178 research outputs found
In-flight thrust determination on a real-time basis
A real time computer program was implemented on a F-15 jet fighter to monitor in-flight engine performance of a Digital Electronic Engine Controlled (DEES) F-100 engine. The application of two gas generator methods to calculate in-flight thrust real time is described. A comparison was made between the actual results and those predicted by an engine model simulation. The percent difference between the two methods was compared to the predicted uncertainty based on instrumentation and model uncertainty and agreed closely with the results found during altitude facility testing. Data was obtained from acceleration runs of various altitudes at maximum power settings with and without afterburner. Real time in-flight thrust measurement was a major advancement to flight test productivity and was accomplished with no loss in accuracy over previous post flight methods
Flow direction measurement criteria and techniques planned for the 40- by 80-/80- x 120-foot wind tunnel integrated systems tests
A study was performed in order to develop the criteria for the selection of flow direction indicators for use in the Integrated Systems Tests (ISTs) of the 40 by 80/80 by 120 Foot Wind Tunnel System. The problems, requirements, and limitations of flow direction measurement in the wind tunnel were investigated. The locations and types of flow direction measurements planned in the facility were discussed. A review of current methods of flow direction measurement was made and the most suitable technique for each location was chosen. A flow direction vane for each location was chosen. A flow direction vane that employs a Hall Effect Transducer was then developed and evaluated for application during the ISTs
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Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule.
Progressive organ fibrosis accounts for one-third of all deaths worldwide, yet preclinical models that mimic the complex, progressive nature of the disease are lacking, and hence, there are no curative therapies. Progressive fibrosis across organs shares common cellular and molecular pathways involving chronic injury, inflammation, and aberrant repair resulting in deposition of extracellular matrix, organ remodeling, and ultimately organ failure. We describe the generation and characterization of an in vitro progressive fibrosis model that uses cell types derived from induced pluripotent stem cells. Our model produces endogenous activated transforming growth factor β (TGF-β) and contains activated fibroblastic aggregates that progressively increase in size and stiffness with activation of known fibrotic molecular and cellular changes. We used this model as a phenotypic drug discovery platform for modulators of fibrosis. We validated this platform by identifying a compound that promotes resolution of fibrosis in in vivo and ex vivo models of ocular and lung fibrosis
Filmmaking education and enterprise culture: an ethnographic exploration of two filmmaking education contexts and their relation to bedroom culture and the creative workplace
Filmmaking education has never been firmly integrated into schooling and in past years has suffered from cuts to funding for youth work and formal and non-formal arts education. It continues to exist only by drawing on creative industry and cultural consumption practices as well as state funding. In this paper we explore the filmmaking education contexts we encountered while doing our own pieces of year-long ethnographic research. These contexts import 'enterprising' ways of thinking, doing and being from the creative workplace and 'bedroom culture'. Located across life's domains, they address enterprising subjects who take pleasure in work, make use of leisure, and who are always learning. We argue that these filmmaking education contexts support young people to develop their private creative practice and introduce them to the possibility of work in the creative industries but, because of the enterprise culture in which they are entangled, uncritically address these young people as enterprising subjects
Detection of magnetic dipole lines of Fe XII in the ultraviolet spectrum of the dwarf star Epsilon Eri
We report observations of the dwarf star Epsilon Eri (K2 V) made with the
Space Telescope Imaging Spectrograph (STIS) on the Hubble Space Telescope
(HST). The high sensitivity of the STIS instrument has allowed us to detect the
magnetic dipole transitions of Fe XII at 1242.00A and 1349.38A for the first
time in a star other than the Sun. The width of the stronger line at 1242.00A
has also been measured; such measurements are not possible for the permitted
lines of Fe XII in the extreme ultraviolet. To within the accurcy of the
measurements, the N V and the Fe XII lines occur at their rest wavelengths.
