Development and BiomechanicalInvestigation of a New Compound Palatal Arch

Abstract. : Background and Aim: : There are many advantages in using a transpalatal arch in orthodontic treatment. In addition to the active movement of individual teeth, such an arch can be used to provide additional anchorage. The aim of this study was to further develop and improve both the clinical and the biomechanical properties of the conventional transpalatal arch. Material and Methods: : Currently available compound material components form an established part of orthodontic therapy. The Compound palatal arch® broadens the scope of this treatment system. The biomechanical effects of the newly developed Compound palatal arch® were verified by comparing them with those of commercially available conventional transpalatal arches. The recently developed Compound palatal arch® is made of one compound element: nickel-titanium/stainless steel. The specific dimensions and design of the nickel-titanium element are aimed at exploiting its superelasticity, even during active molar movement. The biomechanical investigation was carried out using sixcomponent measuring sensors to register the forces and moments in the three spatial dimensions (x, y and z) at 37 °C. The following appliances were investigated: Goshgarian transpalatal arch, quad-helix appliance, Arndt memory expander®, and Compound palatal arch®. Results: : Transpalatal arches could not be inserted without the arch being under tension. This is particularly important when the arch is being used for anchorage purposes. The Goshgarian arch had to be classified as problematic for the movement of single teeth as the load/deflection rate was high. However, this can be utilized for anchorage purposes. The clinical advantage of a quad-helix appliance is the option for asymmetric tooth movement. Conclusions: : This study showed that the quad-helix was effective for both unilateral and bilateral derotation as well as for transversal tooth movement. However, expansions should be confined to ≤ 4 mm, and derotations to < 10°, as forces and moments are otherwise not within the physiologic range. The Arndt memory expander® displayed no advantages over either the quad-helix appliance or the Goshgarian transpalatal arch. Nor did it display any superelastic properties. The linear relationship, the relatively high forces and moments, and the lack of clinical adjustability cast doubts on the benefits of this appliance. The newly developed Compound palatal arch® showed substantial advantages in molar derotation compared with conventional transpalatal arches. Superelastic properties were achieved through the design and positioning of the nickel-titanium element. Expansion with the Compound palatal arch® was comparable with that with conventional transpalatal arches. The clinical advantage is in the fact that this appliance can be reactivated and that dental asymmetries can be treate

Dynamic Functional ForceMeasurements on an Anterior Bite Plane during theNight

Abstract. : Background: : Anterior bite planes are used in removable and fixed appliance treatment. In removable appliance treatment the question arising is whether the delivered forces can achieve active intrusion in terms of their amplitude and duration. In fixed appliance treatment, the force effect on the incisors and associated pathologic side effects, in particular under the application of intrusion mechanics, have to be considered. Subjects and Method: : The aim of the present study was to investigate the effects of an anterior bite plane during the night. For this purpose ten subjects underwent nocturnal sleep investigations by means of a telemetric system. A silicon force sensor was integrated into an anterior bite plane for continuous measurement of bite forces and of the frequency of occlusal contact with the plate. Results: : The occlusal forces exerted on the anterior bite planes ranged between 3 and 80 N. The average forces were 5.5-24 N. The number of occlusal contacts varied between 39 and 558, with forces of between 7 and 9 N being registered in most cases. Major interindividual differences were detected in the magnitude of the force as well as in bite frequency. The intraindividual pattern of arising occlusal forces showed an intermittent force effect. No significant differences were found with regard to gender or growth pattern. Conclusions: : In subjects with removable appliances, no active intrusion of teeth is possible during the night owing to the small number of occlusal contacts. Due to the partially very high forces in fixed appliance therapy, the integration of an anterior bite plane has to be assessed as critical in patients with unfavorable root geometry or bruxis

Corrosion of Orthodontic Pliers Using Different Sterilization Procedures

Abstract : Aim: : The aim of this study was to investigate the corrosion resistance of orthodontic pliers from several manufacturers following different sterilization procedures, namely heat sterilization and the use of a disinfectant agent (Sekusept Extra N®) in an ultrasonic bath. Material and Methods: : Employed in this study were ten distal-end cutters and ten Weingart pliers manufactured by Aesculap®, ETM and Hu-Friedy®, examined under electron microscopy for evidence of corrosion following 500 sterilization cycles. The corrosion data were recorded photographically and graphically. Metallographic microsections were prepared from each pair of pliers and were then analyzed by means of EDX measurements to assess the chemical alloy composition. Results: : The light and electron microscopic evaluation showed that both heat sterilization and high level disinfection caused corrosive changes on the pliers. However, the type of corrosion differed between the two sterilization methods. The main type of corrosion with Sekusept Extra N® was pitting, while with heat sterilization it was surface corrosion. Heat sterilization was the type of corrosion that caused the most corrosive changes, regardless of which type of corrosion was considered. However, these results must be viewed critically, because pitting corrosion is obviously a more severe problem than surface corrosion. In comparing the pliers of various manufacturers, differences were noted. However, it was difficult to rank them when the correct maintenance regime was adhered to. The soldered joint gap was revealed to be a specific weak point, and that area proved to be inadequate due to qualities related to their production, particularly in the Hu-Friedy® pliers. Conclusions: : This study showed that heat sterilization leads to less corrosion than cold disinfection. Corroded pliers can be restored to a useable condition by re-polishing, though it is important that the instructions for their care be strictly adhered t

