions: The two years of category 1 EQA program experience in Spain have manifested a lack of standardization of 17 most frequent biochemistry
tests used in our laboratories. The impact of the information obtained on the lack of standardization is to recommend abandoning methods
such as ALT, AST without exogenous pyridoxal phosphate, Jaffe method for creatinine, and do not use non-commutable calibrators, such as aqueous
solutions for calcium and sodium
PDRs4All IV. An embarrassment of riches: Aromatic infrared bands in the Orion Bar
(Abridged) Mid-infrared observations of photodissociation regions (PDRs) are
dominated by strong emission features called aromatic infrared bands (AIBs).
The most prominent AIBs are found at 3.3, 6.2, 7.7, 8.6, and 11.2 m. The
most sensitive, highest-resolution infrared spectral imaging data ever taken of
the prototypical PDR, the Orion Bar, have been captured by JWST. We provide an
inventory of the AIBs found in the Orion Bar, along with mid-IR template
spectra from five distinct regions in the Bar: the molecular PDR, the atomic
PDR, and the HII region. We use JWST NIRSpec IFU and MIRI MRS observations of
the Orion Bar from the JWST Early Release Science Program, PDRs4All (ID: 1288).
We extract five template spectra to represent the morphology and environment of
the Orion Bar PDR. The superb sensitivity and the spectral and spatial
resolution of these JWST observations reveal many details of the AIB emission
and enable an improved characterization of their detailed profile shapes and
sub-components. While the spectra are dominated by the well-known AIBs at 3.3,
6.2, 7.7, 8.6, 11.2, and 12.7 m, a wealth of weaker features and
sub-components are present. We report trends in the widths and relative
strengths of AIBs across the five template spectra. These trends yield valuable
insight into the photochemical evolution of PAHs, such as the evolution
responsible for the shift of 11.2 m AIB emission from class B in
the molecular PDR to class A in the PDR surface layers. This
photochemical evolution is driven by the increased importance of FUV processing
in the PDR surface layers, resulting in a "weeding out" of the weakest links of
the PAH family in these layers. For now, these JWST observations are consistent
with a model in which the underlying PAH family is composed of a few species:
the so-called 'grandPAHs'.Comment: 25 pages, 10 figures, to appear in A&
PDRs4All II: JWST's NIR and MIR imaging view of the Orion Nebula
The JWST has captured the most detailed and sharpest infrared images ever
taken of the inner region of the Orion Nebula, the nearest massive star
formation region, and a prototypical highly irradiated dense photo-dissociation
region (PDR). We investigate the fundamental interaction of far-ultraviolet
photons with molecular clouds. The transitions across the ionization front
(IF), dissociation front (DF), and the molecular cloud are studied at
high-angular resolution. These transitions are relevant to understanding the
effects of radiative feedback from massive stars and the dominant physical and
chemical processes that lead to the IR emission that JWST will detect in many
Galactic and extragalactic environments. Due to the proximity of the Orion
Nebula and the unprecedented angular resolution of JWST, these data reveal that
the molecular cloud borders are hyper structured at small angular scales of
0.1-1" (0.0002-0.002 pc or 40-400 au at 414 pc). A diverse set of features are
observed such as ridges, waves, globules and photoevaporated protoplanetary
disks. At the PDR atomic to molecular transition, several bright features are
detected that are associated with the highly irradiated surroundings of the
dense molecular condensations and embedded young star. Toward the Orion Bar
PDR, a highly sculpted interface is detected with sharp edges and density
increases near the IF and DF. This was predicted by previous modeling studies,
but the fronts were unresolved in most tracers. A complex, structured, and
folded DF surface was traced by the H2 lines. This dataset was used to revisit
the commonly adopted 2D PDR structure of the Orion Bar. JWST provides us with a
complete view of the PDR, all the way from the PDR edge to the substructured
dense region, and this allowed us to determine, in detail, where the emission
of the atomic and molecular lines, aromatic bands, and dust originate
PDRs4All III: JWST's NIR spectroscopic view of the Orion Bar
(Abridged) We investigate the impact of radiative feedback from massive stars
on their natal cloud and focus on the transition from the HII region to the
atomic PDR (crossing the ionisation front (IF)), and the subsequent transition
to the molecular PDR (crossing the dissociation front (DF)). We use
high-resolution near-IR integral field spectroscopic data from NIRSpec on JWST
to observe the Orion Bar PDR as part of the PDRs4All JWST Early Release Science
Program. The NIRSpec data reveal a forest of lines including, but not limited
to, HeI, HI, and CI recombination lines, ionic lines, OI and NI fluorescence
lines, Aromatic Infrared Bands (AIBs including aromatic CH, aliphatic CH, and
their CD counterparts), CO2 ice, pure rotational and ro-vibrational lines from
H2, and ro-vibrational lines HD, CO, and CH+, most of them detected for the
first time towards a PDR. Their spatial distribution resolves the H and He
ionisation structure in the Huygens region, gives insight into the geometry of
the Bar, and confirms the large-scale stratification of PDRs. We observe
numerous smaller scale structures whose typical size decreases with distance
from Ori C and IR lines from CI, if solely arising from radiative recombination
and cascade, reveal very high gas temperatures consistent with the hot
irradiated surface of small-scale dense clumps deep inside the PDR. The H2
lines reveal multiple, prominent filaments which exhibit different
characteristics. This leaves the impression of a "terraced" transition from the
predominantly atomic surface region to the CO-rich molecular zone deeper in.
This study showcases the discovery space created by JWST to further our
understanding of the impact radiation from young stars has on their natal
molecular cloud and proto-planetary disk, which touches on star- and planet
formation as well as galaxy evolution.Comment: 52 pages, 30 figures, submitted to A&