Use of satellite images for broad-scale modelling of conservation areas for wolves in the Carpathian Mountains, central Europe

This study analysed the spatial structure of the Carpathian Mountains, in Central Europe, considering it a unit that extends across national boundaries, and assessing the suitability of areas were wolves could be conserved. Physical characteristics of the area were extracted from NOAA-AVHRR NDVI. A set of 9 images from different periods of the year was used to parameterise the phenological variability of the area. Digital maps of road networks, human settlements and a DEM were integrated in a GIS. Locations of wolf presence were used to extract “optimal” environmental characteristics that served as reference for estimating the degree of suitability over the whole area. Results show that most of the Carpathian Mountains are highly suitable for the wolf and that highly suitable areas are actually inhabited by the present population of wolf. These are also the area most phenologically stable

Wavelength-dependent spatial variation in the reflectance of 'homogeneous' ground calibration targets (Paper presented at XIX ISPRS Congress, 16-22 July, 2000, Amsterdam, The Netherlands)

Remotely sensed data are most useful if calibrated to spectral reflectance of known features. One simple method of calibration is regression of remote data on the reflectance of several ground targets as measured in the field, the so called empirical line method (ELM). The ideal situation would be one where a range of ground targets representing all the features of interest in the remote image were available for ground measurements (Lawless et al., 1998). The identification of suitable ground targets is constrained by several limitations, such as their size (to minimise edge effects), their absolute reflectance (to represent spectral characteristics of the image) and their effective spatial variability (to extract reflectance characteristics representative of the target). The size of a ground target is dependent on the spatial resolution of the image that must be calibrated (Justice & Townshend, 1981) and the number of observations needed to represent features in the image has been suggested to depend upon the spatial resolution of the remotely sensed image (Justice & Townshend, 1981) and on the spatial variability of the ground target (Harlan et al., 1979; Curran & Williamson, 1986). Although ground targets used for calibration should be spectrally “bland” and spatially uniform by definition (Clark et al., 1999), it is sometimes very difficult to find such places available for calibrating remotely sensed images. When surfaces that apparently satisfy these conditions are available in suitable size, their sampling needs to be designed to optimise representation of the whole surface and available resources (e.g., effort and time). Surfaces that look spatially uniform by eye may actually contain spatial variation, and this spatial variation may depends on wavelength (Atkinson & Emery, 1999). Such variability can be detected using geostatistics, which is concerned with issues such as spatial correlation and analyses of spatial data. Geostatistical tools have been used in a variety of studies and the variogram has been applied in remote sensing and ecology to design optimal sampling strategies for variables sampled in space (Atkinson, 1991; Rossi et al., 1992) and time (Salvatori et al., 1999). This study investigates the spatial variability of potentially suitable ground calibration targets (GCT) using a geostatistical approach, which gives results that can be used to design optimal sampling strategies for such surfaces. The targets were selected from an area where an Itres Instruments Compact Airborne Spectral Imager (casi) with ground resolution of about 1.5 metres was flown at the same time as ground data were acquired

A role for independent peer review: reflections on the gateway review process

Healthcare organisations face constant pressure to change in order to pursue quality and performance improvements. As a result, professionals are often said to be experiencing change fatigue. This can be problematic for policy implementation initiatives. This paper reflects on an informal Gateway Review of Leicester, Leicestershire and Rutland's Integrated Point of Access Programme to make the case for independent peer review of healthcare management change programmes in order to work towards reducing change fatigue and its impacts. Peer review has been identified as an effective but underused process across health and social care provision and management. With this in mind, the Gateway Review outlined here is used to exemplify some of the practical benefits and challenges of external peer review. Based on this experience, a number of learning points and recommendations are outlined for those undertaking or thinking about undertaking peer review in healthcare management

Inflammatory Responses and Barrier Function of Endothelial Cells Derived from Human Induced Pluripotent Stem Cells

Several studies have reported endothelial cell (EC) derivation from human induced pluripotent stem cells (hiPSCs). However, few have explored their functional properties in depth with respect to line-to-line and batch-to-batch variability and how they relate to primary ECs. We therefore carried out accurate characterization of hiPSC-derived ECs (hiPSC-ECs) from multiple (non-integrating) hiPSC lines and compared them with primary ECs in various functional assays, which included barrier function using real-time impedance spectroscopy with an integrated assay of electric wound healing, endothelia-leukocyte interaction under physiological flow to mimic inflammation and angiogenic responses in in vitro and in vivo assays. Overall, we found many similarities but also some important differences between hiPSC-derived and primary ECs. Assessment of vasculogenic responses in vivo showed little difference between primary ECs and hiPSC-ECs with regard to functional blood vessel formation, which may be important in future regenerative medicine applications requiring vascularization. In this article, Orlova and colleagues show that hiPSC-ECs have similar features to primary ECs but also show some differences. hiPSC-ECs exhibited higher barrier function, lower expression of pro-inflammatory adhesive receptors, and more stringent stromal cell requirements. Importantly, healthy control CD31+ hiPSC-ECs showed high consistency between different batches and lines, forming a good basis for disease modeling applications