Linkage Evidence for a Two-Locus Inheritance of LQT-Associated Seizures in a Multigenerational LQT Family With a Novel KCNQ1 Loss-of-Function Mutation

Mutations in several genes encoding ion channels can cause the long-QT (LQT) syndrome with cardiac arrhythmias, syncope and sudden death. Recently, mutations in some of these genes were also identified to cause epileptic seizures in these patients, and the sudden unexplained death in epilepsy (SUDEP) was considered to be the pathologic overlap between the two clinical conditions. For LQT-associated KCNQ1 mutations, only few investigations reported the coincidence of cardiac dysfunction and epileptic seizures. Clinical, electrophysiological and genetic characterization of a large pedigree (n = 241 family members) with LQT syndrome caused by a 12-base-pair duplication in exon 8 of the KCNQ1 gene duplicating four amino acids in the carboxyterminal KCNQ1 domain (KCNQ1dup12; p.R360_Q361dupQKQR, NM_000218.2, hg19). Electrophysiological recordings revealed no substantial KCNQ1-like currents. The mutation did not exhibit a dominant negative effect on wild-type KCNQ1 channel function. Most likely, the mutant protein was not functionally expressed and thus not incorporated into a heteromeric channel tetramer. Many LQT family members suffered from syncopes or developed sudden death, often after physical activity. Of 26 family members with LQT, seizures were present in 14 (LQTplus seizure trait). Molecular genetic analyses confirmed a causative role of the novel KCNQ1dup12 mutation for the LQT trait and revealed a strong link also with the LQTplus seizure trait. Genome-wide parametric multipoint linkage analyses identified a second strong genetic modifier locus for the LQTplus seizure trait in the chromosomal region 10p14. The linkage results suggest a two-locus inheritance model for the LQTplus seizure trait in which both the KCNQ1dup12 mutation and the 10p14 risk haplotype are necessary for the occurrence of LQT-associated seizures. The data strongly support emerging concepts that KCNQ1 mutations may increase the risk of epilepsy, but additional genetic modifiers are necessary for the clinical manifestation of epileptic seizures

Adaptive speciation theory: a conceptual review

Speciation—the origin of new species—is the source of the diversity of life. A theory of speciation is essential to link poorly understood macro-evolutionary processes, such as the origin of biodiversity and adaptive radiation, to well understood micro-evolutionary processes, such as allele frequency change due to natural or sexual selection. An important question is whether, and to what extent, the process of speciation is ‘adaptive’, i.e., driven by natural and/or sexual selection. Here, we discuss two main modelling approaches in adaptive speciation theory. Ecological models of speciation focus on the evolution of ecological differentiation through divergent natural selection. These models can explain the stable coexistence of the resulting daughter species in the face of interspecific competition, but they are often vague about the evolution of reproductive isolation. Most sexual selection models of speciation focus on the diversification of mating strategies through divergent sexual selection. These models can explain the evolution of prezygotic reproductive isolation, but they are typically vague on questions like ecological coexistence. By means of an integrated model, incorporating both ecological interactions and sexual selection, we demonstrate that disruptive selection on both ecological and mating strategies is necessary, but not sufficient, for speciation to occur. To achieve speciation, mating must at least partly reflect ecological characteristics. The interaction of natural and sexual selection is also pivotal in a model where sexual selection facilitates ecological speciation even in the absence of diverging female preferences. In view of these results, it is counterproductive to consider ecological and sexual selection models as contrasting and incompatible views on speciation, one being dominant over the other. Instead, an integrative perspective is needed to achieve a thorough and coherent understanding of adaptive speciation

