Algorithm of optimal technology selection of broadband access network

High speed applications require the access network upgrading based on new optical technologies in “last mile”. At the planning stage of network modernization, special algorithms and techniques allow optimize and automate the designing process of network upgrade based on copper cables. However, these techniques are completely inapplicable if optical technologies are used to the access network modernization. Designed algorithm simplifies the throughput calculation of current access nodes and planning of new access nodes depending on subscriber’s requirementstto the broadband services. Developed program based on proposed algorithm allows to determinate access nodes optimal deployment as well as the required equipment characteristics

LACTOFERIN IN THE PROBLEM OF ANTI-INFECTIOUS PROTECTION OF BABIES IN THEIR FIRST YEAR OF LIVING

The author  summarizes  the  results  of research  of the  antibacterial,  antiviral  and  antifungal  properties  of multifunctional  human protein  — lactoferrin,  in order  to determine  the prospects  for its use in the prevention  and  treatment  of infectious  diseases  of children in their first year of life. The mechanisms of anti-infectious effect of this protein with breastfed children have been described. Basic differences between human lactoferrin and cattle lactoferrin have been shown. Biotechnology of obtaining recombinant human lactoferrin from the milk of genetically engineered dairy animals (goat-producers) has been described. According to the studies, both by physical and chemical parameters and biological activity, human lactoferrin, obtained from milk-producing  goats, corresponds to its natural counterpart

БИФИДОГЕННЫЕ СВОЙСТВА БИОТЕХНОЛОГИЧЕСКОГО АНАЛОГА ЛАКТОФЕРРИНА ЧЕЛОВЕКА

Background:  Recent research shows that the growth and development of the gastrointestinal  tract of children fed by breast milk is more intense than that of the formula fed, since the human lactoferrin contained in the breast milk is a factor that stimulates cell growth. Therefore, the possibility of using exogenous lactoferrin will be of great importance in the nutrition of infants.Objektive: To study the bifidogenic properties of the biotechnological analogue of human lactoferrin. Methods: Kinetics of growth and CFU titer of bifidobacterial culture in the presence of a biotechnological analogue of human lactoferrin (0,05–5 mg /ml) was determined.Results: It has been shown that different concentrations of the protein can have both a stimulating (for B. bifidum and B. infantis) and inhibitory (for B. longum) effect on the growth of bifidobacteria, which is due to the affinity of lactoferrin binding to them. It seems important to further study the stimulating effect of this protein on the growth of lactobacilli in the intestine of the child.Conclusion:  Due to bifidogenic and high bactericidal action, lactoferrin can be effective in feeding newborns.Обоснование.   Новейшие исследования  показывают,  что  рост  и развитие  желудочно-кишечного  тракта  детей, вскармливаемых  материнским молоком,  происходит  интенсивнее,  чем у вскармливаемых  молочными смесями, поскольку содержащийся в нем лактоферрин человека является фактором, стимулирующим клеточный рост. Именно поэтому возможность  использования экзогенного лактоферрина  будет иметь большую значимость в питании грудных детей.Цель  исследования — изучить бифидогенные  свойства биотехнологического аналога  лактоферрина человека.Методы. Определялась кинетика роста и КОЕ-титр культивирования бифидобактерий в присутствии биотехнологического  аналога лактоферрина человека.Результаты. Показано, что различные концентрации данного белка (0,05–5 мг/мл) могут оказывать как стимулирующее (для Bifidobacterium bifidum и Bifidobacterium infantis), так и ингибирующее (для Bifidobacterium longum) действие в отношении роста бифидобактерий,  что обусловлено аффинностью  связывания с ними лактоферрина. Представляется  важным дальнейшее изучение стимулирующего эффекта  этого белка на рост лактобацилл в кишечнике ребенка.Заключение. Благодаря  бифидогенному и выраженному бактерицидному действию лактоферрин может быть использован в лечебном питании новорожденных

ВОЗМОЖНОСТИ ПРИМЕНЕНИЯ ЛАКТОФЕРРИНА У ДЕТЕЙ ПЕРВОГО ГОДА ЖИЗНИ

The author  summarizes  the  results  of research  of the  antibacterial,  antiviral  and  antifungal  properties  of multifunctional  human protein  — lactoferrin,  in order  to determine  the prospects  for its use in the prevention  and  treatment  of infectious  diseases  of children in their first year of life. The mechanisms of anti-infectious effect of this protein with breastfed children have been described. Basic differences between human lactoferrin and cattle lactoferrin have been shown. Biotechnology of obtaining recombinant human lactoferrin from the milk of genetically engineered dairy animals (goat-producers) has been described. According to the studies, both by physical and chemical parameters and biological activity, human lactoferrin, obtained from milk-producing  goats, corresponds to its natural counterpart.В статье обобщены результаты исследований, посвященных изучению антибактериальных, противовирусных и противогрибковых свойств многофункционального белка человека — лактоферрина, для определения перспектив его применения с целью профилактики и лечения инфекционной патологии у детей первого года жизни. Описаны механизмы противоинфекционного действия этого белка у детей, находящихся на грудном вскармливании. Показаны основные различия между лактоферрином человека и лактоферрином крупного рогатого скота. Описана биотехнология получения рекомбинантного лактоферрина человека из молока генно-инженерных молочных животных (коз-продуцентов). Согласно проведенным исследованиям, как по физико-химическим параметрам, так и по биологической активности лактоферрин  человека, выделенный из молока коз-продуцентов, соответствует его природному аналогу

