Leverage and Profitability Evolution Analysis of the POP-ART, s.r.o. Company

Import 22/07/2015Cílem bakalářské práce je zhodnotit vývoj zadluženosti a rentability společnosti POP-ART, s.r.o. v letech 2009 – 2013 a navrhnout určitá doporučení společnosti.The aim of this thesis is to evaluate the evolution of the debt and the profitability of the company POP -ART s.r.o. in the years 2009 - 2013 and to propose specific recommendations to the company.154 - Katedra financívýborn

Comparison of communication nets and programming methods fo PLC Simatic

Tato práce pojednává o možnostech prostředí STEP7, co se týče použitelných sítí a programovacích jazyků. Práce v první kapitole obsahuje popis různých standardů sítí včetně ukázky jejích hardwarové konfigurace. Druhá kapitola pojednává o jednotlivých programovacích jazycích obsažených v prostředí STEP7 a demonstruje jejich užití na cvičných úlohách.This thesis deals with software development kit STEP7 and its posibilities in using different networking and programing options. First chapter contains descriptions of different network standards and example of thein configuration. Sekond chapter deals with programing laguages in STEP7 and demonstrates thein use on various examples.

Modifying the Processes of Employee Selection and Adaptation in a Chosen Organization

Cílem diplomové práce je na základě analýzy stávajících procesů výběru a adaptace zaměstnanců ve společnosti ČSOB a.s. doporučit návrhy na jeho zlepšení. Diplomová práce je zaměřena na proces výběru a adaptace zaměstnanců na pozici Klientský pracovník.The aim of this thesis is to analyze existing processes of employee selection and adaptation in company ČSOB a.s. and recommend a suggestions for improvement. The diploma thesis is focused on the process of selection and adaptation of employees to the position of Client service.115 - Katedra managementuvýborn

Design and Optimization of Tool Production for Shaping Specific Internal Gearing

Tato bakalářská práce se zabývá problematikou návrhu a výroby nástrojů. Hlavním cílem této práce je navrhnout nástroj pro vnitřní spirálové ozubení, navrhnout technologický postup výroby nože a optimalizovat jeho výrobu. Práce obsahuje čtyři hlavní části. První část je rešeršní a zabývá se problematikou návrhu a výrobou nástrojů. Tato část je zaměřena na jednotlivé části konstrukce nástroje a možnosti jeho výroby. V druhé části je návrh a technologický postup výroby monolitního obrážecího nože. V třetí části je optimalizace nástroje, pojata z hlediska konstrukce. Je zde navrhnuto těleso nástroje pro vyměnitelnou břitovou destičku a taktéž vytvořen technologický postup. Poslední část obsahuje technicko-ekonomické zhodnocení, ve kterém jsou porovnány oba typy nástrojů.This Bachelor Thesis deals with the design and production of tools. The main goal of this work is to design a tool for internal spiral gearing, to design the technological process of tool production and to optimize production. The work contains four main parts. The first part is a research and deals with the issue of design and production of tools. This part is focused on individual parts of the tool construction and the possibilities of its production. The second part is the design ad technological process of a slotting tool. In the third part, the tools are optimized, conceived in terms of construction also it contains design body tool for a tipped tool and created technological procedure. The last part contains technical and economic evaluation, in which both types of tools are compared.346 - Katedra obrábění, montáže a strojírenské metrologievýborn

Supplementary data for article: Samiec, P.; Švorc, Ľ.; Stanković, D. M.; Vojs, M.; Marton, M.; Navrátilová, Z. Mercury-Free and Modification-Free Electroanalytical Approach towards Bromazepam and Alprazolam Sensing: A Facile and Efficient Assay for Their Quantification in Pharmaceuticals Using Boron-Doped Diamond Electrodes. Sensors and Actuators, B: Chemical 2017, 245, 963–971. https://doi.org/10.1016/j.snb.2017.02.023

Supplementary material for: [https://doi.org/10.1016/j.snb.2017.02.023]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2442]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3080