Effective Disinfection of Orthodontic Pliers

Objective:: Pathogenic microbes may be transmitted directly from the orthodontist to the patient or from the patient to the doctor, and indirectly from patient to patient. The latter may occur via contaminated instruments or surfaces, and is referred to as cross-contamination. The objective of this study was to evaluate the extent of bacterial contamination of orthodontic pliers and the efficacy of the disinfection techniques applied after clinical use. We also sought to examine under standardized conditions the virucidal, bactericidal and fungicidal effects of disinfection techniques used in practice. Materials and Methods:: The efficacy of various disinfection methods was determined after clinical use in-vivo on 10 test subjects and in-vitro with deliberate contamination. The following disinfection methods were tested: 1. Iso-Septol spray 2. Incidur® spray 3. Trough disinfection in combination with 5% Sekusept® Plus solution 4. Ultrasound bath in combination with 5% Sekusept® Plus solution 5. Thermal disinfection For in-vitro contamination we used the test organisms Staphylococcus aureus, Escherichia coli, Candida albicans, Coxsackie virus B4, HSV 1, and Adenovirus type 5. The tests were carried out six to eight times for each organism. The Weingart pliers and distalend cutters were tested. The criteria for effective disinfection were a reduction in infectiosity of five log steps (for bacteria and fungi) or four log steps (viruses). Statistical analysis was carried out using the Wilcoxon and Whitney U-test. Results:: The presence of contamination following clinical use was not adequately eliminated with all disinfection methods. The spray methods exhibited shortcomings in disinfection. For the type of contamination defined, trough disinfection with 5% Sekusept® Plus and the Incidur® and Iso-Septol spray disinfection methods provided insufficient disinfection. Conversely, the ultrasound bath with 5% Sekusept® Plus solution and steam disinfection met the criteria for effective disinfection for all microbes. No statistically significant difference was found between the oiled and unoiled states. In some cases, there were slightly higher rates of contamination with the Weingart pliers as with the distalend cutters. However, these were not statistically significant. Conclusions:: It should be possible to disinfect lipophilic viruses and the usual bacterial infections adequately with all methods, provided that the use of sprays and trough disinfection is preceded by cleaning with brush and water, followed by drying. With hydrophilic viruses, however, the spray and trough disinfection methods are limited in their efficacy and cannot be considered adequate. Exclusively chemical methods are therefore less effective than thermal or physical-chemical methods. Thermal disinfection and the ultrasound bath in combination with 5% Sekusept® Plus are clearly superior to spray disinfection and trough disinfection alone. The ultrasound bath and thermal disinfection can therefore be recommended for the disinfection of orthodontic pliers. We recommend that the pliers be cleaned beforehand due to their uneven surface

Competition dynamics in a chemical system of self-replicating macrocycles

Central to the origin of life is the question how a chemical system transitioned from interacting molecules to an entity with the capacity for self-replication, diversification and adaptive evolution. Here, we study a chemical system that is comprised of macrocycles that have been shown to spontaneously give rise to self-replicating entities. By combining experimental and theoretical approaches, we strive to understand the evolutionary potential of this system. In particular, we apply eco-evolutionary reasoning to investigate whether and when this system of chemical replicators can diversify. Here, we report first results of a simplified stochastic chemical reaction model that is parameterized on the basis of experimental data. The model considers the competition of two replicators that do not interact directly but need similar building blocks for their growth and reproduction. Interestingly, the replicator that emerges first is being overtaken by the later one. By means of stochastic simulations, we will explore how the competitive ability of a replicator is determined by its chemical characteristics, and under which conditions replicators can coexist. The results will subsequently inform the design of future experiments

Modulation of the Tumor-Associated Immuno-Environment by Non-Invasive Physical Plasma

Over the past 15 years, investigating the efficacy of non-invasive physical plasma (NIPP) in cancer treatment as a safe oxidative stress inducer has become an active area of research. So far, most studies focused on the NIPP-induced apoptotic death of tumor cells. However, whether NIPP plays a role in the anti-tumor immune responses need to be deciphered in detail. In this review, we summarized the current knowledge of the potential effects of NIPP on immune cells, tumor–immune interactions, and the immunosuppressive tumor microenvironment. In general, relying on their inherent anti-oxidative defense systems, immune cells show a more resistant character than cancer cells in the NIPP-induced apoptosis, which is an important reason why NIPP is considered promising in cancer management. Moreover, NIPP treatment induces immunogenic cell death of cancer cells, leading to maturation of dendritic cells and activation of cytotoxic CD8+ T cells to further eliminate the cancer cells. Some studies also suggest that NIPP treatment may promote anti-tumor immune responses via other mechanisms such as inhibiting tumor angiogenesis and the desmoplasia of tumor stroma. Though more evidence is required, we expect a bright future for applying NIPP in clinical cancer management

Thin films with implemented molecular switches for the application in polymer-based optical waveguides

Complexes like iron (II)-triazoles exhibit spin crossover behavior at ambient temperature and are often considered for possible application. In previous studies, we implemented complexes of this type into polymer nanofibers and first polymer-based optical waveguide sensor systems. In our current study, we synthesized complexes of this type, implemented them into polymers and obtained composites through drop casting and doctor blading. We present that a certain combination of polymer and complex can lead to composites with high potential for optical devices. For this purpose, we used two different complexes [Fe(atrz)3](2ns)2 and [Fe(atrz)3]Cl1.5(BF4)0.5 with different polymers for each composite. We show through transmission measurements and UV/VIS spectroscopy that the optical properties of these composite materials can reversibly change due to the spin crossover effect