1,366 research outputs found
The Mechanism for Primordial Germ-Cell Migration Is Conserved between Japanese Eel and Zebrafish
Primordial germ cells (PGCs) are segregated and specified from somatic cells during early development. These cells arise elsewhere and have to migrate across the embryo to reach developing gonadal precursors. Several molecules associated with PGC migration (i.e. dead-end, nanos1, and cxcr4) are highly conserved across phylum boundaries. However, since cell migration is a complicated process that is regulated spatially and temporally by multiple adaptors and signal effectors, the process is unlikely to be explained by these known genes only. Indeed, it has been shown that there are variations in PGC migration pattern during development among teleost species. However, it is still unclear whether the actual mechanism of PGC migration is conserved among species. In this study, we studied the migration of PGCs in Japanese eel (Anguilla japonica) embryos and tested the migration mechanism between Japanese eel and zebrafish (Danio rerio) for conservation, by transplanting eel PGCs into zebrafish embryos. The experiments showed that eel PGCs can migrate toward the gonadal region of zebrafish embryos along with endogenous PGCs, even though the migration patterns, behaviors, and settlements of PGCs are somewhat different between these species. Our results demonstrate that the migration mechanism of PGCs during embryonic development is highly conserved between these two distantly related species (belonging to different teleost orders)
Search for the pentaquark via the reaction at 1.92 GeV/
The pentaquark baryon was searched for via the
reaction in a missing-mass resolution of 1.4 MeV/(FWHM) at J-PARC.
meson beams were incident on the liquid hydrogen target with the beam momentum
of 1.92 GeV/. No peak structure corresponding to the mass was
observed. The upper limit of the production cross section averaged over the
scattering angle of 2 to 15 in the laboratory frame was
obtained to be 0.26 b/sr in the mass region of 1.511.55 GeV/.The
upper limit of the decay width using the effective Lagrangian
approach was obtained to be 0.72 MeV/ and 3.1 MeV/ for
and , respectively.Comment: 5 pages, 3 figures, 1 tabl
The CrebA/Creb3-like transcription factors are major and direct regulators of secretory capacity
CrebA up-regulates expression of both the general protein machinery required in all cells for secretion and genes encoding cell type–specific secreted components
Toll-like receptor signaling adapter proteins govern spread of neuropathic pain and recovery following nerve injury in male mice.
BackgroundSpinal Toll-like receptors (TLRs) and signaling intermediaries have been implicated in persistent pain states. We examined the roles of two major TLR signaling pathways and selected TLRs in a mononeuropathic allodynia.MethodsL5 spinal nerve ligation (SNL) was performed in wild type (WT, C57BL/6) male and female mice and in male Tlr2-/-Tlr3-/-, Tlr4-/-, Tlr5-/-, Myd88-/-, Triflps2, Myd88/Triflps2, Tnf-/-, and Ifnar1-/- mice. We also examined L5 ligation in Tlr4-/- female mice. We examined tactile allodynia using von Frey hairs. Iba-1 (microglia) and GFAP (astrocytes) were assessed in spinal cords by immunostaining. Tactile thresholds were analyzed by 1- and 2-way ANOVA and the Bonferroni post hoc test was used.ResultsIn WT male and female mice, SNL lesions resulted in a persistent and robust ipsilateral, tactile allodynia. In males with TLR2, 3, 4, or 5 deficiencies, tactile allodynia was significantly, but incompletely, reversed (approximately 50%) as compared to WT. This effect was not seen in female Tlr4-/- mice. Increases in ipsilateral lumbar Iba-1 and GFAP were seen in mutant and WT mice. Mice deficient in MyD88, or MyD88 and TRIF, showed an approximately 50% reduction in withdrawal thresholds and reduced ipsilateral Iba-1. In contrast, TRIF and interferon receptor null mice developed a profound ipsilateral and contralateral tactile allodynia. In lumbar sections of the spinal cords, we observed a greater increase in Iba-1 immunoreactivity in the TRIF-signaling deficient mice as compared to WT, but no significant increase in GFAP. Removing MyD88 abrogated the contralateral allodynia in the TRIF signaling-deficient mice. Conversely, IFNβ, released downstream to TRIF signaling, administered intrathecally, temporarily reversed the tactile allodynia.ConclusionsThese observations suggest a critical role for the MyD88 pathway in initiating neuropathic pain, but a distinct role for the TRIF pathway and interferon in regulating neuropathic pain phenotypes in male mice
Measurement and comparison of individual external doses of high-school students living in Japan, France, Poland and Belarus -- the "D-shuttle" project --
Twelve high schools in Japan (of which six are in Fukushima Prefecture), four
in France, eight in Poland and two in Belarus cooperated in the measurement and
comparison of individual external doses in 2014. In total 216 high-school
students and teachers participated in the study. Each participant wore an
electronic personal dosimeter "D-shuttle" for two weeks, and kept a journal of
his/her whereabouts and activities. The distributions of annual external doses
estimated for each region overlap with each other, demonstrating that the
personal external individual doses in locations where residence is currently
allowed in Fukushima Prefecture and in Belarus are well within the range of
estimated annual doses due to the background radiation level of other
regions/countries
Measurements of the masses and widths of the and baryons
We present measurements of the masses and decay widths of the baryonic states
and using a data sample
corresponding to an integrated luminosity of 711 fb collected with the
Belle detector at the KEKB asymmetric-energy collider operating at
the resonance. We report the mass differences with respect to
the baryon MeV/, MeV/,
MeV/, MeV/, and the decay widths
MeV/,
MeV/,
MeV/,
MeV/,
where the first uncertainties are statistical and the second are systematic.
The isospin mass splittings are measured to be
MeV/ and
MeV/. These results are the most precise to date.Comment: 13 pages, 4 figures, Submitted to PRD(RC
Search for CP Violation in the Decay
We search for CP violation in the decay using a
data sample with an integrated luminosity of 977 fb collected with the
Belle detector at the KEKB asymmetric-energy collider. No CP violation
has been observed and the CP asymmetry in decay is
measured to be , which is the most sensitive
measurement to date. After subtracting CP violation due to
mixing, the CP asymmetry in decay is found to be
.Comment: 15 pages, 4 figures, 1 table. Published in JHE
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