2,015 research outputs found
Puncturing of lyophilized tissue engineered vascular matrices enhances the efficiency of their recellularization
Data on in vitro engineered "off the shelf" matrices support the concept of endogenous cellular repopulation driving the graft's remodeling via immune-mediated response. This seems important to further accelerate the cell reconstitution and may play a crucial role when mononuclear cells are used. Nevertheless, studies on decellularized xenogeneic grafts showed only limited host cell repopulation post-implantation. This study aims at a systematic comparison of reseeding methods (dripping, injection, bathing in a cell suspension and combined puncturing-dripping method) to define the most efficient technique enhancing recellularization of tissue engineered vascular matrices (patches, vessels, small diameter and standard size valves) prior implantation. The constructs were analyzed histologically, biochemically and biomechanically. Various preconditioning treatments (wet, lyophilized and air-dried) combined with reseeding methods demonstrated the highest cell loading efficiency, despite applied crimping and flow stress, of lyophilization followed by puncturing-dripping technique. This novel seeding method allows for an efficient, time saving graft reseeding that can be used within a one-step cardiovascular clinical intervention.
STATEMENT OF SIGNIFICANCE
The concept of living tissue engineered, self-repairing, autologous cardiovascular replacements, was proposed alternatively to existing synthetic/xenogeneic prostheses. Recent studies in animal models demonstrate faster in vivo recellularization after grafts pre-seeding with cells prior implantation. Pre-seeded cells hold either, the ability to differentiate directionally or attract host cells, crucial for graft integration and remodeling. It is unclear, however, how efficient the pre-loading is and how well cells withstand the flow. The study presents a systematic overview on cell loading techniques of different cardiovascular constructs, tested under static and dynamic conditions. Comparison illustrates a significantly higher efficiency of cells loading in lyophilized tissues punctured before their standard seeding. This technique may beneficially accelerate remodeling of cardiovascular grafts in further in vivo studies
The MOSFIRE Deep Evolution Field (MOSDEF) Survey: Rest-Frame Optical Spectroscopy for ~1500 H-Selected Galaxies at 1.37 < z < 3.8
In this paper we present the MOSFIRE Deep Evolution Field (MOSDEF) survey.
The MOSDEF survey aims to obtain moderate-resolution (R=3000-3650) rest-frame
optical spectra (~3700-7000 Angstrom) for ~1500 galaxies at 1.37<z<3.80 in
three well-studied CANDELS fields: AEGIS, COSMOS, and GOODS-N. Targets are
selected in three redshift intervals: 1.37<z<1.70, 2.09<z<2.61, and
2.95<z<3.80, down to fixed H_AB (F160W) magnitudes of 24.0, 24.5 and 25.0,
respectively, using the photometric and spectroscopic catalogs from the 3D-HST
survey. We target both strong nebular emission lines (e.g., [OII], Hbeta,
[OIII], 5008, Halpha, [NII], and [SII]) and stellar continuum and absorption
features (e.g., Balmer lines, Ca-II H and K, Mgb, 4000 Angstrom break). Here we
present an overview of our survey, the observational strategy, the data
reduction and analysis, and the sample characteristics based on spectra
obtained during the first 24 nights. To date, we have completed 21 masks,
obtaining spectra for 591 galaxies. For ~80% of the targets we derive a robust
redshift from either emission or absorption lines. In addition, we confirm 55
additional galaxies, which were serendipitously detected. The MOSDEF galaxy
sample includes unobscured star-forming, dusty star-forming, and quiescent
galaxies and spans a wide range in stellar mass (~10^9-10^11.5 Msol) and star
formation rate (~10^0-10^3 Msol/yr). The spectroscopically confirmed sample is
roughly representative of an H-band limited galaxy sample at these redshifts.
