Crystal Structure of the Homology Domain of the Eukaryotic DNA Replication Proteins Sld3/Treslin

SummaryThe initiation of eukaryotic chromosomal DNA replication requires the formation of an active replicative helicase at the replication origins of chromosomal DNA. Yeast Sld3 and its metazoan counterpart Treslin are the hub proteins mediating protein associations critical for the helicase formation. Here, we show the crystal structure of the central domain of Sld3 that is conserved in Sld3/Treslin family of proteins. The domain consists of two segments with 12 helices and is sufficient to bind to Cdc45, the essential helicase component. The structure model of the Sld3-Cdc45 complex, which is crucial for the formation of the active helicase, is proposed

紙上事例を用いた成人看護学看護過程演習の評価 (第3報) : 関連図を取り入れた演習における学生の自己評価の変化

報告Report成人看護学での紙上事例を用いた看護過程演習において、健康障害についての解釈・分析・統合の理解が深まるような演習方法の検討を行い、本学3年次学生に演習前後の自己評価調査を実施した。その結果、健康障害についての関連図作成をグループで取り組ませたことで自己評価得点の有意な上昇がみられた。しかし、演習後にも分析・統合に困難を感じる学生が2割程度みられ、基本的な健康査定や病態の変化など根拠をもとに自ら思考する能力を開発する指導方法についてさらに検討していく必要がある

The effect of DRAMA on estimating human nuro-receptor binding potential with PET

Objectives:FBP is a standard method for quantitative analysis in human neuroreceptor bindingassay, while OS-EM is used widely in tumor PET imaging. Owing to noise propagation from projection data, especially at high subset number, OS-EM leads to loss of signal to noise (SNR) and quantitative accuracy. A novel reconstruction algorithm DRAMA can suppress the noise propagation by using dynamic relaxation parameter with faster convergence compared to OSEM algorithm. In this study, we compared regional radioactivity concentrations and binding potential (BP) values between FBP, OS-EM and DRAMA to assess the effect of DRAMA algorithm on estimating human nuroreceptor binding. Methods: The PET scanning was carried out with Eminence Sophia(shimadzu corp.) . After 4min pre-injection transmission scan, [11C] FLB457, 90min dynamic PETscans were performed with 3D acquisition mode. To compare reconstruction parameter, a phantom scan was done with same scan protocol. Images were reconstructed with FBP, OSEM and DRAMA to assess quantitative accuracy and noise characteristics. Results: To evaluate the SNR, we performed the image quality tests along with NEMA NU-2 2007. Noise of DRAMA was 7% lower than that of FBP. On the other hands, Q10/N10 number of DRAMA was 5.3% higher than that of FBP,similarly, it is 3.5% higher than that of OS-EM. In patient study, the difference of BP by OSEM withiteration 4, subset 16 compared to FBP was+3.17% at maximum in the caudate, while that of DRAMA was +1.59%. Conclusions:There were no significant differences of BP values in thalamus,frontalcortex, temporal cortex and occipital cortex between DRAMA and FBP. Furthermore, noise propagation was not shown in DRAMA in phantom studies. These results indicated that DRAMA could provide similar performance of FBP with the improved SNR, therefore, DRAMA can be a novel standard reconstruction method of neuroreceptor PET studies.EANM\u2713 - Annual Congress of the European Association of Nuclear Medicin