Mineralogical studies of lunar meteorite Yamato-793169, a mare basalt

A preliminary mineralogical study of the lunar meteorites Yamato (Y)-793169 and Asuka (A)-881757,which were apparently derived from a mare region of the Moon, has been performed to identify crystallization trends of their pyroxenes. Y-793169 is a crystalline basalt with similar basaltic components to lunar breccias EET87521 and Y-793274,containing strongly zoned Fe-Ca-rich pyroxenes. Their zoning trends in the pyroxene quadrilateral are closest to those found in the basaltic clast in an Apollo 16 breccia. Differentiation trends expressed by Ti/(Ti+Cr) versus Fe/(Fe+Mg) of Y-793169 and A-881757 pyroxenes are similar but they differ from those of EET87521. The Y-793169 trend starts at a more Mg-rich composition point than the A-881757 trend. Based on differences in textures and ranges of zoning trends, the pyroxene of A-881757 could represent growth deeper in the lava unit under conditions more closely approaching equilibrium, than Y-793169,which appears to have formed from a lava flow of similar bulk composition. Although Y-793169 has been described as the VLT basalt, some mesostases contain significant amounts of ilmenite and ulvospinel, together with fayalite, troilite, chromite and a silica mineral. Mg-rich pyroxenes as found in Y-793274 and EET87521 are not present in Y-793169 and A-881757 basalts

Por Secretion System-Dependent Secretion and Glycosylation of Porphyromonas gingivalis Hemin-Binding Protein 35

The anaerobic Gram-negative bacterium Porphyromonas gingivalis is a major pathogen in severe forms of periodontal disease and refractory periapical perodontitis. We have recently found that P. gingivalis has a novel secretion system named the Por secretion system (PorSS), which is responsible for secretion of major extracellular proteinases, Arg-gingipains (Rgps) and Lys-gingipain. These proteinases contain conserved C-terminal domains (CTDs) in their C-termini. Hemin-binding protein 35 (HBP35), which is one of the outer membrane proteins of P. gingivalis and contributes to its haem utilization, also contains a CTD, suggesting that HBP35 is translocated to the cell surface via the PorSS. In this study, immunoblot analysis of P. gingivalis mutants deficient in the PorSS or in the biosynthesis of anionic polysaccharide-lipopolysaccharide (A-LPS) revealed that HBP35 is translocated to the cell surface via the PorSS and is glycosylated with A-LPS. From deletion analysis with a GFP-CTD[HBP35] green fluorescent protein fusion, the C-terminal 22 amino acid residues of CTD[HBP35] were found to be required for cell surface translocation and glycosylation. The GFP-CTD fusion study also revealed that the CTDs of CPG70, peptidylarginine deiminase, P27 and RgpB play roles in PorSS-dependent translocation and glycosylation. However, CTD-region peptides were not found in samples of glycosylated HBP35 protein by peptide map fingerprinting analysis, and antibodies against CTD-regions peptides did not react with glycosylated HBP35 protein. These results suggest both that the CTD region functions as a recognition signal for the PorSS and that glycosylation of CTD proteins occurs after removal of the CTD region. Rabbits were used for making antisera against bacterial proteins in this study

Structural Insights into the PorK and PorN Components of the Porphyromonas gingivalis Type IX Secretion System