With its large sample size, broad diversity in galaxy properties, and wealth of
available ancillary data, MOSDEF will transform our understanding of the
stellar, gaseous, metal, dust, and black hole content of galaxies during the
time when the universe was most active.Comment: Accepted for publication in ApJS; 28 pages, 19 figures; MOSDEF
spectroscopic redshifts available at
http://mosdef.astro.berkeley.edu/Downloads.htm
The MOSDEF survey: a stellar mass-SFR-metallicity relation exists at
We investigate the nature of the relation among stellar mass, star-formation
rate, and gas-phase metallicity (the M-SFR-Z relation) at high redshifts
using a sample of 260 star-forming galaxies at from the MOSDEF
survey. We present an analysis of the high-redshift M-SFR-Z relation based
on several emission-line ratios for the first time. We show that a M-SFR-Z
relation clearly exists at . The strength of this relation is similar
to predictions from cosmological hydrodynamical simulations. By performing a
direct comparison of stacks of and galaxies, we find that
galaxies have dex lower metallicity at fixed M and
SFR. In the context of chemical evolution models, this evolution of the
M-SFR-Z relation suggests an increase with redshift of the mass-loading
factor at fixed M, as well as a decrease in the metallicity of infalling
gas that is likely due to a lower importance of gas recycling relative to
accretion from the intergalactic medium at high redshifts. Performing this
analysis simultaneously with multiple metallicity-sensitive line ratios allows
us to rule out the evolution in physical conditions (e.g., N/O ratio,
ionization parameter, and hardness of the ionizing spectrum) at fixed
metallicity as the source of the observed trends with redshift and with SFR at
fixed M at . While this study highlights the promise of
performing high-order tests of chemical evolution models at high redshifts,
detailed quantitative comparisons ultimately await a full understanding of the
evolution of metallicity calibrations with redshift.Comment: 19 pages, 8 figures, accepted to Ap
The MOSDEF Survey: Kinematic and Structural Evolution of Star-Forming Galaxies at
We present ionized gas kinematics for 681 galaxies at from
the MOSFIRE Deep Evolution Field survey, measured using models which account
for random galaxy-slit misalignments together with structural parameters
derived from CANDELS Hubble Space Telescope (HST) imaging. Kinematics and sizes
are used to derive dynamical masses. Baryonic masses are estimated from stellar
masses and inferred gas masses from dust-corrected star formation rates (SFRs)
and the Kennicutt-Schmidt relation. We measure resolved rotation for 105
galaxies. For the remaining 576 galaxies we use models based on HST imaging
structural parameters together with integrated velocity dispersions and
baryonic masses to statistically constrain the median ratio of intrinsic
ordered to disordered motion, . We find that
increases with increasing stellar mass and decreasing specific SFR (sSFR).
These trends may reflect marginal disk stability, where systems with higher gas
fractions have thicker disks. For galaxies with detected rotation we assess
trends between their kinematics and mass, sSFR, and baryon surface density
(). Intrinsic dispersion correlates most with
and velocity correlates most with mass. By comparing
dynamical and baryonic masses, we find that galaxies at are
baryon dominated within their effective radii (), with Mdyn/Mbaryon
increasing over time. The inferred baryon fractions within ,
, decrease over time, even at fixed mass, size, or surface
density. At fixed redshift, does not appear to vary with
stellar mass but increases with decreasing and increasing
. For galaxies at , the median inferred baryon
fractions generally exceed 100%. We discuss possible explanations and future
avenues to resolve this tension.Comment: Accepted to ApJ. Added Figure 9, corrected sample size (main results
unchanged). 28 pages, 13 figure
Violent and non-violent crimes against sex workers : the influence of the sex market on reporting practices in the United Kingdom
Previous research has shown that sex workers experience extremely high rates of victimization but are often reluctant to report their experiences to the police. This paper explores how the markets in which sex workers operate in the United Kingdom impact upon the violent and non-violent crimes they report to a national support organization and their willingness to report victimization to the police. We use a secondary quantitative data analysis of 2,056 crime reports submitted to the UK National Ugly Mugs (NUM) scheme between 2012 and 2016. The findings indicate that although violence is the most common crime type reported to NUM, sex workers operating in different markets report varying relative proportions of different types of victimization. We also argue that there is some variation in the level of willingness to share reports with the police across the different sex markets, even when the type crime, presence of violence, and other variables are taken into account. Our finding that street sex workers are most likely to report victimization directly to the police challenges previously held assumptions that criminalization is the key factor preventing sex workers from engaging with the police.
Key words: sex work; violence; policing; reported victimizatio
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