Evaluation of Bond Failure Rate of Orthodontic Brackets Bonded with Green Gloo-Two Way Color Changes Adhesive: A Clinical Study

BACKGROUND: Bonding is an important step in fixed orthodontic mechanotherapy. Many new materials introduced an adhesive for bonding. This study was designed to evaluate the clinical bond failure rate of orthodontic brackets bonded with green glue: two way color changes adhesive and transbond XT adhesive paste.METHODS: Eighteen male patients with a mean age of 16 years were included in the study. Convenience sampling technique was used to select the sample for this study. The split-mouth design was used to bond 360 brackets by one operator and both adhesives were used in each patient. Bond failure rates were estimated with respect to bonding procedure, dental arch, tooth type (incisor, canine, and premolar). The results were evaluated using the chisquare test. Kaplan – Meier analysis and the log rank test were used to estimate the survival rate of the brackets. Bracket failure rates for each system were analyzed, and failure causes as reported by the patients and the quadrant of each tooth in which bracketsfailed were recorded.RESULTS: The bond failure rate was 5.00% and 4.44% for green gloo and transbond XT group. No significant difference was found in the bond failure rate between transbond XT and Green gloo group. No significant difference was found in the bond failure rate between the two groups, in relation to right and left side and the type of teeth.CONCLUSION: Green gloo adhesive can be effectively used to bond orthodontic brackets

Deficiency of the dual ubiquitin/SUMO ligase Topors results in genetic instability and an increased rate of malignancy in mice

<p>Abstract</p> <p>Background</p> <p>Topors is a nuclear protein that co-localizes with promyelocytic leukemia bodies and has both ubiquitin and SUMO E3 ligase activity. Expression studies implicated Topors as a tumor suppressor in various malignancies. To gain insight into the function of Topors, we generated a Topors-deficient mouse strain.</p> <p>Results</p> <p>Mice homozygous for a mutant Topors allele exhibited a high rate of perinatal mortality and decreased lifespan. In addition, heterozygotes were found to have an increased incidence of malignancy, involving a variety of tissues. Consistent with this finding, primary embryonic fibroblasts lacking Topors exhibited an increased rate of malignant transformation, associated with aneuploidy and defective chromosomal segregation. While loss of Topors did not alter sensitivity to DNA-damaging or microtubule-targeting agents, cells lacking Topors exhibited altered pericentric heterochromatin, manifested by mislocalization of HP1α and an increase in transcription from pericentric major satellite DNA. Topors-deficient cells exhibited a transcriptional profile similar to that of cells treated with histone deacetylase inhibitors, and were resistant to the anti-proliferative effects of the histone deacetylase inhibitor trichostatin A.</p> <p>Conclusion</p> <p>These results indicate a unique role for Topors in the maintenance of genomic stability and pericentric heterochromatin, as well as in cellular sensitivity to histone deacetylase inhibitors.</p

Factors influencing demand for Islamic bank products in Sri Lanka

Purpose — The demand for Islamic banking products and services has been increasing globally over the past decade, including in Sri Lanka. However, the factors behind this surge are not yet fully understood. The aim of this research is to investigate the factors influencing the demand for Islamic banking products in Sri Lanka.Method — Primary data were collected through the distribution of questionnaires, which were distributed among 650 customers of Amana Bank PLC island-wide across its 33 branches. Convenience sampling techniques were employed for data collection. The Statistical Package for Social Sciences (SPSS) was utilized for analyzing the factors influencing the demand for Islamic banking products in Sri Lanka. Statistical methods, including regression analysis, correlation analysis, and a reliability test, were employed to analyze the collected data.Result — The findings indicate a robust relationship between factors such as religiosity, attitude, awareness, and reputation, and the demand for Islamic banking products in Sri Lanka. According to the regression analysis, awareness was identified as having the greatest impact on the demand for Islamic banking products, while reputation, attitude, and religiosity were determined to be the second, third, and least impactful factors, respectively.Novelty  — This research is pioneering in a country like Sri Lanka, where it is still in the developmental stage, and the majority of the population is non-Muslim. The findings offer valuable insights for policymakers in the Islamic banking and finance sectors, guiding them in formulating strategies to attract a broader customer base for their products and services among both Muslim and non-Muslim customers