The type IX secretion system (T9SS) has been recently discovered and is specific to Bacteroidetes species. Porphyromonas gingivalis, a keystone pathogen for periodontitis, utilizes the T9SS to transport many proteins including the gingipain virulence factors across the outer membrane and attach them to the cell surface via a sortase-like mechanism. At least 11 proteins have been identified as components of the T9SS including PorK, PorL, PorM, PorN and PorP, however the precise roles of most of these proteins have not been elucidated and the structural organization of these components is unknown. In this study, we purified PorK and PorN complexes from P. gingivalis and using electron microscopy we have shown that PorN and the PorK lipoprotein interact to form a 50 nm diameter ring-shaped structure containing approximately 32?36 subunits of each protein. The formation of these rings was dependent on both PorK and PorN, but was independent of PorL, PorM and PorP. PorL and PorM were found to form a separate stable complex. PorK and PorN were protected from proteinase K cleavage when present in undisrupted cells, but were rapidly degraded when the cells were lysed, which together with bioinformatic analyses suggests that these proteins are exposed in the periplasm and anchored to the outer membrane via the PorK lipid. Chemical cross-linking and mass spectrometry analyses confirmed the interaction between PorK and PorN and further revealed that they interact with the PG0189 outer membrane protein. Furthermore, we established that PorN was required for the stable expression of PorK, PorL and PorM. Collectively, these results suggest that the ring-shaped PorK/N complex may form part of the secretion channel of the T9SS. This is the first report showing the structural organization of any T9SS component

ハナス コト キク コト フレル コト カンジル コト フレラレル コト リンショウ ジッシュウ エ アンガジェ スル ホウホウ ノ ケントウ

理学療法学教育において臨床実習は重要な位置づけにある. しかし, その方法論は古くから変化しておらず, 現代の教育に必ずしも適当であるとはいえない. 今回, その課題について, 研究会を設立し検討した. その結果, 「関わること」の重要性が浮かび上がり, いかにして関われるのかを明確にする必要が示唆された. 研究会では人に関わる以前に自らに関わり, それを基盤に他者と関われると考えた. それは言語的コミュニケーションのみならず, 身体的コミュニケーションの重要性を前提としている. 関わりについてケア, クリティシズム, アンガージュマンなどのキーワードを使用し説明を加えた. 最終的に言語的・身体的コミュニケーションの経験を自らが解釈し, それを言語化できるような学習プロセスの経過(思考し表現すること)が必要であると結論づけた. 臨床実習は自己, 他者への関わりを理解し, 経験するプロセスなのである.Clinical training is the core of physical therapy education. However, its age-old methodology could be inappropriate for today\u27s education. We set up a study group and examined the issue. As a result, the importance of "participating with others" (i.e. interaction between instructors and students, and that between students and patients) came into focus and the necessity of exploring possible "participation" methods was indicated. We considered that one should be ready for participating with others by doing the same thing with oneself in advance. The idea was premised on the importance of physical communication as well as that of linguistic communication, and we discussed and tried to identify our concept of "participation" with keywords such as care, criticism or commitment (engagement). Finally we reached the conclusion that both instructors and students need a learning process, where they can interpret and verbalize (i.e. think and express) their experiences of linguistic and physical communication by themselves. Clinical training may be ultimately defined as a process of understanding what "participation" means and experiencing it

Application of next-generation sequencing to investigation of norovirus diversity in shellfish collected from two coastal sites in Japan from 2013 to 2014

A better understanding of the role played by shellfish regarding the manner of pathogen contamination, persistence, and selection may help considering epidemiology of noroviruses. Thus, norovirus genotype profiles in shellfish (Crassostrea gigas and Mitilus galloprovincialis) were investigated by using Next-generation sequencing (NGS) technology. In genogroup I (GI), 7 genotypes (abbreviated as GI.2 to GI.7, and GI.9) were detected from C. gigas, whereas 9 genotypes (GI.1 to GI.9) were detected from M. galloprovincialis. The genotype with the highest proportion found in both C. gigas and M. galloprovincialis was GI.4, and the second highest was GI.3. In genogroup II (GII), 17 genotypes (GII.1 to GII.9, GII.11 to GII.17, GII.21 and GI.22) were detected from C. gigas, whereas 16 genotypes (GII.1 to GII.8, GII.11 to GII.17, GII.21 and GI.22) were detected from M. galloprovincialis. The genotype with the highest proportion in both C. gigas and M. galloprovincialis was GII.4, the next highest differed between C. gigas and M. galloprovincialis. To our knowledge, this study may be the first trial to utilize the latest technology in this field, and reveal the diversity of norovirus genotypes present in shellfish