High performance thin-layer chromatography (HPTLC) in the quality control of herbal products

Podeu consultar el llibre complet a: http://hdl.handle.net/2445/128014The introduction of high performance thin-layer chromatography (HPTLC) for quality control of herbal products, using standardised methodology and system suitability tests for the qualification of the plates, has improved reproducibility. The use of intensity markers implemented by the Ph. Eur. improved the description and interpretation of the chromatograms. Quantitative information can be retrieved from the electronic images of the chromatograms and used for comprehensive HPTLC fingerprinting: a single HPTLC analysis gives information on identity, purity and content of an herbal drug/preparation/product, simplifying the quality control

Evidencia clínica de STW 5 (Iberogast®) en patología digestiva

La dispepsia funcional (DF) y el síndrome del intestino irritable (SII) son las alteraciones gastrointestinales funcionales más frecuentes. Requieren un tratamiento multifactorial ya que su aparición se relaciona con diversas afecciones funcionales, como alteraciones de la motilidad, hipersensibilidad gastrointestinal, inflamación, alteración de la microbiota intestinal, estrés y trastornos psicológicos, entre otros. SWT 5 es una combinación de 9 extractos vegetales con actividades sinérgicas y complementarias que es capaz de modular la motilidad gástrica e intestinal, inhibe la secreción ácida gástrica, tiene actividad antiinflamatoria y protectora de la mucosa, y reduce la hipersensibilidad intestinal. Desde 1990, se ha demostrado una eficacia estadísticamente significativa en 6 ensayos clínicos aleatorizados, a doble ciego y controlados: cinco en DF (4 frente a placebo y uno frende a cisaprida) y uno en SII (frente a placebo). La eficacia en DF y SII también se ha mostrado en estudios observacionales que involucran más de 5000 adultos y 44000 niños tratados con STW 5. La combinación de extractos presenta una excelente tolerabilidad y un buen perfil de seguridad, con una incidencia de efectos adversos muy baja. Palabras clave Dispepsia funcional, síndrome del intestino irritable, malestar digestivo, STW 5, Iberis amara, manzanilla común, angélica, alcaravea, cardo mariano, celidonia, melisa, menta piperita, regaliz

La Fitoterapia hoy: evidencias

II Congreso de Alimentación, Nutrición y Dietética. Avances en Nutrición y Dietética Clínica: Prevención, Tratamiento y Gestión - Rol del Dietista-Nutricionist

La Fitoterapia hoy: evidencias

II Congreso de Alimentación, Nutrición y Dietética. Avances en Nutrición y Dietética Clínica: Prevención, Tratamiento y Gestión - Rol del Dietista-Nutricionist

Comportamiento al cultivo experimental en Aragón de Lippia alba para la normalización de la materia prima obtenida

Dentro de un amplio proyecto de normalización de productos naturales obtenidos de especies de la flora aromática latinoamericana, en el presente trabajo se presenta el estudio del comportamiento de los tres años iniciales de adaptación a cultivo de la especie Lippia alba (Fam. Verbenáceas), en diferentes condiciones agroclimáticas dentro la Comunidad Autónoma de Aragón. El material vegetal procedía de dos quimiotipos correspondientes a poblaciones de Uruguay y Costa Rica. La experimentación se realizó en parcelas de ensayo en Teruel y Ejea de los Caballeros (Zaragoza, España). La respuesta de adaptación a las zonas ensayadas ha sido diferente para cada uno de los quimiotipos estudiados. Ninguno de los dos ha soportado las condiciones agroclimáticas de la zona de Teruel, mientras que en Ejea de los Caballeros solamente se ha adaptado el quimiotipo Uruguay. El factor limitante ha sido fundamentalmente las bajas temperaturas durante los meses de invierno. Se ha realizado un seguimiento para cada espécimen de planta en cada una de las fases del cultivo con tratamiento individualizado planta a planta con el fin de comprobar su grado de selección. La biomasa producida ha proporcionado muestras normalizadas para comprobar su respuesta en materia seca y aceite esencial. El aceite esencial se ha obtenido por dos diferentes métodos de extracción, hidrodestilación y con dióxido de carbono supercrítico. Asimismo, se ha efectuado la analítica de los extractos obtenidos de las plantas en estudio. Químicamente, se ha confirmado el mantenimiento de las características del quimiotipo adaptado al cultivo en Aragón

Ethnopharmacological and Chemical Characterization of Salvia Species Used in Valencian Traditional Herbal Preparations

