Estrategias de solución para análisis de consolidación acoplados en medios porosos

Se revisan brevemente las estrategias empleadas usualmente para resolver las ecuaciones acopladas del problema de la consolidación de suelos saturados. Luego se introduce un nuevo esquema basado en la solucion particionada del conjunto de ecuaciones acopladas y se desarrollan los criterios de convergencia y estabilidad necesarios.Peer Reviewe

Estrategias de solución para análisis de consolidación acoplados en medios porosos

Se revisan brevemente las estrategias empleadas usualmente para resolver las ecuaciones acopladas del problema de la consolidación de suelos saturados. Luego se introduce un nuevo esquema basado en la solucion particionada del conjunto de ecuaciones acopladas y se desarrollan los criterios de convergencia y estabilidad necesarios.Peer Reviewe

SURVEY METHODS FOR SEISMIC VULNERABILITY ASSESSMENT OF HISTORICAL MASONRY BUILDINGS

On 20th and 29th of May 2012, two powerful earthquakes struck northern Italy. The epicentres were recorded respectively in Finale Emilia (magnitude 5.9 Ml) and Medolla (magnitude 5.8 Ml) in the province of Modena, though the earthquake was formed by a series of seismic shakes located in the district of the Emilian Po Valley, mainly in the provinces of Modena, Ferrara, Mantova, Reggio Emilia, Bologna and Rovigo. Many monuments in the city of Mantova were hit by the earthquake and, among these, Palazzo Ducale with the well-known Castello di San Giorgio which host the noteworthy "Camera degli Sposi". This building, the most famous of the city, was so damaged that it was closed for more than one year after the earthquake. The emblem of the Palace and Mantova itself, the previously cited "Camera degli Sposi" realized by Andrea Mantegna, was damaged and all the economic and social life of the city was deeply affected. Immediately after the earthquake, the Soprintendenza per i Beni Architettonici e Paesaggistici of Brescia, Cremona and Mantova establish an agreement with the University Iuav of Venice, requiring an analysis and assessment of the damage in order to proceed with the development of an intervention project. This activity turned out to be very important not only from the point of view of the recovery of the architectural and artistic heritage but also because the city's economy is based primarily on tourism. The closure of one of the most important monuments of Mantova has led to a significant and alarming decline in the government income

Cytotoxic T cells expressing the co-stimulatory receptor NKG2 D are increased in cigarette smoking and COPD

<p>Abstract</p> <p>Background</p> <p>A suggested role for T cells in COPD pathogenesis is based on associations between increased lung cytotoxic T lymphocyte (CD8⁺) numbers and airflow limitation. CD69 is an early T cell activation marker. Natural Killer cell group 2 D (NKG2D) receptors are co-stimulatory molecules induced on CD8⁺T cells upon activation. The activating function of NKG2 D is triggered by binding to MHC class 1 chain-related (MIC) molecules A and B, expressed on surface of stressed epithelial cells. The aim of this study was to evaluate the expression of MIC A and B in the bronchial epithelium and NKG2 D and CD69 on BAL lymphocytes in subjects with COPD, compared to smokers with normal lung function and healthy never-smokers.</p> <p>Methods</p> <p>Bronchoscopy with airway lavages and endobronchial mucosal biopsy sampling was performed in 35 patients with COPD, 21 healthy never-smokers and 16 smokers with normal lung function. Biopsies were immunohistochemically stained and BAL lymphocyte subsets were determined using flow cytometry.</p> <p>Results</p> <p>Epithelial CD3⁺lymphocytes in bronchial biopsies were increased in both smokers with normal lung function and in COPD patients, compared to never-smokers. Epithelial CD8⁺lymphocyte numbers were higher in the COPD group compared to never-smoking controls. Among gated CD3⁺cells in BAL, the percentage of CD8⁺NKG2D⁺cells was enhanced in patients with COPD and smokers with normal lung function, compared to never-smokers. The percentage of CD8⁺CD69⁺cells and cell surface expression of CD69 were enhanced in patients with COPD and smokers with normal lung function, compared to never-smokers. No changes in the expression of MIC A or MIC B in the airway epithelium could be detected between the groups, whereas significantly decreased soluble MICB was detected in bronchial wash from smokers with normal lung function, compared to never-smokers.</p> <p>Conclusions</p> <p>In COPD, we found increased numbers of cytotoxic T cells in both bronchial epithelium and airway lumen. Further, the proportions of CD69- and NKG2D-expressing cytotoxic T cells in BAL fluid were enhanced in both subjects with COPD and smokers with normal lung function and increased expression of CD69 was found on CD8⁺cells, indicating the cigarette smoke exposure-induced expansion of activated cytotoxic T cells, which potentially can respond to stressed epithelial cells.</p

SURVEY, DIAGNOSTICS, MONITORING METHODOLOGY AND DIGITAL TWIN FOR THE CONSERVATION OF THE FACADE OF THE CHURCH OF SANTA MARIA DI NAZARETH IN VENICE

This contribution aims to illustrate the experimental research linked to the restoration concluded in 2018 for the conservation of the façade of the Santa Maria di Nazareth church in Venice and the ongoing monitoring methodology for the evaluation of its conservation state over time. The baroque façade is unique in Venice as it is entirely made of Carrara marble, a limestone with a saccharoid structure which has proved over the centuries unsuitable for the aggressive lagoon climate, given the complex architectural conformation and the unfavourable environmental conditions in which the façade is inserted.The state of conservation of the marble at the beginning of the last worksite showed widespread and worrying degradation in many parts, especially in the more protruding ones: in fact, the stone surface reached detachment and pulverization by simple contact. The ultimate goal was to achieve a compatible and retractable conservative restoration for a possible improvement of the conservative results over time and also to facilitate an effective retrieval of scientific data in case of future interventions. With this aim, once the construction site was completed, a survey campaign with the purpose of reproducing a digital twin through 3D modelling was planned, to monitor the façade to have an exhaustive knowledge of the possible vulnerabilities present, with the involvement of the VIDE laboratory of IUAV University of Venice. A data acquisition protocol has been developed for the preservation of cultural heritage, thus guaranteeing an uninterrupted knowledge of the material degradation and of the structural situation.</p

