Dynamic analysis of a long span, cable-stayed freeway bridge using NASTRAN

The dynamic analysis for earthquake- and wind-induced response of a long span, cable-stayed freeway bridge by NASTRAN in conjunction with post-processors is described. Details of the structural modeling, the input data generation, and numerical results are given. The influence of the dynamic analysis on the bridge design is traced from the project initiation to the development of a successful earthquake and wind resistant configuration

Structural modeling for control design (articulated multibody component representation)

High gain, high frequency flexible responses in gimbaled multibody systems are discussed. Their origin and physical significance are described in terms of detailed mass and stiffness modeling at actuator/sensor interfaces. Guyan Reduction, Generalized Dynamic Reduction, inadequate mass modeling detail, as well as system mode truncation, are shown to suppress the high gain high frequency response and thereby lose system flexibility important for stability and performance predictions. Model validation by modal survey testing is shown to risk similar loss of accuracy. Difficulties caused by high frequency responses in component mode simulations, such as DISCOS, and also linearized system mode simulations, are described, and approaches for handling these difficulties are discussed

Studien zur sympathischen Ophthalmie: IV. Die antigene Wirkung der Augenpigmente

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Vesicle-Like Biomechanics Governs Important Aspects of Nuclear Geometry in Fission Yeast

It has long been known that during the closed mitosis of many unicellular eukaryotes, including the fission yeast (Schizosaccharomyces pombe), the nuclear envelope remains intact while the nucleus undergoes a remarkable sequence of shape transformations driven by elongation of an intranuclear mitotic spindle whose ends are capped by spindle pole bodies embedded in the nuclear envelope. However, the mechanical basis of these normal cell cycle transformations, and abnormal nuclear shapes caused by intranuclear elongation of microtubules lacking spindle pole bodies, remain unknown. Although there are models describing the shapes of lipid vesicles deformed by elongation of microtubule bundles, there are no models describing normal or abnormal shape changes in the nucleus. We describe here a novel biophysical model of interphase nuclear geometry in fission yeast that accounts for critical aspects of the mechanics of the fission yeast nucleus, including the biophysical properties of lipid bilayers, forces exerted on the nuclear envelope by elongating microtubules, and access to a lipid reservoir, essential for the large increase in nuclear surface area during the cell cycle. We present experimental confirmation of the novel and non-trivial geometries predicted by our model, which has no free parameters. We also use the model to provide insight into the mechanical basis of previously described defects in nuclear division, including abnormal nuclear shapes and loss of nuclear envelope integrity. The model predicts that (i) despite differences in structure and composition, fission yeast nuclei and vesicles with fluid lipid bilayers have common mechanical properties; (ii) the S. pombe nucleus is not lined with any structure with shear resistance, comparable to the nuclear lamina of higher eukaryotes. We validate the model and its predictions by analyzing wild type cells in which ned1 gene overexpression causes elongation of an intranuclear microtubule bundle that deforms the nucleus of interphase cells