Morphological criteria for prognosis in patients with melanoma

Among the morphological criteria of prognosis in patients with melanoma of the skin the most important ones are thickness, depth of invasion, ulceration of the tumor, the presence of microsatellites and metastases in regional lymph nodes, the severity of the proliferative activity of tumor cell invasion into blood vessels. The value of clinical, anatomical and cell-type variant of melanoma, the degree of pigmentation, the severity of her infl ammatory infi ltration, fi broplasia, signs of regression is controversial

THE MOLECULAR MECHANISM OF LACTOFERRIN INFLUENCE ON BONE FORMATION

In present critical review of systematized materials on the breakthrough achievements of the last decade - the discovery of the effect of protein lactoferrin (LF) on bone formation. It is shownthat LF increases the number of osteoblasts, stimulate their proliferation and differentiation, and prevents their destruction. Action of LF exceeds that of many other previously established bone-forming factors. LF increases the ability of osteoblasts to synthesize and mineralize bone matrix. Apparently, the effect of LF on bone anabolism ensured by the presence of specific receptors on osteoblasts. It was found that LF also inhibits the formation of osteoclasts. Experimental studies have demonstrated that LF prevents the destruction of bone tissue in ovariectomizedanimals and, thus, developing the type of postmenstrual osteoporosis in women. We get the first clinical studies demonstrating an increase in the period of healing of bone injuries while reducing the level of endogenous LF. Since molecular research establishes that the expression of the LF gene is regulated by estrogen, which reduces the development of postmenopausal osteoporosis (PMO) in women, there is a need to further investigate the relationship of these processes, which will help to create a basis for the management of bone formation

Synthesis of novel azolo[1,2,3,5]thiatriazines

The cycloaddition of diazoazoles with acylisothiocyanates has been found to result in the formation of a 1,2,3,5-thiatriazine ring. This new reaction was used to prepare novel 8-substituted 4-[benzoylimino-4H-imidazo(pyrazolo)][5,1-d][1,2,3,5]thiatriazines. The structures of the compounds prepared were carefully studied by X-ray diffraction, IR and NMR spectroscopy

Major challenges in evaluating the quality of pharmaceutical substances (the assay)

Common topical issues of the quality assessment of pharmaceutical substances in terms of «Assay», are related to the compliance of actual test results to assay requirements as well as to the proper elaboration of normative documents (completeness, accuracy of the descriptions of analytical methods) and the appropriate test conditions validation with the materials in the scope of the mentioned regulatory documents. It was shown that an objective assessment of the quantitative content of an active ingredient in pharmaceutical substance of synthetic or mineral origin depends largely on the choice of the analytical method, use of the appropriate techniques considering the materials included in the monographs of the State Pharmacopoeia the Russian Federation and leading foreign pharmacopoeias

Development and Validation of Pomalidomide Determination in Human Plasma by HPLC-MS/MS Method

Introduction. B-cell malignancies of the plasma cell leads to the second most spread hematological malignancy disease, called multiple myeloma. Pomalidomide is used in case of previous multiple myeloma ineffective treatment. Pomalidomide is a thalidomide synthetic derived, approved as immunomodulatory drug by the Food and Drug Administration (FDA). Nowadays, detection of pomalidomide in blood plasma by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) is not spread. Moreover, the detection and the experimental setting accumulated data are varying greatly. This investigation provides development and validation of pomalidomide aiming to determine human blood plasma by HPLC-MS/MS method. The samples were processed by methanol protein precipitation.Aim. The aim of this study is to develop a method for the pomalidomide in human plasma by HPLC-MS/MS for pharmacokinetic studies.Materials and methods. Determination of pomalidomide in plasma by HPLC-MS/MS. The samples were processed by methanol protein precipitation.Results and discussion. This method was validated by next parameters: selectivity, matrix effect, calibration curve, accuracy, precision, spike recovery, lower limit of quantification, detection limit, carry-over and stability.Conclusion. The method of the determination of pomalidomide in human plasma was developed and validated by HPLC-MS/MS. The linearity in plasma sample was achieved in the concentration range of 1,00 – 500,00 ng/ml. Method could be applied to pomalidomide determination in plasma for PK and BE studies

BIFIDOGENIC PROPERTIES OF A BIOTECHNOLOGICAL ANALOGUE OF HUMAN LACTOFERRIN

Background:  Recent research shows that the growth and development of the gastrointestinal  tract of children fed by breast milk is more intense than that of the formula fed, since the human lactoferrin contained in the breast milk is a factor that stimulates cell growth. Therefore, the possibility of using exogenous lactoferrin will be of great importance in the nutrition of infants.Objektive: To study the bifidogenic properties of the biotechnological analogue of human lactoferrin. Methods: Kinetics of growth and CFU titer of bifidobacterial culture in the presence of a biotechnological analogue of human lactoferrin (0,05–5 mg /ml) was determined.Results: It has been shown that different concentrations of the protein can have both a stimulating (for B. bifidum and B. infantis) and inhibitory (for B. longum) effect on the growth of bifidobacteria, which is due to the affinity of lactoferrin binding to them. It seems important to further study the stimulating effect of this protein on the growth of lactobacilli in the intestine of the child.Conclusion:  Due to bifidogenic and high bactericidal action, lactoferrin can be effective in feeding newborns