A methodology for the evaluation of morphology-based constitutive laws of corroded steel rebars

The assessment of degraded reinforced concrete (RC) existing structures is nowadays a topical problem since several ones have an age close to or higher than their design life. The degradation state of the structure needs to be properly assessed and included in the adopted numerical or analytical models. In this framework, rebars corrosion phenomena can modify the structural behavior of RC members, especially when the corrosive attack is localized in specific areas. As an example, in presence of localized concentrations of chlorides, pitting corrosion of the steel bars occurs, with a localized reduction of the bar section through pits. In this work, the results of a preliminary experimental survey performed on artificially corroded steel rebars are shown, to define an integrated methodology for the definition of material constitutive laws, accounting for morphological aspects of the corrosion phenomenon. A set of steel rebars, embedded in concrete prisms, have been artificially corroded with an accelerated process through electrolytic cells, to obtain local marked pits. The steel rebars have been extracted, cleaned, weighed and subjected to 3D scanning using two different techniques (one based on laser scanning and the other based on structured-light scanning) to evaluate the effective corrosion amount and morphology. Finally, tensile tests were carried out to evaluate the stress-strain curves and the values of yielding, ultimate strengths and elongation to fracture. The presented data and methodology can represent a useful reference for the definition of phenomenological constitutive laws for steel rebars affected by pitting corrosion

Perforin, granzyme B, and FasL expression by peripheral blood T lymphocytes in emphysema

<p>Abstract</p> <p>Background</p> <p>It is generally accepted that emphysematous lungs are characterized by an increase in the numbers of neutrophils, macrophages, and CD8⁺T lymphocytes, the lasts having increased cytotoxic activity. Because systemic inflammation is also a component of emphysema, we hypothesize that peripheral CD8⁺T lymphocytes of emphysematous smokers who show evidence of systemic inflammation will have higher expression of cytotoxic molecules.</p> <p>Methods</p> <p>We assessed parameters of systemic inflammation in normal individuals (smokers or non-smokers) and in emphysematous subjects with an active smoking history by measuring serum interleukine-6, C-reactive protein, and tumor necrosis factor. Expression of perforin, granzyme B, and FasL protein by CD8⁺T lymphocytes, CD4⁺T lymphocytes, and natural killer cells were assessed by flow cytometry while perforin, granzyme B, and FasL mRNA expression were measured on purified systemic CD8⁺T lymphocytes by real-time PCR.</p> <p>Results</p> <p>Emphysematous smokers had higher levels of serum interleukine-6 than normal subjects. Even with the presence of systemic inflammation in emphysematous smokers, the percentage of peripheral CD8⁺T lymphocytes, CD4⁺T lymphocytes, and NK cells expressing perforin and granzyme B protein was not different between the three groups.</p> <p>Conclusion</p> <p>Despite evidence of systemic inflammation, peripheral T lymphocytes of emphysematous smokers did not show higher levels of cytotoxic markers, suggesting that increase of activated T lymphocytes in the emphysematous lung may be due to either activation in the lung or specific peripheral recruitment.</p

Eosinophil and T Cell Markers Predict Functional Decline in COPD Patients

BACKGROUND. The major marker utilized to monitor COPD patients is forced expiratory volume in one second (FEV1). However, asingle measurement of FEV1 cannot reliably predict subsequent decline. Recent studies indicate that T lymphocytes and eosinophils are important determinants of disease stability in COPD. We therefore measured cytokine levels in the lung lavage fluid and plasma of COPD patients in order to determine if the levels of T cell or eosinophil related cytokines were predictive of the future course of the disease. METHODS. Baseline lung lavage and plasma samples were collected from COPD subjects with moderately severe airway obstruction and emphysematous changes on chest CT. The study participants were former smokers who had not had a disease exacerbation within the past six months or used steroids within the past two months. Those subjects who demonstrated stable disease over the following six months (ΔFEV1 % predicted = 4.7 ± 7.2; N = 34) were retrospectively compared with study participants who experienced a rapid decline in lung function (ΔFEV1 % predicted = -16.0 ± 6.0; N = 16) during the same time period and with normal controls (N = 11). Plasma and lung lavage cytokines were measured from clinical samples using the Luminex multiplex kit which enabled the simultaneous measurement of several T cell and eosinophil related cytokines. RESULTS AND DISCUSSION. Stable COPD participants had significantly higher plasma IL-2 levels compared to participants with rapidly progressive COPD (p = 0.04). In contrast, plasma eotaxin-1 levels were significantly lower in stable COPD subjects compared to normal controls (p < 0.03). In addition, lung lavage eotaxin-1 levels were significantly higher in rapidly progressive COPD participants compared to both normal controls (p < 0.02) and stable COPD participants (p < 0.05). CONCLUSION. These findings indicate that IL-2 and eotaxin-1 levels may be important markers of disease stability in advanced emphysema patients. Prospective studies will need to confirm whether measuring IL-2 or eotaxin-1 can identify patients at risk for rapid disease progression.National Heart, Lung, and Blood Institute (NO1-HR-96140, NO1-HR-96141-001, NO1-HR-96144, NO1-HR-96143; NO1-HR-96145; NO1-HR-96142, R01HL086936-03); The Flight Attendant Medical Research Institute; the Jo-Ann F. LeBuhn Center for Chest Diseas