Electron densities and line widths have been measured from other transition
region lines. Together, these can be used to investigate the non-thermal energy
flux in the lower and upper transition region, which is useful in constraining
possible heating processes. The Fe XII lines are also present in archival STIS
spectra of other G/K-type dwarfs.Comment: 12 pages, 4 figures, submitted to MNRAS letters (11 Jan 2001
Neutral lipids associated with haemozoin mediate efficient and rapid β-haematin formation at physiological pH, temperature and ionic composition
Spectroscopic Coronal Observations during the Total Solar Eclipse of 11 July 2010
The flash spectrum of the solar chromosphere and corona was measured with a
slitless spectrograph before, after, and during the totality of the solar
eclipse, of 11 July 2010, at Easter Island, Chile. This eclipse took place at
the beginning of the Solar Cycle 24, after an extended minimum of solar
activity. The spectra taken during the eclipse show a different intensity ratio
of the red and green coronal lines compared with those taken during the total
solar eclipse of 1 August 2008, which took place towards the end of the Solar
Cycle 23. The characteristic coronal forbidden emission line of forbidden Fe
XIV (5303 {\AA}) was observed on the east and west solar limbs in four areas
relatively symmetrically located with respect to the solar rotation axis.
Subtraction of the continuum flash-spectrum background led to the
identification of several extremely weak emission lines, including forbidden Ca
XV (5694 {\AA}), which is normally detected only in regions of very high
excitation, e.g., during flares or above large sunspots. The height of the
chromosphere was measured spectrophotometrically, using spectral lines from
light elements and compared with the equivalent height of the lower
chromosphere measured using spectral lines from heavy elements.Comment: 14 pages, 8 figures, 1 table; Solar Physics, 2012, Februar
High Yield Production Process for Shigella Outer Membrane Particles
Gram-negative bacteria naturally shed particles that consist of outer membrane lipids, outer membrane proteins, and soluble periplasmic components. These particles have been proposed for use as vaccines but the yield has been problematic. We developed a high yielding production process of genetically derived outer membrane particles from the human pathogen Shigella sonnei. Yields of approximately 100 milligrams of membrane-associated proteins per liter of fermentation were obtained from cultures of S. sonnei ΔtolR ΔgalU at optical densities of 30–45 in a 5 L fermenter. Proteomic analysis of the purified particles showed the preparation to primarily contain predicted outer membrane and periplasmic proteins. These were highly immunogenic in mice. The production of these outer membrane particles from high density cultivation of bacteria supports the feasibility of scaling up this approach as an affordable manufacturing process. Furthermore, we demonstrate the feasibility of using this process with other genetic manipulations e.g. abolition of O antigen synthesis and modification of the lipopolysaccharide structure in order to modify the immunogenicity or reactogenicity of the particles. This work provides the basis for a large scale manufacturing process of Generalized Modules of Membrane Antigens (GMMA) for production of vaccines from Gram-negative bacteria
LPS unmasking of Shigella flexneri reveals preferential localisation of tagged outer membrane protease IcsP to septa and new poles
The Shigella flexneri outer membrane (OM) protease IcsP (SopA) is a member of the enterobacterial Omptin family of proteases which cleaves the polarly localised OM protein IcsA that is essential for Shigella virulence. Unlike IcsA however, the specific localisation of IcsP on the cell surface is unknown. To determine the distribution of IcsP, a haemagglutinin (HA) epitope was inserted into the non-essential IcsP OM loop 5 using Splicing by Overlap Extension (SOE) PCR, and IcsP(HA) was characterised. Quantum Dot (QD) immunofluorescence (IF) surface labelling of IcsP(HA) was then undertaken. Quantitative fluorescence analysis of S. flexneri 2a 2457T treated with and without tunicaymcin to deplete lipopolysaccharide (LPS) O antigen (Oag) showed that IcsP(HA) was asymmetrically distributed on the surface of septating and non-septating cells, and that this distribution was masked by LPS Oag in untreated cells. Double QD IF labelling of IcsP(HA) and IcsA showed that IcsP(HA) preferentially localised to the new pole of non-septating cells and to the septum of septating cells. The localisation of IcsP(HA) in a rough LPS S. flexneri 2457T strain (with no Oag) was also investigated and a similar distribution of IcsP(HA) was observed. Complementation of the rough LPS strain with rmlD resulted in restored LPS Oag chain expression and loss of IcsP(HA) detection, providing further support for LPS Oag masking of surface proteins. Our data presents for the first time the distribution for the Omptin OM protease IcsP, relative to IcsA, and the effect of LPS Oag masking on its detection.Elizabeth Ngoc Hoa Tran, Matthew Thomas Doyle, Renato Moron
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