The Camptothecin-Resistant Topoisomerase I Mutant F361S Is Cross-Resistant to Antitumor Rebeccamycin Derivatives. A Model for Topoisomerase I Inhibition by Indolocarbazoles

International audienceDNA topoisomerase I is a major cellular target for antitumor indolocarbazole derivatives (IND) such as the antibiotic rebeccamycin and the synthetic analogue NB-506 which is undergoing phase I clinical trials. We have investigated the mechanism of topoisomerase I inhibition by a rebeccamycin analogue, R-3, using the wild-type human topoisomerase I and a well-characterized recombinant enzyme, F361S. The catalytic activity of this mutant remains fully intact, but the enzyme is resistant to inhibition by camptothecin (CPT). Here we show that the mutated enzyme is cross-resistant to the rebeccamycin analogue. Despite their profound structural differences, CPT and R-3 interfere similarly with the activity of the wild-type and mutant topoisomerase I enzymes, and the drug-induced cleavable complexes are equally sensitive to the NaCl concentration. CPT and IND likely recognize identical structural elements of the topoisomerase I−DNA covalent complex; however, differences do exist in terms of sequence-specificity of topoisomerase I-mediated DNA cleavage. For the first time, a molecular model showing that CPT and IND share common steric and electronic features is proposed. The model helps to identify a specific pharmacophore for topoisomerase I inhibitors

Recyclability and catalytic characteristics of copper oxide nanoparticles derived from bougainvillea plant flower extract for biomedical application

This work aims to investigate the environmentally sustainable technique to synthesize the copper nanoparticles using bougainvillea flower ethanolic extract at ambient temperature. Copper nanoparticles have considerable potential for reducing the environment’s harmful pigments and nitrogen contaminants. The oxidized copper nanoscale catalysts are enclosed inside nanomaterial, which work as a benign and sustainable resource for capping agents. Ultraviolet spectroscopic, transmission electron microscopy (TEM), and X-ray crystallography (XRD) techniques were used to evaluate the produced oxidized copper nanocrystals. The particles produced have been very robust, are cylindrical in form, and have an outer diameter of 12 nm. Furthermore, under normal conditions, copper oxide (CuO) nanomaterials demonstrated strong photocatalytic efficiency in liquid media for the oxidation of Congo red, bromothymol blue, and 4-nitrophenol in an acidic solution acetic anhydride. Moreover, the CuO nanocrystalline enzyme could be readily vortexed or used for five cycles with an exchange rate of even over 90%. The evaporation process caused around 18% of the loss of weight between 25°C and 190°C, while soil organic breakdown caused almost 31% of the loss of weight around 700°C. As a result, the little reduction in enzymatic effectiveness of the recoverable multilayer CuO substrate might be attributed to catalytic degradation throughout spinning and processing

Stenosis of Carotid Arteries and Concentration of Some Blood Lipid Parameters amongst Tobacco Smokers in Abha: A Case–Control Study

Smoking is well known to be correlated with cardiovascular abnormalities, in particular atherosclerosis and heart diseases. This article investigates the effect and relationship of smoking tobacco on the thickness of the intima–media (IMT) belonging to the common carotid arteries (CCAs), and also blood concentration of the lipid profile (LP), mainly the total cholesterol, High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL) and also triglycerides. Nineteen male tobacco smokers and thirty-five healthy male non-smoking Saudi participants were involved in this study after obtaining their informed consent. An ultrasound and a spectrophotometer were used to determine the IMTs and lipid parameters, respectively. The thicknesses of the smokers’ right (RCA) and left carotid (LCA) arteries (0.72 and 0.7 mm, respectively) were significantly greater than the thicknesses of the arteries of the non-smokers (0.58 and 0.62 mm, respectively) (p-value = 0.005 and 0.04). Insignificant differences between the means of the other parameters in the two groups were studied. Smoking is a risk factor for stroke, because it significantly increases the IMTs of both the right and left carotid arteries