The Potential of Whey Protein in Food Packaging

Whey protein has great potential to produce biodegradable and edible packaging. A broader spectrum of properties, namely antioxidant and antimicrobial, can be achieved through the incorporation of substances, such as essential oils, intended to be released during food storage, as an active system.info:eu-repo/semantics/publishedVersio

Evaluation of the oxidative status of salami packaged with an active whey protein film

Active packaging aims to prolong food's shelf-life by directly interacting with the packaged food. This type of packaging is characterized by having the active agent incorporated into the package polymer, such as antioxidant additives, that will gradually migrate from the package polymer to the packed food and, consequently, delay food's natural lipid oxidation. In this study, the efficiency of an active whey protein film incorporated with a rosemary extract on retarding the lipid oxidation of salami slices was evaluated. The lipid oxidation of the salami was measured by the thiobarbituric acid reactive substances (TBARS) assay and hexanal monitorization. Also, a sensory analysis on the salami packaged for 60 and 90 days was performed. The active film was able to delay the salami's lipid oxidation for, at least, 30 days. The samples packaged with the active film revealed a bitter taste related to the rosemary extract and a bit sweet from the WP and the glycerol.This work was supported by the research project “Development of an edible film based on whey protein with antioxidant and antimicrobial activity using essential oils” (2012DAN807) funded by the National Institute of Health Doutor Ricardo Jorge, I.P. (Lisbon, Portugal).info:eu-repo/semantics/publishedVersio

Mediation of information in librarian job and its process in university libraries within cultural actions scope

In a scenario where the information is considered basic inputs needed for the development of individuals working in the society as well as an element for the development of a society based on economic growth, political and scientific, it seems necessary to think, even for the purpose test, meaning that most closely matches what you want to understand why information and ways of operationalizing this concept within the context of information science. As a way to explain this operation, this article seeks to ascertain to what extent the action of mediation of information made by the librarian of the university library provides the recovery and transformation of the socio-cultural community which serves to promote both consumption and also the production of culture. This attribution of importance to make the librarian is tied to how these professional understand and internalize the concept of information to direct their practice. Thus to achieve the proposed objectives, we start from the concept of information and mediation of information until we get to the concepts of culture, cultural action and cultural activities.Em um cenário em que a informação é considerada insumo básico necessário para o desenvolvimento de sujeitos atuantes na sociedade, bem como um elemento para o desenvolvimento de uma sociedade calcada no crescimento econômico, político e científico, parece-nos necessário pensar, mesmo que para efeito de ensaio, um significado que melhor se aproxime do que se quer entender por informação e as formas de operacionalização deste conceito dentro do contexto da Ciência da Informação. Como forma de explicitar esta operacionalização, pretende-se neste artigo verificar em que medida a ação de Mediação da Informação efetuada pelo profissional bibliotecário de biblioteca universitária proporciona a valorização e transformação do espaço sociocultural da comunidade a qual atende ao promover tanto o consumo como também a produção de cultura. Essa atribuição de importância ao fazer do bibliotecário está atrelada a como esse profissional entende e internaliza o conceito de informação para dirigir sua prática. Desta forma, para alcançar os objetivos propostos, partimos do conceito de informação e Mediação da Informação até chegarmos aos conceitos de cultura, ação cultural e animação cultural

Surface Modification of Bioresorbable Phosphate Glasses for Controlled Protein Adsorption

The traditional silicate bioactive glasses exhibit poor thermal processability, which inhibits fiber drawing or sintering into scaffolds. The composition of the silicate glasses has been modified to enable hot processing. However, the hot forming ability is generally at the expense of bioactivity. Metaphosphate glasses, on the other hand, possess excellent thermal processability, congruent dissolution, and a tailorable degradation rate. However, due to the layer-by-layer dissolution mechanism, cells do not attach to the material surface. Furthermore, the congruent dissolution leads to a low density of OH groups forming on the glass surface, limiting the adsorption of proteins. It is well regarded that the initial step of protein adsorption is critical as the cells interact with this protein layer, rather than the biomaterial itself. In this paper, we explore the possibility of improving protein adsorption on the surface of phosphate glasses through a variety of surface treatments, such as washing the glass surface in acidic (pH 5), neutral, and basic (pH 9) buffer solutions followed or not by a treatment with (3-aminopropyl)triethoxysilane (APTS). The impact of these surface treatments on the surface chemistry (contact angle, ζ-potential) and glass structure (FTIR) was assessed. In this manuscript, we demonstrate that understanding of the material surface chemistry enables to selectively improve the adsorption of albumin and fibronectin (used as model proteins). Furthermore, in this study, well-known silicate bioactive glasses (i.e., S53P4 and 13-93) were used as controls. While surface treatments clearly improved proteins adsorption on the surface of both silicate and phosphate glasses, it is of interest to note that protein adsorption on phosphate glasses was drastically improved to reach similar protein grafting ability to the silicate bioactive glasses. Overall, this study demonstrates that the limited cell/phosphate glass biological response can easily be overcome through deep understanding and control of the glass surface chemistry

