Malondialdehyde levels and total antioxidant capacity in the dental follicles of the asymptomatic impacted third molars

Objectives: Malondialdehyde (MDA), is one of many low molecular weight end products of lipid peroxidation (LPO), increases in oxidative stress. Antioxidants such as total antioxidant capacity (TAC) have a protective effect against reactive oxygen species. The aim of this study is to examine the development of the antioxidant defense mechanism in dental follicles (DF) of radiologically asymptomatic impacted third molars (ITM) by using MDA and TAC.Materials and Methods: This study involved 40 DF of 40 patients referred for clinically and radiographically asymptomatic ITM. 40 healthy gingival tissues in the same patients obtained during surgical removal of teeth as a control group. This study involved DF widths on periapical radiographs narrower than 2.5 mm were included in the study. All of tissues samples were analyzed for MDA and TAC.Results: Levels of the MDA and TAC in DF were significantly higher than the levels of MDA and TAC provided from healthy gingival tissues of the same patients (p<0.05).Conclusions: The results of our study showed that an important antioxidant defense mechanism may also occur in DFs of asymptomatic ITM. In the light of these preliminary findings of the presented study, supplementary studies should be undertaken to establish the differences between inflammation affecting the DF and clinical outcomes

Live weight and body measurements of male and female native ducks raised in different raising systems

4th International Poultry Meat Congress -- APR 26-30, 2017 -- Antalya, TURKEYWOS: 000431308500001The purpose of this study was to determine live weight and body measurements of male and female native ducks raised in different raising systems. One hundred and twenty native ducks (60 males, 60 females) were used in the study. The ducks were raised in deep litter floor and cage systems. Live weight and body values were measured every two weeks, until they were 56 days old. Three-parameter logistic regression and Gompertz model were used to determine growth model of male and female ducks. Interactions of time-raising system and time-sex were statistically significant in terms of live weight. At the end of eight weeks, live weights of ducks raised in deep litter floor were higher than ducks raised in cage system. In addition, live weights of male ducks were higher than female ducks. Consequently, deep litter floor is more appropriate for live weight in native ducks. Accuracy rate of Three-parameter Logistic and Gompertz models for estimation of growth in ducks was between 0.91-0.95 and similar results were obtained from both models. The Gompertz model is appropriate for the data structure of this study because it contains fewer iterations than the Three-Parameter Logistic model.Scientific Research Project Council of Kafkas University [2011-VF-03]This study was financially supported by Scientific Research Project Council of Kafkas University (Project number: 2011-VF-03). This study was delivered as an oral presentation in the 4th International Poultry Meat Congress (26-30 April, 2017)

Estimation of live weights at the beginning and the end of grazing season in Tuj lambs via scores of factor analysis

WOS: 000437083800006This study was conducted in order to eliminate multicollmeanty between body measurements of Tuj lambs and to estimate their live weights in the beginning and the end of grazing season (GS) using factor analysis scores, which were determined by calculating some body measurements, in multiple regression model. For this purpose, the matenal of the study consisted of 297 Tuj lambs born between 2009 and 2013. Live weights (LW) of lambs in the beginning and at the end of GS and body length (BL), withers height (WH), chest girth (CG), chest depth (CD), circumferences of cannon forelimb (CCF) and circumferences cannon hmdlimb (CCH) measurements were analyzed m the study. Factor analysis were used to determine the estimated equation between the characteristics. It was determined that there was a multicollinearity between, CCF and CCH measured at the beginning of GS. Problem of multicollinearity between independent vanables was eliminated by using factor scores (FS) determined by factor analysis in multiple regression models. Consequently, two factors were detected from results of factor analysis applied to body measurements to determine live weights of Tuj lambs m the beginning and at the end of GS. These factors for LW m the beginning and at the end of GS were found to explain 84 8% and 78 9% of total variance, respectively. Thereby, it was revealed that more accurate estimations could be made by using factor analysis scores m estimating live weights of Tuj lambs at the beginning and the end of GS. In addition, it was concluded that applying factor analysis scores denved from independent vanables decreased mistake m the model according to least squares method

