Hyperparathyroidism with presumed sellar-parasellar brown tumour based on imaging findings

Brown tumours are an end-stage complication of hyperparathyroidism. They are relatively rare today, due to earlier diagnosis and prompt treatment of hyperparathyroidism.1 Common locations are the mandible, pelvis, ribs and long bones of the axial skeleton.2 The skull base is an extremely rare site and, for obvious reasons, it is difficult to confirm the diagnosis in the absence of other lesions. In the face of a solitary lesion, a histological diagnosis of a ‘giant-cell tumour’ of benign nature should only be made once hyperparathyroidism has been actively excluded. Brown tumours, solid aneurysmal bone cysts, giant-cell reparative granuloma and giant cell tumour can all appear identical both macroand microscopically

Experimental studies and constitutive modelling of anelastic creep recovery during creep age forming

This paper presents a study of anelastic creep recovery during creep-ageing of an aluminium alloy AA7050-TAF. Uniaxial Creep-Ageing and Recovery Test (CART) was used to characterise the influence of anelastic creep strain on total creep deformation for determining the actual amount of springback in creep age forming (CAF) process. CART was performed on aluminium alloy AA7050-TAF at 174°C between the stress levels of 137.5 to 162.5 MPa. A constitutive model was developed for the prediction of the creep-ageing and recovery response of material in creep age forming. A 'back stress' variable was used to represent the net effect of the internal stresses of the material which causes anelastic creep recovery. Other microstructural variables were introduced to model complex micro-mechanisms and hardening effects including solid solution hardening, dislocation hardening, and age hardening. It has been found that the permanent strain after creep-ageing depends not only on total creep strain but also on anelastic strain. Simulation results from the constitutive model developed in this study show a good agreement with experimental data

Effects of asymmetric creep-ageing behaviour on springback of AA2050-T34 after creep age forming

This study investigates the effects of asymmetric creep-ageing behaviour on springback of aluminium alloys during creep age forming (CAF) process. An Al-Cu-Li alloy, AA2050, which shows an apparent asymmetric tension and compression creep-ageing behaviour is used for investigation. Several CAF trial tests to form singly-curved AA2050 plates with different thicknesses (3, 5 and 8 mm) are carried out with a four point bending setup. Meanwhile, two sets of finite element (FE) models of corresponding processes have been developed, in which either the conventional symmetric or the new asymmetric creep-ageing behaviour of the alloy was used. The asymmetric models can well predict the shape of formed plates for thicker materials (5 and 8 mm), while symmetric models provide over-prediction of final deflections. The results from asymmetric models indicate that more creep strain is generated in the tension stressed part of the forming plate than that in the other part with compressive stresses and therefore, resulting in an asymmetric distribution of the relaxed stresses through the thickness of the forming plates after creep-ageing. After springback, both top and bottom surfaces of the formed plates show significant compressive stresses while tensile residual stresses exist in the centre of the formed plates. The work in this study helps to understand the particular springback behaviour of AA2050 with asymmetric creep-ageing behaviour in CAF, and can be used to guide future industrial applications of the alloy in the CAF process

Voltage Sensor Probes (VSPs) as an Efficient Tool to Screen for Inhibitors of Voltage-Gated Sodium Channels

Voltage-gated sodium channels (Nav) represent a therapeutically validated group of targets for the development of antiepileptic drugs, analgesics and antiarrhythmics [1]. However most of the existing drugs acting as Nav blockers suffer from multiple side effects, but the existence of a multigene family of Nav [2] suggests that the identification of new compounds that selectively block Nav isoforms might have better therapeutic efficiency and reduced side effects. Due to their molecular interference with numerous ion channels, alkaloids represent a group of natural products of particular interest. This is the reason why we have evaluated the efficiency of an in-house method to screen a library of isoquinoline alkaloids formerly isolated in our laboratory. Mammalian GH3 cells constitutively expressing Nav where used in conjunction with Voltage Sensor Probes (VSPs), the signals being read on a fluorescence plate reader. Thanks to this technique, we were able to precisely detect Nav channels activators or blockers. Among 62 compounds tested, 5 isoquinolines appeared as potent Nav channels inhibitors. References: 1. Salat, K. et al. (2014) EOID 23:1093-1104 2. Yu, F.H. et al (2003) Genome Biol. 4