SRC inhibition prevents P-cadherin mediated signaling and function in basal-like breast cancer cells

BACKGROUND: Basal-like breast cancer (BLBC) is a poor prognosis subgroup of triple-negative carcinomas that still lack specific target therapies and accurate biomarkers for treatment selection. P-cadherin is frequently overexpressed in these tumors, promoting cell invasion, stem cell activity and tumorigenesis by the activation of Src-Family kinase (SRC) signaling. Therefore, our aim was to evaluate if the treatment of BLBC cells with dasatinib, the FDA approved SRC inhibitor, would impact on P-cadherin induced tumor aggressive behavior. METHODS: P-cadherin and SRC expression was evaluated in a series of invasive Breast Cancer and contingency tables and chi-square tests were performed. Cell-cell adhesion measurements were performed by Atomic Force Microscopy, where frequency histograms and Gaussian curves were applied. 2D and 3D cell migration and invasion, proteases secretion and self-renew potential were evaluated in vitro. Student's t-tests were used to determine statistically significant differences. The cadherin/catenin complex interactions were evaluated by in situ proximity-ligation assay, and statistically significant results were determined by using Mann-Whitney test with a Bonferroni correction. In vivo xenograft mouse models were used to evaluate the impact of dasatinib on tumor growth and survival. ANOVA test was used to evaluate the differences in tumor size, considering a confidence interval of 95%. Survival curves were estimated by the Kaplan-Meier's method, using the log-rank test to assess significant differences for mice overall survival. RESULTS: Our data demonstrated that P-cadherin overexpression is significantly associated with SRC activation in breast cancer cells, which was also validated in a large series of primary tumor samples. SRC activity suppression with dasatinib significantly prevented the in vitro functional effects of P-cadherin overexpressing cells, as well as their in vivo tumorigenic and metastatic ability, by increasing mice overall survival. Mechanistically, SRC inhibition affects P-cadherin downstream signaling, rescues the E-cadherin/p120-catenin complex to the cell membrane, recovering cell-cell adhesion function. CONCLUSIONS: In conclusion our findings show that targeting P-cadherin/SRC signaling and functional activity may open novel therapeutic opportunities for highly aggressive and poor prognostic basal-like breast cancer.This work was funded by Laço Grant 2014, by FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by FCT - Fundação para a Ciência e a Tecnologia/ Ministério da Ciência, Tecnologia e Ensino Superior under the projects PTDC/SAU-GMG/120049/ 2010-FCOMP-01-0124-FEDER-021209, PEst-C/SAU/LA0003/2013, NORTE-01- 0145-FEDER-000029 and POCI-01-0145-FEDER-016390. FCT funded the research grants of ASR (SFRH/BPD/75705/2011), ARN (SFRH/BD/100380/2014), BS (SFRH/ BPD/104208/2014), AFV (SFRH/BPD/90303/2012), as well as JP with Programa IFCT 2013 (FCT Investigator). IPATIMUP integrates the i3S Research Unit, which is partially supported by FCT in the framework of the project “Institute for Research and Innovation in Health Sciences” (POCI-01-0145-FEDER-007274)

Projeto Balde Cheio: Transferência de tecnologia na produção leiteira - Estudo de caso do sítio São José, de Nhandeara, SP.

bitstream/CPPSE/16834/1/comutecnico74.pd

Projeto Balde Cheio: Transferência de tecnologia na produção leiteira - Estudo de caso do sítio São José, de Nhandeara, SP.

bitstream/CPPSE/16905/1/comutecnico74.pdfISSN 1981-206

Dinâmica da agricultura anual na região do Matopiba.

Considerando a importância de se conhecer a dinâmica do uso e cobertura da terra da região, este estudo mapeou a prática da agricultura anual com emprego de alta tecnologia, nos anos de 2005, 2010 e 2014, e estimou as taxas de expansão desta atividade nos diferentes períodos.SBSR 2015