In Valencia Region (Spain), some wild and cultivated sages are used for medicinal purposes. Among them, Salvia officinalis subsp. lavandulifolia (SL) is widely employed and known for production of Spanish sage oil and herbal products. Nevertheless, it shares the market with S. blancoana subsp. mariolensis (SB) and, to a lesser extent, with their hybrid S. x hegelmaieri (SH). The knowledge on these two species is far low and confusion between them is possible. The aim of the present paper is to improve the ethnopharmacological, morphological and chemical knowledge of these sages, and to contribute to setting up quality specifications for improving identification and distinction from other Salvia species, such as, S. officinalis subsp. officinalis, S. x auriculata and S. microphylla var. microphylla. Samples were collected in Valencia Region and surrounding mountain areas during the ethnopharmacological field work. Twenty-nine medicinal uses were reported for SL, 13 of them being also recorded for SB. Of particular interest is a homemade liquor, used as digestive and known as “salvieta,” which is mainly prepared with SB. The macro- and microscopic characters are insufficient for identification of cut, crushed or powdered material. The study of the essential oil and a HPTLC (High Performance Thin Layer Chromatography) fingerprint of their extracts could help to distinguish SB from the other sages. The essential oil from dried aerial parts of SB (content: 1.8–4.5%) was characterized by GC-FID (Gas Chromatography with Flame Ionization Detector) and GC-MS (Gas Chromatography coupled to Mass Spectrometry) showing a composition close to that currently accepted for Spanish sage essential oil in the European Pharmacopoeia, ISO (International Standard Organization) and UNE (Una Norma Española) standards, with 1,8-cineole (13.7–45.7%) and camphor (12.1–28.6%) as major constituents. HPTLC methods, based on the analysis of hydroalcoholic and dichloromethane extracts, allowed to distinguish SB from other Salvia taxa currently found in Valencia region, except from its hybrid SH. This interdisciplinary study, that combines popular knowledge with botany and chemistry, allows to identify the raw herbal material from SB and to distinguish it from other Salvia species, ensuring a proper commercialization as herbal teas or for the preparation of spirits.This work had the financial support through the Borsa d'Estudi Països Catalans of the Institute for Catalan Studies to VM, and the financial support from an ERASMUS Internship (FsTRASBO48) to EH

Reduced Self-Perception of Fatigue after Intake of Panax ginseng Root Extract (G115®) Formulated with Vitamins and Minerals An Open-Label Study

Background: Unexplained fatigue is a common complaint. When underlying disease causes have been eliminated, lifestyle measures and supplementation can be indicated. Elaborating on clinical findings that G115®, a dry extract from the root of Panax ginseng, combined with vitamins and minerals could alleviate fatigue, this open label study aimed at assessing its effect on perceived fatigue and energy. Methods: Healthy adults self-reporting fatigue (n = 103) completed the Multidimensional Fatigue Inventory questionnaire. They rated their perceptions of mental and physical fatigue, energy, performance, and stress at baseline and 15, 30, 60 and 90 days after a daily intake of 40 mg G115® formulated with vitamins and minerals. Results: Compared with baseline values, mean self-perception of general fatigue was reduced by −7.55 units [95% CI: −8.44; −6.66] (−41.8%, p < 0.0001) at 90 days. All assessed perception ratings (mental and physical fatigue, reduced activity and motivation, performance, and stress) were significantly and steadily improved from two weeks after supplementation up to study's end. Overall satisfaction with the ability of the product to reduce fatigue reached 85% at Day 90. Conclusion: Daily intake with G115® extract formulated with vitamins and minerals suggests an improvement of self-perception of fatigue and energy in a fatigued adult population

Ethnopharmacological and chemical characterization of Salvia species used in Valencian traditional herbal preparations

In Valencia Region (Spain), some wild and cultivated sages are used for medicinal purposes. Among them, Salvia officinalis subsp. lavandulifolia (SL) is widely employed and known for production of Spanish sage oil and herbal products. Nevertheless, it shares the market with S. blancoana subsp. mariolensis (SB) and, to a lesser extent, with their hybrid S. x hegelmaieri (SH). The knowledge on these two species is far low and confusion between them is possible. The aim of the present paper is to improve the ethnopharmacological, morphological and chemical knowledge of these sages, and to contribute to setting up quality specifications for improving identification and distinction from other Salvia species, such as, S. officinalis subsp. officinalis, S. x auriculata and S. microphylla var. microphylla. Samples were collected in Valencia Region and surrounding mountain areas during the ethnopharmacological field work. Twenty-nine medicinal uses were reported for SL, 13 of them being also recorded for SB. Of particular interest is a homemade liquor, used as digestive and known as 'salvieta,' which is mainly prepared with SB. The macro- and microscopic characters are insufficient for identification of cut, crushed or powdered material. The study of the essential oil and a HPTLC (High Performance Thin Layer Chromatography) fingerprint of their extracts could help to distinguish SB from the other sages. The essential oil from dried aerial parts of SB (content: 1.8-4.5%) was characterized by GC-FID (Gas Chromatography with Flame Ionization Detector) and GC-MS (Gas Chromatography coupled to Mass Spectrometry) showing a composition close to that currently accepted for Spanish sage essential oil in the European Pharmacopoeia, ISO (International Standard Organization) and UNE (Una Norma Española) standards, with 1,8-cineole (13.7-45.7%) and camphor (12.1-28.6%) as major constituents. HPTLC methods, based on the analysis of hydroalcoholic and dichloromethane extracts, allowed to distinguish SB from other Salvia taxa currently found in Valencia region, except from its hybrid SH. This interdisciplinary study, that combines popular knowledge with botany and chemistry, allows to identify the raw herbal material from SB and to distinguish it from other Salvia species, ensuring a proper commercialization as herbal teas or for the preparation of spirits