Supplementary data for article: Samiec, P.; Švorc, Ľ.; Stanković, D. M.; Vojs, M.; Marton, M.; Navrátilová, Z. Mercury-Free and Modification-Free Electroanalytical Approach towards Bromazepam and Alprazolam Sensing: A Facile and Efficient Assay for Their Quantification in Pharmaceuticals Using Boron-Doped Diamond Electrodes. Sensors and Actuators, B: Chemical 2017, 245, 963–971. https://doi.org/10.1016/j.snb.2017.02.023

Supplementary material for: [https://doi.org/10.1016/j.snb.2017.02.023]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2442]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3080

The production of pig preimplantation embryos in vitro: Current progress and future prospects

Human assisted reproductive technology procedures are routinely performed in clinics globally, and some of these approaches are now common in other mammals such as cattle. This is currently not the case in pigs. Given that the global population is expected to increase by over two billion people between now and 2050, the demand for meat will also undoubtedly increase. With this in mind, a more sustainable way to produce livestock; increasing productivity and implementing methods that will lead to faster genetic selection, is imperative. The establishment of routine and production scale pig embryo in vitro production could be a solution to this problem. Producers would be able to increase the overall number of offspring born, animal transportation would be more straightforward and in vitro produced embryos could be produced from the gametes of selected elite. Here we review the most recent developments in pig embryology, outline the current barriers and key challenges that exist, and outline research priorities to surmount these difficulties

Association of genetic variants in the promoter region of genes encoding p22phox (CYBA) and glutamate cysteine ligase catalytic subunit (GCLC) and renal disease in patients with type 1 diabetes mellitus

<p>Abstract</p> <p>Background</p> <p>Oxidative stress is recognized as a major pathogenic factor of cellular damage caused by hyperglycemia. NOX/NADPH oxidases generate reactive oxygen species and NOX1, NOX2 and NOX4 isoforms are expressed in kidney and require association with subunit p22phox (encoded by the <it>CYBA </it>gene). Increased expression of p22phox was described in animal models of diabetic nephropathy. In the opposite direction, glutathione is one of the main endogenous antioxidants whose plasmatic concentrations were reported to be reduced in diabetes patients. The aim of the present investigation was to test whether functional single nucleotide polymorphisms (SNPs) in genes involved in the generation of NADPH-dependent O₂^•-(-675 T → A in <it>CYBA</it>, unregistered) and in glutathione metabolism (-129 C → T in <it>GCLC </it>[rs17883901] and -65 T → C in <it>GPX3 </it>[rs8177412]) confer susceptibility to renal disease in type 1 diabetes patients.</p> <p>Methods</p> <p>401 patients were sorted into two groups according to the presence (n = 104) or absence (n = 196) of overt diabetic nephropathy or according to glomerular filtration rate (GFR) estimated by Modification of Diet in Renal Disease (MDRD) equation: ≥ 60 mL (n = 265) or < 60 mL/min/1.73 m²(n = 136) and were genotyped.</p> <p>Results</p> <p>No differences were found in the frequency of genotypes between diabetic and non-diabetic subjects. The frequency of GFR < 60 mL/min was significantly lower in the group of patients carrying <it>CYBA </it>genotypes T/A+A/A (18.7%) than in the group carrying the T/T genotype (35.3%) (P = 0.0143) and the frequency of GFR < 60 mL/min was significantly higher in the group of patients carrying <it>GCLC </it>genotypes C/T+T/T (47.1%) than in the group carrying the C/C genotype (31.1%) (<it>p </it>= 0.0082). Logistic regression analysis identified the presence of at least one A allele of the <it>CYBA </it>SNP as an independent protection factor against decreased GFR (OR = 0.38, CI95% 0.14-0.88, <it>p </it>= 0.0354) and the presence of at least one T allele of the <it>GCLC </it>rs17883901 SNP as an independent risk factor for decreased GFR (OR = 2.40, CI95% 1.27-4.56, <it>p </it>= 0.0068).</p> <p>Conclusions</p> <p>The functional SNPs <it>CYBA </it>-675 T → A and <it>GCLC </it>rs17883901, probably associated with cellular redox imbalances, modulate the risk for renal disease in the studied population of type 1 diabetes patients and require validation in additional cohorts.</p