Spectral fingerprint of quantum confinement in single CsPbBr3_3 nanocrystals

Lead halide perovskite nanocrystals (NCs) are promising materials for classical and quantum lightemission applications. To gain a better understanding of their outstanding properties, a thorough understanding of the band-edge exciton emission is needed which is not reachable in ensemble and room temperature studies because of broadening effects. Here, we report on a study of the photoluminescence (PL) of single CsPbBr$_3$ NCs in the intermediate quantum confinement regime at cryogenic temperature. We reveal the size-dependence of the spectral features observed in single NCs PL: the bright-triplet exciton energy splittings, the trion and biexciton binding energies as well as the optical phonon replica spectrum. In addition, we show that the bright triplet energy splittings are consistent with a pure exchange model and that the variety of polarisation properties and PL spectra of single CsPbBr$_3$ NCs can be simply rationalised by considering the orientation of the emitting dipoles and the thermal populations of the emitting states

LNK (SH2B3): paradoxical effects in ovarian cancer.

LNK (SH2B3) is an adaptor protein studied extensively in normal and malignant hematopoietic cells. In these cells, it downregulates activated tyrosine kinases at the cell surface resulting in an antiproliferative effect. To date, no studies have examined activities of LNK in solid tumors. In this study, we found by in silico analysis and staining tissue arrays that the levels of LNK expression were elevated in high-grade ovarian cancer. To test the functional importance of this observation, LNK was either overexpressed or silenced in several ovarian cancer cell lines. Remarkably, overexpression of LNK rendered the cells resistant to death induced by either serum starvation or nutrient deprivation, and generated larger tumors using a murine xenograft model. In contrast, silencing of LNK decreased ovarian cancer cell growth in vitro and in vivo. Western blot studies indicated that overexpression of LNK upregulated and extended the transduction of the mitogenic signal, whereas silencing of LNK produced the opposite effects. Furthermore, forced expression of LNK reduced cell size, inhibited cell migration and markedly enhanced cell adhesion. Liquid chromatography-mass spectroscopy identified 14-3-3 as one of the LNK-binding partners. Our results suggest that in contrast to the findings in hematologic malignancies, the adaptor protein LNK acts as a positive signal transduction modulator in ovarian cancers

Sulfur isotopic compositions of individual organosulfur compounds and their genetic links in the Lower Paleozoic petroleum pools of the Tarim Basin, NW China

During thermochemical sulfate reduction (TSR), H2S generated by reactions between hydrocarbons and aqueous sulfate back-reacts with remaining oil-phase compounds forming new organosulfur compounds (OSCs) that have similar δ34S values to the original sulfate. Using Compound Specific Sulfur Isotope Analysis (CSSIA) of alkylthiaadamantanes (TAs), alkyldibenzothiophenes (DBTs), alkylbenzothiophenes (BTs) and alkylthiolanes (TLs), we have here attempted to differentiate OSCs due to primary generation and those due to TSR in oils from the Tarim Basin, China. These oils were generated from Cambrian source rocks and accumulated in Cambrian and Ordovician reservoirs. Based on compound specific sulfur isotope and carbon isotope data, TAs concentrations and DBT/phenanthrene ratios, the oils fall into four groups, reflecting different extents of source rock signal, alteration by TSR, mixing events, and secondary generation of H2S. Thermally stable TAs, that were produced following TSR, rapidly dominate kerogen-derived TAs at low to moderate degrees of TSR. Less thermally stable TLs and BTs were created as soon as TSR commenced, rapidly adopted TSR-δ34S values, but they do not survive at high concentrations unless TSR is advanced and ongoing. The presence of TLs and BTs shows that TSR is still active. Secondary DBTs were produced in significant amounts, sufficient to dominate kerogen-derived DBTs, only when TSR was at an advanced extent. The difference in sulfur isotopes between (i) TLs and DBTs and (ii) BTs and DBTs and (iii) TAs and DBTs, represents the extent of TSR while the presence of TAs at greater than 20 μg/g represents the occurrence of TSR. The output of this study shows that compound specific sulfur isotopes of different organosulfur compounds, with different thermal stabilities and formation pathways, not only differentiate between oils of TSR and non-TSR origin, but can also reveal information about relative timing of secondary charge events and migration pathways