Relationship between seropositivity of Encephalitozoon cuniculi and renal biochemical markers in clinically healthy rabbits

WOS: 000471803700012Encephalitozoon cuniculi can cause latent disease, especially in lagomorphs and many wild and domestic animals in various countries. This infection is important for veterinary and public health because it is caused by a potentially zoonotic and opportunistic pathogen. The aim of this study was to investigate the relationship between seropositivity of E. cuniculi and renal function markers, which may be useful in predicting the disease in clinically healthy rabbits. In this study, the seropositivity of E. cuniculi infection in clinically healthy rabbits was determined, and necropsy fmdings were evaluated along with the results of renal function tests. In a laboratory rabbit breeding facility, enzyme-linked immunosorbent assay (ELISA) tests showed that 48 (49.5%) of 97 rabbits were seropositive against E. cuniculi. Blood urea nitrogen (BUN) and creatinine levels were significantly elevated in seropositive animals. Two seropositive rabbits were necropsied to confirm the infection. According to histopathological findings in the kidney, degenerative changes and E. cuniculi spores were identified in the tubule epithelia. Serum creatinine (p<0.001) and BUN (p<0.01) levels were found to have a statistically significant relationship with the serological status of rabbits. Serological and histopathological methods are not routinely used in rabbits to diagnose E. cuniculi infection. The kidneys were one of the most affected organs in encephalitozoonosis in rabbits. As revealed in this study, the testing blood urea nitrogen and creatinine levels would be useful for the evaluation of general health status and renal function of the seropositive rabbits, and clinical interpretation as well

Immunohistochemical investigation of cerebellum in dogs infected with canine distemper virus

The cerebella of 21 dogs with canine distemper virus (CDV) infection and four normal dogs were examined histopathologically and immunohistochemically. Cerebella of CDV-infected dogs showed nonsuppurative demyelinating encephalomyelitis, classified as acute, subacute or chronic. Immunolocalisation of CDV antigen also confirmed the infection. Tissues were examined for co-localisation of the CDV antigen with either an astrocyte-specific marker, glial fibrillary acidic protein (GFAP), or an oligodendrocyte-specific marker, galactocerebroside (GalC). Immunoreactive cells were counted in demyelinating areas of the white matter. The number of astrocytes (GFAP positive) was significantly (p < 0.05) higher in CDV-infected dogs compared to controls. In contrast, the number of oligodendrocytes (GalC positive) was significantly (p < 0.001) lower in CDV-infected dogs and was much lower in chronic cases (p < 0.05). Approximately 41% of astrocytes and 17% of oligodendrocytes were immunoreactive for CDV. The ratio of CDV-infected oligodendrocytes and astrocytes remained almost constant during the progression of the disease (P ≯ 0.05). In conclusion, CDV infects both astrocytes and oligodendrocytes. The gradual loss of oligodendrocytes is most likely responsible for the progressive demyelination in CDV infection. Astrocytosis in CDV infection should be further investigated if it occurs to stimulate oligodendrocytes for myelin production to compensate for the loss or to induce oligodendrocyte degeneration

Seroprevalence of Neospora caninum in dairy cattle ranches with high abortion rate: Special emphasis to serologic co-existence with Toxoplasma gondii, Brucella abortus and Listeria monocytogenes