Sulforaphane restores cellular glutathione levels and reduces chronic periodontitis neutrophil hyperactivity in vitro

The production of high levels of reactive oxygen species by neutrophils is associated with the local and systemic destructive phenotype found in the chronic inflammatory disease periodontitis. In the present study, we investigated the ability of sulforaphane (SFN) to restore cellular glutathione levels and reduce the hyperactivity of circulating neutrophils associated with chronic periodontitis. Using differentiated HL60 cells as a neutrophil model, here we show that generation of extracellular O2 . - by the nicotinamide adenine dinucleotide (NADPH) oxidase complex is increased by intracellular glutathione depletion. This may be attributed to the upregulation of thiol regulated acid sphingomyelinase driven lipid raft formation. Intracellular glutathione was also lower in primary neutrophils from periodontitis patients and, consistent with our previous findings, patients neutrophils were hyper-reactive to stimuli. The activity of nuclear factor erythroid-2-related factor 2 (Nrf2), a master regulator of the antioxidant response, is impaired in circulating neutrophils from chronic periodontitis patients. Although patients' neutrophils exhibit a low reduced glutathione (GSH)/oxidised glutathione (GSSG) ratio and a higher total Nrf2 level, the DNA-binding activity of nuclear Nrf2 remained unchanged relative to healthy controls and had reduced expression of glutamate cysteine ligase catalytic (GCLC), and modifier (GCLM) subunit mRNAs, compared to periodontally healthy subjects neutrophils. Pre-treatment with SFN increased expression of GCLC and GCM, improved intracellular GSH/GSSG ratios and reduced agonist-activated extracellular O2 . - production in both dHL60 and primary neutrophils from patients with periodontitis and controls. These findings suggest that a deficiency in Nrf2-dependent pathways may underpin susceptibility to hyper-reactivity in circulating primary neutrophils during chronic periodontitis. © 2013 Dias et al

Bioreducible Liposomes for Gene Delivery: From the Formulation to the Mechanism of Action

BACKGROUND: A promising strategy to create stimuli-responsive gene delivery systems is to exploit the redox gradient between the oxidizing extracellular milieu and the reducing cytoplasm in order to disassemble DNA/cationic lipid complexes (lipoplexes). On these premises, we previously described the synthesis of SS14 redox-sensitive gemini surfactant for gene delivery. Although others have attributed the beneficial effects of intracellular reducing environment to reduced glutathione (GSH), these observations cannot rule out the possible implication of the redox milieu in its whole on transfection efficiency of bioreducible transfectants leaving the determinants of DNA release largely undefined. METHODOLOGY/PRINCIPAL FINDINGS: With the aim of addressing this issue, SS14 was here formulated into binary and ternary 100 nm-extruded liposomes and the effects of the helper lipid composition and of the SS14/helper lipids molar ratio on chemical-physical and structural parameters defining transfection effectiveness were investigated. Among all formulations tested, DOPC/DOPE/SS14 at 25:50:25 molar ratio was the most effective in transfection studies owing to the presence of dioleoyl chains and phosphatidylethanolamine head groups in co-lipids. The increase in SS14 content up to 50% along DOPC/DOPE/SS14 liposome series yielded enhanced transfection, up to 2.7-fold higher than that of the benchmark Lipofectamine 2000, without altering cytotoxicity of the corresponding lipoplexes at charge ratio 5. Secondly, we specifically investigated the redox-dependent mechanisms of gene delivery into cells through tailored protocols of transfection in GSH-depleted and repleted vs. increased oxidative stress conditions. Importantly, GSH specifically induced DNA release in batch and in vitro. CONCLUSIONS/SIGNIFICANCE: The presence of helper lipids carrying unsaturated dioleoyl chains and phosphatidylethanolamine head groups significantly improved transfection efficiencies of DOPC/DOPE/SS14 lipoplexes. Most importantly, this study shows that intracellular GSH levels linearly correlated with transfection efficiency while oxidative stress levels did not, highlighting for the first time the pivotal role of GSH rather than oxidative stress in its whole in transfection of bioreducible vectors