Could the periodontal therapy improve the cardiologic patient health? A narrative review

Background: Cardiovascular diseases (CVD) is the major cause of mortality globally, with increasing evidence suggesting a link between periodontitis, and CVD. This study aims to explore the association between periodontitis and CVD, and the impact of periodontal therapy on cardiovascular health. Methods: This review synthesized findings from preclinical and clinical studies, without publication year restrictions, examining periodontitis and CVD through various lenses. Scientific databases were inspected with keywords related to periodontitis and CVD. Results: The review identifies a substantial association between periodontitis and an increased risk of several CVD, supported by both epidemiological and interventional studies. Results suggest the complexity of the relationship, influenced by factors like the severity of periodontitis and the presence of other systemic conditions. Clinical data indicates that periodontal therapy, particularly non -surgical periodontal therapy, may reduce systemic inflammatory markers and thus may play a role in the primary and secondary prevention of CVD events, highlighting the potential of periodontal therapy to not only maintain oral health but also to modulate cardiovascular risk factors. Conclusions: Current evidence supports a significant association between periodontitis and increased cardiovascular risk, promoting integrated healthcare approaches that consider oral health as a key -component of cardiovascular care and wellbeing

A new bioactive glass/collagen hybrid composite for applications in dentistry

Bioactive glasses (BGs) are currently employed in a wide range of medical and dentistry applications by virtue of their bone-bonding ability. The incorporation of BGs into a collagen matrix may be used to combine the regenerative potential of these materials with the specific biological advantages of collagen. However, most of the collagen/BG composites reported in the literature are scaffolds and there is a lack of moldable putties or injectable systems. Here, granules of an innovative BG containing strontium and magnesium were mixed with collagen and PEG to obtain a putty (BGMS/C) suitable for dental applications. For the sake of comparison, granules of 45S5 Bioglass\uae, the gold standard among BGs, were used to prepare a 45S5/collagen putty. Both the composites were evaluated in vitro with respect to murine fibroblasts. The materials showed an excellent biocompatibility, making them interesting for possible applications in dentistry and reconstructive surgery. Moreover, BGMS/C seems to stimulate cell proliferation.Bioactive glasses (BGs) are currently employed in a wide range of medical and dentistry applications by virtue of their bone-bonding ability. The incorporation of BGs into a collagen matrix may be used to combine the regenerative potential of these materials with the specific biological advantages of collagen. However, most of the collagen/BG composites reported in the literature are scaffolds and there is a lack of moldable putties or injectable systems. Here, granules of an innovative BG containing strontium and magnesium were mixed with collagen and PEG to obtain a putty (BGMS/C) suitable for dental applications. For the sake of comparison, granules of 45S5 Bioglass\uae, the gold standard among BGs, were used to prepare a 45S5/collagen putty. Both the composites were evaluated in vitro with respect to murine fibroblasts. The materials showed an excellent biocompatibility, making them interesting for possible applications in dentistry and reconstructive surgery. Moreover, BGMS/C seems to stimulate cell proliferation

Tissue-specific deregulation of selected HDACs characterizes ALS progression in mouse models: pharmacological characterization of SIRT1 and SIRT2 pathways

Acetylation homeostasis is thought to play a role in amyotrophic lateral sclerosis, and treatment with inhibitors of histone deacetylases has been considered a potential and attractive therapeutic approach, despite the lack of a thorough study of this class of proteins. In this study, we have considerably extended previous knowledge on the expression of 13 histone deacetylases in tissues (spinal cord and muscle) from mice carrying two different ALS-linked SOD1 mutations (G93A-SOD1 and G86R-SOD1). We have then focused on class III histone deacetylases SIRT1 and SIRT2 that are considered relevant in neurodegenerative diseases. SIRT1 decreases in the spinal cord, but increases in muscle during the progression of the disease, and a similar expression pattern is observed in the corresponding cell models (neuroblastoma and myoblasts). SIRT2 mRNA expression increases in the spinal cord in both G93A-SOD1 and G86R-SOD1 mice but protein expression is substantially unchanged in all the models examined. At variance with other sirtuin modulators (sirtinol, AGK2 and SRT1720), the well-known SIRT1 inhibitor Ex527 has positive effects on survival of neuronal cells expressing mutant SOD1, but this effect is neither mediated by SIRT1 inhibition nor by SIRT2 inhibition. These data call for caution in proposing sirtuin modulation as a target for treatment

Minimally invasive treatment of benign complete stenosis of colorectal anastomosis

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Next-to-leading-order QCD Corrections to Higgs Production in association with a Jet

We compute the next-to-leading-order (NLO) QCD corrections to the Higgs pT distribution in Higgs production in association with a jet via gluon fusion at the LHC, with exact dependence on the mass of the quark circulating in the heavy-quark loops. The NLO corrections are presented including the top-quark mass, and for the first time, the bottom-quark mass as well. Further, besides the on-shell mass scheme, we consider for the first time a running mass renormalisation scheme. The computation is based on amplitudes which are valid for arbitrary heavy-quark masses.Comment: LaTeX, 7 pages, 5 figure