YILDIZ, Kader/0000-0001-5802-6156; Celebi, Bekir/0000-0002-4545-5573; Babur, Cahit/0000-0002-6524-3260WOS: 000271052900029PubMed: 19592171The aim of this study was to determine seroprevalence of Neospora caninum in dairy cattle ranches with higher abortion rates and co-existence of Neospora caninum, Toxoplasma gondii, Brucella abortus, and Listeria monocytogenes antibodies. The blood samples were collected from dairy cows with history of abortion (n = 234) as well as from pregnant cows that were (n = 323) kept in the same ranches. N. caninum seroprevalence was 10.77% (60/557). The co-existence rate of N. caninum seropositivity with T. gondii, B. abortus and L. monocytagenes was 24.77% (138/557), 13.82% (77/557) and 42.85% (162/378), respectively. Only one animal had significant antibody titers for all analyzed infectious agents. The seroprevalence of N. caninum and T. gondii were significantly higher in pregnant cows than aborted cows (p < 0.05 and p < 0.001, respectively). As a result, the comprehensive data generated through this study can significantly contribute to understanding of serologic association of N. caninum with T gondii, B. abortus and L monocytogenes in pregnant and aborted dairy cows. (C) 2009 Elsevier B.V. All rights reserved.Kirikkale University Research CouncilKirikkale University [2005/16]This work was supported by a grant (project no: 2005/16) from the Kirikkale University Research Council

Base study for the establishment of national Salmonella control program in hatching farms and table eggs in Turkey

WOS: 000523562000023Foodborne infections due to Salmonella are still a major concern worldwide. Particularly contaminated egg and egg related products are the primary sources for human salmonellosis. It is necessary to determine the risk factors associated with Salmonella contamination of eggs within the scope of farm to table and environment. The objective of this study was to develop the "National Salmonella Control Program in Laying Hens" and report the prevalence and serotype distribution findings of Salmonella in laying hens and eggs in Turkey. A total of 2122 samples were collected and analysed according to ISO 6579:2002 after the isolation and identification procedures. All Salmonella isolates were serotyped including 726 eggs and 1396 farm specimens from 241 epidemiological units (EpUs) that were located in 9 different provinces between 2015 and 2017. Salmonella contamination was detected in 14.9% of 241 EpUs. The results indicated that almost half of the flocks have multiple contamination sources. The highest contamination rate was obtained from environmental (11%) followed by faeces (7.5%) and the lowest was from water samples (1.6%). The overall contamination rate was detected as 7.46% for farms and 3.3% for eggs. As S. Enteritidis and S. Typhimurium are the most frequently seen serotypes all over the world, in Turkey S. Typhimurium was not detected and S. Enteritidis was the 5th most common isolated serotype. According to our results it can be concluded that differences in various countries, particularly geographical and egg hatching systems, may affect the contamination rate and serotype distribution of Salmonella.Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113R036, 113R037]The part of this study was supported by a grant of The Scientific and Technological Research Council of Turkey (TUBITAK-project numbers as; 113R036 and 113R037)

The relationship between seropositivity and tissue cysts in sheep naturally infected with Toxoplasma gondii

Gencay, Yilmaz Emre/0000-0002-2154-9663; YILDIZ, Kader/0000-0001-5802-6156; Babur, Cahit/0000-0002-6524-3260; ATMACA, HASAN TARIK/0000-0001-8379-4114WOS: 000331515700009Skeletal muscles (tongue, masseter, leg, intercostal, and diaphragmatic muscles) and brain samples of 100 sheep at slaughter were analysed for the presence of T. gondii tissue cysts along with serum IgG titres. Two methods of isolation by percoll gradient centrifugation and tissue microarray (TMA) technique with immunoperoxidase staining were used. Seropositivity was detected in 88% (88/100) of sheep sera analysed by indirect fluorescent antibody test. Tissue cysts were observed in 46 (52.3%, 46/88) and 15 (17%, 15/88) of the seropositive sheep with the isolation technique and TMA and immunoperoxidase staining, respectively. The diameters of the tissue cysts were 25-58 x 25-62 (mean 34 x 36) mu m. The relationship between the presence of tissue cysts and seropositivity in sheep was statistically significant at 1/16 (P < 0.01) and at 1/64 and 1/128 (P < 0.001) serum dilutions.TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG 110O497]This work was supported by a grant from TUBITAK (project no: TOVAG 110O497)

The effects of aflatoxin residues on nutritional contents in ground red chili peppers (Capsicum annuum)

WOS: 000435278400151